http://lod.bco-dmo.org/id/dataset/752550
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-01-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Histological analysis of Caribbean acroporid corals collected in September 2014 before and after exposure to thermal stress in St. Thomas, U.S. Virgin Islands.
2019-01-08
publication
2019-01-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-01-20
publication
https://doi.org/10.26008/1912/bco-dmo.752550.1
Nicole Fogarty
Nova Southeastern University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fogarty, N. (2021) Histological analysis of Caribbean acroporid corals collected in September 2014 before and after exposure to thermal stress in St. Thomas, U.S. Virgin Islands. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-01-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.752550.1 [access date]
Histological analysis of Caribbean acroporid corals before and after exposure to thermal stress. Dataset Description: <p>A pilot project was conducted in the St. Thomas, U.S. Virgin Islands to examine the effects of thermal stress on the three acroporid taxon.&nbsp;</p> Methods and Sampling: <p>Two 10 cm fragments (1 for control tank and 1 for treatment tank) from five colonies of each acroporid taxon were collected from Flat Cay the day prior to the experiment and left under ambient temperature conditions overnight. Tissue samples (2cm) were collected immediately prior to and immediately after the experiment and preserved in a Z-Fix Concentrate: seawater (1:4) solution. One fragment (putative genotype) from each taxon was placed in a control tank (n=5) and one was placed in a treatment tanks (n=5).&nbsp; Powerheads that were slightly tilted out of the water to provide aeration and water movement were placed in all tanks. The ambient tanks were placed in a large tank with constant flowing seawater that served as a water bath. The five treatment tanks, each with an individual heater, were place in a neighboring large tank with no running seawater. All tanks were under a covered porch, but exposed to ambient light conditions.&nbsp;</p>
<p>A Hobo temperature logger was placed in each tank and a water temperature measurement was collected every 30 seconds. The control tanks ranged from 27.5 to 29.4C with an average of 28.2C and the heated tanks ranged from 29.1 to 32.7C with an average of 31.3C over the 48 hour experimental period.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538469 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538469
completed
Nicole Fogarty
Nova Southeastern University
910-962-3397
5600 Marvin K. Moss Ln
Wilmington
NC
28409
USA
fogartyn@uncw.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Lab
Histo_Number
Study
Collector
Species
Sample_Location
Field_or_Lab_Number
Date_Collected
Sample_Type
Lesion_Description
Bleaching
Colony_Bleaching_Distribution
Tissue_Loss
In_situ_Photo
Gross_Photo_Fixed_Sample
Collection_Notes
Sample_Number
Piece_Number
Number_of_Blocks_Made
Fixation
Enrobing
Decal
Embed
Number_of_Slides_Made
Number_Stained_with_H_E
Other_Stains
General_Condition_10x
Zooxanthellae_10x
Epidermal_Mucocytes_Condition
Mesenterial_Filament_Mucocytes
Degeneration_Cnidoglandular_Bands
Dissociation_of_Mesenterial_Filaments
Calicodermis_Condition
Calicodermis_Repair
Costal_Tissue_Loss
Necrotic_Cell_Spherules
WBD_Bacterial_Aggregates
Epidermal_RLOs
Filament_RLOs
Gastrodermal_RLOs
Surface_Bacteria
Coccidia
Zooxanthellate_Ciliates
Non_zooxanthellate_Ciliates
Oocytes_Stage_in_Development
Spermaries_Stage_in_Development
lat
lon
Hobo Pro v2 data logger
theme
None, User defined
responsible_entity
sample identification
sample description
person name
species scientific name (binomial)
site description
site
date
sample type
No BCO-DMO term
count
latitude
longitude
featureType
BCO-DMO Standard Parameters
Temperature Logger
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative research: Is hybridization among threatened Caribbean coral species the key to their survival or the harbinger of their extinction?
https://osprey.bco-dmo.org/project/663794
Collaborative research: Is hybridization among threatened Caribbean coral species the key to their survival or the harbinger of their extinction?
<p><em>NSF Award Abstract:</em><br />
Reef-building acroporid corals form the foundation of shallow tropical coral communities throughout the Caribbean. Yet, the once dominant staghorn coral (Acropora cervicornis) and the elkhorn coral (A. palmata) have decreased by more than 90% since the 1980s, primarily from disease. Their continuing decline jeopardizes the ability of coral reefs to provide numerous societal and ecological benefits, including economic revenue from seafood harvesting and tourism and shoreline protection from extreme wave events caused by storms and hurricanes. Despite their protection under the U.S. Endangered Species Act since 2006, threats to the survival of reef-building acroporid corals remain pervasive and include disease and warming ocean temperatures that may lead to further large-scale mortality. However, hybridization among these closely related species is increasing and may provide an avenue for adaptation to a changing environment. While hybrids were rare in the past, they are now thriving in shallow habitats with extreme temperatures and irradiance and are expanding into the parental species habitats. Additional evidence suggests that the hybrid is more disease resistant than at least one of the parental species. Hybridization may therefore have the potential to rescue the threatened parental species from extinction through the transfer of adapted genes via hybrids mating with both parental species, but extensive gene flow may alter the evolutionary trajectory of the parental species and drive one or both to extinction. This collaborative project is to collect genetic and ecological data in order to understand the mechanisms underlying increasing hybrid abundance. The knowledge gained from this research will help facilitate more strategic management of coral populations under current and emerging threats to their survival. This project includes integrated research and educational opportunities for high school, undergraduate and graduate students, and a postdoctoral researcher. Students in the United States Virgin Islands will take part in coral spawning research and resource managers will receive training on acroporid reproduction to apply to coral restoration techniques.</p>
<p>Current models predict the demise of reefs in the next 200 years due to increasing sea surface temperatures and ocean acidification. It is thus essential to identify habitats, taxa and evolutionary mechanisms that will allow some coral species to maintain their role as foundation fauna. Hybridization can provide an avenue for adaptation to changing conditions. Corals hybridize with some frequency and results may range from the introduction of a few alleles into existing parent species via introgression, to the birth of a new, perhaps better adapted genetic lineage. The only widely accepted coral hybrid system consists of the once dominant but now threatened Caribbean species, Acropora cervicornis and A. palmata. In the past, hybrid colonies originating from natural crosses between elkhorn and staghorn corals were rare, and evidence of hybrid reproduction was limited to infrequent matings with the staghorn coral. Recent field observations suggest that the hybrid is increasing and its ecological role is changing throughout the Caribbean. These hybrids appear to be less affected by the disease that led to the mass mortality of their parental species in recent decades. Hybrids are also found thriving in shallow habitats with high temperatures and irradiance suggesting they may be less susceptible to future warming scenarios. At the same time, they are expanding into the deeper parental species habitats. Preliminary genetic data indicate that hybrids are now mating with each other, demonstrating the potential for the formation of a new species. Further, hybrids appear to be capable of mating with both staghorn and elkhorn coral, perhaps leading to gene flow between the parent species via the hybrid. Research is proposed to address how the increase in hybridization and perhaps subsequent introgression will affect the current ecological role and the future evolutionary trajectory of Caribbean acroporids. Specifically, this collaborative project aims to answer the following questions: 1) What is the historic rate, direction, and degree of introgression across species ranges and genomes? Linkage block analysis based on genome-wide SNP genotyping across three replicate hybrid zones will answer this question. 2) What is the current extent and future potential of later generation hybrid formation? Morphometric and genetic analyses combined with in vitro fertilization assays will be used. 3) What mechanisms allow hybrids to thrive in hot, shallow waters? A series of manipulative in situ and ex situ experiments will determine whether biotic or abiotic factors favor hybrid survival in shallow waters. 4) Are hybrids more disease resistant than the parentals species? Disease transmission assays in reciprocal transplant experiments and histological analysis to determine the extent of disease will be conducted. A multidisciplinary approach will be taken that combines traditional and cutting edge technology to provide a detailed analysis of the evolutionary ecology of Caribbean corals.</p>
<p><em>Note: </em>PI Nicole Fogarty's original award OCE-1538469 was issued while at Nova Southeastern University. This was replaced by OCE-1929979 upon moving to the University of North Carolina Wilmington.</p>
Coral Hybridization
largerWorkCitation
project
eng; USA
oceans
-64.98923
-64.98923
18.31701
18.31701
2014-09-20
2014-09-22
Caribbean and North-West Atlantic
0
BCO-DMO catalogue of parameters from Histological analysis of Caribbean acroporid corals collected in September 2014 before and after exposure to thermal stress in St. Thomas, U.S. Virgin Islands.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/752558.rdf
Name: Lab
Units: unitless
Description: <p>Academic affiliation</p>
http://lod.bco-dmo.org/id/dataset-parameter/752559.rdf
Name: Histo_Number
Units: unitless
Description: <p>Sample number</p>
http://lod.bco-dmo.org/id/dataset-parameter/752560.rdf
Name: Study
Units: unitless
Description: <p>Study title</p>
http://lod.bco-dmo.org/id/dataset-parameter/752561.rdf
Name: Collector
Units: unitless
Description: <p>PI on project</p>
http://lod.bco-dmo.org/id/dataset-parameter/752562.rdf
Name: Species
Units: unitless
Description: <p>A.cerv = Acropora cervicornis; A.pal = Acropora palmata; A. pro = Acropora prolifera</p>
http://lod.bco-dmo.org/id/dataset-parameter/752563.rdf
Name: Sample_Location
Units: unitless
Description: <p>Collection location (18.31701 -64.98923)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752564.rdf
Name: Field_or_Lab_Number
Units: unitless
Description: <p>assigned coral identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/752565.rdf
Name: Date_Collected
Units: unitless
Description: <p>coral collection date in MM/DD/YY format</p>
http://lod.bco-dmo.org/id/dataset-parameter/752566.rdf
Name: Sample_Type
Units: unitless
Description: <p>location within coral colony</p>
http://lod.bco-dmo.org/id/dataset-parameter/752567.rdf
Name: Lesion_Description
Units: unitless
Description: <p>Healthy (apparently) (H); White band disease (WBD); Rapid tissue loss (RTL); Bleaching (BL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752568.rdf
Name: Bleaching
Units: unitless
Description: <p>0 = No; 1 = Paling; 2 = Bleaching</p>
http://lod.bco-dmo.org/id/dataset-parameter/752569.rdf
Name: Colony_Bleaching_Distribution
Units: unitless
Description: <p>1 = Focal; 2 = Multifocal Distinct; 3 = Multifocal Indistinct; 4 = Diffuse</p>
http://lod.bco-dmo.org/id/dataset-parameter/752570.rdf
Name: Tissue_Loss
Units: unitless
Description: <p>Is the skeleton void of tissue? (Y or N)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752571.rdf
Name: In_situ_Photo
Units: unitless
Description: <p>Was a photograph taken during the experiment? (Y or N)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752572.rdf
Name: Gross_Photo_Fixed_Sample
Units: unitless
Description: <p>Was a photograph taken after the sample was preserved? (Y or N)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752573.rdf
Name: Collection_Notes
Units: unitless
Description: <p>anything notable about photographs</p>
http://lod.bco-dmo.org/id/dataset-parameter/752574.rdf
Name: Sample_Number
Units: unitless
Description: <p>The assigned unique identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/752575.rdf
Name: Piece_Number
Units: unitless
Description: <p>Pre= collected before experiment; Post= collected after experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/752576.rdf
Name: Number_of_Blocks_Made
Units: unitless
Description: <p>The number of samples embedded in paraffin to be sectioned and mounted on slides</p>
http://lod.bco-dmo.org/id/dataset-parameter/752577.rdf
Name: Fixation
Units: unitless
Description: <p>preservative used (Zfix:seawater in 1:4)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752578.rdf
Name: Enrobing
Units: unitless
Description: <p>Was the sample enrobbed in agarose gel to preserve the oritentation of cells and tissues while processing and embedding the sample? (Typically required if there is tissue sloughing on the sample)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752579.rdf
Name: Decal
Units: unitless
Description: <p>The solution used to decalcifiy the coral skeleton</p>
http://lod.bco-dmo.org/id/dataset-parameter/752580.rdf
Name: Embed
Units: unitless
Description: <p>Medium used to embed tissue sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/752581.rdf
Name: Number_of_Slides_Made
Units: count
Description: <p>Number of slides made from each block</p>
http://lod.bco-dmo.org/id/dataset-parameter/752582.rdf
Name: Number_Stained_with_H_E
Units: count
Description: <p>Number of slides stained with hematoxylin and eosin (H&E) stain</p>
http://lod.bco-dmo.org/id/dataset-parameter/752583.rdf
Name: Other_Stains
Units: unitless
Description: <p>Additional stains used on samples (Yes = Stain specified or N = No)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752584.rdf
Name: General_Condition_10x
Units: unitless
Description: <p>Observed tissue condition at 10x maginfication. Combines all parameters into a general score- 0=Excellent; 1= very good; 2= good; 3 =fair; 4 = poor; 5= very poor</p>
http://lod.bco-dmo.org/id/dataset-parameter/752585.rdf
Name: Zooxanthellae_10x
Units: unitless
Description: <p>Observed symbiont (zooxanthellae) condition at 10x magnification. 0=Excellent; 1= very good; 2= good; 3 =fair; 4 = poor; 5= very poor. Scores related to the number of zooxanthellae present and their overall apperance (atrophy or hypertrophy).</p>
http://lod.bco-dmo.org/id/dataset-parameter/752586.rdf
Name: Epidermal_Mucocytes_Condition
Units: unitless
Description: <p>Observed condition of the unicellular secretory gland cells that secrete mucus through an apical pore to aid in protection; sediment removal; and feeding. Epidermal mucocytes are those found in the epidermis; the outermost tissue layer in corals. Condition of mucocytes; 0 - not larger than ciliated columnar cells; uniform distribution; 1 - slightly hypertrophied; numerous; 2 - many hypertophied; abundant mucus release; 3 - uneven; hypertrophied and/or reduced in number; 4 - atrophy; necrosis; and epidermal foci lacking mucocytes; 5 - atrophy and most mucocytes lacking</p>
http://lod.bco-dmo.org/id/dataset-parameter/752587.rdf
Name: Mesenterial_Filament_Mucocytes
Units: unitless
Description: <p>Observed mucocyte condition in the cnidoglandular band area of mesenterial filaments. These are the internal longtiduinal partitions of tissue that provide structual support and increase surface area within the gastrovacular cavity. These regions aid in digestive processes and gonad development. 0=Excellent; 1= very good; 2= good; 3 =fair; 4 = poor; 5= very poor</p>
http://lod.bco-dmo.org/id/dataset-parameter/752588.rdf
Name: Degeneration_Cnidoglandular_Bands
Units: unitless
Description: <p>Observed condition of the free edge or middle ridge of the mesentary in the gastrovascular cavity. Scores related to reduction in cell height or number of cells; and atrophy of the epithelium in the cnidoglandular band. 0=Excellent; 1= very good; 2= good; 3 =fair; 4 = poor; 5= very poor</p>
http://lod.bco-dmo.org/id/dataset-parameter/752589.rdf
Name: Dissociation_of_Mesenterial_Filaments
Units: unitless
Description: <p>Observed condition of cells in the mesenterial filaments. Scores related to loss of cells and atrophy of cells. 0 - none to few; 1 - less than 1/2 area; not hypertrophied; 2 - about 1/2 area; some hypertrophied; 3 - about 1/2 area; all hypertrophied; 4 - about 3/4/ area; some vacuolated; 5 - much of area vacuolated; necrotic; loss of mucocytes</p>
http://lod.bco-dmo.org/id/dataset-parameter/752590.rdf
Name: Calicodermis_Condition
Units: unitless
Description: <p>Observed condition of the basal surface lining epithelium which contains calicoblasts- cells responsible for secreting a matrix to promote crystallization of calcium carbonate; thus assiting in the formation of coral skeleton. 0 = cells squamous but "thick;" cover mesoglea; 1 = cells slightly atrophied in some foci; 2 = 1/2 of cells atrophied; 3 = Most cells atrophied; beginning separation from mesoglea; 4 = Cells atrophied and loss or sloughing present; 5 = Multifocal necrosis or sloughing of atrophied cells or absent on mesoglea</p>
http://lod.bco-dmo.org/id/dataset-parameter/752591.rdf
Name: Calicodermis_Repair
Units: unitless
Description: <p>Is there evidence of repair of the calicodermis? Are there columnar calicoblasts with extensions of plasmallema present? (Present = 1; Not Present = 0)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752592.rdf
Name: Costal_Tissue_Loss
Units: unitless
Description: <p>Observed condition of tissue over the costal ridges (extensions of the septa of the corallite); 0 = none; tissue over costae intact; 1 = a few gaps noted; 2 = 1/4 of costae exposed; 3 = 1/2 of costae exposed; 4 = 3/4 of costae exposed; 5 = Most costae exposed; tissues attenuated; retracted</p>
http://lod.bco-dmo.org/id/dataset-parameter/752593.rdf
Name: Necrotic_Cell_Spherules
Units: unitless
Description: <p>Are there signs of necrotic (dying) cell or group of cells present? (Present = 1; Not Present = 0)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752594.rdf
Name: WBD_Bacterial_Aggregates
Units: count
Description: <p>Number of bacterial aggregates associated with white band disease observed.</p>
http://lod.bco-dmo.org/id/dataset-parameter/752595.rdf
Name: Epidermal_RLOs
Units: count
Description: <p>Number of Rickettsia-like organisms observed in the epidermal tissue layers.</p>
http://lod.bco-dmo.org/id/dataset-parameter/752596.rdf
Name: Filament_RLOs
Units: count
Description: <p>Number of Rickettsia-like organisms observed within the mesentary filaments.</p>
http://lod.bco-dmo.org/id/dataset-parameter/752597.rdf
Name: Gastrodermal_RLOs
Units: count
Description: <p>Number of Rickettsia-like organisms observed within the gastrodermis.</p>
http://lod.bco-dmo.org/id/dataset-parameter/752598.rdf
Name: Surface_Bacteria
Units: count
Description: <p>Number of bacteria present on the outer epidermal layer.</p>
http://lod.bco-dmo.org/id/dataset-parameter/752599.rdf
Name: Coccidia
Units: count
Description: <p>Number of single-celled intracellular parasites belonging to apicomplexans observed.</p>
http://lod.bco-dmo.org/id/dataset-parameter/752600.rdf
Name: Zooxanthellate_Ciliates
Units: unitless
Description: <p>Are single-celled protozoans with hair-like organelles called cilia observed with zooxanthellae? (Present = 1; Not Present = 0)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752601.rdf
Name: Non_zooxanthellate_Ciliates
Units: unitless
Description: <p>Are single-celled protozoans with hair-like organelles called cilia present in other locations of sample? (Present = 1; Not Present = 0)</p>
http://lod.bco-dmo.org/id/dataset-parameter/752602.rdf
Name: Oocytes_Stage_in_Development
Units: unitless
Description: <p>Egg cells found in the mesentaries: 0 - none present; 1 - single cells in mesoglea; 2 - early oocytes; 3 - mid-development; 4 - mature; 5 - spawned</p>
http://lod.bco-dmo.org/id/dataset-parameter/752603.rdf
Name: Spermaries_Stage_in_Development
Units: unitless
Description: <p>Location of developing spermatozoa in the mesentaries. 0 - none present; 1 - clusters of single cells in mesoglea; 2 - few; early spermaries; 3 - numerous spermaries; spermatocytes; 4 - mature; spermatozoa; 5 - spawned</p>
http://lod.bco-dmo.org/id/dataset-parameter/752604.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude coordinates with North positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/752605.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude coordinates with East positive</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
20118
https://darchive.mblwhoilibrary.org/bitstream/1912/26597/1/dataset-752550_coral-histology__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.752550.1
download
onLine
dataset
<p>Two 10 cm fragments (1 for control tank and 1 for treatment tank) from five colonies of each acroporid taxon were collected from Flat Cay the day prior to the experiment and left under ambient temperature conditions overnight. Tissue samples (2cm) were collected immediately prior to and immediately after the experiment and preserved in a Z-Fix Concentrate: seawater (1:4) solution. One fragment (putative genotype) from each taxon was placed in a control tank (n=5) and one was placed in a treatment tanks (n=5).&nbsp; Powerheads that were slightly tilted out of the water to provide aeration and water movement were placed in all tanks. The ambient tanks were placed in a large tank with constant flowing seawater that served as a water bath. The five treatment tanks, each with an individual heater, were place in a neighboring large tank with no running seawater. All tanks were under a covered porch, but exposed to ambient light conditions.&nbsp;</p>
<p>A Hobo temperature logger was placed in each tank and a water temperature measurement was collected every 30 seconds. The control tanks ranged from 27.5 to 29.4C with an average of 28.2C and the heated tanks ranged from 29.1 to 32.7C with an average of 31.3C over the 48 hour experimental period.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>The semi-quantitative rubric was designed by Dr. Esther Peters for the histological analysis.&nbsp;&nbsp;</p>
<p>Data legend:<br />
ND = No Data<br />
NS = not enough tissue to determine, not seen</p>
<p>Lesion Description:&nbsp;<br />
H = healthy<br />
WBD = white band disease<br />
RTL = rapid tissue loss<br />
BL = bleaching</p>
<p>Bleaching:&nbsp;<br />
0 = none<br />
1 = paling<br />
2 = bleaching</p>
<p>Bleaching Distribution:<br />
1 = focal<br />
2 = multifocal distinct<br />
3 = multifocal indistinct<br />
4 = diffuse</p>
<p>Condition Scores:&nbsp;<br />
0 = excellent<br />
1 = very good<br />
2 = good<br />
3 = fair<br />
4 = poor<br />
5 = very poor</p>
<p>Intensity/Severity Scores:<br />
0 = no change<br />
1 = minimal<br />
2 = mild<br />
3 = moderate<br />
4 = marked<br />
5 = severe</p>
<p>Other:<br />
P = present<br />
A = absent<br />
SI = staining issue<br />
NS = not seen<br />
N/A = not available<br />
N = no</p>
<p>Epidermal mucocytes:<br />
0 = not larger than ciliated columnar cells, uniform distribution<br />
1 = slightly hypertrophied, numerous<br />
2 =&nbsp; many hypertophied, abundant mucus release<br />
3 =&nbsp; uneven, hypertrophied vs reduced&nbsp;<br />
4 =&nbsp; atrophy, necrosis, and epidermal foci lacking mucocytes<br />
5 =&nbsp; atrophy and most mucocytes lacking</p>
<p>Mesenterial Filament (CGB Epithelium) Mucocytes:<br />
0 = none to few<br />
1 = less than 1/2 area, not hypertrophied<br />
2 = about 1/2 area, some hypertrophied<br />
3 = about 1/2 area, all hypertrophied&nbsp;<br />
4 = about 3/4/ area, some vacuolated<br />
5 = much of area vacuolated, necrotic, loss of mucocytes</p>
<p>Calicodermis Condition:<br />
0 = cells squamous but "thick," cover mesoglea<br />
1 = cells slightly atrophied in some foci<br />
2 = 1/2 of cells atrophied<br />
3 = Most cells atrophied, beginning separation from mesoglea<br />
4 = Cells atrophied and loss or sloughing present</p>
<p>Costal Tissue Loss:<br />
0 = none, tissue over costae intact<br />
1 = a few gaps noted<br />
2 = 1/4 of costae exposed<br />
3 = 1/2 of costae exposed<br />
4 = 3/4 of costae exposed<br />
5 = Most costae exposed, tissues attenuated, retracted?</p>
<p>Oocytes:<br />
0 - none present<br />
1 - single cells in mesoglea<br />
2 - early oocytes<br />
3 - mid-development<br />
4 - mature<br />
5 - spawned</p>
<p>Spermaries:<br />
0 - none present<br />
1 - clusters of single cells in mesoglea<br />
2 - few, early spermaries<br />
3 - numerous spermaries, spermatocytes<br />
4 - mature, spermatozoa<br />
5 - spawne</p>
<p>BCO-DMO Processing Notes:</p>
<ul>
<li>added conventional header with dataset name, PI name, version date</li>
<li>modified parameter names to conform with BCO-DMO naming conventions</li>
<li>added lat, lon columns</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Hobo Pro v2 data logger
Hobo Pro v2 data logger
PI Supplied Instrument Name: Hobo Pro v2 data logger PI Supplied Instrument Description:A Hobo temperature logger was placed in each tank and a water temperature measurement was collected every 30 seconds. Instrument Name: Temperature Logger Instrument Short Name: Instrument Description: Records temperature data over a period of time.