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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/748930.rdf" xlink:actuate="onRequest">Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) - growth under bubbling stress: A-pulse raw fluorescence data</gmx:Anchor>
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        <gco:CharacterString>Series 3B: A-pulse raw fluorescence Dataset Description: &amp;lt;p&amp;gt;Experiments were conducted to determine the optimal actinic light pulse (A-pulse) setting for NPQ1 protocols of the AquaPen for assessment of non-photochemical quenching in Thalassiosira pseudonana CCMP 1014 cultures grown at different light environments.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Experiments were conducted in the lab at the University of California Santa Barbara&amp;amp;nbsp;to determine the optimal actinic light pulse (A-pulse) setting for NPQ1 protocols of the AquaPen for assessment of non-photochemical quenching in Thalassiosira pseudonana CCMP 1014 cultures grown at different light environments. TP1014 stock cultures were maintained in artificial sea water (Kester et. al 1967), enriched as with f/2 media (Guillard 1975). For the experiment, 5 ml of the TP1014 stock cultures was inoculated into 75 ml of ASW in four tubes. The tubes were incubated in a Multicultivator MC-1000 OD unit (Qubit Systems), at 25°C and light intensities of 200, 400, 600 and 800 µmol photons·m-2·sec-1 respectively, set at a 12:12 day:night cycle for four days. All samples were bubbled with air at 60 ml·min-1 through a 0.2 µm stainless steel carbonating stones. Samples were collected from each tube after three days and analyzed used for assessment of photochemistry using the Aquapen-C AP-C 100 (Photon Systems Instruments). Three ml samples were placed in the dark at 25°C for a minimum of 30 minutes prior to measuring photochemistry. The NPQ1 protocol on the instrument was used for assessment of non-photochemical quenching in samples. The NPQ1 protocol administers 5 light pulses over 60 seconds during actinic light exposure, followed by 3 light pulses over 88 seconds during recovery in the dark. Blue light (455 nm) was used as actinic light in these experiments. Baseline measurements were made at f-pulse settings of 0.03 μmol · m-2 · s-1, Saturating pulses were set at 2100 μmol · m-2 · s-1, and actinic light pulses (for the NPQ1 protocol only) were set at 100, 200, 300, 400, 500, 600, 700, and 800 µmol photons·m-2·sec-1 respectively for each sample.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/654346.rdf" xlink:title="OCE-1538602" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538602 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538602</gmx:Anchor>
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	Name: flr_T200_AP300
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 300 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772067.rdf
	Name: flr_T200_AP400
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 400 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772068.rdf
	Name: flr_T200_AP500
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 500 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772069.rdf
	Name: flr_T200_AP600
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 600 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772070.rdf
	Name: flr_T200_AP700
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 700 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772071.rdf
	Name: flr_T200_AP800
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 200 micromole photons/meter^2/second and a pulse setting at 800 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772072.rdf
	Name: flr_T400_AP100
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 100 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772073.rdf
	Name: flr_T400_AP200
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 200 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772074.rdf
	Name: flr_T400_AP300
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 300 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772075.rdf
	Name: flr_T400_AP400
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 400 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772076.rdf
	Name: flr_T400_AP500
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 500 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772077.rdf
	Name: flr_T400_AP600
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 600 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772078.rdf
	Name: flr_T400_AP700
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 700 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772079.rdf
	Name: flr_T400_AP800
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 400 micromole photons/meter^2/second and a pulse setting at 800 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772080.rdf
	Name: flr_T600_AP100
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 100 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772081.rdf
	Name: flr_T600_AP200
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 200 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772082.rdf
	Name: flr_T600_AP300
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 300 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772083.rdf
	Name: flr_T600_AP400
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 400 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772084.rdf
	Name: flr_T600_AP500
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 500 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772085.rdf
	Name: flr_T600_AP600
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 600 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772086.rdf
	Name: flr_T600_AP700
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 700 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772087.rdf
	Name: flr_T600_AP800
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 600 micromole photons/meter^2/second and a pulse setting at 800 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772088.rdf
	Name: flr_T800_AP100
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 100 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772089.rdf
	Name: flr_T800_AP200
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 200 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772090.rdf
	Name: flr_T800_AP300
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 300 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772091.rdf
	Name: flr_T800_AP400
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 400 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772092.rdf
	Name: flr_T800_AP500
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 500 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772093.rdf
	Name: flr_T800_AP600
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 600 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772094.rdf
	Name: flr_T800_AP700
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 700 micromol photons/meter^2/second&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/772095.rdf
	Name: flr_T800_AP800
	Units: Relative fluorescence units (RFU)
	Description: &lt;p&gt;fluorescence measurements of T.pseudonana CCMP 1014 cultures grown at 25°C and 800 micromole photons/meter^2/second and a pulse setting at 800 micromol photons/meter^2/second&lt;/p&gt; 
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            <gco:CharacterString>PI Supplied Instrument Name: Multicultivator MC-1000 OD (Qubit Systems) PI Supplied Instrument Description:Used for incubation of TP1014 cultures. Instrument Name: Cell Cultivator Instrument Short Name:   Instrument Description: An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/484.rdf" xlink:title="Fluorometer" xlink:actuate="onRequest">Aquapen-C AP-C 100 (Photon Systems Instruments)</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Aquapen-C AP-C 100 (Photon Systems Instruments)</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Aquapen-C AP-C 100 (Photon Systems Instruments) PI Supplied Instrument Description:Used to measure fluorescence.
A hand-held cuvette version of the FluorPen fluorometer equipped with a blue and red LED emitter. Blue excitation light (455 nm) is intended for chlorophyll excitation, i.e., for measuring chlorophyll fluorescence in algal cultures. Red-orange excitation light (620 nm) is intended for excitation through phycobilins and is suitable for measuring in cyanobacteria. Instrument Name: Fluorometer Instrument Short Name:Fluorometer   Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
