http://lod.bco-dmo.org/id/dataset/753036
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-01-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Lipid analysis data from experiment on grazing and physiological effects of ocean acidification on sand dollar larvae (Dendraster excentricus), July 2017
2019-01-14
publication
2019-01-14
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-09-25
publication
https://doi.org/10.1575/1912/bco-dmo.753036.1
Shawn M. Arellano
Western Washington University
principalInvestigator
M. Brady Olson
Western Washington University
principalInvestigator
Sylvia Yang
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Arellano, S., Olson, B., Yang, S. (2019) Lipid analysis data from experiment on grazing and physiological effects of ocean acidification on sand dollar larvae (Dendraster excentricus), July 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-01-14 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.753036.1 [access date]
Lipid_Dendraster_OA_Expt2017 Dataset Description: <p>Lipid analysis data collected from a laboratory experiment to investigate the grazing and physiological effects of ocean acidification on sand dollar larvae (<em>Dendraster excentricus</em>), July 2017.</p> Methods and Sampling: <p><em>Spawning and fertilization</em></p>
<p>We collected adult sand dollars (<em>D. excentricus</em>) from Semiahmoo Bay, WA, on July 7, 2017, and maintained them in 14°C continuous flowing seawater at the Shannon Point Marine Center. On July 12, 2017, we induced twelve individuals to spawn by injecting 1-mL of 0.5-M KCl into the coelom following methods outlined by Strathmann (1987).&nbsp; We then collected and mixed concentrated gametes of four males and four females for fertilization. We added five drops of sperm to 500-mL of filtered seawater and 5-mL of eggs. We placed the fertilized eggs in 12°C incubator and bubbled them with ambient pCO2 condition for 12-hrs before dividing the embryos into pCO2 treatment conditions before gastrulation. We then counted and transferred the larvae into jars with 1.5 L of nanopore filtered seawater at densities of 1-2 individuals mL<sup>-1</sup>.</p>
<p><em>Grazing experiment</em></p>
<p>To assess the interactive effects of high temperature and pCO<sub>2</sub> on <em>Dunaliella excentricus</em> feeding behavior, our experimental design had six treatments with four experimental jars (replicates) in each. The treatments combined three levels of CO<sub>2</sub>: 400 ppmv (ambient atmospheric level), 800 ppmv (moderate atmoshpheric level) and 1,500 ppmv (high atmospheric level), and two temperatures: 12°C (ambient temperature) and 17°C (high temperature). We fed <em>D. tertiolecta</em> at approximately 6,000 cells ml<sup>-1</sup> to six-arm stage larvae to evaluate feeding rates at each treatment condition.</p>
<p>For each replicate, a corresponding 150-mL control bottle containing only <em>D. tertiolecta</em> was also prepared. Feeding rate was estimated as ingestion rate by measuring the algal concentration (cells ml<sup>-1</sup>) at the beginning (T<sub>0</sub>) and after 24 hours (T<sub>f</sub>) in control bottles and experimental jars using a Sedgewick Rafter Chamber (Stumpp et al., 2011). Ingestion rate (cells ind<sup>-1</sup> hr<sup>-1</sup>) was calculated as I = (Clearance rate) x (time-average algae concentration).&nbsp;</p>
<p><em>Lipid storage analysis</em></p>
<p>At the end of the long-term experiment, larval lipid index was assessed using a procedure adapted from Talmage et. al (2010).&nbsp; First, we randomly selected <em>D. excentricus</em> larvae from each treatment and stained them with Nile Red dissolved in acetone. Nile Red stains intracellular lipid droplets bright yellow. Larvae were exposed to the stain for ∼1.5 h, after which they were photographed under an epi-fluorescent microscope (Leica 80i) within 4 hours of being stained&nbsp; (Ko et al., 2014). The lipid areas of approximately 5−15 larvae per sample were measured using the ImageJ software. The lipid index was calculated by dividing the area of the larva stomach containing the fluorescing lipids by the total stomach area (Talmage &amp; Gobler, 2010).&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538626 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538626
completed
Shawn M. Arellano
Western Washington University
360-650-3634
Western Washington University 1900 Shannon Point Road
Anacortes
WA
98221
USA
arellas@wwu.edu
pointOfContact
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Sylvia Yang
Western Washington University
360-598-4460
SEA Discovery Center P.O. Box 2318
Poulsbo
WA
98370
Sylvia.yang@wwu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
pH_treatment
temp_treatment
jar_replicate
lipid_presence
lipid_area
stomach_area
lipid_index
epi-fluorescent microscope (Leica 80i)
theme
None, User defined
treatment
replicate
flag
surface_area
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Fluorescence Microscope
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
https://osprey.bco-dmo.org/project/684167
RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
<p>In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences</p>
<p>As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.</p>
Climate stressors on larvae
largerWorkCitation
project
eng; USA
biota
oceans
2017-07-07
2017-07-31
Coastal Pacific, USA
0
BCO-DMO catalogue of parameters from Lipid analysis data from experiment on grazing and physiological effects of ocean acidification on sand dollar larvae (Dendraster excentricus), July 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/753045.rdf
Name: pH_treatment
Units: unitless
Description: <p>The pH condition of the water larvae were reared within: L = treatment of 400ppm; M = treatment of 800pm; H = treatment of 1500ppm</p>
http://lod.bco-dmo.org/id/dataset-parameter/753046.rdf
Name: temp_treatment
Units: degrees Celsius
Description: <p>The temperature condition of the water larvae were reared within</p>
http://lod.bco-dmo.org/id/dataset-parameter/753047.rdf
Name: jar_replicate
Units: unitless
Description: <p>Replicate of the pH and temperature treatment combination. Four jars were maintained at each treatment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/753048.rdf
Name: lipid_presence
Units: unitless
Description: <p>Is lipid present within measured larva? Yes or No</p>
http://lod.bco-dmo.org/id/dataset-parameter/753049.rdf
Name: lipid_area
Units: pixels per micron
Description: <p>Area of larva's lipid content</p>
http://lod.bco-dmo.org/id/dataset-parameter/753050.rdf
Name: stomach_area
Units: pixels per micron
Description: <p>Area of larva's stomach</p>
http://lod.bco-dmo.org/id/dataset-parameter/753051.rdf
Name: lipid_index
Units: unitless
Description: <p>Area of larva's lipid content divided by area of larva's stomach</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10053
https://darchive.mblwhoilibrary.org/bitstream/1912/24618/1/dataset-753036_lipiddendrasteroaexpt2017__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.753036.1
download
onLine
dataset
<p><em>Spawning and fertilization</em></p>
<p>We collected adult sand dollars (<em>D. excentricus</em>) from Semiahmoo Bay, WA, on July 7, 2017, and maintained them in 14°C continuous flowing seawater at the Shannon Point Marine Center. On July 12, 2017, we induced twelve individuals to spawn by injecting 1-mL of 0.5-M KCl into the coelom following methods outlined by Strathmann (1987).&nbsp; We then collected and mixed concentrated gametes of four males and four females for fertilization. We added five drops of sperm to 500-mL of filtered seawater and 5-mL of eggs. We placed the fertilized eggs in 12°C incubator and bubbled them with ambient pCO2 condition for 12-hrs before dividing the embryos into pCO2 treatment conditions before gastrulation. We then counted and transferred the larvae into jars with 1.5 L of nanopore filtered seawater at densities of 1-2 individuals mL<sup>-1</sup>.</p>
<p><em>Grazing experiment</em></p>
<p>To assess the interactive effects of high temperature and pCO<sub>2</sub> on <em>Dunaliella excentricus</em> feeding behavior, our experimental design had six treatments with four experimental jars (replicates) in each. The treatments combined three levels of CO<sub>2</sub>: 400 ppmv (ambient atmospheric level), 800 ppmv (moderate atmoshpheric level) and 1,500 ppmv (high atmospheric level), and two temperatures: 12°C (ambient temperature) and 17°C (high temperature). We fed <em>D. tertiolecta</em> at approximately 6,000 cells ml<sup>-1</sup> to six-arm stage larvae to evaluate feeding rates at each treatment condition.</p>
<p>For each replicate, a corresponding 150-mL control bottle containing only <em>D. tertiolecta</em> was also prepared. Feeding rate was estimated as ingestion rate by measuring the algal concentration (cells ml<sup>-1</sup>) at the beginning (T<sub>0</sub>) and after 24 hours (T<sub>f</sub>) in control bottles and experimental jars using a Sedgewick Rafter Chamber (Stumpp et al., 2011). Ingestion rate (cells ind<sup>-1</sup> hr<sup>-1</sup>) was calculated as I = (Clearance rate) x (time-average algae concentration).&nbsp;</p>
<p><em>Lipid storage analysis</em></p>
<p>At the end of the long-term experiment, larval lipid index was assessed using a procedure adapted from Talmage et. al (2010).&nbsp; First, we randomly selected <em>D. excentricus</em> larvae from each treatment and stained them with Nile Red dissolved in acetone. Nile Red stains intracellular lipid droplets bright yellow. Larvae were exposed to the stain for ∼1.5 h, after which they were photographed under an epi-fluorescent microscope (Leica 80i) within 4 hours of being stained&nbsp; (Ko et al., 2014). The lipid areas of approximately 5−15 larvae per sample were measured using the ImageJ software. The lipid index was calculated by dividing the area of the larva stomach containing the fluorescing lipids by the total stomach area (Talmage &amp; Gobler, 2010).&nbsp;</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- reduced precision of lipid_index from 9 to 3 decimals</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
epi-fluorescent microscope (Leica 80i)
epi-fluorescent microscope (Leica 80i)
PI Supplied Instrument Name: epi-fluorescent microscope (Leica 80i) PI Supplied Instrument Description:Used to photograph larval intracellular lipid droplets. Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/