http://lod.bco-dmo.org/id/dataset/752974
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-01-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in different pCO2 conditions, July 2017
2019-01-14
publication
2019-01-14
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-09-25
publication
https://doi.org/10.1575/1912/bco-dmo.752974.1
Shawn M. Arellano
Western Washington University
principalInvestigator
M. Brady Olson
Western Washington University
principalInvestigator
Sylvia Yang
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Arellano, S., Olson, B., Yang, S. (2019) Body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in different pCO2 conditions, July 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-01-14 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.752974.1 [access date]
Dendraster Characters OA Expt2017 Dataset Description: <p>This dataset includes body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in different pCO2 conditions as part of a laboratory experiment to investigate the behavioral effects of ocean acidification on this species in July 2017.</p> Methods and Sampling: <p>We collected adult sand dollars (D. excentricus) from Semiahmoo Bay, WA, in July 2017 and maintained them in 14°C continuous flowing seawater at the Shannon Point Marine Center. We induced twelve individuals to spawn by injecting 1-mL of 0.5-M KCl into the coelom following methods outlined by Strathmann (1987).&nbsp; We then collected and mixed concentrated gametes of four males and four females for fertilization. We added five drops of sperm to 500-mL of filtered seawater and 5-mL of eggs. We placed the fertilized eggs in 12°C incubator and bubbled them with ambient pCO2 condition for 12-hrs before dividing the embryos into pCO2 treatment conditions before gastrulation.</p>
<p>We reared D. excentricus larvae (2 individuals mL-1) at 12°C in eight 3-L jars that were individually bubbled with CO2 to achieve four replicates of ambient (400ppm) and acidic (1500ppm) pCO2 conditions. Daily we gave each larval jar a water change from pre-equilibrated ambient and acidic water and fed the larvae D. tertiolecta (6,000 cells ml-1).</p>
<p>We measured morphological characteristics of larvae from each rearing jar when larvae were in the 4- and 6-arm stages following diagrams in Chan et al. (2011) and Reitzel et al. (2004). Larvae were relaxed in 7% magnesium chloride, fixed in 3% formalin, imaged using a stereomicroscope equipped with a camera (Leica MC170 HD and Leica Application Suite, Leica, Wetzlar, Germany), and measured with ImageJ software. 4-arm larvae were fixed on 7/24/17 and 6-arm larvae were fixed on 7/28/18. The following morphological characteristics were measured: length of postoral arms (PO), anterolateral arms (AL), length of body midline, body width, and longitudinal length of stomach.</p>
<p>This dataset includes unprocessed data.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538626 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538626
completed
Shawn M. Arellano
Western Washington University
360-650-3634
Western Washington University 1900 Shannon Point Road
Anacortes
WA
98221
USA
arellas@wwu.edu
pointOfContact
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Sylvia Yang
Western Washington University
360-598-4460
SEA Discovery Center P.O. Box 2318
Poulsbo
WA
98370
Sylvia.yang@wwu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
rearing_condition
jar_replicate
PO_1
PO_2
AL_1
AL_2
Body_ML
BW
PD_1
PD_2
Stomach
theme
None, User defined
date_local
treatment
replicate
length
featureType
BCO-DMO Standard Parameters
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
https://osprey.bco-dmo.org/project/684167
RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
<p>In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences</p>
<p>As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.</p>
Climate stressors on larvae
largerWorkCitation
project
eng; USA
biota
oceans
2017-07-21
2017-07-27
Coastal Pacific, USA
0
BCO-DMO catalogue of parameters from Body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in different pCO2 conditions, July 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/752986.rdf
Name: Date
Units: unitless
Description: <p>Date larvae were fixed formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/752988.rdf
Name: rearing_condition
Units: unitless
Description: <p>The pH condition of the water larvae were reared in; "acidic" water was bubbled to be 1500ppm and "ambient" water was bubbled to be 400ppm</p>
http://lod.bco-dmo.org/id/dataset-parameter/752989.rdf
Name: jar_replicate
Units: unitless
Description: <p>Jar replicate larvae were sampled from. There were four replicate jars per rearing condition.</p>
http://lod.bco-dmo.org/id/dataset-parameter/752990.rdf
Name: PO_1
Units: micrometers (um)
Description: <p>Postoral arm length; one of two</p>
http://lod.bco-dmo.org/id/dataset-parameter/752991.rdf
Name: PO_2
Units: micrometers (um)
Description: <p>Postoral arm length; one of two</p>
http://lod.bco-dmo.org/id/dataset-parameter/752992.rdf
Name: AL_1
Units: micrometers (um)
Description: <p>Anterolateral arm length; one of two</p>
http://lod.bco-dmo.org/id/dataset-parameter/752993.rdf
Name: AL_2
Units: micrometers (um)
Description: <p>Anterolateral arm length; one of two</p>
http://lod.bco-dmo.org/id/dataset-parameter/752994.rdf
Name: Body_ML
Units: micrometers (um)
Description: <p>Length of body midline</p>
http://lod.bco-dmo.org/id/dataset-parameter/752995.rdf
Name: BW
Units: micrometers (um)
Description: <p>Length of body width</p>
http://lod.bco-dmo.org/id/dataset-parameter/752996.rdf
Name: PD_1
Units: micrometers (um)
Description: <p>Posterodorsal arm length; one of two</p>
http://lod.bco-dmo.org/id/dataset-parameter/752997.rdf
Name: PD_2
Units: micrometers (um)
Description: <p>Posterodorsal arm length; one of two</p>
http://lod.bco-dmo.org/id/dataset-parameter/752998.rdf
Name: Stomach
Units: micrometers (um)
Description: <p>Longitudinal length of stomach</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11043
https://darchive.mblwhoilibrary.org/bitstream/1912/24621/1/dataset-752974_dendrastercharactersoaexpt2017__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.752974.1
download
onLine
dataset
<p>We collected adult sand dollars (D. excentricus) from Semiahmoo Bay, WA, in July 2017 and maintained them in 14°C continuous flowing seawater at the Shannon Point Marine Center. We induced twelve individuals to spawn by injecting 1-mL of 0.5-M KCl into the coelom following methods outlined by Strathmann (1987).&nbsp; We then collected and mixed concentrated gametes of four males and four females for fertilization. We added five drops of sperm to 500-mL of filtered seawater and 5-mL of eggs. We placed the fertilized eggs in 12°C incubator and bubbled them with ambient pCO2 condition for 12-hrs before dividing the embryos into pCO2 treatment conditions before gastrulation.</p>
<p>We reared D. excentricus larvae (2 individuals mL-1) at 12°C in eight 3-L jars that were individually bubbled with CO2 to achieve four replicates of ambient (400ppm) and acidic (1500ppm) pCO2 conditions. Daily we gave each larval jar a water change from pre-equilibrated ambient and acidic water and fed the larvae D. tertiolecta (6,000 cells ml-1).</p>
<p>We measured morphological characteristics of larvae from each rearing jar when larvae were in the 4- and 6-arm stages following diagrams in Chan et al. (2011) and Reitzel et al. (2004). Larvae were relaxed in 7% magnesium chloride, fixed in 3% formalin, imaged using a stereomicroscope equipped with a camera (Leica MC170 HD and Leica Application Suite, Leica, Wetzlar, Germany), and measured with ImageJ software. 4-arm larvae were fixed on 7/24/17 and 6-arm larvae were fixed on 7/28/18. The following morphological characteristics were measured: length of postoral arms (PO), anterolateral arms (AL), length of body midline, body width, and longitudinal length of stomach.</p>
<p>This dataset includes unprocessed data.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- reformatted date from m/d/yy to yyyy-mm-dd<br />
- changed year from 2018 to 2017</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact