http://lod.bco-dmo.org/id/dataset/753058
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-01-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Oyster larvae vertical distribution data collected from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017
2019-01-14
publication
2019-01-14
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-09-25
publication
https://doi.org/10.1575/1912/bco-dmo.753058.1
Shawn M. Arellano
Western Washington University
principalInvestigator
M. Brady Olson
Western Washington University
principalInvestigator
Sylvia Yang
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Arellano, S., Olson, B., Yang, S. (2019) Oyster larvae vertical distribution data collected from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-01-14 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.753058.1 [access date]
Ostrea_Behavior_OA_Expt2017 Dataset Description: <p>Oyster larvae vertical distribution data collected from a laboratory water column experiments to investigate the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017.</p> Methods and Sampling: <p>Collection &amp; Larval Rearing</p>
<p>&nbsp;We collected adult Olympia oysters (<em>Ostrea </em><em>lurida</em>) from Fidalgo Bay in June 2017 and maintained them in a sea table with continuous flowing seawater heated to 19-20°C at the Shannon Point Marine Center. We fed adult oysters were fed concentrated algae once a day (Shellfish Diet, Reed Mariculture) and utilized banjo-style filters (60-m) attached to the outflow pipes of the sea table to catch released <em>O. </em><em>lurida</em> larvae. We then collected and reared larvae at 12°C in 3-L jars (2 individuals mL-1). Each jar of larvae received a 50% water change with 0.35-m filtered sea water and were fed <em>Isochrysis </em><em>galbana</em> algae (50,000 cells mL-1) daily.</p>
<p>&nbsp;Experimental Design</p>
<p>&nbsp;To measure the effect of pH conditions on the vertical distribution of larvae we established three experimental pycnocline treatments within clear plexiglass water columns (2.5cm x 2.5cm x 30cm): (1) ambient water (400ppm) in the top layer and acidic water in the bottom layer (1500ppm), (2) ambient water (400ppm) in both top and bottom layers, and (3) acidic water (1500ppm) in the top layer and ambient water (400ppm) in the bottom layer. Each water layer was 60-mL of water and filled the column 10-cm high, so when each experimental treatment was established it filled the column to 20-cm. We established the experimental treatments by increasing the density of seawater in the bottom layer by 0.003-0.005 g ml-1 using PercollTM GE Healthcare (Podolsky &amp; Emlet 1993). Experimental treatment water was kept at 12°C and pre-equilibrated to the desired pCO2 level and density. We also included blue food coloring (1 drop per 100-mL) to the dense bottom layer to more easily visualize the density layers while establishing experimental treatments. We set-up four replicate columns for each experimental treatment making twelve columns total per experiment.</p>
<p>&nbsp;On the day of each experiment, we incubated the experimental treatment columns in clear plexiglass water baths connected to a Fisher Scientific Isotemp recirculating water bath to maintain treatment temperature at 12°C throughout the experiment. We carefully injected 150 larvae by syringe into the bottom 2-cm of each column with no more than 2-mL of their culture water. Olympia oyster larvae are highly phototactic (personal observations), so larvae were kept in the dark and we video recorded their vertical positions under infrared light two times: the first time at 10 minutes of acclimation in the columns and the second time at 30 minutes of acclimation in the columns. To record, we used an infrared uEye camera equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand. We later counted by eye the number of larvae per centimeter area of each column from the videos.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538626 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538626
completed
Shawn M. Arellano
Western Washington University
360-650-3634
Western Washington University 1900 Shannon Point Road
Anacortes
WA
98221
USA
arellas@wwu.edu
pointOfContact
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Sylvia Yang
Western Washington University
360-598-4460
SEA Discovery Center P.O. Box 2318
Poulsbo
WA
98370
Sylvia.yang@wwu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
trial
date
column_name
count_id
height_cm
middepth_cm
larvae_count
proportion_larvae
uEye video camera
Fisher Scientific Isotemp Circulating Water Bath
theme
None, User defined
experiment id
date_local
sample identification
replicate
height
depth
count
relative abundance
featureType
BCO-DMO Standard Parameters
Camera
In-situ incubator
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
https://osprey.bco-dmo.org/project/684167
RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
<p>In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences</p>
<p>As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.</p>
Climate stressors on larvae
largerWorkCitation
project
eng; USA
biota
oceans
2017-07-21
2017-08-03
Coastal Pacific, USA
0
BCO-DMO catalogue of parameters from Oyster larvae vertical distribution data collected from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/753072.rdf
Name: trial
Units: unitless
Description: <p>Trial number</p>
http://lod.bco-dmo.org/id/dataset-parameter/753073.rdf
Name: date
Units: unitless
Description: <p>Date of trial formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/753074.rdf
Name: column_name
Units: unitless
Description: <p>Identifies the experimental water column treatment and replicate: AN-# = Acidic water at the top and Neutral water at the bottom ; NN-# = Neutral water at the top and Neutral water at the bottom; NA-# = Neutral water at the top and Acid water at the bottom. Acidic water was bubbled to be 400 pCO2 and neutral (ambient) water was bubbled to be 1500 pCO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/753075.rdf
Name: count_id
Units: unitless
Description: <p>Identifies the count number (1 or 2) per experimental date. The vertical positions of larvae in the columns were counted twice for each experiment; the first count at 10 minutes post-larval introduction into the column and the second count at 30 minutes post-larval introduction into the column.</p>
http://lod.bco-dmo.org/id/dataset-parameter/753076.rdf
Name: height_cm
Units: centimeters (cm)
Description: <p>The height above the bottom of the water column where larvae were counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/753077.rdf
Name: middepth_cm
Units: centimeters (cm)
Description: <p>The middepth of the section of the water column in which larvae were counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/753078.rdf
Name: larvae_count
Units: larvae
Description: <p>The number of Olympia oyster larvae occupying that area of the water column during the count</p>
http://lod.bco-dmo.org/id/dataset-parameter/753079.rdf
Name: proportion_larvae
Units: unitless
Description: <p>Proportion of total Olympia oyster larvae occupying that area of the water column during the count</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
24769
https://darchive.mblwhoilibrary.org/bitstream/1912/24617/1/dataset-753058_ostreabehavioroaexpt2017__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.753058.1
download
onLine
dataset
<p>Collection &amp; Larval Rearing</p>
<p>&nbsp;We collected adult Olympia oysters (<em>Ostrea </em><em>lurida</em>) from Fidalgo Bay in June 2017 and maintained them in a sea table with continuous flowing seawater heated to 19-20°C at the Shannon Point Marine Center. We fed adult oysters were fed concentrated algae once a day (Shellfish Diet, Reed Mariculture) and utilized banjo-style filters (60-m) attached to the outflow pipes of the sea table to catch released <em>O. </em><em>lurida</em> larvae. We then collected and reared larvae at 12°C in 3-L jars (2 individuals mL-1). Each jar of larvae received a 50% water change with 0.35-m filtered sea water and were fed <em>Isochrysis </em><em>galbana</em> algae (50,000 cells mL-1) daily.</p>
<p>&nbsp;Experimental Design</p>
<p>&nbsp;To measure the effect of pH conditions on the vertical distribution of larvae we established three experimental pycnocline treatments within clear plexiglass water columns (2.5cm x 2.5cm x 30cm): (1) ambient water (400ppm) in the top layer and acidic water in the bottom layer (1500ppm), (2) ambient water (400ppm) in both top and bottom layers, and (3) acidic water (1500ppm) in the top layer and ambient water (400ppm) in the bottom layer. Each water layer was 60-mL of water and filled the column 10-cm high, so when each experimental treatment was established it filled the column to 20-cm. We established the experimental treatments by increasing the density of seawater in the bottom layer by 0.003-0.005 g ml-1 using PercollTM GE Healthcare (Podolsky &amp; Emlet 1993). Experimental treatment water was kept at 12°C and pre-equilibrated to the desired pCO2 level and density. We also included blue food coloring (1 drop per 100-mL) to the dense bottom layer to more easily visualize the density layers while establishing experimental treatments. We set-up four replicate columns for each experimental treatment making twelve columns total per experiment.</p>
<p>&nbsp;On the day of each experiment, we incubated the experimental treatment columns in clear plexiglass water baths connected to a Fisher Scientific Isotemp recirculating water bath to maintain treatment temperature at 12°C throughout the experiment. We carefully injected 150 larvae by syringe into the bottom 2-cm of each column with no more than 2-mL of their culture water. Olympia oyster larvae are highly phototactic (personal observations), so larvae were kept in the dark and we video recorded their vertical positions under infrared light two times: the first time at 10 minutes of acclimation in the columns and the second time at 30 minutes of acclimation in the columns. To record, we used an infrared uEye camera equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand. We later counted by eye the number of larvae per centimeter area of each column from the videos.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- reformatted date from m/d/yy to yyyy-mm-dd<br />
- reduced precision of proportion_larvae from 9 to 3 decimal places</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
uEye video camera
uEye video camera
PI Supplied Instrument Name: uEye video camera PI Supplied Instrument Description:uEye camera equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
Fisher Scientific Isotemp Circulating Water Bath
Fisher Scientific Isotemp Circulating Water Bath
PI Supplied Instrument Name: Fisher Scientific Isotemp Circulating Water Bath PI Supplied Instrument Description:Used to maintain treatment temperature during experiment Instrument Name: In-situ incubator Instrument Short Name:in-situ incubator Instrument Description: A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/82/