<div><p>Two 10 cm fragments (1 for control tank and 1 for treatment tank) from five colonies of each acroporid taxon were collected from Flat Cay the day prior to the experiment and left under ambient temperature conditions overnight. Tissue samples (2cm) were collected immediately prior to and immediately after the experiment and preserved in a Z-Fix Concentrate: seawater (1:4) solution. One fragment (putative genotype) from each taxon was placed in a control tank (n=5) and one was placed in a treatment tanks (n=5). Powerheads that were slightly tilted out of the water to provide aeration and water movement were placed in all tanks. The ambient tanks were placed in a large tank with constant flowing seawater that served as a water bath. The five treatment tanks, each with an individual heater, were place in a neighboring large tank with no running seawater. All tanks were under a covered porch, but exposed to ambient light conditions. </p>
<p>A Hobo temperature logger was placed in each tank and a water temperature measurement was collected every 30 seconds. The control tanks ranged from 27.5 to 29.4C with an average of 28.2C and the heated tanks ranged from 29.1 to 32.7C with an average of 31.3C over the 48 hour experimental period. </p></div>
Histological analysis of Caribbean acroporid corals before and after exposure to thermal stress.
<div><p>A pilot project was conducted in the St. Thomas, U.S. Virgin Islands to examine the effects of thermal stress on the three acroporid taxon. </p></div>
Coral histology
<div><p>The semi-quantitative rubric was designed by Dr. Esther Peters for the histological analysis. </p>
<p>Data legend:<br />
ND = No Data<br />
NS = not enough tissue to determine, not seen</p>
<p>Lesion Description: <br />
H = healthy<br />
WBD = white band disease<br />
RTL = rapid tissue loss<br />
BL = bleaching</p>
<p>Bleaching: <br />
0 = none<br />
1 = paling<br />
2 = bleaching</p>
<p>Bleaching Distribution:<br />
1 = focal<br />
2 = multifocal distinct<br />
3 = multifocal indistinct<br />
4 = diffuse</p>
<p>Condition Scores: <br />
0 = excellent<br />
1 = very good<br />
2 = good<br />
3 = fair<br />
4 = poor<br />
5 = very poor</p>
<p>Intensity/Severity Scores:<br />
0 = no change<br />
1 = minimal<br />
2 = mild<br />
3 = moderate<br />
4 = marked<br />
5 = severe</p>
<p>Other:<br />
P = present<br />
A = absent<br />
SI = staining issue<br />
NS = not seen<br />
N/A = not available<br />
N = no</p>
<p>Epidermal mucocytes:<br />
0 = not larger than ciliated columnar cells, uniform distribution<br />
1 = slightly hypertrophied, numerous<br />
2 = many hypertophied, abundant mucus release<br />
3 = uneven, hypertrophied vs reduced <br />
4 = atrophy, necrosis, and epidermal foci lacking mucocytes<br />
5 = atrophy and most mucocytes lacking</p>
<p>Mesenterial Filament (CGB Epithelium) Mucocytes:<br />
0 = none to few<br />
1 = less than 1/2 area, not hypertrophied<br />
2 = about 1/2 area, some hypertrophied<br />
3 = about 1/2 area, all hypertrophied <br />
4 = about 3/4/ area, some vacuolated<br />
5 = much of area vacuolated, necrotic, loss of mucocytes</p>
<p>Calicodermis Condition:<br />
0 = cells squamous but "thick," cover mesoglea<br />
1 = cells slightly atrophied in some foci<br />
2 = 1/2 of cells atrophied<br />
3 = Most cells atrophied, beginning separation from mesoglea<br />
4 = Cells atrophied and loss or sloughing present</p>
<p>Costal Tissue Loss:<br />
0 = none, tissue over costae intact<br />
1 = a few gaps noted<br />
2 = 1/4 of costae exposed<br />
3 = 1/2 of costae exposed<br />
4 = 3/4 of costae exposed<br />
5 = Most costae exposed, tissues attenuated, retracted?</p>
<p>Oocytes:<br />
0 - none present<br />
1 - single cells in mesoglea<br />
2 - early oocytes<br />
3 - mid-development<br />
4 - mature<br />
5 - spawned</p>
<p>Spermaries:<br />
0 - none present<br />
1 - clusters of single cells in mesoglea<br />
2 - few, early spermaries<br />
3 - numerous spermaries, spermatocytes<br />
4 - mature, spermatozoa<br />
5 - spawne</p>
<p>BCO-DMO Processing Notes:</p>
<ul><li>added conventional header with dataset name, PI name, version date</li>
<li>modified parameter names to conform with BCO-DMO naming conventions</li>
<li>added lat, lon columns</li>
</ul></div>
752550
Coral histology
2019-01-08T10:44:27-05:00
2019-01-08T10:44:27-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:752550
Histological analysis of Caribbean acroporid corals collected in September 2014 before and after exposure to thermal stress in St. Thomas, U.S. Virgin Islands.
Histological analysis of Caribbean acroporid corals collected in September 2014 before and after exposure to thermal stress in St. Thomas, U.S. Virgin Islands.
false
Fogarty, N. (2021) Histological analysis of Caribbean acroporid corals collected in September 2014 before and after exposure to thermal stress in St. Thomas, U.S. Virgin Islands. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-01-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.752550.1 [access date]
true
1
10.26008/1912/bco-dmo.752550.1
false
2019-01-08
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