http://lod.bco-dmo.org/id/dataset/748792
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-10-25
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Porewater measurements of nitrate and nitrite concentration and N and O isotopic ratios (d15N and d18O) collected from sites 3 and 10 on the North Atlantic Long Core Cruise R/V Knorr KN223 from October to December 2014
2018-10-26
publication
2018-10-26
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-15
publication
https://doi.org/10.1575/1912/bco-dmo.748792.1
Carolyn Buchwald
Woods Hole Oceanographic Institution
principalInvestigator
Arthur J. Spivack
University of Rhode Island
principalInvestigator
Scott Wankel
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Buchwald, C., Spivack, A., Wankel, S. (2018) Porewater measurements of nitrate and nitrite concentration and N and O isotopic ratios (d15N and d18O) collected from sites 3 and 10 on the North Atlantic Long Core Cruise R/V Knorr KN223 from October to December 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-10-26 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.748792.1 [access date]
Porewater measurements of nitrate and nitrite concentration and N and O isotopic ratios (d15N and d18O) from sites 3 and 10 on the North Atlantic Long Core Cruise R/V Knorr KN223 Dataset Description: <p>Porewater measurements of nitrate and nitrite concentration and N and O isotopic ratios (d15N and d18O) from sites 3 and 10 on the North Atlantic Long Core Cruise R/V Knorr KN223.</p> Methods and Sampling: <p>Samples were collected aboard the R/V Knorr using its long coring system in November of 2014 on cruise KN-223 in the North Atlantic. Samples used in this study came from two sediment coring sites located within 90m of each other at 50°37.25'W, 14°24.05'N, and 4455 m water depth. Porewaters were extracted at approximately 0.5m intervals from two long piston cores (30 and 34 m long) using Rhizon samplers (0.2 mm pore size) and either analyzed shipboard or frozen until analyses were conducted shore side.</p>
<p>Nitrate and nitrite concentrations were determined shipboard using ion chromatography with UV detection (D'Hondt et al., 2015). Isotopes were measured in the Wankel lab (Woods Hole Oceanographic Institution) using an Isoprime 100 isotope ratio mass spectrometer coupled to a modified TraceGas prep system similar to that described previously (McIlvin and Casciotti, 2011), which is used to flush, purify and cryogenically trap sample N<sub>2</sub>O from converted nitrate or nitrite samples. Nitrate isotopic composition was measured using the denitrifier method to convert nitrate to N<sub>2</sub>O, normalized to international reference materials (USGS 34, USGS 32, and USGS 35) (Sigman et al., 2001; Casciotti et al., 2002). Nitrite isotope measurements were made separately using the azide method for conversion of nitrite to N<sub>2</sub>O (McIlvin and Altabet, 2005), normalizing to previously calibrated Wankel isotope lab standards (WILIS 10, 11, and 20) (Buchwald et al., 2016). Where co-occurring nitrite concentrations were less than 5 times as high as nitrate, nitrite was removed by addition of sulfamic acid (Granger and Sigman, 2009) prior to the denitrifier method. In the deepest samples having measurable nitrate, where concentrations were very low, the N and O isotopic composition of nitrate was calculated by mass balance using analyses of the combined nitrate + nitrite pools by the denitrifier method, in which both nitrate and nitrite standards were also analyzed, together with nitrite isotope values from the azide-only measurements described previously (Casciotti and McIlvin, 2007).&nbsp; &nbsp;&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0939564 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0939564
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1433150 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1433150
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1537485 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1537485
completed
Carolyn Buchwald
Woods Hole Oceanographic Institution
978-302-5481
1355 Oxford Street
Halifax
NS
B3H 1Z1
Canada
cbuchwald@dal.ca
pointOfContact
Arthur J. Spivack
University of Rhode Island
401-874-6200
215 South Ferry Road
Narragansett
RI
02882
USA
spivack@uri.edu
pointOfContact
Scott Wankel
Woods Hole Oceanographic Institution
508-289-3944
266 Woods Hole Rd. MS #25
Woods Hole
MA
02543
USA
sdwankel@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Site_Number
Latitude
Longitude
Depth_for_O2
Oxygen
Depth_for_Nitrate_and_Nitrate
Nitrate
Nitrite
Depth_for_Nitrite_isotopes
d15N_Nitrite
d18O_Nitrite
Depth_for_Nitrate_isotopes
d15N_Nitrate
d18O_Nitrate
Depth_for_Ammonium
Ammonium
ion chromatography with UV detection
Isoprime 100 isotope ratio mass spectrometer
R/V Knorr long coring system
Rhizon samplers
theme
None, User defined
site
latitude
longitude
depth below seafloor
dissolved Oxygen
Nitrate
Nitrite
d15N_NO3
d18O_NO3
Ammonium
featureType
BCO-DMO Standard Parameters
Ion Chromatograph
Isotope-ratio Mass Spectrometer
Piston Corer
Sediment Porewater Sampler
instrument
BCO-DMO Standard Instruments
KN223
service
Deployment Activity
North and West Atlantic Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Center for Dark Energy Biosphere Investigations
http://www.darkenergybiosphere.org
Center for Dark Energy Biosphere Investigations
The mission of the Center for Dark Energy Biosphere Investigations (C-DEBI) is to explore life beneath the seafloor and make transformative discoveries that advance science, benefit society, and inspire people of all ages and origins.
C-DEBI provides a framework for a large, multi-disciplinary group of scientists to pursue fundamental questions about life deep in the sub-surface environment of Earth. The fundamental science questions of C-DEBI involve exploration and discovery, uncovering the processes that constrain the sub-surface biosphere below the oceans, and implications to the Earth system. What type of life exists in this deep biosphere, how much, and how is it distributed and dispersed? What are the physical-chemical conditions that promote or limit life? What are the important oxidation-reduction processes and are they unique or important to humankind? How does this biosphere influence global energy and material cycles, particularly the carbon cycle? Finally, can we discern how such life evolved in geological settings beneath the ocean floor, and how this might relate to ideas about the origin of life on our planet?
C-DEBI's scientific goals are pursued with a combination of approaches:
(1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites;
(2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems;
(3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and
(4) entrain, educate, inspire, and mentor an interdisciplinary community of researchers and educators, with an emphasis on undergraduate and graduate students and early-career scientists.
Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI.
Data Management:
C-DEBI is committed to ensuring all the data generated are publically available and deposited in a data repository for long-term storage as stated in their Data Management Plan (PDF) and in compliance with the NSF Ocean Sciences Sample and Data Policy. The data types and products resulting from C-DEBI-supported research include a wide variety of geophysical, geological, geochemical, and biological information, in addition to education and outreach materials, technical documents, and samples. All data and information generated by C-DEBI-supported research projects are required to be made publically available either following publication of research results or within two (2) years of data generation.
To ensure preservation and dissemination of the diverse data-types generated, C-DEBI researchers are working with BCO-DMO Data Managers make data publicly available online. The partnership with BCO-DMO helps ensure that the C-DEBI data are discoverable and available for reuse. Some C-DEBI data is better served by specialized repositories (NCBI's GenBank for sequence data, for example) and, in those cases, BCO-DMO provides dataset documentation (metadata) that includes links to those external repositories.
C-DEBI
largerWorkCitation
program
Determining the rates of denitrification, nitrification, and nitrogen fixation using natural abundance isotope profiles in North Atlantic sediments
https://www.darkenergybiosphere.org/award/determining-the-rates-of-denitrification-nitrification-and-nitrogen-fixation-using-natural-abundance-isotope-profiles-in-north-pond-sediments/
Determining the rates of denitrification, nitrification, and nitrogen fixation using natural abundance isotope profiles in North Atlantic sediments
<p>Project Abstract:<br />
Deep-sea sediments in the oligotrophic ocean host a diverse array of microbes that are involved in multiple processes within the nitrogen cycle. Using measurements of nitrate and nitrite, and their stable isotopes (d15N and d18O) in sedimentary pore fluids, we have been developing approaches for determining the distribution and magnitude of key processes in the oligotrophic sediments of the North Atlantic. While concentration profiles alone indicate the production of nitrate through nitrification in the surface sediments and the reduction of the nitrate deeper in the absence of oxygen, the dual stable isotope profiles of NO3- and NO2- demonstrate clear evidence of further complexity; specifically, that nitrite oxidation occurs deeper in the sediments as well, apparently in the absence of O2. A number of lines of evidence contribute to this refined understanding of the distribution of N cycling processes in these environments, including large differences in the nitrate and nitrite d15N, as well as the evolution of a greater than 1:1 relationship between the d15N and d18O of nitrate. We used a 1D inverse model that predicts the distribution and rates of different oxidative and reductive nitrogen cycling processes throughout these vertical profiles. Our analysis reveals that nitrate reduction and nitrite oxidation co-occur between 0 and 10 meters, and that the ratio of these processes changes in relation to the abundance of porewater oxygen. In the upper profile where dissolved oxygen is more abundant oxidative processes (e.g., nitrite re-oxidation) play an exceptionally large role, as reflected in the very high slope for the evolving relationship between d15N and d18O nitrate. Below the depth of oxygen penetration, while nitrate reduction becomes a substantially more important processes, a clear indication of oxidation remains – as reflected in the large difference between nitrate and nitrite d15N. All rates were predicted to be slow on the order of 0.1 mM per year, which was substantiated by d18O values of nitrite reflecting complete isotopic equilibration with water.</p>
<p>This project was funded by a <a href="https://www.darkenergybiosphere.org/award/determining-the-rates-of-denitrification-nitrification-and-nitrogen-fixation-using-natural-abundance-isotope-profiles-in-north-pond-sediments/" target="_blank">C-DEBI Postdoctoral Fellowship</a> to Carolyn Buchwald (advisor: Scott Wankel).</p>
North Atlantic Nitrate and Nitrite
largerWorkCitation
project
eng; USA
oceans
North and West Atlantic Ocean
-50.6207
-50.6203
14.4
14.4007
2014-10-25
2014-12-02
North Pond, North Atlantic
0
BCO-DMO catalogue of parameters from Porewater measurements of nitrate and nitrite concentration and N and O isotopic ratios (d15N and d18O) collected from sites 3 and 10 on the North Atlantic Long Core Cruise R/V Knorr KN223 from October to December 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/748814.rdf
Name: Site_Number
Units: unitless
Description: <p>Site number where samples were collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/748815.rdf
Name: Latitude
Units: decimal degress
Description: <p>Latitude of site; North = positive values</p>
http://lod.bco-dmo.org/id/dataset-parameter/748816.rdf
Name: Longitude
Units: decimal degress
Description: <p>Longitude of site; East = positive values</p>
http://lod.bco-dmo.org/id/dataset-parameter/748817.rdf
Name: Depth_for_O2
Units: meters below seafloor (mbsf)
Description: <p>Depth of sample for oxygen (O2) measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/748818.rdf
Name: Oxygen
Units: micromolar (uM)
Description: <p>Oxygen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/748819.rdf
Name: Depth_for_Nitrate_and_Nitrate
Units: meters below seafloor (mbsf)
Description: <p>Depth of sample for nitrate and nitrite measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/748820.rdf
Name: Nitrate
Units: micromolar (uM)
Description: <p>Nitrate concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/748821.rdf
Name: Nitrite
Units: micromolar (uM)
Description: <p>Nitrite concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/748822.rdf
Name: Depth_for_Nitrite_isotopes
Units: meters below seafloor (mbsf)
Description: <p>Depth of sample for nitrite isotope measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/748823.rdf
Name: d15N_Nitrite
Units: per mil (‰)
Description: <p>d15N nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/748824.rdf
Name: d18O_Nitrite
Units: per mil (‰)
Description: <p>d18O nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/748825.rdf
Name: Depth_for_Nitrate_isotopes
Units: meters below seafloor (mbsf)
Description: <p>Depth of sample for nitrate isotope measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/748826.rdf
Name: d15N_Nitrate
Units: per mil (‰)
Description: <p>d15N nitrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/748827.rdf
Name: d18O_Nitrate
Units: per mil (‰)
Description: <p>d18O nitrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/748828.rdf
Name: Depth_for_Ammonium
Units: meters below seafloor (mbsf)
Description: <p>Depth of sample for ammonium measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/748829.rdf
Name: Ammonium
Units: micromolar (uM)
Description: <p>Ammonium concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
14431
https://darchive.mblwhoilibrary.org/bitstream/1912/23815/1/dataset-748792_porewater-nitrogen__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.748792.1
download
onLine
dataset
<p>Samples were collected aboard the R/V Knorr using its long coring system in November of 2014 on cruise KN-223 in the North Atlantic. Samples used in this study came from two sediment coring sites located within 90m of each other at 50°37.25'W, 14°24.05'N, and 4455 m water depth. Porewaters were extracted at approximately 0.5m intervals from two long piston cores (30 and 34 m long) using Rhizon samplers (0.2 mm pore size) and either analyzed shipboard or frozen until analyses were conducted shore side.</p>
<p>Nitrate and nitrite concentrations were determined shipboard using ion chromatography with UV detection (D'Hondt et al., 2015). Isotopes were measured in the Wankel lab (Woods Hole Oceanographic Institution) using an Isoprime 100 isotope ratio mass spectrometer coupled to a modified TraceGas prep system similar to that described previously (McIlvin and Casciotti, 2011), which is used to flush, purify and cryogenically trap sample N<sub>2</sub>O from converted nitrate or nitrite samples. Nitrate isotopic composition was measured using the denitrifier method to convert nitrate to N<sub>2</sub>O, normalized to international reference materials (USGS 34, USGS 32, and USGS 35) (Sigman et al., 2001; Casciotti et al., 2002). Nitrite isotope measurements were made separately using the azide method for conversion of nitrite to N<sub>2</sub>O (McIlvin and Altabet, 2005), normalizing to previously calibrated Wankel isotope lab standards (WILIS 10, 11, and 20) (Buchwald et al., 2016). Where co-occurring nitrite concentrations were less than 5 times as high as nitrate, nitrite was removed by addition of sulfamic acid (Granger and Sigman, 2009) prior to the denitrifier method. In the deepest samples having measurable nitrate, where concentrations were very low, the N and O isotopic composition of nitrate was calculated by mass balance using analyses of the combined nitrate + nitrite pools by the denitrifier method, in which both nitrate and nitrite standards were also analyzed, together with nitrite isotope values from the azide-only measurements described previously (Casciotti and McIlvin, 2007).&nbsp; &nbsp;&nbsp;</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
-&nbsp;modified parameter names to conform to BCO-DMO naming conventions (removed parentheses);<br />
- converted original lat/lon values (degrees &amp; decimal mins) to decimal degrees;<br />
- replaced blank cells&nbsp;(no data) with "nd";<br />
- combined data from both sites into one dataset.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
ion chromatography with UV detection
ion chromatography with UV detection
PI Supplied Instrument Name: ion chromatography with UV detection Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)
Isoprime 100 isotope ratio mass spectrometer
Isoprime 100 isotope ratio mass spectrometer
PI Supplied Instrument Name: Isoprime 100 isotope ratio mass spectrometer PI Supplied Instrument Description:Isoprime 100 isotope ratio mass spectrometer coupled to a modified TraceGas prep system Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
R/V Knorr long coring system
R/V Knorr long coring system
PI Supplied Instrument Name: R/V Knorr long coring system Instrument Name: Piston Corer Instrument Short Name:Piston Corer Instrument Description: The piston corer is a type of bottom sediment sampling device. A long, heavy tube is plunged into the seafloor to extract samples of mud sediment. A piston corer uses a "free fall" of the coring rig to achieve a greater initial force on impact than gravity coring. A sliding piston inside the core barrel reduces inside wall friction with the sediment and helps to evacuate displaced water from the top of the corer. A piston corer is capable of extracting core samples up to 90 feet in length. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/51/
Rhizon samplers
Rhizon samplers
PI Supplied Instrument Name: Rhizon samplers PI Supplied Instrument Description:Rhizon samplers extract small volumes of pore water from soil and sediments. See: https://www.rhizosphere.com/ Instrument Name: Sediment Porewater Sampler Instrument Short Name: Instrument Description: A device that collects samples of pore water from various horizons below the seabed.
Cruise: KN223
KN223
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel
KN223
Richard W Murray
Boston University
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel