http://lod.bco-dmo.org/id/dataset/749665 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2018-11-14 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Metagenetic amplicon sequence data for multiple coral species from Curacao, Australia and Hawaii 2018-11-19 publication 2018-11-19 revision BCO-DMO Linked Data URI 2018-11-19 creation http://lod.bco-dmo.org/id/dataset/749665 Michael P. Lesser University of New Hampshire principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Lesser, M. P. (2018) Metagenetic amplicon sequence data for multiple coral species from Curacao, Australia and Hawaii. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-11-19 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/749665 [access date] Dataset Description: &lt;p&gt;These data can be found by accessing the NCBI Sequence Read Archive BioProject ID: PRJNA498285 (http://www.ncbi.nlm.nih.gov/bioproject/498285, last accessed 2018-11-14).&lt;/p&gt; &lt;p&gt;Metagenetic amplicon sequence FASTA file for multiple coral species from Heron Island, Great Barrier Reef, Australia (23.4423° S, 151.9148° E), Hawaii (21.4433° N, 157.7490° W), and Curacao (12.1696° N, 68.9900° W).&lt;/p&gt; Methods and Sampling: &lt;p&gt;Samples of corals were collected on shallow reefs in Australia, Hawaii and Curacao and preserved in DNA /RNA preservation buffer, Genomic DNA and RNA were extracted and sequenced.&amp;nbsp; Sequences were assembled and annotated as required for each dataset.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1437054 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1437054 completed Michael P. Lesser University of New Hampshire 603-862-3442 Department of Molecular, Cellular and Biomedical Sciences Durham NH 03824 USA mpl@unh.edu pointOfContact asNeeded Dataset Version: 1 Unknown Illumina MySeq theme None, User defined Automated DNA Sequencer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Nitrogen Fixing Prokaryotes in Corals: Is Nitrogen Fixation a Core Function of the Coral Microbiome? https://osprey.bco-dmo.org/project/645509 Nitrogen Fixing Prokaryotes in Corals: Is Nitrogen Fixation a Core Function of the Coral Microbiome? <p><em>Description from NSF award abstract:</em><br /> Coral reefs provide crucial services for people and are becoming increasingly threatened by climate change. But many important questions about coral reefs remain unanswered, such as how their incredibly high biodiversity has developed and is maintained. It is well known that reef-forming corals consist of many different groups of bacteria and algae that live in the tissues of corals, but the function of most of these microbes remain unknown. One of the reported microbial functions is the process called nitrogen fixation. Nitrogen fixation is better known as a very important process on land. For example, alfalfa in agricultural fields has microbes associated with its roots that can capture nitrogen from the air and replace nitrogen lost from soils in water. The nitrogen cycle in the oceans is less well known, especially in tropical waters where nitrogen levels are low, although nitrogen in coral reef systems is known to derive mainly from nitrogen fixation. Recently nitrogen-fixing bacteria have been discovered in the tissues of corals, which begs the overarching question of this project: how important is the contribution of this fixed nitrogen to corals and to the surrounding environment? This research will integrate with an educational program that fosters critical thinking and cooperative learning among participants by involving undergraduate students who will work closely with the scientists. Students from underrepresented backgrounds will be recruited; the undergraduates will become part of an existing undergraduate research opportunities program where they receive mentoring, financial support, and intensive writing support while conducting original research projects of their own. Also a new program will be developed with the university's veterans office to attract and transition veterans into science fields across campus. Finally, outreach activities will be organized at local public high schools, and some public environmental education lectures about the ecology and biology of coral reefs are planned.</p> <p>Significant gaps in understanding exist about the role of prokaryotes in the physiology, biochemistry and ecology of reef-forming corals. This project will assess how different prokaryotic communities affect the biogeochemistry of nitrogen, specifically nitrogen fixation, within the coral holobiont and how nitrogen fixation contributes to the dissolved organic nitrogen (DON) content of excreted mucus. The introduction of "new nitrogen" though nitrogen fixation and release of DON could have significant impacts on the biogeochemistry of nitrogen in the adjacent benthic communities surrounding corals. High throughput sequencing tools will be used to quantify the taxonomic and functional diversity of these symbioses in corals from the Pacific and Caribbean, as well as the taxonomic diversity of nifH genes in the tissues of corals. Rates of nitrogen fixation on several coral species from Australia, Hawaii and Curacao will be measured, along with the translocation of fixed nitrogen to all compartments of the coral holobiont, and the contribution of "new nitrogen" to the DON of mucus released to the environment determined. The results will increase our understanding of the taxonomic and functional biodiversity of symbiotic prokaryotes in corals. This project will also help guide future studies on the biogeochemical cycling of coral-derived nitrogen on coral reefs.</p> NitroFixCorals largerWorkCitation project eng; USA oceans -157.749 151.9148 -23.4423 21.4433 2018-11-19 Heron Island, Australia; Kaneohe Bay, Hawaii; Curacao 0 BCO-DMO catalogue of parameters from Metagenetic amplicon sequence data for multiple coral species from Curacao, Australia and Hawaii Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://osprey.bco-dmo.org/dataset/749665/data/download download onLine dataset &lt;p&gt;Samples of corals were collected on shallow reefs in Australia, Hawaii and Curacao and preserved in DNA /RNA preservation buffer, Genomic DNA and RNA were extracted and sequenced.&amp;nbsp; Sequences were assembled and annotated as required for each dataset.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;fastq base calling and quality control&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Illumina MySeq Illumina MySeq PI Supplied Instrument Name: Illumina MySeq PI Supplied Instrument Description:Genomic DNA and RNA were extracted and sequenced using Illumina MySeq Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences.