http://lod.bco-dmo.org/id/dataset/748978
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
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2018-10-29
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Series 3B: Supplemental experiments on Thalassiosira pseudonana (CCMP1014) cultures determining the optimal aquapen actinic light pulse (A-pulse) setting: computed values including min and max fluorescence
2018-10-29
publication
2018-10-29
revision
BCO-DMO Linked Data URI
2018-10-29
creation
http://lod.bco-dmo.org/id/dataset/748978
Uta Passow
University of California-Santa Barbara
principalInvestigator
Edward Laws
Louisiana State University
principalInvestigator
Nigel D'Souza
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Passow, U., Laws, E., D'Souza, N. (2018) Series 3B: Supplemental experiments on Thalassiosira pseudonana (CCMP1014) cultures determining the optimal aquapen actinic light pulse (A-pulse) setting: computed values including min and max fluorescence. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-10-29 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/748978 [access date]
Series 3B: Supplemental experiments on T. pseudonana (CCMP1014): raw fluorescence Dataset Description: <p>Experiments were conducted to determine the optimal actinic light pulse (A-pulse) setting for NPQ1 protocols of the AquaPen for assessment of non-photochemical quenching in Thalassiosira pseudonana CCMP 1014 cultures grown at different light environments.</p>
<p>Computed values include min and max fluorescence in dark-adapted state, quantum yield of the Photosystem II, non-photochemical chlorophyll fluorescence quenching and the coefficient of photochemical quenching (an estimate of open PSII reaction centers during these corresponding measurements).</p> Methods and Sampling: <p>Experiments were conducted in the lab at the University of California Santa Barbara&nbsp;to determine the optimal actinic light pulse (A-pulse) setting for NPQ1 protocols of the AquaPen for assessment of non-photochemical quenching in Thalassiosira pseudonana CCMP 1014 cultures grown at different light environments. TP1014 stock cultures were maintained in artificial sea water (Kester et. al 1967), enriched as with f/2 media (Guillard 1975). For the experiment, 5 ml of the TP1014 stock cultures was inoculated into 75 ml of ASW in four tubes. The tubes were incubated in a Multicultivator MC-1000 OD unit (Qubit Systems), at 25°C and light intensities of 200, 400, 600 and 800 µmol photons·m-2·sec-1 respectively, set at a 12:12 day:night cycle for four days. All samples were bubbled with air at 60 ml·min-1 through a 0.2 µm stainless steel carbonating stones. Samples were collected from each tube after three days and analyzed used for assessment of photochemistry using the Aquapen-C AP-C 100 (Photon Systems Instruments). Three ml samples were placed in the dark at 25°C for a minimum of 30 minutes prior to measuring photochemistry. The NPQ1 protocol on the instrument was used for assessment of non-photochemical quenching in samples. The NPQ1 protocol administers 5 light pulses over 60 seconds during actinic light exposure, followed by 3 light pulses over 88 seconds during recovery in the dark. Blue light (455 nm) was used as actinic light in these experiments. Baseline measurements were made at f-pulse settings of 0.03 μmol · m-2 · s-1, Saturating pulses were set at 2100 μmol · m-2 · s-1, and actinic light pulses (for the NPQ1 protocol only) were set at 100, 200, 300, 400, 500, 600, 700, and 800 µmol photons·m-2·sec-1 respectively for each sample.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538602 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538602
completed
Uta Passow
University of California-Santa Barbara
709-864-8010
Ocean Sciences Centre, Memorial University Marine Lab Road, Logy Bay
St. John's
Newfoundland
A1C 5S7
Canada
uta.passow@mun.ca
pointOfContact
Edward Laws
Louisiana State University
225 578-3334
Louisiana State University School of the Coast and Environment 1002R Energy, Coast and Environment Building
Baton Rouge
LA
70803
USA
edlaws@lsu.edu
pointOfContact
Nigel D'Souza
University of California-Santa Barbara
419-819-9039
Marine Science Institute
nigel.dsouza@lifesci.ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
treatment
Fo
Fm
Fp
Fm_L1
Fm_L2
Fm_L3
Fm_L4
Fm_Lss
NPQ_L1
NPQ_L2
NPQ_L3
NPQ_L4
NPQ_Lss
NPQ_D1
NPQ_D2
NPQ_D3
Qp_L1
Qp_L2
Qp_L3
Qp_L4
Qp_Lss
Qp_D1
Qp_D2
Qp_D3
Rfd
Fm_D1
Fm_D2
Fm_D3
QY_max
QY_L1
QY_L2
QY_L3
QY_L4
QY_Lss
QY_D1
QY_D2
QY_D3
Aquapen-C AP-C 100 (Photon Systems Instruments)
Multicultivator MC-1000 OD (Qubit Systems)
theme
None, User defined
treatment
fluorescence
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Fluorometer
Cell Cultivator
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
https://www.bco-dmo.org/project/654347
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
<p>The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.</p>
<p>Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.</p>
Stressors on Marine Phytoplankton
largerWorkCitation
project
eng; USA
biota
oceans
2018-07-03
2018-07-06
0
BCO-DMO catalogue of parameters from Series 3B: Supplemental experiments on Thalassiosira pseudonana (CCMP1014) cultures determining the optimal aquapen actinic light pulse (A-pulse) setting: computed values including min and max fluorescence
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/749298.rdf
Name: treatment
Units: unitless
Description: treatment identifier of T.pseudonana; formated as Tn_Apm where 'n' is light intensity during the culture growth in micromole photons/meter^2/second and a A-pulse setting of 'm' in micromol photons/meter^2/second
http://lod.bco-dmo.org/id/dataset-parameter/749299.rdf
Name: Fo
Units: RFU (Relative Fluorscence Units)
Description: minimum fluorescence in dark-adapted state.
http://lod.bco-dmo.org/id/dataset-parameter/749300.rdf
Name: Fm
Units: RFU (Relative Fluorscence Units)
Description: the maximum fluorescence in dark-adapted state; measured during the first saturation flash after dark adaptation
http://lod.bco-dmo.org/id/dataset-parameter/749301.rdf
Name: Fp
Units: RFU (Relative Fluorscence Units)
Description: the fluorescence in the peak of fast Kautsky induction
http://lod.bco-dmo.org/id/dataset-parameter/749302.rdf
Name: Fm_L1
Units: RFU (Relative Fluorscence Units)
Description: The first measurement of the maximum fluorescence following exposure to actinic light for 7 seconds (L1 indicates the first measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749303.rdf
Name: Fm_L2
Units: RFU (Relative Fluorscence Units)
Description: The second measurement of the maximum fluorescence following exposure to actinic light for another 12 seconds (L2 indicates the second measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749304.rdf
Name: Fm_L3
Units: RFU (Relative Fluorscence Units)
Description: The third measurement of the maximum fluorescence following exposure to actinic light for another 12 seconds (L3 indicates the third measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749305.rdf
Name: Fm_L4
Units: RFU (Relative Fluorscence Units)
Description: The fourth measurement of the maximum fluorescence following exposure to actinic light for another 12 seconds (L4 indicates the fourth measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749306.rdf
Name: Fm_Lss
Units: RFU (Relative Fluorscence Units)
Description: The fifth measurement of the maximum fluorescence following exposure to actinic light for another 12 seconds (Lss indicates the fifth and final measurement in the "light" phase; represents a "steady state" or ss)
http://lod.bco-dmo.org/id/dataset-parameter/749307.rdf
Name: NPQ_L1
Units: unitless
Description: The first measurement of the non photochemical chlorophyll fluorscence quenching following exposure to actinic light for 7 seconds (L1 indicates the first measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749308.rdf
Name: NPQ_L2
Units: unitless
Description: The second measurement of the non photochemical chlorophyll fluorscence quenching following exposure to actinic light for another 12 seconds (L2 indicates the second measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749309.rdf
Name: NPQ_L3
Units: unitless
Description: The third measurement of the non photochemical chlorophyll fluorscence quenching following exposure to actinic light for another 12 seconds (L3 indicates the third measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749310.rdf
Name: NPQ_L4
Units: unitless
Description: The fourth measurement of the non photochemical chlorophyll fluorscence quenching following exposure to actinic light for another 12 seconds (L4 indicates the fourth measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749311.rdf
Name: NPQ_Lss
Units: unitless
Description: The fifth measurement of the non photochemical chlorophyll fluorscence quenching following exposure to actinic light for another 12 seconds (Lss indicates the fifth and final measurement in the "light" phase; represents a "steady state" or ss)
http://lod.bco-dmo.org/id/dataset-parameter/749312.rdf
Name: NPQ_D1
Units: unitless
Description: The first measurement of the non photochemical chlorophyll fluorscence quenching 11 seconds into the dark or recovery phase (D1 indicates the first measurement in the "dark" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749313.rdf
Name: NPQ_D2
Units: unitless
Description: The second measurement of the non photochemical chlorophyll fluorscence quenching after another 26 seconds into the dark or recovery phase (D2 indicates the second measurement in the "dark" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749314.rdf
Name: NPQ_D3
Units: unitless
Description: The third and final measurement of the non photochemical chlorophyll fluorscence quenching after another 26 seconds into the dark or recovery phase (D3 indicates the third and final measurement in the "dark" phase
http://lod.bco-dmo.org/id/dataset-parameter/749315.rdf
Name: Qp_L1
Units: unitless
Description: The first measurement of the coefficient of photochemical quenching following exposure to actinic light for 7 seconds (L1 indicates the first measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749316.rdf
Name: Qp_L2
Units: unitless
Description: The second measurement of the coefficient of photochemical quenching following exposure to actinic light for another 12 seconds (L2 indicates the second measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749317.rdf
Name: Qp_L3
Units: unitless
Description: The third measurement of the coefficient of photochemical quenching following exposure to actinic light for another 12 seconds (L3 indicates the third measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749318.rdf
Name: Qp_L4
Units: unitless
Description: The fourth measurement of the coefficient of photochemical quenching following exposure to actinic light for another 12 seconds (L4 indicates the fourth measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749319.rdf
Name: Qp_Lss
Units: unitless
Description: The fifth measurement of the coefficient of photochemical quenching following exposure to actinic light for another 12 seconds (Lss indicates the fifth and final measurement in the "light" phase; represents a "steady state" or ss)
http://lod.bco-dmo.org/id/dataset-parameter/749320.rdf
Name: Qp_D1
Units: unitless
Description: The first measurement of the coefficient of photochemical quenching 11 seconds into the dark or recovery phase (D1 indicates the first measurement in the "darkt" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749321.rdf
Name: Qp_D2
Units: unitless
Description: The second measurement of the coefficient of photochemical quenching after another 26 seconds into the dark or recovery phase (D2 indicates the second measurement in the "dark" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749322.rdf
Name: Qp_D3
Units: unitless
Description: The third and final measurement of the coefficient of photochemical quenching after another 26 seconds into the dark or recovery phase (D3 indicates the third and final measurement in the "dark" phase
http://lod.bco-dmo.org/id/dataset-parameter/749323.rdf
Name: Rfd
Units: unitless
Description: the chlorophyll fluorescence decrease ratio
http://lod.bco-dmo.org/id/dataset-parameter/749324.rdf
Name: Fm_D1
Units: RFU (Relative Fluorscence Units)
Description: The first measurement of the maximum fluorescence 11 seconds into the dark or recovery phase (D1 indicates the first measurement in the "darkt" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749325.rdf
Name: Fm_D2
Units: RFU (Relative Fluorscence Units)
Description: The second measurement of the maximum fluorescence after another 26 seconds into the dark or recovery phase (D2 indicates the second measurement in the "dark" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749326.rdf
Name: Fm_D3
Units: RFU (Relative Fluorscence Units)
Description: The third and final measurement of the maximum fluorescence after another 26 seconds into the dark or recovery phase (D3 indicates the third and final measurement in the "dark" phase
http://lod.bco-dmo.org/id/dataset-parameter/749327.rdf
Name: QY_max
Units: unitless
Description: The maximum Quantum yield. A measure of the Photosystem II efficiency. In a dark-adapted sample this is equivalent to Fv/Fm. In a light-adapted sample it is equivalent to Fv’/Fm’.
http://lod.bco-dmo.org/id/dataset-parameter/749328.rdf
Name: QY_L1
Units: unitless
Description: The first measurement of the effective quantum yield of phorosystem II following exposure to actinic light for 7 seconds (L1 indicates the first measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749329.rdf
Name: QY_L2
Units: unitless
Description: The second measurement of the effective quantum yield of phorosystem II following exposure to actinic light for another 12 seconds (L2 indicates the second measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749330.rdf
Name: QY_L3
Units: unitless
Description: The third measurement of the effective quantum yield of phorosystem II following exposure to actinic light for another 12 seconds (L3 indicates the third measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749331.rdf
Name: QY_L4
Units: unitless
Description: The fourth measurement of the effective quantum yield of phorosystem II following exposure to actinic light for another 12 seconds (L4 indicates the fourth measurement in the "light" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749332.rdf
Name: QY_Lss
Units: unitless
Description: The fifth measurement of the effective quantum yield of phorosystem II following exposure to actinic light for another 12 seconds (Lss indicates the fifth and final measurement in the "light" phase; represents a "steady state" or ss)
http://lod.bco-dmo.org/id/dataset-parameter/749333.rdf
Name: QY_D1
Units: unitless
Description: The first measurement of the effective quantum yield of phorosystem II 11 seconds into the dark or recovery phase (D1 indicates the first measurement in the "darkt" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749334.rdf
Name: QY_D2
Units: unitless
Description: The second measurement of the effective quantum yield of phorosystem II after another 26 seconds into the dark or recovery phase (D2 indicates the second measurement in the "dark" phase)
http://lod.bco-dmo.org/id/dataset-parameter/749335.rdf
Name: QY_D3
Units: unitless
Description: The third and final measurement of the effective quantum yield of phorosystem II after another 26 seconds into the dark or recovery phase (D3 indicates the third and final measurement in the "dark" phase
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6410
https://datadocs.bco-dmo.org/file/933jogkuEvKM/3B_Apulse_test_computed_trans.csv
3B_Apulse_test_computed_trans.csv
Primary data file for dataset ID 748978
download
https://www.bco-dmo.org/dataset/748978/data/download
download
onLine
dataset
<p>Experiments were conducted in the lab at the University of California Santa Barbara&nbsp;to determine the optimal actinic light pulse (A-pulse) setting for NPQ1 protocols of the AquaPen for assessment of non-photochemical quenching in Thalassiosira pseudonana CCMP 1014 cultures grown at different light environments. TP1014 stock cultures were maintained in artificial sea water (Kester et. al 1967), enriched as with f/2 media (Guillard 1975). For the experiment, 5 ml of the TP1014 stock cultures was inoculated into 75 ml of ASW in four tubes. The tubes were incubated in a Multicultivator MC-1000 OD unit (Qubit Systems), at 25°C and light intensities of 200, 400, 600 and 800 µmol photons·m-2·sec-1 respectively, set at a 12:12 day:night cycle for four days. All samples were bubbled with air at 60 ml·min-1 through a 0.2 µm stainless steel carbonating stones. Samples were collected from each tube after three days and analyzed used for assessment of photochemistry using the Aquapen-C AP-C 100 (Photon Systems Instruments). Three ml samples were placed in the dark at 25°C for a minimum of 30 minutes prior to measuring photochemistry. The NPQ1 protocol on the instrument was used for assessment of non-photochemical quenching in samples. The NPQ1 protocol administers 5 light pulses over 60 seconds during actinic light exposure, followed by 3 light pulses over 88 seconds during recovery in the dark. Blue light (455 nm) was used as actinic light in these experiments. Baseline measurements were made at f-pulse settings of 0.03 μmol · m-2 · s-1, Saturating pulses were set at 2100 μmol · m-2 · s-1, and actinic light pulses (for the NPQ1 protocol only) were set at 100, 200, 300, 400, 500, 600, 700, and 800 µmol photons·m-2·sec-1 respectively for each sample.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing Notes:<br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Aquapen-C AP-C 100 (Photon Systems Instruments)
Aquapen-C AP-C 100 (Photon Systems Instruments)
PI Supplied Instrument Name: Aquapen-C AP-C 100 (Photon Systems Instruments) PI Supplied Instrument Description:Used to measure fluorescence.
A hand-held cuvette version of the FluorPen fluorometer equipped with a blue and red LED emitter. Blue excitation light (455 nm) is intended for chlorophyll excitation, i.e., for measuring chlorophyll fluorescence in algal cultures. Red-orange excitation light (620 nm) is intended for excitation through phycobilins and is suitable for measuring in cyanobacteria. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Multicultivator MC-1000 OD (Qubit Systems)
Multicultivator MC-1000 OD (Qubit Systems)
PI Supplied Instrument Name: Multicultivator MC-1000 OD (Qubit Systems) PI Supplied Instrument Description:Used for incubation of TP1014 cultures. Instrument Name: Cell Cultivator Instrument Short Name: Instrument Description: An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.