http://lod.bco-dmo.org/id/dataset/751313
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-12-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Amino acid compound specific isotope values for particles from R/V Kilo Moana KM1407 and KM1418 in the Central North Pacific, Station ALOHA, Tropical Pacific, Feb and Sept. 2014
2018-12-05
publication
2018-12-05
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-18
publication
https://doi.org/10.1575/1912/bco-dmo.751313.1
Brian N. Popp
University of Hawaiʻi at Mānoa
principalInvestigator
Claudia R. Benitez-Nelson
University of South Carolina at Columbia
principalInvestigator
Joel D. Blum
University of Michigan
principalInvestigator
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
principalInvestigator
Cecelia Hannides
University of Hawaiʻi at Mānoa
principalInvestigator
Kanesa Seraphin
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Popp, B., Benitez-Nelson, C., Blum, J., Drazen, J., Hannides, C., Seraphin, K. (2018) Amino acid compound specific isotope values for particles from R/V Kilo Moana KM1407 and KM1418 in the Central North Pacific, Station ALOHA, Tropical Pacific, Feb and Sept. 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-12-05 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.751313.1 [access date]
Amino acid specific isotopes in particles, station ALOHA Dataset Description: <p>This dataset contains amino acid compound specific nitrogen isotope ratios in particles collected during R/V Kilo Moana cruises around Station ALOHA (KM1407 and KM1418).</p>
<p>For more information about the ALOHA observatory see: <a href="http://aco-ssds.soest.hawaii.edu/" target="_blank">http://aco-ssds.soest.hawaii.edu/</a></p>
<p>These data were published in Gloeckler et al (2018), Supporting Information file lno10762-sup-0002-suppinfo2.xlsx</p> Methods and Sampling: <p>Amino acid-specific stable N isotope composition was determined on samples that were hydrolyzed, derivatized, and analyzed according to Popp et al. (2007) and Hannides et al. (2009).&nbsp; Briefly, size-fractioned zooplankton material and target zooplankton taxa were hydrolyzed using trace metal-grade 6 M HCl and the resulting AAs purified using cation exchange chromatography.&nbsp; The samples were then esterified using 4:1 isopropanol:acetyl chloride and derivatized using 3:1 methylene chloride:trifluoroacetyl anhydride.&nbsp; The resulting trifluoroacetyl and isopropyl ester (TFA) derivatives were purified using chloroform extraction and stored at -20°C for up to 1 month before analysis.&nbsp; This method yielded information for the following AAs: alanine (Ala), glycine (Gly), isoleucine (Ile), leucine (Leu), lysine (Lys), methionine (Met), phenylalanine (Phe), proline (Pro), serine (Ser), threonine (Thr), tyrosine (Tyr), and valine (Val).&nbsp; During acid hydrolysis asparagine (Asn) is converted to aspartic acid (Asp) and glutamine (Gln) is converted to glutamic acid (Glu), thus we also report information on the combined pools, termed Asx (Asn+Asp) and Glx (Gln+Glu), respectively.</p>
<p>TFA derivatives of AAs were analyzed for stable N isotope composition (d15NAA values) following Hannides et al. (2013).&nbsp; AAs were analyzed using a Thermo Scientific Delta V Plus IRMS interfaced to a trace gas chromatograph (GC) fitted with a 60 m BPx5 capillary column through a GC-C III combustion furnace (980°C), reduction furnace (680°C) and liquid nitrogen cold trap. d15NAA values were measured on 3 – 5 replicate injections with norleucine and aminoadipic acid with known d15N values as internal reference materials co-injected on each run.</p>
<p>A composite source d15NAA value was calculated by a weighted averaging a suite of AAs (e.g., d15NSrc-AA = average of Gly, Lys, Phe, and Ser d15N values). Weighting was based the analytical uncertainty calculated from at least triplicate analysis of each sample.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1433846 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1433846
completed
Brian N. Popp
University of Hawaiʻi at Mānoa
808-956-6206
Department of Earth Sciences 1680 East-West Road
Honolulu
HI
96822
USA
popp@hawaii.edu
pointOfContact
Claudia R. Benitez-Nelson
University of South Carolina at Columbia
803-777-0525
School of the Earth, Ocean and Environment 701 Sumter Street
Columbia
SC
29208
USA
benitezn@mailbox.sc.edu
pointOfContact
Joel D. Blum
University of Michigan
734-615-3242
1100 North University Ave
Ann Arbor
MI
48109
USA
jdblum@umich.edu
pointOfContact
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
808-956-6567
Department of Oceanography, SOEST 1000 Pope Road
Honolulu
HI
96822
USA
jdrazen@hawaii.edu
pointOfContact
Cecelia Hannides
University of Hawaiʻi at Mānoa
Department of Geology and Geophysics and Department of Oceanography 1680 East-West Road
Honolulu
HI
96822
USA
ceceliah@hawaii.edu
pointOfContact
Kanesa Seraphin
University of Hawaiʻi at Mānoa
808-225-1753
UH Sea Grant Center for Marine Science Education, University of Hawaii 2525 Correa Road
Honolulu
HI
96822
USA
kanesa@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Particle_size
Sample
Depth
delta15N
delta15N_stdev
comment
Thermo Scientific Delta V Plus IRMS
theme
None, User defined
diameter
sample identification
depth
d15N
comments
featureType
BCO-DMO Standard Parameters
Gas Chromatograph
Ion Chromatograph
McLane Large Volume Pumping System WTS-LV
instrument
BCO-DMO Standard Instruments
KM1407
KM1418
service
Deployment Activity
Central North Pacific, Station ALOHA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Isotopic insights to mercury in marine food webs and how it varies with ocean biogeochemistry
https://osprey.bco-dmo.org/project/560580
Collaborative Research: Isotopic insights to mercury in marine food webs and how it varies with ocean biogeochemistry
<p><em>NSF award abstract:</em><br />
Mercury is a pervasive trace element that exists in several states in the marine environment, including monomethylmercury (MMHg), a neurotoxin that bioaccumulates in marine organisms and poses a human health threat. Understanding the fate of mercury in the ocean and resulting impacts on ocean food webs requires understanding the mechanisms controlling the depths at which mercury chemical transformations occur. Preliminary mercury analyses on nine species of marine fish from the North Pacific Ocean indicated that intermediate waters are an important entry point for MMHg into open ocean food webs. To elucidate the process controlling this, researchers will examine mercury dynamics in regions with differing vertical dissolved oxygen profiles, which should influence depths of mercury transformation. Results of the study will aid in a better understanding of the pathways by which mercury enters the marine food chain and can ultimately impact humans. This project will provide training for graduate and undergraduate students, and spread awareness on oceanic mercury through public outreach and informal science programs.</p>
<p>Mercury isotopic variations can provide insight into a wide variety of environmental processes. Isotopic compositions of mercury display mass-dependent fractionation (MDF) during most biotic and abiotic chemical reactions and mass-independent fractionation (MIF) during photochemical radical pair reactions. The unusual combination of MDF and MIF can provide information on reaction pathways and the biogeochemical history of mercury. Results from preliminary research provide strong evidence that net MMHg formation occurred below the surface mixed layer in the pycnocline and suggested that MMHg in low oxygen intermediate waters is an important entry point for mercury into open ocean food webs. These findings highlight the critical need to understand how MMHg levels in marine biota will respond to changes in atmospheric mercury emissions, deposition of inorganic mercury to the surface ocean, and hypothesized future expansion of oxygen minimum zones. Using field collections across ecosystems with contrasting biogeochemistry and mercury isotope fractionation experiments researchers will fill key knowledge gaps in mercury biogeochemistry. Results of the proposed research will enable scientists to assess the biogeochemical controls on where in the water column mercury methylation and demethylation likely occur.</p>
<p><em>Related background publication with supplemental data section:</em><br />
Joel D. Blum, Brian N. Popp, Jeffrey C. Drazen, C. Anela Choy & Marcus W. Johnson. 2013. Methylmercury production below the mixed layer in the North Pacific Ocean. Nature Geoscience 6, 879–884. <a href="https://www.nature.com/articles/ngeo1918" target="_blank">doi:10.1038/ngeo1918</a></p>
Hg_Biogeochemistry
largerWorkCitation
project
eng; USA
oceans
Central North Pacific, Station ALOHA
-158
-158
22.75
22.75
2014-02-19
2014-09-11
Pacific Subtropical Gyre, Station ALOHA 22.75N 158W; equatorial Pacific (10N 155W, 5N 155W)
0
BCO-DMO catalogue of parameters from Amino acid compound specific isotope values for particles from R/V Kilo Moana KM1407 and KM1418 in the Central North Pacific, Station ALOHA, Tropical Pacific, Feb and Sept. 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/751351.rdf
Name: Particle_size
Units: microns
Description: <p>particle size</p>
http://lod.bco-dmo.org/id/dataset-parameter/751352.rdf
Name: Sample
Units: unitless
Description: <p>sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/751353.rdf
Name: Depth
Units: meters
Description: <p>sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/751354.rdf
Name: delta15N
Units: permil
Description: <p>ratio of tissue 15N:14N isotopes</p>
http://lod.bco-dmo.org/id/dataset-parameter/751355.rdf
Name: delta15N_stdev
Units: permil
Description: <p>standard deviation of ratio of tissue 15N:14N isotopes</p>
http://lod.bco-dmo.org/id/dataset-parameter/751356.rdf
Name: comment
Units: unitless
Description: <p>comments</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
694
https://darchive.mblwhoilibrary.org/bitstream/1912/23845/1/dataset-751313_particle-aa-csia-aloha-amino-acid-specific-isotopes-particles__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.751313.1
download
onLine
dataset
<p>Amino acid-specific stable N isotope composition was determined on samples that were hydrolyzed, derivatized, and analyzed according to Popp et al. (2007) and Hannides et al. (2009).&nbsp; Briefly, size-fractioned zooplankton material and target zooplankton taxa were hydrolyzed using trace metal-grade 6 M HCl and the resulting AAs purified using cation exchange chromatography.&nbsp; The samples were then esterified using 4:1 isopropanol:acetyl chloride and derivatized using 3:1 methylene chloride:trifluoroacetyl anhydride.&nbsp; The resulting trifluoroacetyl and isopropyl ester (TFA) derivatives were purified using chloroform extraction and stored at -20°C for up to 1 month before analysis.&nbsp; This method yielded information for the following AAs: alanine (Ala), glycine (Gly), isoleucine (Ile), leucine (Leu), lysine (Lys), methionine (Met), phenylalanine (Phe), proline (Pro), serine (Ser), threonine (Thr), tyrosine (Tyr), and valine (Val).&nbsp; During acid hydrolysis asparagine (Asn) is converted to aspartic acid (Asp) and glutamine (Gln) is converted to glutamic acid (Glu), thus we also report information on the combined pools, termed Asx (Asn+Asp) and Glx (Gln+Glu), respectively.</p>
<p>TFA derivatives of AAs were analyzed for stable N isotope composition (d15NAA values) following Hannides et al. (2013).&nbsp; AAs were analyzed using a Thermo Scientific Delta V Plus IRMS interfaced to a trace gas chromatograph (GC) fitted with a 60 m BPx5 capillary column through a GC-C III combustion furnace (980°C), reduction furnace (680°C) and liquid nitrogen cold trap. d15NAA values were measured on 3 – 5 replicate injections with norleucine and aminoadipic acid with known d15N values as internal reference materials co-injected on each run.</p>
<p>A composite source d15NAA value was calculated by a weighted averaging a suite of AAs (e.g., d15NSrc-AA = average of Gly, Lys, Phe, and Ser d15N values). Weighting was based the analytical uncertainty calculated from at least triplicate analysis of each sample.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing Notes:<br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- added comment column</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Thermo Scientific Delta V Plus IRMS
Thermo Scientific Delta V Plus IRMS
PI Supplied Instrument Name: Thermo Scientific Delta V Plus IRMS PI Supplied Instrument Description:Used to analyze amino acids. Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
PI Supplied Instrument Name: PI Supplied Instrument Description:Amino acids were purified using cation exchange chromatography. Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)
PI Supplied Instrument Name: PI Supplied Instrument Description:Particles were collected using in situ filtration. Instrument Name: McLane Large Volume Pumping System WTS-LV Instrument Short Name:WTS-LV Instrument Description: The WTS-LV is a Water Transfer System (WTS) Large Volume (LV) pumping instrument designed and manufactured by McLane Research Labs (Falmouth, MA, USA). It is a large-volume, single-event sampler that collects suspended and dissolved particulate samples in situ.
Ambient water is drawn through a modular filter holder onto a 142-millimeter (mm) membrane without passing through the pump. The standard two-tier filter holder provides prefiltering and size fractioning. Collection targets include chlorophyll maximum, particulate trace metals, and phytoplankton. It features different flow rates and filter porosity to support a range of specimen collection. Sampling can be programmed to start at a scheduled time or begin with a countdown delay. It also features a dynamic pump speed algorithm that adjusts flow to protect the sample as material accumulates on the filter. Several pump options range from 0.5 to 30 liters per minute, with a max volume of 2,500 to 36,000 liters depending on the pump and battery pack used. The standard model is depth rated to 5,500 meters, with a deeper 7,000-meter option available. The operating temperature is -4 to 35 degrees Celsius.
The WTS-LV is available in four different configurations: Standard, Upright, Bore Hole, and Dual Filter Sampler. The high-capacity upright WTS-LV model provides three times the battery life of the standard model. The Bore-Hole WTS-LV is designed to fit through a narrow opening such as a 30-centimeter borehole. The dual filter WTS-LV features two vertical intake 142 mm filter holders to allow simultaneous filtering using two different porosities.
Cruise: KM1407
KM1407
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1407
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
Cruise: KM1418
KM1418
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1418
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel