http://lod.bco-dmo.org/id/dataset/749774
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-11-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Genotype data for E. lori individuals sequenced at 71 microsatellites from a 41 km-long transect of the Belize Barrier reef during 2018
2018-10-22
publication
2018-10-22
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-05-20
publication
https://doi.org/10.26008/1912/bco-dmo.749774.1
Peter Buston
Boston University
principalInvestigator
Cassidy C. D'Aloia
Boston University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Buston, P., D'Aloia, C. C. (2021) Genotype data for E. lori individuals sequenced at 71 microsatellites from a 41 km-long transect of the Belize Barrier reef during 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-10-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.749774.1 [access date]
Genotype data for E. lori individuals sequenced at 71 microsatellites. Dataset Description: <p>Genotype data for E. lori individuals sequenced at 71 microsatellites.&nbsp;</p> Methods and Sampling: <p>We surveyed a 41 km-long transect of the Belize Barrier reef, centered at Carrie Bow Cay, by SCUBA to conduct a genetic parentage study of the reef fish Elactinus lori. To collect settlers, we sampled ~ 100 individuals every kilometer. Individuals were collected from the outsides of sponges using slurp guns and placed them in plastic bags. At the surface, settlers were anesthetized with MS-222.&nbsp; For genetic analyses, DNA was extracted using a HotSHOT protocol; individuals were sequenced at 71 microsatellite loci using a multiplex PCR protocol for targeted amplicon sequencing. For full details see D’Aloia et al. (2018), Marine Ecology Progress Series.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1260424 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1260424
completed
Peter Buston
Boston University
617-358-5412
Department of Biology 5 Cummington Mall
Boston
MA
02215
USA
buston@bu.edu
pointOfContact
Cassidy C. D'Aloia
Boston University
508-289-2390
Biology Department MS #34
Woods Hole
MA
02543
USA
cassidy.daloia@gmail.com
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sample_ID
site_id
individual_ID
lat
lon
Elor_564_a
Elor_564_b
Elor_2975_a
Elor_2975_b
Elor_3015_a
Elor_3015_b
Elor_605_a
Elor_605_b
Elor_639_a
Elor_639_b
Elor_7629_a
Elor_7629_b
Elor_781_a
Elor_781_b
Elor_8417_a
Elor_8417_b
Elor_4010_a
Elor_4010_b
Elor_3025_a
Elor_3025_b
Elor_7401_a
Elor_7401_b
Elor_476_a
Elor_476_b
Elor_5163_a
Elor_5163_b
Elor_a618_a
Elor_a618_b
Elor_2609_a
Elor_2609_b
Elor_179_a
Elor_179_b
Elor_13691_a
Elor_13691_b
Elor_5115_a
Elor_5115_b
Elor_1592_a
Elor_1592_b
Elor_1698_a
Elor_1698_b
Elor_5782_a
Elor_5782_b
Elor_517_a
Elor_517_b
Elor_1687_a
Elor_1687_b
Elor_6561_a
Elor_6561_b
Elor_483_a
Elor_483_b
Elor_4648_a
Elor_4648_b
Elor_5404_a
Elor_5404_b
Elor_2848_a
Elor_2848_b
Elor_4362_a
Elor_4362_b
Elor_6213_a
Elor_6213_b
Elor_525_a
Elor_525_b
Elor_905_a
Elor_905_b
Elor_25488_a
Elor_25488_b
Elor_1556_a
Elor_1556_b
Elor_3690_a
Elor_3690_b
Elor_5676_a
Elor_5676_b
Elor_6085_a
Elor_6085_b
Elor_6616_a
Elor_6616_b
Elor_13590_a
Elor_13590_b
Elor_1078_a
Elor_1078_b
Elor_5726_a
Elor_5726_b
Elor_7548_a
Elor_7548_b
Elor_6357_a
Elor_6357_b
Elor_976_a
Elor_976_b
Elor_3103_a
Elor_3103_b
Elor_8832_a
Elor_8832_b
Elor_1161_a
Elor_1161_b
Elor_1408_a
Elor_1408_b
Elor_12215_a
Elor_12215_b
Elor_3569_a
Elor_3569_b
Elor_9671_a
Elor_9671_b
Elor_8933_a
Elor_8933_b
Elor_3013_a
Elor_3013_b
Elor_6056_a
Elor_6056_b
Elor_10743_a
Elor_10743_b
Elor_6856_a
Elor_6856_b
Elor_2023_a
Elor_2023_b
Elor_3612_a
Elor_3612_b
Elor_3686_a
Elor_3686_b
Elor_3843_a
Elor_3843_b
Elor_4139_a
Elor_4139_b
Elor_7908_a
Elor_7908_b
Elor_533_a
Elor_533_b
Elor_1079_a
Elor_1079_b
Elor_4622_a
Elor_4622_b
Elor_8200_a
Elor_8200_b
Elor_8924_a
Elor_8924_b
Elor_503_a
Elor_503_b
Goby14528_a
Goby14528_b
Goby21378_a
Goby21378_b
Goby25362_a
Goby25362_b
multiplex PCR protocol
theme
None, User defined
sample identification
site
latitude
longitude
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Thermal Cycler
instrument
BCO-DMO Standard Instruments
Belize_2010
service
Deployment Activity
Carrie Bow Caye, Belizean Barrier Reef (16°48’10’’ N 88°05’45” W)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
An Integrative Investigation of Population Connectivity Using a Coral Reef Fish
http://people.bu.edu/buston/lab/Welcome.html
An Integrative Investigation of Population Connectivity Using a Coral Reef Fish
<p>Understanding the patterns, causes and consequences of larval dispersal is a major goal of 21st century marine ecology. Patterns of dispersal determine the rates of larval exchange, or connectivity, between populations. Both physical factors (e.g., water movement) and biological factors (e.g., larval behavior) cause variation in population connectivity. Population connectivity, in turn, has major consequences for all aspects of an organism's biology, from individual behavior to metapopulation dynamics, and from evolution within metapopulations to the origin and extinction of species. Further, understanding population connectivity is critical for the design of effective networks of marine reserves, creation of vital tools in conservation, and the development of sustainable fisheries.</p>
<p>Over the last decade, three methods, each of which tells something slightly different, have emerged as leading contenders to provide the greatest insights into population connectivity. First, coupled biophysical models make assumptions regarding water flow, larval behavior and ecology, to predict population connectivity. Second, indirect genetic methods use spatial distributions of allele frequencies to infer population connectivity. Third, direct genetic methods use parentage analyses, tracing recruits to specific adults, to measure population connectivity. Despite advances, lack of integration means that we do not know the predictive skill of biophysical models, or the extent to which patterns of dispersal predict spatial genetic structure. The overall objective of this proposal is to conduct an integrated investigation of population connectivity, using all three methods in one tractable system: the neon goby, Elacatinus lori, on the Belizean Barrier Reef. There are three motives for this choice of study system: i) fourteen highly polymorphic microsatellite loci have been developed, facilitating the assignment of recruits to parents using parentage analyses and the measurement of dispersal; ii) the physical oceanography of the Belizean Barrier Reef is well-studied, facilitating the development and testing of coupled biophysical models; and, iii) E. lori has a relatively small biogeographic range, facilitating analysis of the spatial distribution of allele frequencies throughout its range.</p>
<p>Broader Impacts. The grant will support one postdoc and two graduate students who will be trained in scientific diving, marine fieldwork, population genetics, biophysical modeling, and mathematical modeling, and will gain collaborative research experience. PIs will incorporate research findings in their courses, which cover all these topics. The grant will also broaden participation of under-represented groups by supporting six undergraduates from groups traditionally underrepresented in STEM fields. In each year of the project there will be an All Participants meeting to reinforce the network of participants. A project website will be developed, in English and Spanish, on the theme of larval dispersal and population connectivity. This will include a resource for K-12 marine science educators developed in collaboration with a marine science educator. All PIs will ensure that results are broadly disseminated to the scientific community and general public via appropriate forms of media.</p>
Elacatinus Dispersal I
largerWorkCitation
project
eng; USA
oceans
Carrie Bow Caye, Belizean Barrier Reef (16°48’10’’ N 88°05’45” W)
-88.077183
-88.019983
16.625067
16.981
2018-01-01
2018-12-31
Belizean Barrier Reef System (16.803 degrees North 88.096 degrees West)
0
BCO-DMO catalogue of parameters from Genotype data for E. lori individuals sequenced at 71 microsatellites from a 41 km-long transect of the Belize Barrier reef during 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/749782.rdf
Name: sample_ID
Units: unitless
Description: <p>identifier for the sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/749783.rdf
Name: site_id
Units: unitless
Description: <p>identifier for the site</p>
http://lod.bco-dmo.org/id/dataset-parameter/749784.rdf
Name: individual_ID
Units: unitless
Description: <p>identifier for the individual</p>
http://lod.bco-dmo.org/id/dataset-parameter/749785.rdf
Name: lat
Units: decimal degrees
Description: <p>approximate latitude in degrees north</p>
http://lod.bco-dmo.org/id/dataset-parameter/749786.rdf
Name: lon
Units: decimal degrees
Description: <p>approximate longitude in degrees east</p>
http://lod.bco-dmo.org/id/dataset-parameter/749787.rdf
Name: Elor_564_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749788.rdf
Name: Elor_564_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749789.rdf
Name: Elor_2975_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749790.rdf
Name: Elor_2975_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749791.rdf
Name: Elor_3015_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749792.rdf
Name: Elor_3015_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749793.rdf
Name: Elor_605_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749794.rdf
Name: Elor_605_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749795.rdf
Name: Elor_639_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749796.rdf
Name: Elor_639_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749797.rdf
Name: Elor_7629_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749798.rdf
Name: Elor_7629_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749799.rdf
Name: Elor_781_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749800.rdf
Name: Elor_781_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749801.rdf
Name: Elor_8417_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749802.rdf
Name: Elor_8417_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749803.rdf
Name: Elor_4010_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749804.rdf
Name: Elor_4010_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749805.rdf
Name: Elor_3025_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749806.rdf
Name: Elor_3025_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749807.rdf
Name: Elor_7401_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749808.rdf
Name: Elor_7401_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749809.rdf
Name: Elor_476_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749810.rdf
Name: Elor_476_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749811.rdf
Name: Elor_5163_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749812.rdf
Name: Elor_5163_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749813.rdf
Name: Elor_a618_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749814.rdf
Name: Elor_a618_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749815.rdf
Name: Elor_2609_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749816.rdf
Name: Elor_2609_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749817.rdf
Name: Elor_179_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749818.rdf
Name: Elor_179_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749819.rdf
Name: Elor_13691_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749820.rdf
Name: Elor_13691_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749821.rdf
Name: Elor_5115_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749822.rdf
Name: Elor_5115_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749823.rdf
Name: Elor_1592_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749824.rdf
Name: Elor_1592_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749825.rdf
Name: Elor_1698_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749826.rdf
Name: Elor_1698_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749827.rdf
Name: Elor_5782_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749828.rdf
Name: Elor_5782_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749829.rdf
Name: Elor_517_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749830.rdf
Name: Elor_517_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749831.rdf
Name: Elor_1687_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749832.rdf
Name: Elor_1687_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749833.rdf
Name: Elor_6561_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749834.rdf
Name: Elor_6561_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749835.rdf
Name: Elor_483_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749836.rdf
Name: Elor_483_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749837.rdf
Name: Elor_4648_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749838.rdf
Name: Elor_4648_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749839.rdf
Name: Elor_5404_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749840.rdf
Name: Elor_5404_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749841.rdf
Name: Elor_2848_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749842.rdf
Name: Elor_2848_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749843.rdf
Name: Elor_4362_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749844.rdf
Name: Elor_4362_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749845.rdf
Name: Elor_6213_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749846.rdf
Name: Elor_6213_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749847.rdf
Name: Elor_525_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749848.rdf
Name: Elor_525_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749849.rdf
Name: Elor_905_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749850.rdf
Name: Elor_905_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749851.rdf
Name: Elor_25488_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749852.rdf
Name: Elor_25488_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749853.rdf
Name: Elor_1556_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749854.rdf
Name: Elor_1556_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749855.rdf
Name: Elor_3690_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749856.rdf
Name: Elor_3690_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749857.rdf
Name: Elor_5676_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749858.rdf
Name: Elor_5676_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749859.rdf
Name: Elor_6085_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749860.rdf
Name: Elor_6085_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749861.rdf
Name: Elor_6616_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749862.rdf
Name: Elor_6616_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749863.rdf
Name: Elor_13590_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749864.rdf
Name: Elor_13590_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749865.rdf
Name: Elor_1078_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749866.rdf
Name: Elor_1078_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749867.rdf
Name: Elor_5726_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749868.rdf
Name: Elor_5726_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749869.rdf
Name: Elor_7548_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749870.rdf
Name: Elor_7548_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749871.rdf
Name: Elor_6357_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749872.rdf
Name: Elor_6357_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749873.rdf
Name: Elor_976_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749874.rdf
Name: Elor_976_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749875.rdf
Name: Elor_3103_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749876.rdf
Name: Elor_3103_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749877.rdf
Name: Elor_8832_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749878.rdf
Name: Elor_8832_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749879.rdf
Name: Elor_1161_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749880.rdf
Name: Elor_1161_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749881.rdf
Name: Elor_1408_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749882.rdf
Name: Elor_1408_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749883.rdf
Name: Elor_12215_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749884.rdf
Name: Elor_12215_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749885.rdf
Name: Elor_3569_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749886.rdf
Name: Elor_3569_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749887.rdf
Name: Elor_9671_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749888.rdf
Name: Elor_9671_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749889.rdf
Name: Elor_8933_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749890.rdf
Name: Elor_8933_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749891.rdf
Name: Elor_3013_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749892.rdf
Name: Elor_3013_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749893.rdf
Name: Elor_6056_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749894.rdf
Name: Elor_6056_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749895.rdf
Name: Elor_10743_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749896.rdf
Name: Elor_10743_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749897.rdf
Name: Elor_6856_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749898.rdf
Name: Elor_6856_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749899.rdf
Name: Elor_2023_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749900.rdf
Name: Elor_2023_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749901.rdf
Name: Elor_3612_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749902.rdf
Name: Elor_3612_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749903.rdf
Name: Elor_3686_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749904.rdf
Name: Elor_3686_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749905.rdf
Name: Elor_3843_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749906.rdf
Name: Elor_3843_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749907.rdf
Name: Elor_4139_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749908.rdf
Name: Elor_4139_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749909.rdf
Name: Elor_7908_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749910.rdf
Name: Elor_7908_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749911.rdf
Name: Elor_533_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749912.rdf
Name: Elor_533_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749913.rdf
Name: Elor_1079_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749914.rdf
Name: Elor_1079_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749915.rdf
Name: Elor_4622_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749916.rdf
Name: Elor_4622_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749917.rdf
Name: Elor_8200_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749918.rdf
Name: Elor_8200_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749919.rdf
Name: Elor_8924_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749920.rdf
Name: Elor_8924_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749921.rdf
Name: Elor_503_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749922.rdf
Name: Elor_503_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749923.rdf
Name: Goby14528_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749924.rdf
Name: Goby14528_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749925.rdf
Name: Goby21378_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749926.rdf
Name: Goby21378_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749927.rdf
Name: Goby25362_a
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
http://lod.bco-dmo.org/id/dataset-parameter/749928.rdf
Name: Goby25362_b
Units: unitless
Description: <p>field name is the microsatellite locus name. Data is Haplotype id for each microsatellite allele</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1393394
https://darchive.mblwhoilibrary.org/bitstream/1912/27149/1/dataset-749774_gobysibship2018__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.749774.1
download
onLine
dataset
<p>We surveyed a 41 km-long transect of the Belize Barrier reef, centered at Carrie Bow Cay, by SCUBA to conduct a genetic parentage study of the reef fish Elactinus lori. To collect settlers, we sampled ~ 100 individuals every kilometer. Individuals were collected from the outsides of sponges using slurp guns and placed them in plastic bags. At the surface, settlers were anesthetized with MS-222.&nbsp; For genetic analyses, DNA was extracted using a HotSHOT protocol; individuals were sequenced at 71 microsatellite loci using a multiplex PCR protocol for targeted amplicon sequencing. For full details see D’Aloia et al. (2018), Marine Ecology Progress Series.</p>
Specified by the Principal Investigator(s)
<p>Genotype data were analyzed using custom Perl scripts as described in D’Aloia et al. (2016), Conservation Genetics.</p>
<p>BCO-DMO Processing Notes:</p>
<ul>
<li>Every other column of data was for another allele, therefore we&nbsp;appended "_a" to the first and "_b" to the second column.</li>
<li>renamed the individual_ID column to sample_ID</li>
<li>created two additional columns, sample_id and individual_ID,&nbsp;from the sample_ID column by splitting on the underscore.</li>
<li>mapped sample_id value to latitude and longitude coordinates for the sample_ID.</li>
<li>sorted data on sample_ID and individual_ID.</li>
<li>added BCO-DMO conventional header.</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
multiplex PCR protocol
multiplex PCR protocol
PI Supplied Instrument Name: multiplex PCR protocol PI Supplied Instrument Description:individuals were sequenced at 71 microsatellite loci using a multiplex PCR protocol for targeted amplicon sequencing. Instrument Name: Thermal Cycler Instrument Short Name:Thermal Cycler Instrument Description: A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.
(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
Deployment: Belize_2010
Belize_2010
lab Buston
laboratory
Belize_2010
Peter Buston
Boston University
lab Buston
laboratory