http://lod.bco-dmo.org/id/dataset/752613 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2019-01-08 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Field temperature data from estuaries along the Atlantic coast of North America containing Nematostella vectensis from deployments between March of 2016 and January of 2017 2019-01-11 publication 2019-01-11 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2021-02-10 publication https://doi.org/10.26008/1912/bco-dmo.752613.2 Adam Reitzel University of North Carolina at Charlotte principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Reitzel, A. (2021) Field temperature data from estuaries along the Atlantic coast of North America containing Nematostella vectensis from deployments between March of 2016 and January of 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2019-01-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.752613.2 [access date] Methods and Sampling: HOBO temperature loggers where deployed in tidally restricted pools in estuaries along the Atlantic coast of North America. Loggers were fastened to pipes that were dug into these pools. Problem report: In this data set, the temperature logger for Maine failed to log temperature data after September 6th. All other locations have complete data sets from March 11 until December 31, 2016. Locations: St. Augustine, Florida: 29.72642,-81.25772 Georgetown, South Carolina: 33.33183,-79.19994 Ft. Fisher, North Carolina: 33.959,-77.93283 Sippewissett, Massachusetts: 41.58939,-70.63797 Pine Point, Maine: 43.54261,-70.34497 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536530 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536530 completed Adam Reitzel University of North Carolina at Charlotte 704-687-5018 9201 University City Blvd. Woodward Hall, 381A Charlotte NC 28223 USA areitze2@uncc.edu pointOfContact asNeeded Dataset Version: 2 Unknown Data_Point Date_Time ISO_DateTime_UTC Site Temp lat lon HOBO pendant temperature loggers (Onset, UA-002-64) theme None, User defined sample identification Date and time ISO_DateTime_UTC site water temperature at measurement depth latitude longitude featureType BCO-DMO Standard Parameters Temperature Logger instrument BCO-DMO Standard Instruments Reitzel_Estuarine_Temp service Deployment Activity Atlantic coast of North America place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Toxin expression and function by an estuarine model species in a dynamic seasonal community https://osprey.bco-dmo.org/project/560140 Toxin expression and function by an estuarine model species in a dynamic seasonal community <p><em>Description from NSF award abstract:</em><br /> Venomous animals such as cnidarians, scorpions, and snakes utilize their toxins for a diversity of functions including defense from predators and for capturing prey. Thus, venom plays a pivotal and central role in the ecology of the animal producing it. There are several major hypotheses regarding the ecological factors that shape the diversity and expression of these molecules. A central hypothesis is that these toxins evolve rapidly due to their involvement in an evolutionary "arms race" between the organism and its potential predators and prey. Moreover, toxins are energetically expensive to produce so it is also hypothesized that their production is tightly regulated ("venom optimization hypothesis"), where individuals can module the amount and composition of their venom dependent on changes in diet and shifts in environmental factors such as temperature. Currently, there is a paucity of data to discern between these hypotheses. This research will provide an integrative approach to identify mechanisms that determine shifts in the quantity and composition of the venom essential for the feeding ecology of a model cnidarian specializing in estuarine habitats that are vulnerable to the ongoing climate change. This project will provide a synergistic international collaboration with Dr. Yehu Moran (The Hebrew University of Jerusalem, Israel) to connect environmental variation with the expression and potency of venom proteins. This research will provide mentoring and training for a postdoctoral fellow, graduate and undergraduate students at the University of North Carolina at Charlotte, and undergraduate students at Mitchell Community College in Statesville, NC. Additional research and educational opportunities will be provided for underrepresented students using PRODUCE, funded by the NSF through the NC Louis Stokes Alliance for Minority Participation to recruit, retain, and graduate students in STEM education. The PI will continue to involve high-school students in research through individual training, UNC Charlotte's outreach programs and the Burroughs Wellcome Science Enrichment program with the Olympic High School Biotechnology School.</p> <p>Knowledge of the environmental, genetic, and molecular factors involved in modulating expression of toxin proteins is essential to understand and predict how venomous species will respond to changes in the composition of biological communities. The research team will rigorously test hypotheses in the expression of toxin peptides by studying the starlet sea anemone, Nematostella vectensis, a predator of ecological importance that lives in estuaries along the eastern coast of North America. N. vectensis has emerged as a model cnidarian for ecological genomics due to the availability of a genome, its central position in estuarine food webs, and the ease of field collection and laboratory manipulation; a combination rarely available for venomous animals. Expression of venom genes in field collected anemones will be correlated with ingested prey identified using microscopy and DNA barcodes. This analysis will be compared in three different seasons to identify impacts of seasonal changes in abiotic variables as well as prey availability. Differences in expression of these toxin genes in the field could be related to tuning of the toxin composition to the prey available, presence of predators, and/or the physiological condition of the individual determined by degree of abiotic stress. The research team will differentiate between these potential causal factors through controlled laboratory experiments where anemones are exposed to two common stressors (temperature, UV), individual prey items, and a predator. In order to better understand the selectivity of these toxins to particular prey, behavioral and in vitro assays test toxicity of each genetic variant will be compared when exposed to distinct animal prey groups to determine the relative potency. Finally, sequence analyses will be used to investigate the molecular targets of the toxins in the prey animals to determine if these targets are potentially adapted to toxin alleles restricted to different geographic locations. These data will help determine a potential causative link between toxin evolution of a venomous animal and its prey and whether toxins and their targets are involved in an "arms race". This project is expected to have a significant impact on the understanding of the evolution of toxins in the context of ecological interactions in natural environments and contribute to our knowledge about toxin pharmacology for future design of novel insecticides.</p> Cnidarian Toxin Dynamics largerWorkCitation project eng; USA inlandWaters oceans Atlantic coast of North America -81.25772 -70.34497 29.72642 43.54261 2016-03-11 2017-01-01 Georgetown, South Carolina; and Woods Hole, Massachusetts 0 BCO-DMO catalogue of parameters from Field temperature data from estuaries along the Atlantic coast of North America containing Nematostella vectensis from deployments between March of 2016 and January of 2017 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/752712.rdf Name: Data_Point Units: unitless Description: <p>Numeric identifier for data point</p> http://lod.bco-dmo.org/id/dataset-parameter/752713.rdf Name: Date_Time Units: unitless Description: <p>Date and Time (local,UTC-5) in format &#039;mm/dd/yyyy HH:MM&#039;</p> http://lod.bco-dmo.org/id/dataset-parameter/752714.rdf Name: ISO_DateTime_UTC Units: unitless Description: <p>Timestamp with time zone (UTC) in standard ISO 8601:2004(E) format 'yyyy-mm-ddTHH:MMZ'</p> http://lod.bco-dmo.org/id/dataset-parameter/752715.rdf Name: Site Units: unitless Description: <p>Site of temperature deployment</p> http://lod.bco-dmo.org/id/dataset-parameter/752716.rdf Name: Temp Units: degrees Celsius (C) Description: <p>Water temperature from HOBO logger</p> http://lod.bco-dmo.org/id/dataset-parameter/752717.rdf Name: lat Units: decimal degrees (DD) Description: <p>Latitude</p> http://lod.bco-dmo.org/id/dataset-parameter/752718.rdf Name: lon Units: decimal degrees (DD) Description: <p>Longitude</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 8056031 https://darchive.mblwhoilibrary.org/bitstream/1912/26667/1/dataset-752613_field-temperature-data__v2.tsv download https://doi.org/10.26008/1912/bco-dmo.752613.2 download onLine dataset HOBO temperature loggers where deployed in tidally restricted pools in estuaries along the Atlantic coast of North America. Loggers were fastened to pipes that were dug into these pools. Problem report: In this data set, the temperature logger for Maine failed to log temperature data after September 6th. All other locations have complete data sets from March 11 until December 31, 2016. Locations: St. Augustine, Florida: 29.72642,-81.25772 Georgetown, South Carolina: 33.33183,-79.19994 Ft. Fisher, North Carolina: 33.959,-77.93283 Sippewissett, Massachusetts: 41.58939,-70.63797 Pine Point, Maine: 43.54261,-70.34497 Specified by the Principal Investigator(s) No processing performed, these are the raw data from the temperature logger. ------------------------------------------------------------------------------------------------------------------------------------ BCO-DMO Data Manager Processing Notes: Data version 1: 2019-01-08 * added a conventional header with dataset name, PI name, version date * modified parameter names to conform with BCO-DMO naming conventions * blank values in this dataset are displayed as &quot;nd&quot; for &quot;no data.&quot; nd is the default missing data identifier in the BCO-DMO system. * ISO_DateTime_UTC added from local date and time column. -------------------------------------- Data version 2: 2019-01-11 * Five Temperature columns were unpivoted into one temperature columns with new column &quot;Site&quot; which contains the location specified in the original column name. Temp_Florida,Temp_South_Carolina,Temp_North_Carolina,Temp_Massachusetts,Temp_Maine -&gt; 37.384,24.738,29.652,15.76,2.624 to Temp,Site 37.384,Florida 24.738,South_Carolina ... * Lat and lon columns added to the dataset ------------------------------------------------------------------------------------------------------------------------------------ Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact HOBO pendant temperature loggers (Onset, UA-002-64) HOBO pendant temperature loggers (Onset, UA-002-64) PI Supplied Instrument Name: HOBO pendant temperature loggers (Onset, UA-002-64) Instrument Name: Temperature Logger Instrument Short Name: Instrument Description: Records temperature data over a period of time. Deployment: Reitzel_Estuarine_Temp Reitzel_Estuarine_Temp shoreside Eastern United States shoreside Reitzel_Estuarine_Temp Adam Reitzel University of North Carolina at Charlotte shoreside Eastern United States shoreside