http://lod.bco-dmo.org/id/dataset/749722
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-11-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Total protein concentrations from study of sponge disease transmission from the Exuma Cays, Bahamas from 2009-2014
2018-11-16
publication
2018-11-16
revision
BCO-DMO Linked Data URI
2018-11-16
creation
http://lod.bco-dmo.org/id/dataset/749722
Deborah J. Gochfeld
University of Mississippi
principalInvestigator
Marilyn Brandt
University of the Virgin Islands Center for Marine and Environmental Studies
principalInvestigator
Julie Olson
University of Alabama-Tuscaloosa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gochfeld, D. J., Olson, J., Brandt, M. (2018) Total protein concentrations from study of sponge disease transmission from the Exuma Cays, Bahamas from 2009-2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-11-16 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/749722 [access date]
Total Protein data from study of sponge disease transmission. Dataset Description: <p>Total protein data from study of sponge disease transmission. Temporal changes in biochemical, physiological and microbiological associations in the <em>Aplysina cauliformis</em> holobiont over the course of transmission of <em>Aplysina</em> Red Band Disease (ARBS) were investigated in two experiments, one performed in July and one performed in January.</p>
<p><strong>Related datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/641052" target="_blank">Sponge Disease Transmission - Secondary Metabolites</a><br />
<a href="http://www.bco-dmo.org/dataset/641090" target="_blank">Sponge Disease Transmission - HSP70</a><br />
<a href="https://www.bco-dmo.org/dataset/640933" target="_blank">Sponge Disease Transmission - Chl-a</a> <a href="https://www.bco-dmo.org/dataset/749753" target="_blank">Sponge Disease Transmission - T-REX</a></p> Methods and Sampling: <p>Temporal changes in biochemical, physiological and microbiological associations in the <em>Aplysina cauliformis </em>holobiont over the course of transmission of <em>Aplysina</em> Red Band Disease (ARBS) were investigated in two experiments, one performed in July and one performed in January In both experiments, healthy and ARBS-affected sponges (n = 21-24 of each) were marked <em>in situ </em>at North Norman’s reef in the Exuma Cays, Bahamas. Healthy sponges were collected, labeled and a subsample (10 cm) was removed into an individual resealable plastic bag underwater to serve as an "initial" sample. The remainder of each of these healthy sponges was randomly attached to either a healthy <em>in situ</em> sample or to the active red band on a diseased <em>in situ </em>sample using a cable tie. In July, randomly selected pairs of healthy-healthy and healthy-diseased sponges were collected on days 3, 6 and 9 to provide "final" samples. In January, randomly selected pairs of each type were collected on days 1, 3 and 9. Following final collection, all pairs were separated in the lab, and photographs were taken to confirm whether ARBS transmission had occurred.</p>
<p>Following collection of initial and final samples, the sponges were subsampled for several analyses. Only sections of healthy tissue were used, even from the ARBS-affected sponges (Gochfeld et al. 2012, Marine Ecology Progress Series; Gochfeld et al. 2012, Journal of Chemical Ecology; Olson et al. 2014). Subsamples were collected for measurement of chlorophyll a, total protein, secondary metabolite profiles, and microbial community analysis. In addition, in January, subsamples were also collected for heat shock protein 70 expression analysis.</p>
<p>Analytical methods followed those of Gochfeld et al. (2012, Marine Ecology Progress Series) for chlorophyll <em>a </em>and total protein, and Olson et al. (2014) for microbial community assemblages. Secondary metabolite profiles used similar methods to those described in Gochfeld et al. (2012, Journal of Chemical Ecology) except that the samples were extracted three times in methanol prior to generating chemical profiles using HPLC. Areas under the curve for peaks that were consistently found in either <em>in situ</em> healthy and/or <em>in situ </em>diseased sponges were quantified. Heat shock protein 70 expression analysis was performed following methods in Sarkis et al. (2005).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1214303 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1214303
completed
Deborah J. Gochfeld
University of Mississippi
662-915-6769
National Center for Natural Products Research P.O. Box 1848
University
MS
38677
USA
gochfeld@olemiss.edu
pointOfContact
Marilyn Brandt
University of the Virgin Islands Center for Marine and Environmental Studies
340-693-1379
2 John Brewers Bay
St. Thomas, USVI
00802
mbrandt@uvi.edu
pointOfContact
Julie Olson
University of Alabama-Tuscaloosa
205-348-2633
801 University Blvd. Box 870344
Tuscaloosa
AL
35487
USA
jolson@biology.as.ua.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
month
location
lat
lon
sample_type
treatment
sponge
day_final_collected
total_protein_initial
total_protein_final
theme
None, User defined
month of year
site
latitude
longitude
sample type
treatment
No BCO-DMO term
protein concentration
featureType
BCO-DMO Standard Parameters
Camera
instrument
BCO-DMO Standard Instruments
Bahamas_Gochfeld
service
Deployment Activity
Exuma Cays, Bahamas
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Developing a model for transmission of an infectious disease of marine sponges
https://osprey.bco-dmo.org/project/559372
Developing a model for transmission of an infectious disease of marine sponges
<p><em>Description from NSF award abstract:</em><br />
Diseases of marine invertebrates have been shown to be primary causes of the accelerating destruction of Caribbean coral reef systems. Diseases affecting natural populations threaten biodiversity, resilience and the ecological balance of communities, as well as the ecosystem services they provide. To date, most studies of diseases on reefs have focused on corals, however, reports of sponge diseases have also increased across the globe. On Caribbean reefs, sponges are often a dominant component of the reef biomass,and thus play an important role in the ecology of these ecosystems. The most well described disease affecting Caribbean sponges is Aplysina Red Band Syndrome (ARBS), which affects sponges of the genus Aplysina, resulting in reduced growth, tissue necrosis and breakage at the site of the lesion, particularly during storm events. Understanding how diseases emerge and are transmitted within marine ecosystems is critical for maintaining a healthy level of biodiversity, particularly if we are to gain any predictive power in a rapidly-changing environment. Testing models of disease transmission using extensive field observations and laboratory analyses will contribute to a better understanding of disease processes and developing a transmission model for ARBS requires detailed knowledge about the pathogen-host interaction and pathogen reservoirs in the environment. While a large body of information regarding the ecology and physiology of ARBS-infected sponges is available it is recognized that modeling the transmission dynamics requires a more focused and collaborative approach. This project will develop and test a model of marine disease processes that includes the role of polymicrobial infections, sources and sinks of the pathogen(s), and the ontogeny of this disease within a model host sponge species (<em>Aplysina cauliformis</em>).</p>
<p>This novel approach is a high-risk venture (i.e., a timely idea lacking requisite results) with high pay-off potential (i.e., the results will fundamentally enhance our understanding of disease transmission within marine sponges). In this respect, the proposal is appropriate for EAGER funding. The principal investigators will use modern techniques such as high throughput sequencing and incorporate these approaches as a new tools in their laboratories as well as in their undergraduate and graduate courses. Graduate and undergraduate students will also be provided with multidisciplinary hands-on research experiences and will participate in sponge disease surveys to test the newly developed transmission model. Public seminars will be presented to discuss the implications of marine diseases coral reefs and to highlight the potential utility of disease models for the effective management of marine resources. Results from the proposed research will further our knowledge of disease transmission dynamics and enhance our understanding of the role of diseases in the ecology of coral reef ecosystems.</p>
<p><strong>Selected publications related to this research:</strong><br />
Olson JB, Thacker RW, Gochfeld DJ (2014) Molecular community profiling reveals impacts of time, space, and disease status on the bacterial community associated with the Caribbean sponge <em>Aplysina cauliformis</em>. FEMS Microbiology Ecology 87:268-279. doi:<a href="http://dx.doi.org/10.1111/1574-6941.12222" target="_blank">10.1111/1574-6941.12222</a></p>
<p>Easson CG, Slattery M, Momm HG, Olson JB, Thacker RW, Gochfeld DJ (2013) Exploring individual- to population-level impacts of disease on coral reef sponges: using spatial analysis to assess the fate, dynamics, and transmission of <em>Aplysina</em> Red Band Syndrome (ARBS). PLoS One 8(11): e79976. doi:<a href="http://dx.doi.org/10.1371/journal.pone.0079976" target="_blank">10.1371/journal.pone.0079976</a></p>
<p>Gochfeld DJ, Easson CG, Freeman CJ, Thacker RW, Olson JB (2012) Disease and nutrient enrichment as potential stressors on the Caribbean sponge <em>Aplysina cauliformis </em>and its bacterial symbionts. Marine Ecology Progress Series 456:101-111. doi:<a href="http://dx.doi.org/10.3354/meps09716" target="_blank"><span style="font-size:12px">10.3354/meps09716 </span></a></p>
<p>Gochfeld DJ, Kamel HN, Olson JB, Thacker RW (2012) Trade-offs in defensive metabolite production but not ecological function in healthy and diseased sponges. Journal of Chemical Ecology 38:451-462. doi:<a href="http://dx.doi.org/10.1007/s10886-012-0099-5" target="_blank">10.1007/s10886-012-0099-5</a></p>
<p>Gochfeld DJ, Schlöder C, Thacker RW (2007) Sponge Community Structure and Disease Prevalence on coral reefs in Bocas del Toro, Panama. In: Custódio MR, Lõbo-Hajdu G, Hajdu E, Muricy G (eds) <em>Porifera Research: Biodiversity, Innovation, and Sustainability</em>. Série Livros 28. Museu Nacional, Rio de Janeiro. Pp 335-343. URL: <a href="http://hdl.handle.net/10088/12017" target="_blank">http://hdl.handle.net/10088/12017 </a></p>
<p>Olson J, Gochfeld D, Slattery M (2006) <em>Aplysina</em> red band syndrome: a new threat to Caribbean sponges. Diseases of Aquatic Organisms 71:163-168. doi:<a href="http://dx.doi.org/10.3354/dao071163" target="_blank">10.3354/dao071163</a></p>
Sponge Disease Model
largerWorkCitation
project
eng; USA
oceans
Exuma Cays, Bahamas
-76.13788
-76.13788
23.7898
23.7898
2018-11-16
Exuma, Bahamas; Carrie Bow Cay, Belize; St. Thomas, USVI
0
BCO-DMO catalogue of parameters from Total protein concentrations from study of sponge disease transmission from the Exuma Cays, Bahamas from 2009-2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/749734.rdf
Name: month
Units: dimensionless
Description: <p>Month when experiment was conducted.</p>
http://lod.bco-dmo.org/id/dataset-parameter/749735.rdf
Name: location
Units: dimensionless
Description: <p>Location where experiment was conducted.</p>
http://lod.bco-dmo.org/id/dataset-parameter/749736.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude of experiment location.</p>
http://lod.bco-dmo.org/id/dataset-parameter/749737.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude of experiment location.</p>
http://lod.bco-dmo.org/id/dataset-parameter/749738.rdf
Name: sample_type
Units: dimensionless
Description: <p>Description of experiment/sample type: attached sponges or in-situ.</p>
http://lod.bco-dmo.org/id/dataset-parameter/749739.rdf
Name: treatment
Units: coded/dimensionless
Description: <p>Treatment type. For attached sponges: 1=attached to healthy; 2=attached to diseased. For in-situ sponges: 1=healthy; 2=diseased.</p>
http://lod.bco-dmo.org/id/dataset-parameter/749740.rdf
Name: sponge
Units: dimensionless
Description: <p>Sponge identifier.</p>
http://lod.bco-dmo.org/id/dataset-parameter/749741.rdf
Name: day_final_collected
Units: coded/dimensionless
Description: <p>Final collection day. 1=final collected on day 1; 3=final collected on day 3; 6=final collected on day 6; 9=final collected on day 9.</p>
http://lod.bco-dmo.org/id/dataset-parameter/749746.rdf
Name: total_protein_initial
Units: micrograms protein per milligram sponge tissue (ug/mg)
Description: <p>Initial total protein</p>
http://lod.bco-dmo.org/id/dataset-parameter/749747.rdf
Name: total_protein_final
Units: micrograms protein per milligram sponge tissue (ug/mg)
Description: <p>Final total protein</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6588
https://datadocs.bco-dmo.org/file/nGGl0OLF3WZV6Q/sponge_disease_protein.csv
sponge_disease_protein.csv
Primary data file for dataset ID 749722
download
https://osprey.bco-dmo.org/dataset/749722/data/download
download
onLine
dataset
<p>Temporal changes in biochemical, physiological and microbiological associations in the <em>Aplysina cauliformis </em>holobiont over the course of transmission of <em>Aplysina</em> Red Band Disease (ARBS) were investigated in two experiments, one performed in July and one performed in January In both experiments, healthy and ARBS-affected sponges (n = 21-24 of each) were marked <em>in situ </em>at North Norman’s reef in the Exuma Cays, Bahamas. Healthy sponges were collected, labeled and a subsample (10 cm) was removed into an individual resealable plastic bag underwater to serve as an "initial" sample. The remainder of each of these healthy sponges was randomly attached to either a healthy <em>in situ</em> sample or to the active red band on a diseased <em>in situ </em>sample using a cable tie. In July, randomly selected pairs of healthy-healthy and healthy-diseased sponges were collected on days 3, 6 and 9 to provide "final" samples. In January, randomly selected pairs of each type were collected on days 1, 3 and 9. Following final collection, all pairs were separated in the lab, and photographs were taken to confirm whether ARBS transmission had occurred.</p>
<p>Following collection of initial and final samples, the sponges were subsampled for several analyses. Only sections of healthy tissue were used, even from the ARBS-affected sponges (Gochfeld et al. 2012, Marine Ecology Progress Series; Gochfeld et al. 2012, Journal of Chemical Ecology; Olson et al. 2014). Subsamples were collected for measurement of chlorophyll a, total protein, secondary metabolite profiles, and microbial community analysis. In addition, in January, subsamples were also collected for heat shock protein 70 expression analysis.</p>
<p>Analytical methods followed those of Gochfeld et al. (2012, Marine Ecology Progress Series) for chlorophyll <em>a </em>and total protein, and Olson et al. (2014) for microbial community assemblages. Secondary metabolite profiles used similar methods to those described in Gochfeld et al. (2012, Journal of Chemical Ecology) except that the samples were extracted three times in methanol prior to generating chemical profiles using HPLC. Areas under the curve for peaks that were consistently found in either <em>in situ</em> healthy and/or <em>in situ </em>diseased sponges were quantified. Heat shock protein 70 expression analysis was performed following methods in Sarkis et al. (2005).</p>
Specified by the Principal Investigator(s)
<p>Chlorophyll-a<em> </em>and protein concentrations, areas under the curve of secondary metabolite peaks, and HSP70 band intensity from healthy sponges attached to either healthy or diseased <em>in situ </em>sponges were compared using repeated measures ANCOVA, with time (initial vs. final) and treatment (attached to a healthy or diseased sponge) as the main factors, and the number of days of attachment (3, 6, 9 in July; 1, 3, 9 in January) as the covariate. For the <em>in situ</em> sponges from which only final samples were collected, parameters were compared using a one-way ANCOVA with treatment (initially healthy or diseased) as the main factor and day collected (equivalent to number of days of attachment: 3, 6, 9 in July; 1, 3, 9 in January) as the covariate. Microbial community assemblage data were analyzed using T-REX and PRIMER, as described in Olson et al. (2014).</p>
<p>BCO-DMO Processing:<br />
- Reorganized data into one table (rather than 2);<br />
- Added "sample_type" column;<br />
- Added location information provided on metadata form, converted lat/lon to decimal degrees,<br />
- 2018-Nov-16: removed embargo on dataset.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
Deployment: Bahamas_Gochfeld
Bahamas_Gochfeld
Gochfeld_lab
laboratory
Bahamas_Gochfeld
Deborah J. Gochfeld
University of Mississippi
Gochfeld_lab
laboratory