<div><p>Hydrolytic enzyme activities were determined using L-leucine-4-methylcoumarinyl-7-amide (MCA) hydrochloride, 4-methylumbelliferyl α-D-glucopyranoside, and 4-methylumbelliferone (MUF) β-D-glucopyranoside (Sigma-Aldrich) as substrate proxies for leucine-aminopeptidase, α-glucosidase, and β-glucosidase activities, respectively. For each bottle and substrate proxy, 196 µL of unfiltered experimental or control water was added in duplicate to a pure-grade black 96-well plate (Brand Life Sciences) containing a single substrate proxy at saturation levels (final concentration 200 µM). Fluorescence (excitation 370 nm, emission 440 nm) was measured in a Tecan Infinite 200 Pro microplate reader immediately following the addition of the substrate and several more times over 7-20 h. The well plates were incubated in the dark at in situ temperature. MUF and MCA standard solutions prepared in seawater were used to determine hydrolysis rates. Killed controls (boiled sample water) and ultrapure water samples showed little change over the incubations.</p>
<p>See the table of time stamps under "Supplemental Documents", below.</p></div>
Bacterial cell counts during CDOM monoculture experiment
<div><p>This dataset is from a laboratory experiment. Four phytoplankton cultures and their associated bacterial communities were incubated in replicate roller bottles (1.9 L) over 3-6 weeks under laboratory conditions. Bacterial dynamics in the culture bottles were measured and correlated with geochemical parameters to determine the role of bacterial activities on the formation of CDOM in the cultures (Kinsey et al., 2018, see below).</p>
<p>The data include fluorescence and bacterial enzyme activity during CDOM monoculture experiments. The phytoplankton cultures were Skeletonema sp., Leptocylindrus sp., and Phaeocystis sp. Growth stages were initial, exponential, stationary, and degradation. </p></div>
Hydrolytic enzyme activity - other phytoplankton
<div><p>BCO-DMO Processing Notes:<br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- converted Excel file tables to a flat file and combined a-glu, b-glu, and leu substrate treatments.</p></div>
748423
Hydrolytic enzyme activity - other phytoplankton
2018-10-17T16:08:35-04:00
2018-10-17T16:08:35-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:748423
Hydrolytic enzyme activities during CDOM monoculture experiments with Skeletonema, Leptocylindrus, and Phaeocystis
This dataset is from a laboratory experiment. Four phytoplankton cultures and their associated bacterial communities were incubated in replicate roller bottles (1.9 L) over 3-6 weeks under laboratory conditions. Bacterial dynamics in the culture bottles were measured and correlated with geochemical parameters to determine the role of bacterial activities on the formation of CDOM in the cultures (Kinsey et al., 2018, see below).
The data include fluorescence and bacterial enzyme activity during CDOM monoculture experiments. The phytoplankton cultures were Skeletonema sp., Leptocylindrus sp., and Phaeocystis sp. Growth stages were initial, exponential, stationary, and degradation.
false
Ziervogel, K. (2018) Hydrolytic enzyme activities during CDOM monoculture experiments with Skeletonema, Leptocylindrus, and Phaeocystis. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-10-17 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.748423.1 [access date]
true
1
10.1575/1912/bco-dmo.748423.1
false
2018-10-17
Datapackage.json
Frictionless Data Package
https://www.bco-dmo.org/dataset/748423/datapackage.json
application/vnd.datapackage+json
PDF
https://www.bco-dmo.org/dataset/748423/Dataset_description.pdf
application/pdf
ISO 19115-2 (NOAA Profile)
https://www.bco-dmo.org/dataset/748423/iso
application/xml
http://www.isotc211.org/2005/gmd-noaa
Dublin Core
https://www.bco-dmo.org/dataset/748423/dublin-core
application/xml
http://purl.org/dc/elements/1.1/
748423
http://lod.bco-dmo.org/id/dataset/748423
2016-06-16 - 2016-08-27
2016-06-16
2016-06-16
---16
167
--06
2016
2016-08-27
2016-08-27
---27
239
--08
2016
VIEW TABLE
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_748423.htmlTable
text/html
SUBSET DATA
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_748423.html
text/html
CSV
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_748423.csv
text/csv
TSV
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_748423.tsv
text/tsv
MATLAB
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_748423.mat
text/x-matlab
netCDF
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_748423.nc
application/x-netcdf