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            <gco:CharacterString>Cite this dataset as: Popp, B., Benitez-Nelson, C., Blum, J., Drazen, J., Hannides, C., Seraphin, K. (2018) Amino acid compound specific isotope values for micronekton from R/V Kilo Moana KM1109, KM1123, KM1407, KM1418, and other cruises in the Central North Pacific, Station ALOHA, Tropical Pacific, 2007-2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-12-05 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.750972.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Amino acid specific isotopes in micronekton, station ALOHA Dataset Description: &amp;lt;p&amp;gt;This dataset contains amino acid compound specific concentrations in micronekton collected during R/V Kilo Moana cruises around the ALOHA observatory (KM1407, KM1418, KM2011, and a few other undocumented cruises).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For more information about the ALOHA observatory see: &amp;lt;a href=&amp;quot;http://aco-ssds.soest.hawaii.edu/&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;http://aco-ssds.soest.hawaii.edu/&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;These data were published in Gloeckler et al (2018), Supporting Information file lno10762-sup-0001-suppinfo1.xlsx&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Micronekton were collected using a 10 m2 multiple opening-closing net and environmental sensing system (MOCNESS) at Station ALOHA (22.75˚N, 158˚W) in March and August of 2011 and in February and September of 2014 with a few samples from other locations around Oahu in 2011 (Choy et al 2015). Micronekton were collected over five depth zones between the surface and 1500 m: 0 – 100 m, 100 – 500 m, 500 – 700 m, 700 – 1000 m and 1000 – 1500 m. At sea, micronekton were sorted and identified to the most specific taxonomic level, then measured and photographed. Standard length measurements were taken for fish, carapace length and total length were taken for crustaceans and both mantle length and total length were taken for cephalopods. For most fishes, white muscle tissue was removed and frozen in a cryovial in liquid nitrogen. Small fishes, crustaceans and gelatinous organisms were frozen whole or individuals were pooled for sufficient tissue required for stable isotope analysis. Specimens were transferred to a ‑80˚C freezer until the samples could be prepared for stable isotope analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Eighty-three samples (individual specimens or small groups of conspecifics) were selected for stable isotope analysis. Samples selected for stable isotope analysis represented different combinations of trophic strategies (suspension feeding, zooplanktivores, micronektonivores), depth guilds (epipelagic, mesopelagic, bathypelagic) and migrating behaviors based on available ecological information (e.g., Clarke 1973, Maynard 1982). Each sample was freeze-dried and ground using a ceramic mortar and pestle. For bulk tissue carbon and nitrogen isotope analysis approximately 0.5 mg of each sample was weighed and placed into a tin boat. Carbon and nitrogen isotopic compositions were determined using an isotope ratio mass spectrometer (DeltaPlusXP) coupled to an elemental analyzer (Costech Model 4010). Isotopic ratios are given in δ-notation relative to the international standards VPDB and atmospheric N2. Accuracy and precision were 0.2‰ based on glycine and homogenized fish tissue reference materials analyzed every ten samples. The isotopic compositions of the reference materials have been extensively characterized using NIST certified reference materials in the UH laboratory and verified independently in other isotope laboratories.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Amino acid-specific stable N isotope composition was determined on approximately 15 mg (dry weight) of each sample underwent acid hydrolysis and derivatization yielding trifluoroacetic (TFA) amino acid esters following the methods of Popp et al. (2007) and Hannides et al. (2009b).; The nitrogen isotope composition of the trifluoroacetic amino acid esters were determined using an isotope ratio mass spectrometer (Thermo Scientific Delta V Plus or Thermo Scientific MAT 253 IRMS) interfaced with a Thermo Finnigan GC-C III. Samples were injected onto a BPx5 forte capillary column (60m x 0.32 mm x 1.0 µm film thickness) at an injector temperature of 180˚C with a constant helium flow rate of 1.4 mL/min. The column was initially held at 50˚C for two minutes and then increased at a rate of 15˚C/min to 120˚C. Temperature was then increased at a rate of 4˚C/min to 195˚C, then to 255˚C at a rate of 5˚C/min and finally to 300˚C at a rate of 15˚C/min, holding at the final temperature for eight minutes. Each sample was analyzed in triplicate and co-injected with the reference compounds norleucine (Nor) and aminoadipic acid (AAA) of known isotopic composition. A suite of pure amino acids of known nitrogen isotopic composition (Ala, Thr, Ile, Pro, Glu, and Phe) was also injected every three runs as an extra measure of accuracy for the instrument. Reference compounds Nor and AAA, as well as the suite of amino acids, were used to normalize the measured isotope values. Standard deviation for all amino acids averaged ±0.4‰ (range 0.0-3.1‰).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/560590.rdf" xlink:title="OCE-1433846" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1433846 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1433846</gmx:Anchor>
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Mercury is a pervasive trace element that exists in several states in the marine environment, including monomethylmercury (MMHg), a neurotoxin that bioaccumulates in marine organisms and poses a human health threat. Understanding the fate of mercury in the ocean and resulting impacts on ocean food webs requires understanding the mechanisms controlling the depths at which mercury chemical transformations occur. Preliminary mercury analyses on nine species of marine fish from the North Pacific Ocean indicated that intermediate waters are an important entry point for MMHg into open ocean food webs. To elucidate the process controlling this, researchers will examine mercury dynamics in regions with differing vertical dissolved oxygen profiles, which should influence depths of mercury transformation. Results of the study will aid in a better understanding of the pathways by which mercury enters the marine food chain and can ultimately impact humans. This project will provide training for graduate and undergraduate students, and spread awareness on oceanic mercury through public outreach and informal science programs.&lt;/p&gt;
&lt;p&gt;Mercury isotopic variations can provide insight into a wide variety of environmental processes. Isotopic compositions of mercury display mass-dependent fractionation (MDF) during most biotic and abiotic chemical reactions and mass-independent fractionation (MIF) during photochemical radical pair reactions. The unusual combination of MDF and MIF can provide information on reaction pathways and the biogeochemical history of mercury. Results from preliminary research provide strong evidence that net MMHg formation occurred below the surface mixed layer in the pycnocline and suggested that MMHg in low oxygen intermediate waters is an important entry point for mercury into open ocean food webs. These findings highlight the critical need to understand how MMHg levels in marine biota will respond to changes in atmospheric mercury emissions, deposition of inorganic mercury to the surface ocean, and hypothesized future expansion of oxygen minimum zones. Using field collections across ecosystems with contrasting biogeochemistry and mercury isotope fractionation experiments researchers will fill key knowledge gaps in mercury biogeochemistry. Results of the proposed research will enable scientists to assess the biogeochemical controls on where in the water column mercury methylation and demethylation likely occur.&lt;/p&gt;
&lt;p&gt;&lt;em&gt;Related background publication with supplemental data section:&lt;/em&gt;&lt;br /&gt;
Joel D. Blum, Brian N. Popp, Jeffrey C. Drazen, C. Anela Choy &amp;amp; Marcus W. Johnson. 2013. Methylmercury production below the mixed layer in the North Pacific Ocean. Nature Geoscience 6, 879–884. &lt;a href=&quot;https://www.nature.com/articles/ngeo1918&quot; target=&quot;_blank&quot;&gt;doi:10.1038/ngeo1918&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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	Name: SpeciesID
	Units: unitless
	Description: &lt;p&gt;Genus and species name of micronekton specimen&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751299.rdf
	Name: FamilyID
	Units: unitless
	Description: &lt;p&gt;Taxonomic family of  micronekton specimen&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751300.rdf
	Name: SpecimenID
	Units: unitless
	Description: &lt;p&gt;specimen identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751301.rdf
	Name: Sample_site
	Units: unitless
	Description: &lt;p&gt;specimen collection site&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751302.rdf
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	Units: unitless
	Description: &lt;p&gt;year of collection&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751303.rdf
	Name: Month
	Units: unitless
	Description: &lt;p&gt;month of collection&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751304.rdf
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	Units: unitless
	Description: &lt;p&gt;cruise identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751305.rdf
	Name: n
	Units: individuals
	Description: &lt;p&gt;number of specimens in sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751306.rdf
	Name: Length_mm
	Units: millimeters
	Description: &lt;p&gt;length of specimen&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751307.rdf
	Name: Length_Type
	Units: length
	Description: &lt;p&gt;Length type is standard length (SL); total length (TL); carapace length (CL); fork length (FL); mantle length (ML)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751308.rdf
	Name: Tissue_Type
	Units: sample_type
	Description: &lt;p&gt;Type of tissue samples taken: WMT = white muscle tissue; Whole = the whole specimen;&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751309.rdf
	Name: delta15N_ppt_v_AIR
	Units: permil
	Description: &lt;p&gt;ratio of tissue 15N:14N isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751310.rdf
	Name: delta13C_ppt_v_VPDB
	Units: permil
	Description: &lt;p&gt;ratio of tissue 13C:12C isotope relative to VPDB (Vienna Pee Dee Belemnite)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751311.rdf
	Name: C_N_mol_mol
	Units: unitless
	Description: &lt;p&gt;Carbon to Nitrogen ratio&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751510.rdf
	Name: Alanine
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Alanine isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751511.rdf
	Name: Glycine
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Glycine isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751512.rdf
	Name: Threonine
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Threonine isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751513.rdf
	Name: Serine
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Serine isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751514.rdf
	Name: Valine
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Valine isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751515.rdf
	Name: Leucine
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Leucine isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751516.rdf
	Name: Isoleucine
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Isoleucine isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751517.rdf
	Name: Proline
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Proline isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751518.rdf
	Name: Aspartic_acid
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Aspartic_acid isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751519.rdf
	Name: Methionine
	Units: unitless
	Description: &lt;p&gt;ratio of tissue Methionine isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751520.rdf
	Name: Glutamic_acid
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	Description: &lt;p&gt;ratio of tissue Glutamic_acid isotopes relative to atmospheric N2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/751521.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/751522.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/751523.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/751524.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/751525.rdf
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&amp;lt;p&amp;gt;Eighty-three samples (individual specimens or small groups of conspecifics) were selected for stable isotope analysis. Samples selected for stable isotope analysis represented different combinations of trophic strategies (suspension feeding, zooplanktivores, micronektonivores), depth guilds (epipelagic, mesopelagic, bathypelagic) and migrating behaviors based on available ecological information (e.g., Clarke 1973, Maynard 1982). Each sample was freeze-dried and ground using a ceramic mortar and pestle. For bulk tissue carbon and nitrogen isotope analysis approximately 0.5 mg of each sample was weighed and placed into a tin boat. Carbon and nitrogen isotopic compositions were determined using an isotope ratio mass spectrometer (DeltaPlusXP) coupled to an elemental analyzer (Costech Model 4010). Isotopic ratios are given in δ-notation relative to the international standards VPDB and atmospheric N2. Accuracy and precision were 0.2‰ based on glycine and homogenized fish tissue reference materials analyzed every ten samples. The isotopic compositions of the reference materials have been extensively characterized using NIST certified reference materials in the UH laboratory and verified independently in other isotope laboratories.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Amino acid-specific stable N isotope composition was determined on approximately 15 mg (dry weight) of each sample underwent acid hydrolysis and derivatization yielding trifluoroacetic (TFA) amino acid esters following the methods of Popp et al. (2007) and Hannides et al. (2009b).; The nitrogen isotope composition of the trifluoroacetic amino acid esters were determined using an isotope ratio mass spectrometer (Thermo Scientific Delta V Plus or Thermo Scientific MAT 253 IRMS) interfaced with a Thermo Finnigan GC-C III. Samples were injected onto a BPx5 forte capillary column (60m x 0.32 mm x 1.0 µm film thickness) at an injector temperature of 180˚C with a constant helium flow rate of 1.4 mL/min. The column was initially held at 50˚C for two minutes and then increased at a rate of 15˚C/min to 120˚C. Temperature was then increased at a rate of 4˚C/min to 195˚C, then to 255˚C at a rate of 5˚C/min and finally to 300˚C at a rate of 15˚C/min, holding at the final temperature for eight minutes. Each sample was analyzed in triplicate and co-injected with the reference compounds norleucine (Nor) and aminoadipic acid (AAA) of known isotopic composition. A suite of pure amino acids of known nitrogen isotopic composition (Ala, Thr, Ile, Pro, Glu, and Phe) was also injected every three runs as an extra measure of accuracy for the instrument. Reference compounds Nor and AAA, as well as the suite of amino acids, were used to normalize the measured isotope values. Standard deviation for all amino acids averaged ±0.4‰ (range 0.0-3.1‰).&amp;lt;/p&amp;gt;</gco:CharacterString>
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