<div><p>Experiments were conducted to investigate the impact of bubbling gas through cultures of Thalassiosira pseudonana CCMP 1014 grown in Multicultivator MC-1000 OD culture tubes. TP1014 stock cultures were maintained in artificial seawater (Kester et. al 1967), enriched as with f/2 media (Guillard 1975). For the experiment, 5 ml of the TP1014 stock cultures were inoculated into 75 ml of ASW in eight tubes. The tubes were incubated in a Multicultivator MC-1000 OD unit (Qubit Systems), at 25 deg C and 400 µmol photons * m-2 * sec-1, set at a 12:12 day:night cycle for four days. Three tubes had no aeration (T1, T2, and T3); three tubes were bubbled with air at 60 ml·min-1 through a 0.2 µm stainless steel “carbonating stone” (T4, T5, and T6); and two tubes were bubbled with air at 120 ml·min-1 through a 0.2 µm stainless steel “carbonating stone” (T7 and T8). Samples were collected from each tube at the start of the experiment (day-0), and 5 hours after the start of the light phase each day (i.e. at 24-hour intervals) after that for four days. Samples were always collected 5 hours after the start of the light phase.</p>
<p>Three ml of samples from the non-aerated (T1, T2, and T3) and the 60 ml·min-1 aeration tubes (T4, T5, and T6) were used for assessment of photochemistry using the Aquapen-C AP-C 100 (Photon Systems Instruments). Samples were placed in the dark at 25 deg C for a minimum of 30 minutes prior to measuring photochemistry. The NPQ1 protocol on the instrument was used for assessment of non-photochemical quenching in samples, and the LC3 protocol was used to generate light curves that provide measurements of photosynthesis rates. The NPQ1 protocol administers 5 light pulses over 60 seconds during actinic light exposure, followed by 3 light pulses over 88 seconds during recovery in the dark. The LC3 protocol involves measurements of baseline fluorescence and maximal fluorescence during 7 phases of 60 seconds each, with each phase representing a light intensity from 10 to 1000 μmol * m-2 * s-1. Blue light (455 nm) was used as actinic light in these experiments. Baseline measurements were made at 0.03 μmol * m-2 * s-1, Saturating pulses were set at 2100 μmol * m-2 * s-1, and actinic light pulses (for the NPQ1 protocol only) were set at 1000 μmol * m-2 * s-1.</p></div>
Series 3B: bubble expt LC raw
<div><p>Experiments were conducted to investigate the impact of bubbling on the growth yield of Thalassiosira pseudonana CCMP 1014 grown in 80 ml culture tubes. This dataset includes LC3 protocol (...) raw fluorescence results for non-aerated samples and aerated samples.</p></div>
Series 3B: bubble expt LC raw
<div><p>BCO-DMO Processing Notes:<br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p></div>
749211
Series 3B: bubble expt LC raw
2018-11-01T09:44:14-04:00
2018-11-01T09:44:14-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:749211
Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) growth under bubbling stress: LC3 protocol raw fluorescence measurements for non-aerated samples and aerated samples
Experiments were conducted to investigate the impact of bubbling on the growth yield of Thalassiosira pseudonana CCMP 1014 grown in 80 ml culture tubes. This dataset includes raw fluorescence measurements for non-aerated samples and aerated samples using LC3 protocol.
false
Passow, U., Laws, E., D'Souza, N. (2018) Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) growth under bubbling stress: LC3 protocol raw fluorescence measurements for non-aerated samples and aerated samples. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-10-31 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/749211 [access date]
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2019-07-01
2018-10-31
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