http://lod.bco-dmo.org/id/dataset/748445 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2018-10-18 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Hydrolytic enzyme activities during CDOM monoculture experiment with Coscinodiscus 2018-10-17 publication 2018-10-17 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2019-04-01 publication https://doi.org/10.1575/1912/bco-dmo.748445.1 Kai Ziervogel University of New Hampshire principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Ziervogel, K. (2018) Hydrolytic enzyme activities during CDOM monoculture experiment with Coscinodiscus. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-10-17 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.748445.1 [access date] Bacterial cell counts during CDOM Coscinodiscus monoculture experiment Dataset Description: &lt;p&gt;This dataset is from a laboratory experiment. Four phytoplankton cultures and their associated bacterial communities were incubated in replicate roller bottles (1.9 L) over 3-6 weeks under laboratory conditions. Bacterial dynamics in the culture bottles were measured and correlated with geochemical parameters to determine the role of bacterial activities on the formation of CDOM in the cultures (Kinsey et al., 2018, see below).&lt;/p&gt; &lt;p&gt;The data include fluorescence and&amp;nbsp;bacterial enzyme activity during CDOM Coscinodiscus monoculture experiments.&amp;nbsp;Growth stages were initial and exponential.&lt;/p&gt; Methods and Sampling: &lt;p&gt;Hydrolytic enzyme activities were determined using L-leucine-4-methylcoumarinyl-7-amide (MCA) hydrochloride, 4-methylumbelliferyl α-D-glucopyranoside, and 4-methylumbelliferone (MUF) β-D-glucopyranoside (Sigma-Aldrich) as substrate proxies for leucine-aminopeptidase, α-glucosidase, and β-glucosidase activities, respectively. For each bottle and substrate proxy, 196 µL of unfiltered experimental or control water was added in duplicate to a pure-grade black 96-well plate (Brand Life Sciences) containing a single substrate proxy at saturation levels (final concentration 200 µM). Fluorescence (excitation 370 nm, emission 440 nm) was measured in a Tecan Infinite 200 Pro microplate reader immediately following the addition of the substrate and several more times over 7-20 h. The well plates were incubated in the dark at in situ temperature. MUF and MCA standard solutions prepared in seawater were used to determine hydrolysis rates. Killed controls (boiled sample water) and ultrapure water samples showed little change over the incubations.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459406 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459406 completed Kai Ziervogel University of New Hampshire 6038625478 kai.ziervogel@unh.edu pointOfContact asNeeded Dataset Version: 1 Unknown substrate sample fluor_t0 fluor_t1 fluor_t2 fluor_t3 time_t0 time_t1 time_t2 time_t3 enz_activity_t0 enz_activity_t1 enz_activity_t2 enz_activity_t3 SLOPE RSQR FACSCalibur flow cytometer (Becton-Dickson) Tecan Infinite 200 Pro microplate reader theme None, User defined No BCO-DMO term sample identification fluorescence time_elapsed featureType BCO-DMO Standard Parameters Flow Cytometer plate reader instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Planktonic Sources of Chromophoric Dissolved Organic Matter in Seawater https://osprey.bco-dmo.org/project/734581 Collaborative Research: Planktonic Sources of Chromophoric Dissolved Organic Matter in Seawater <p>NSF abstract:</p> <p>Chromophoric dissolved organic matter (CDOM) is a small but important fraction of the marine carbon pool that interacts with solar radiation and thus affects many photochemical and biological processes in the ocean. Despite its importance, the chemical basis for the formation of oceanic CDOM remains unclear. CDOM may be formed from two possible sources: 1) heterotrophic bacterial transformations of primary productivity (plankton-derived), or 2) terrestrially-derived. This project will examine the role of phytoplankton as a source of CDOM in the ocean by utilizing a powerful, new technique to measure particulate organic matter absorbance and fluorescence, discrete chemical measurements of probable precursors to planktonic CDOM, and enzymatic assays. Results of this research will provide new insights into the origin and production of planktonic CDOM and its transformation by heterotrophic bacteria. This research on CDOM will be shared broadly through a module at a North Carolina Aquarium, and streaming live feeds of shipboard activities to elementary school classrooms.</p> <p>Terrestrial and oceanic dissolved organic matter (DOM) differ in their chemical composition. Laboratory and open-ocean observations suggest that bacterial transformation of phytoplankton DOM produces humic-like CDOM signals that are visually similar to those in terrestrial CDOM. However, prior studies of oceanic CDOM using absorbance and fluorescence fit an electronic interaction (EI) model of intramolecular charge transfer (CT) reactions between donor and acceptor molecules common to partially-oxidized terrestrial molecules found in humic substances. This project will test the hypothesis that phytoplankton and bacteria provide a source of donors and acceptors that are microbially-transformed and linked, enabling CT contacts between them and creating oceanic CDOM. To address this, researchers will systematically study phytoplankton growth, including marine snow formation. A new technique for measuring base-extracted POM (BEPOM) absorbance and fluorescence will be used to incorporate planktonic CDOM results into the EI model, and supplemented with measurements of its probable chemical precursors. These experiments will improve understanding of how the production of CDOM in the ocean is linked to the optics and chemistry of planktonic CDOM formation. Determining the time course and extent of phytoplankton POM and DOM transformation by heterotrophic bacteria during the same phytoplankton growth experiments will provide an in-depth understanding as to how bacterial transformation of marine snow-associated planktonic organic matter drives CDOM production throughout the ocean.</p> PlankDOM largerWorkCitation project eng; USA biota oceans 2016-06-16 2016-08-27 Northern Atlantic Ocean, 34.65 N, 69.63 W 0 BCO-DMO catalogue of parameters from Hydrolytic enzyme activities during CDOM monoculture experiment with Coscinodiscus Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/748569.rdf Name: substrate Units: unitless Description: <p>substrate for measuring enzyme activity: a-glu = 4-methylumbelliferyl a-D-glucopyranoside; b-glu = 4-methylumbelliferone (MUF) ß-D-glucopyranoside; leu = L-leucine-4-methylcoumarinyl-7-amide</p> http://lod.bco-dmo.org/id/dataset-parameter/748570.rdf Name: sample Units: unitless Description: <p>sample identifier denoted as growth stage (days from start) replicate id</p> http://lod.bco-dmo.org/id/dataset-parameter/748571.rdf Name: fluor_t0 Units: relative fluorescence units Description: <p>fluorescence intensity at time 0</p> http://lod.bco-dmo.org/id/dataset-parameter/748572.rdf Name: fluor_t1 Units: relative fluorescence units Description: <p>fluorescence intensity at time 1</p> http://lod.bco-dmo.org/id/dataset-parameter/748573.rdf Name: fluor_t2 Units: relative fluorescence units Description: <p>fluorescence intensity at time 2</p> http://lod.bco-dmo.org/id/dataset-parameter/748574.rdf Name: fluor_t3 Units: relative fluorescence units Description: <p>fluorescence intensity at time 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748575.rdf Name: time_t0 Units: hours Description: <p>time since start of experiment; time point 0</p> http://lod.bco-dmo.org/id/dataset-parameter/748576.rdf Name: time_t1 Units: hours Description: <p>time elapsed from start of experiment; time point 1</p> http://lod.bco-dmo.org/id/dataset-parameter/748577.rdf Name: time_t2 Units: hours Description: <p>time elapsed from start of experiment; time point 2</p> http://lod.bco-dmo.org/id/dataset-parameter/748578.rdf Name: time_t3 Units: hours Description: <p>time elapsed from start of experiment; time point 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748579.rdf Name: enz_activity_t0 Units: nanoMol/hour Description: <p>enzymatic activity at time 0</p> http://lod.bco-dmo.org/id/dataset-parameter/748580.rdf Name: enz_activity_t1 Units: nanoMol/hour Description: <p>enzymatic activity at time 1</p> http://lod.bco-dmo.org/id/dataset-parameter/748581.rdf Name: enz_activity_t2 Units: nanoMol/hour Description: <p>enzymatic activity at time 2</p> http://lod.bco-dmo.org/id/dataset-parameter/748582.rdf Name: enz_activity_t3 Units: nanoMol/hour Description: <p>enzymatic activity at time 3</p> http://lod.bco-dmo.org/id/dataset-parameter/748583.rdf Name: SLOPE Units: unitless Description: <p>the slope of the graph of fluorescence intensity vs substrate concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/748584.rdf Name: RSQR Units: unitless Description: <p>the square of the correlation coefficient of fluorescence intensity vs substrate concentration</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 6646 https://darchive.mblwhoilibrary.org/bitstream/1912/23947/1/dataset-748445_hydrolytic-enzyme-activity-coscinodiscus__v1.tsv download https://doi.org/10.1575/1912/bco-dmo.748445.1 download onLine dataset &lt;p&gt;Hydrolytic enzyme activities were determined using L-leucine-4-methylcoumarinyl-7-amide (MCA) hydrochloride, 4-methylumbelliferyl α-D-glucopyranoside, and 4-methylumbelliferone (MUF) β-D-glucopyranoside (Sigma-Aldrich) as substrate proxies for leucine-aminopeptidase, α-glucosidase, and β-glucosidase activities, respectively. For each bottle and substrate proxy, 196 µL of unfiltered experimental or control water was added in duplicate to a pure-grade black 96-well plate (Brand Life Sciences) containing a single substrate proxy at saturation levels (final concentration 200 µM). Fluorescence (excitation 370 nm, emission 440 nm) was measured in a Tecan Infinite 200 Pro microplate reader immediately following the addition of the substrate and several more times over 7-20 h. The well plates were incubated in the dark at in situ temperature. MUF and MCA standard solutions prepared in seawater were used to determine hydrolysis rates. Killed controls (boiled sample water) and ultrapure water samples showed little change over the incubations.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;BCO-DMO Processing Notes:&lt;br /&gt; - added conventional header with dataset name, PI name, version date&lt;br /&gt; - modified parameter names to conform with BCO-DMO naming conventions&lt;br /&gt; - converted Excel file tables to a flat file and combined&amp;nbsp;a-glu, b-glu,&amp;nbsp;and leu substrate treatments.&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact FACSCalibur flow cytometer (Becton-Dickson) FACSCalibur flow cytometer (Becton-Dickson) PI Supplied Instrument Name: FACSCalibur flow cytometer (Becton-Dickson) PI Supplied Instrument Description:Used to make cell counts. Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ Tecan Infinite 200 Pro microplate reader Tecan Infinite 200 Pro microplate reader PI Supplied Instrument Name: Tecan Infinite 200 Pro microplate reader PI Supplied Instrument Description:Used to measure fluorescence from which hydrolysis rates were calculated. Instrument Name: plate reader Instrument Short Name: Instrument Description: Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.