http://lod.bco-dmo.org/id/dataset/749140 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2018-11-01 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) growth under bubbling stress: NPQ1 protocol (Non-Photochemical chlorophyll fluorescence Quenching) raw fluorescence measurements for non-aerated samples and aerated samples 2018-10-31 publication 2018-10-31 revision BCO-DMO Linked Data URI 2018-10-31 creation http://lod.bco-dmo.org/id/dataset/749140 Uta Passow University of California-Santa Barbara principalInvestigator Edward Laws Louisiana State University College of the Coast and Environment principalInvestigator Nigel D'Souza University of California-Santa Barbara principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Passow, U., Laws, E., D'Souza, N. (2018) Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) growth under bubbling stress: NPQ1 protocol (Non-Photochemical chlorophyll fluorescence Quenching) raw fluorescence measurements for non-aerated samples and aerated samples. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-10-31 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/749140 [access date] Series 3B: bubble expt NPQ raw Dataset Description: &lt;p&gt;Experiments were conducted to investigate the impact of bubbling on the growth yield of Thalassiosira pseudonana CCMP 1014 grown in 80 ml culture tubes. This dataset includes NPQ1 protocol (Non-Photochemical chlorophyll fluorescence Quenching)&amp;nbsp;raw fluorescence results for non-aerated samples and aerated samples.&lt;/p&gt; Methods and Sampling: &lt;p&gt;Experiments were conducted to investigate the impact of bubbling gas through cultures of Thalassiosira pseudonana CCMP 1014 grown in Multicultivator MC-1000 OD culture tubes. TP1014 stock cultures were maintained in artificial seawater (Kester et. al 1967), enriched as with f/2 media (Guillard 1975). For the experiment, 5 ml of the TP1014 stock cultures were inoculated into 75 ml of ASW in eight tubes. The tubes were incubated in a Multicultivator MC-1000 OD unit (Qubit Systems), at 25 deg C and 400 µmol photons * m-2 * sec-1, set at a 12:12 day:night cycle for four days. Three tubes had no aeration (T1, T2, and T3); three tubes were bubbled with air at 60 ml·min-1 through a 0.2 µm stainless steel “carbonating stone” (T4, T5, and T6); and two tubes were bubbled with air at 120 ml·min-1 through a 0.2 µm stainless steel “carbonating stone” (T7 and T8). Samples were collected from each tube at the start of the experiment (day-0), and 5 hours after the start of the light phase each day (i.e. at 24-hour intervals) after that for four days. Samples were always collected 5 hours after the start of the light phase.&lt;/p&gt; &lt;p&gt;Three ml of samples from the non-aerated (T1, T2, and T3) and the 60 ml·min-1 aeration tubes (T4, T5, and T6) were used for assessment of photochemistry using the Aquapen-C AP-C 100 (Photon Systems Instruments). Samples were placed in the dark at 25 deg C for a minimum of 30 minutes prior to measuring photochemistry. The NPQ1 protocol on the instrument was used for assessment of non-photochemical quenching in samples, and the LC3 protocol was used to generate light curves that provide measurements of photosynthesis rates. The NPQ1 protocol administers 5 light pulses over 60 seconds during actinic light exposure, followed by 3 light pulses over 88 seconds during recovery in the dark. The LC3 protocol involves measurements of baseline fluorescence and maximal fluorescence during 7 phases of 60 seconds each, with each phase representing a light intensity from 10 to 1000 μmol * m-2 * s-1. Blue light (455 nm) was used as actinic light in these experiments. Baseline measurements were made at 0.03 μmol * m-2 * s-1, Saturating pulses were set at 2100 μmol * m-2 * s-1, and actinic light pulses (for the NPQ1 protocol only) were set at 1000 μmol * m-2 * s-1.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538602 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538602 completed Uta Passow University of California-Santa Barbara 709-864-8010 Ocean Sciences Centre, Memorial University Marine Lab Road, Logy Bay St. John's Newfoundland A1C 5S7 Canada uta.passow@mun.ca pointOfContact Edward Laws Louisiana State University College of the Coast and Environment 225 578-3334 Louisiana State University School of the Coast and Environment 1002R Energy, Coast and Environment Building Baton Rouge LA 70803 USA edlaws@lsu.edu pointOfContact Nigel D'Souza University of California-Santa Barbara 419-819-9039 Marine Science Institute nigel.dsouza@lifesci.ucsb.edu pointOfContact asNeeded Dataset Version: 1 Unknown time_usec Non_Areated_T1 Non_Areated_T2 Non_Areated_T3 Areated_T4 Areated_T5 Areated_T6 Multicultivator MC-1000 OD (Qubit Systems) Guava easyCyte HT Sampling Flow Cytometer Aquapen-C AP-C 100 (Photon Systems Instruments) theme None, User defined time_elapsed fluorescence featureType BCO-DMO Standard Parameters Cell Cultivator Flow Cytometer Fluorometer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Effects of multiple stressors on Marine Phytoplankton https://osprey.bco-dmo.org/project/654347 Collaborative Research: Effects of multiple stressors on Marine Phytoplankton <p>The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.</p> <p>Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.</p> Stressors on Marine Phytoplankton largerWorkCitation project eng; USA oceans 2018-07-03 2018-07-06 0 BCO-DMO catalogue of parameters from Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) growth under bubbling stress: NPQ1 protocol (Non-Photochemical chlorophyll fluorescence Quenching) raw fluorescence measurements for non-aerated samples and aerated samples Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/749153.rdf Name: time_usec Units: microseconds Description: <p>time elapsed since beginning of run</p> http://lod.bco-dmo.org/id/dataset-parameter/749154.rdf Name: Non_Areated_T1 Units: relative fluorescence units (RFU) Description: <p>fluorescence measurements from the NPQ1 protocol of the AquaPen for un-aerated treatment T1</p> http://lod.bco-dmo.org/id/dataset-parameter/749155.rdf Name: Non_Areated_T2 Units: relative fluorescence units (RFU) Description: <p>fluorescence measurements from the NPQ1 protocol of the AquaPen for un-aerated treatment T2</p> http://lod.bco-dmo.org/id/dataset-parameter/749156.rdf Name: Non_Areated_T3 Units: relative fluorescence units (RFU) Description: <p>fluorescence measurements from the NPQ1 protocol of the AquaPen for un-aerated treatment T3</p> http://lod.bco-dmo.org/id/dataset-parameter/749157.rdf Name: Areated_T4 Units: relative fluorescence units (RFU) Description: <p>fluorescence measurements from the NPQ1 protocol of the AquaPen for aerated treatment T1</p> http://lod.bco-dmo.org/id/dataset-parameter/749158.rdf Name: Areated_T5 Units: relative fluorescence units (RFU) Description: <p>fluorescence measurements from the NPQ1 protocol of the AquaPen for aerated treatment T2</p> http://lod.bco-dmo.org/id/dataset-parameter/749159.rdf Name: Areated_T6 Units: relative fluorescence units (RFU) Description: <p>fluorescence measurements from the NPQ1 protocol of the AquaPen for aerated treatment T3</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 6635 https://datadocs.bco-dmo.org/file/xooyA1RfmnlXAV/3B_bubble_NPQ_raw.csv 3B_bubble_NPQ_raw.csv Primary data file for dataset ID 749140 download https://osprey.bco-dmo.org/dataset/749140/data/download download onLine dataset &lt;p&gt;Experiments were conducted to investigate the impact of bubbling gas through cultures of Thalassiosira pseudonana CCMP 1014 grown in Multicultivator MC-1000 OD culture tubes. TP1014 stock cultures were maintained in artificial seawater (Kester et. al 1967), enriched as with f/2 media (Guillard 1975). For the experiment, 5 ml of the TP1014 stock cultures were inoculated into 75 ml of ASW in eight tubes. The tubes were incubated in a Multicultivator MC-1000 OD unit (Qubit Systems), at 25 deg C and 400 µmol photons * m-2 * sec-1, set at a 12:12 day:night cycle for four days. Three tubes had no aeration (T1, T2, and T3); three tubes were bubbled with air at 60 ml·min-1 through a 0.2 µm stainless steel “carbonating stone” (T4, T5, and T6); and two tubes were bubbled with air at 120 ml·min-1 through a 0.2 µm stainless steel “carbonating stone” (T7 and T8). Samples were collected from each tube at the start of the experiment (day-0), and 5 hours after the start of the light phase each day (i.e. at 24-hour intervals) after that for four days. Samples were always collected 5 hours after the start of the light phase.&lt;/p&gt; &lt;p&gt;Three ml of samples from the non-aerated (T1, T2, and T3) and the 60 ml·min-1 aeration tubes (T4, T5, and T6) were used for assessment of photochemistry using the Aquapen-C AP-C 100 (Photon Systems Instruments). Samples were placed in the dark at 25 deg C for a minimum of 30 minutes prior to measuring photochemistry. The NPQ1 protocol on the instrument was used for assessment of non-photochemical quenching in samples, and the LC3 protocol was used to generate light curves that provide measurements of photosynthesis rates. The NPQ1 protocol administers 5 light pulses over 60 seconds during actinic light exposure, followed by 3 light pulses over 88 seconds during recovery in the dark. The LC3 protocol involves measurements of baseline fluorescence and maximal fluorescence during 7 phases of 60 seconds each, with each phase representing a light intensity from 10 to 1000 μmol * m-2 * s-1. Blue light (455 nm) was used as actinic light in these experiments. Baseline measurements were made at 0.03 μmol * m-2 * s-1, Saturating pulses were set at 2100 μmol * m-2 * s-1, and actinic light pulses (for the NPQ1 protocol only) were set at 1000 μmol * m-2 * s-1.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;BCO-DMO Processing Notes:&lt;br /&gt; - added conventional header with dataset name, PI name, version date&lt;br /&gt; - modified parameter names to conform with BCO-DMO naming conventions&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Multicultivator MC-1000 OD (Qubit Systems) Multicultivator MC-1000 OD (Qubit Systems) PI Supplied Instrument Name: Multicultivator MC-1000 OD (Qubit Systems) PI Supplied Instrument Description:Used for incubation of TP1014 cultures. Instrument Name: Cell Cultivator Instrument Short Name: Instrument Description: An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction. Guava easyCyte HT Sampling Flow Cytometer Guava easyCyte HT Sampling Flow Cytometer PI Supplied Instrument Name: Guava easyCyte HT Sampling Flow Cytometer PI Supplied Instrument Description:Used to count cells. Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ Aquapen-C AP-C 100 (Photon Systems Instruments) Aquapen-C AP-C 100 (Photon Systems Instruments) PI Supplied Instrument Name: Aquapen-C AP-C 100 (Photon Systems Instruments) PI Supplied Instrument Description:Used to measure fluorescence. A hand-held cuvette version of the FluorPen fluorometer equipped with a blue and red LED emitter. Blue excitation light (455 nm) is intended for chlorophyll excitation, i.e., for measuring chlorophyll fluorescence in algal cultures. Red-orange excitation light (620 nm) is intended for excitation through phycobilins and is suitable for measuring in cyanobacteria. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/