http://lod.bco-dmo.org/id/dataset/748500
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-10-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Transparent exopolymer particle (TEP) measurements from the MesoHux mesocosm experiment held in May 2017
2018-09-14
publication
2018-09-14
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-05-20
publication
https://doi.org/10.26008/1912/bco-dmo.748500.1
Matthew D. Johnson
Woods Hole Oceanographic Institution
principalInvestigator
Kay D. Bidle
Rutgers University
principalInvestigator
Elizabeth Harvey
University of Georgia
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Johnson, M. D., Bidle, K. D., Harvey, E. (2021) Transparent exopolymer particle (TEP) measurements from the MesoHux mesocosm experiment held in May 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-09-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.748500.1 [access date]
Dataset Description: <p>Water samples (75-250 mL) were gently (&lt;150 mbar) filtered through 25 mm, 0.40 μm polycarbonate filters (Millipore, Isopore, HTTP02500). Post-filtration, filters were stained with 500 μL of a solution of 0.04% Alcian Blue (AB) and 0.06% acetic acid (pH 2.5) and were frozen at -20°C. For extraction, filters were immersed in sulfuric acid (80%) for 2 h and their absorbance was then measured spectrophotometrically (Agilent 8453 UV-visible spectrophotometer) at fixed wavelength of 787 nm. The AB-dye was calibrated using methods found in Bittar et al. (2018), where AB was used to stain known concentrations of xanthan gum (XG, Sigma-Aldrich). Two separate AB solutions (f factors 169 and 129) were used during the Bergen experiment. TEP&nbsp;concentrations (μg xanthan gum (Xg) equivalents L-1; μg XG eq. L-1) were calculated according to Passow and Alldredge (1995).</p>
<p><br />
Mesocosm treatments are as follows:<br />
P-limited: N:P added in a 60:1 ratio during the first 3 days of the experiment, no shading<br />
Redfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading<br />
Shaded: N:P added in a 16:1 ratio during the first 3 days of the experiment, top shaded of the mesocosm added on May 20, 2017<br />
Ambient: no nutrients added, no shading</p> Methods and Sampling:
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459190 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459190
completed
Matthew D. Johnson
Woods Hole Oceanographic Institution
508-289-2584
266 Woods Hole Rd. Watson Building 217, MS #52
Woods Hole
MA
02543
USA
mattjohnson@whoi.edu
pointOfContact
Kay D. Bidle
Rutgers University
848-932-3467
Department of Marine and Coastal Sciences 71 Dudley Road
New Brunswick
NJ
08901
USA
bidle@marine.rutgers.edu
pointOfContact
Elizabeth Harvey
University of Georgia
NH
USA
elizabeth.harvey@unh.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Exp_Day
Sample
Dilution
Treatment
Replication
Volume_Filtered
Absorbance
TEP
Aglient 8453 UV-visible spectrophotometer
theme
None, User defined
date
No BCO-DMO term
sample identification
dilution
treatment
replicate
volume of water filtered
absorbance
Transparent Exopolymer Particles
featureType
BCO-DMO Standard Parameters
Spectrophotometer
instrument
BCO-DMO Standard Instruments
MesoHux_2017
service
Deployment Activity
Raunefjord, 20 miles south of Bergen
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Quantifying competing loss rates of viral lysis and microzooplankton grazing on Emiliania huxleyi mortality
https://osprey.bco-dmo.org/project/645515
Quantifying competing loss rates of viral lysis and microzooplankton grazing on Emiliania huxleyi mortality
<p><em>Description from NSF award abstract:</em><br />
Processes that either promote growth or cause mortality drive the abundance of all organisms. For microbes such as phytoplankton, that have a lifespan measured in hours to days, small changes in these processes can have significant impacts. Phytoplankton are the central currency in the flow of material and nutrients throughout the marine environment. Even small shifts in their growth and mortality rates will have large-scale implications for ecosystem structure and biogeochemical cycling. While factors that influence growth are often examined, less is known regarding the regulation of phytoplankton mortality. This project will focus on quantifying competing modes of mortality on the bloom-forming coccolithophore, <em>Emiliania huxleyi,</em> a globally important phytoplankton species that contributes significantly to ocean carbon and sulfur cycles. Mortality due to grazing by single-celled microzooplankton is the largest contributor to phytoplankton loss in the marine environment. However, <em>E. </em><em>huxleyi</em> also has a well-characterized relationship with a virus that can result in mass mortality. Therefore, <em>E. </em><em>huxleyi</em><em> </em>serves as a good model organism for examining how mortality is partitioned between grazing by microzooplankton predators and lysis due to viral infection. Quantifying these mortality mechanisms will help to inform mathematical models for the accurate prediction of shifts in <em>E. </em><em>huxleyi</em><em> </em>population dynamics and ultimately, primary production and biogeochemical cycling. This work will involve collaboration with a high school science teacher in a school system with a large proportion of students from underrepresented groups, in the creation and implementation of short film clips that depict important ecological interactions. These film clips will then be incorporated into laboratory activities to communicate these concepts to students. Further, undergraduate students from underrepresented groups will be trained at both Woods Hole Oceanographic Institute and Rutgers University, to perform laboratory research on mortality processes on phytoplankton. This research will also provide training and career development for a postdoctoral scientist.</p>
<p>Mortality mechanisms in phytoplankton have generally been studied independent from one another, however in nature, these processes act concurrently. The relative proportion that microzooplankton grazing and viral lysis contribute to overall <em>E. </em><em>huxleyi</em> loss and how they may interact to shape bloom dynamics is largely unknown. Understanding the relative importance of these processes, as well as their interaction, is critical due to their contrasting influence on the structure and function of marine food webs and biogeochemical cycles. While grazing tends to channel phytoplankton biomass to higher trophic levels, viral lysis stimulates microbial loop activity and vertical particle export flux. This research will determine the effect of one mortality process on the other, as well as their net effect on<em> E. </em><em>huxleyi</em><em> </em>population dynamics and export in both laboratory and field mesocosm experiments. This integrated approach will provide a unique mechanistic perspective of multi-trophic microbial interactions, thereby increasing the potential for accurate predictions of <em>E. </em><em>huxleyi</em> population dynamics and biogeochemical cycling. The outcomes of this research have the potential to yield broadly applicable insights into how microbial interactions can drive ecological and biogeochemical dynamics in the marine environment.</p>
<p><strong>This project is funded by an NSF Collaborative Research award.</strong></p>
E huxleyi Mortality
largerWorkCitation
project
eng; USA
oceans
Raunefjord, 20 miles south of Bergen
2017-05-14
2017-05-30
0
BCO-DMO catalogue of parameters from Transparent exopolymer particle (TEP) measurements from the MesoHux mesocosm experiment held in May 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/748517.rdf
Name: Date
Units: unitless
Description: <p>date the sample was taken in YYYY-MM-DD format</p>
http://lod.bco-dmo.org/id/dataset-parameter/748518.rdf
Name: Exp_Day
Units: unitless
Description: <p>day of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/748519.rdf
Name: Sample
Units: unitless
Description: <p>sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/748520.rdf
Name: Dilution
Units: unitless
Description: <p>dilution percent</p>
http://lod.bco-dmo.org/id/dataset-parameter/748521.rdf
Name: Treatment
Units: unitless
Description: <p>type of treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/748522.rdf
Name: Replication
Units: unitless
Description: <p>replicate identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/748523.rdf
Name: Volume_Filtered
Units: mililiters (mL)
Description: <p>volume filtered</p>
http://lod.bco-dmo.org/id/dataset-parameter/748524.rdf
Name: Absorbance
Units: nanometers (nm)
Description: <p>absorbance</p>
http://lod.bco-dmo.org/id/dataset-parameter/748525.rdf
Name: TEP
Units: microgram TEP equivalent
Description: <p>calibrated transparent exopolymer particle (TEP)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6103
https://darchive.mblwhoilibrary.org/bitstream/1912/27140/1/dataset-748500_mesohux-tep__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.748500.1
download
onLine
dataset
<p>BCO-DMO Processing Notes:</p>
<ul>
<li>converted date from Mon, DD, YYYY to YYYY-MM-DD</li>
<li>replace spaces with underscores in the field names</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Aglient 8453 UV-visible spectrophotometer
Aglient 8453 UV-visible spectrophotometer
PI Supplied Instrument Name: Aglient 8453 UV-visible spectrophotometer PI Supplied Instrument Description:For extraction, filters were immersed in sulfuric acid (80%) for 2 h and their absorbance was then measured spectrophotometrically (Agilent 8453 UV-visible spectrophotometer) at fixed wavelength of 787 nm. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Deployment: MesoHux_2017
MesoHux_2017
National Mesocosm Centre
National Mesocosm Centre
National Mesocosm Centre
National Mesocosm Centre