http://lod.bco-dmo.org/id/dataset/748866
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-10-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Videos of coscinodiscus sinking behavior in light and dark conditions from experiments in June 2017
2018-10-26
publication
2018-10-26
revision
BCO-DMO Linked Data URI
2018-10-26
creation
http://lod.bco-dmo.org/id/dataset/748866
Brad J. Gemmell
University of South Florida
principalInvestigator
Tracy A. Villareal
University of Texas at Austin
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gemmell, B. J., Villareal, T. A. (2018) Videos of coscinodiscus sinking behavior in light and dark conditions from experiments in June 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-10-26 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/748866 [access date]
Videos of coscinodiscus sinking behavior. Dataset Description: <p>Videos of Coscinodiscus wailesii and Coscinodiscus radiatus diatoms sinking following&nbsp;exposure to various culture and light conditions.</p>
<p>Each date folder contains folders with videos from that date and a scale video.<br />
Videos were recorded at 10 frames per second.</p>
<p>Within each date folder are folders for each treatment. Folder names describe treatment conditions. Media refers to L1 culture media. Light and dark refer to 12 h exposure to light or dark prior to video recording.</p>
<p>Treatments:<br />
6_20_2017 (L1 culture media):<br />
L1 lg C_wailesii_exponential phase<br />
L1 lg C_radiatus_exponential phase</p>
<p>6_21_2017:<br />
C_radiatus Dark_FSW for 24 hrs_from 9 day old media<br />
C_radiatus Dark_L1 for 24 hrs_9 day old media<br />
C_radiatus Light_FSW for 24 hrs_from 9 day old media<br />
C_radiatus Light_L1 for 24 hrs_from 9 day old media<br />
C_radiatus Light_L1 for 24 hrs_from diff stock_very sm cells<br />
C_wailesii_lg_Dark-L1 for 24 hrs<br />
C_wailesii_lg_Dark_FSW for 24 hrs<br />
C_wailesii_lg_light_FSW for 24 hrs</p>
<p>6_22_2017:<br />
C_radiatus_Dark_FSW 48 hrs<br />
C_radiatus_Dark_L1 48 hrs_9 day old media<br />
C_radiatus_Light_FSW 48 hrs<br />
C_radiatus_Light_L1 48 hrs_recently transferred to new L1<br />
C_wailesii_lg_Dark_FSW 48 hrs<br />
C_wailesii_lg_Dark_L1 48 hrs<br />
C_wailesii_lg_Light_FSW 48 hrs</p>
<p>NOTE:&nbsp;The Get Data link will download a 3.2 GB .zip data package with all the video files.</p> Methods and Sampling: <p>Cultures of two diatom species Coscinodiscus wailesii and Cosciondiscus radiatus were filmed in L1 culture media or after transferring to filtered seawater for 24 or 48 h and exposing to either light or dark conditions for 12 h. Methods are described in more detail in Du Clos et al. in prep.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1537546 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1537546
completed
Brad J. Gemmell
University of South Florida
813-974-1228
Department of Integrative Biology 4202 E. Fowler Ave
Tampa
FL
33620
USA
bgemmell@usf.edu
pointOfContact
Tracy A. Villareal
University of Texas at Austin
361-749-6732
Marine Science Institute and Dept. of Marine Science 750 Channel View Dr.
Port Aransas
TX
78373
USA
tracyv@austin.utexas.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Edgertronic SC1 camera
theme
None, User defined
Camera
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dynamic sinking behavior in diatoms: New insights from individual-based high resolution video observations
https://osprey.bco-dmo.org/project/651493
Dynamic sinking behavior in diatoms: New insights from individual-based high resolution video observations
<p><em>Description from NSF award abstract:</em><br />
The sinking of diatoms out of the well-lit upper layers of the ocean is responsible for transport of material to the deep-sea and is an important factor in controlling the overall abundance of this grass of the sea. Their sinking characteristics are important to understand in detail so they can be accurately represented in models of ocean dynamics. It has been assumed that all members of these non-flagellated, microscopic cells sink at approximately the same rate, at a constant rate, and that the direction of motion is downward. However, a re-examination of sinking rates at an individual cell level indicates that all three assumptions are incorrect. Using sophisticated optical and computing techniques, these researchers are examining how individual diatom cells sink, their ability to start and stop, and assessing what fraction can actually ascend. This study will yield new insights into how diatoms interact with their external environment by altering their movement through it. It will also address what fraction of these populations are actually moving upwards, thereby enhancing the movement of nutrients upward into the well-lit portions of the ocean. These are novel insights into how small unicellular species interact with the ocean around them and will significantly enrich our understanding of a problem that had been thought to be well understood. The project will train one graduate student and two undergraduate students in this research. Outreach is also provided by K-12 activities bringing holographic instruments into the classroom, and a public lecture series at our institute.</p>
<p>Diatom sinking rates are important life history characteristics that control both loss rates and nutrient flux to the cell surface. Positive buoyancy (m per hour rates) is an attribute of the largest diatom cells and plays a role in a vertical migration life history strategy. However, rates in smaller diatoms are typically described from a modified Stokes equation and are generally assumed to uniform and downward. The investigators previously observed that a species sinking rate is not monotonic within a sample but is distributed around a mean value, may be both upward and downward, and is under cellular control from near-zero to maximum velocity over second time scales. Thus, ascending behavior can be limited to a small portion of a population with a substantial downward rate. The goal of this project is to determine how widespread these characteristics are, determine the role of this unique start-stop sinking behavior, and examine how pervasive positive buoyancy is using a series of carefully controlled laboratory studies and a broad suite of diatom species. These characteristics will be considered within a framework of the complex form/function patterns that occur in diatoms. Boundary layers around cells differ vastly during the stop/start sequence and can be directly visualized by our techniques. Nutrient diffusion to the cell is accelerated during fast sinking; the investigators hypothesize that diffusion to cellular surfaces has been underestimated by using a constant bulk sinking rate. This work is only possible with the advent of high resolution cameras and advanced processing that allows particle and fluid flow to be quantified in a dynamic water column.</p>
Diatom Dynamic Sinking
largerWorkCitation
project
eng; USA
oceans
2017-06-20
2017-06-22
laboratory studies, Univ. of Texas at Austin, University of South Florida
0
BCO-DMO catalogue of parameters from Videos of coscinodiscus sinking behavior in light and dark conditions from experiments in June 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3432864665
https://datadocs.bco-dmo.org/file/P6qQ11QH3Jg5Zx/diatom_videos.zip
diatom_videos.zip
download
https://osprey.bco-dmo.org/dataset/748866/data/download
download
onLine
dataset
<p>Cultures of two diatom species Coscinodiscus wailesii and Cosciondiscus radiatus were filmed in L1 culture media or after transferring to filtered seawater for 24 or 48 h and exposing to either light or dark conditions for 12 h. Methods are described in more detail in Du Clos et al. in prep.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing Notes:</p>
<ul>
<li>Folders 6_20_2017, 6_21_2017,&nbsp;and 6_22_2017&nbsp;have been zipped into the package diatom_videos.zip and served via the "Get Data" button.</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Edgertronic SC1 camera
Edgertronic SC1 camera
PI Supplied Instrument Name: Edgertronic SC1 camera PI Supplied Instrument Description:Camera: Edgertronic SC1 camera (Sanstreak Corp., San Jose, CA, USA)
Lens: Nikon 105 mm 1:1 macro lens
Resolution: 1280 x 1024 px
Frame rate: 10 fps
Illumination: LED infrared illuminator Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/