http://lod.bco-dmo.org/id/dataset/753080 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2019-01-17 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) pH measurements from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017 2019-01-14 publication 2019-01-14 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2019-09-25 publication https://doi.org/10.1575/1912/bco-dmo.753080.1 Shawn M. Arellano Western Washington University principalInvestigator M. Brady Olson Western Washington University principalInvestigator Sylvia Yang Western Washington University principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Arellano, S., Olson, B., Yang, S. (2019) pH measurements from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-01-14 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.753080.1 [access date] Ostrea_pH_OA_Expt2017 Dataset Description: &lt;p&gt;This dataset contains pH measurements collected from a laboratory water column experiments to investigate the behavioral effects of ocean acidification on Olympia oyster larvae (&lt;em&gt;Ostrea lurida&lt;/em&gt;), July 2017.&lt;/p&gt; Methods and Sampling: &lt;p&gt;Collection &amp;amp; Larval Rearing&lt;/p&gt; &lt;p&gt;We collected adult Olympia oysters (&lt;em&gt;Ostrea &lt;/em&gt;&lt;em&gt;lurida&lt;/em&gt;) from Fidalgo Bay in June 2017 and maintained them in a sea table with continuous flowing seawater heated to 19-20°C at the Shannon Point Marine Center. We fed adult oysters were fed concentrated algae once a day (Shellfish Diet, Reed Mariculture) and utilized banjo-style filters (60-m) attached to the outflow pipes of the sea table to catch released &lt;em&gt;O. &lt;/em&gt;&lt;em&gt;lurida&lt;/em&gt; larvae. We then collected and reared larvae at 12°C in 3-L jars (2 individuals mL-1). Each jar of larvae received a 50% water change with 0.35-m filtered sea water and were fed Isochrysis galbana algae (50,000 cells mL-1) daily.&lt;/p&gt; &lt;p&gt;Experimental Design&lt;/p&gt; &lt;p&gt;To measure the effect of pH conditions on the vertical distribution of larvae we established three experimental pycnocline treatments within clear plexiglass water columns (2.5cm x 2.5cm x 30cm): (1) ambient water (400ppm) in the top layer and acidic water in the bottom layer (1500ppm), (2) ambient water (400ppm) in both top and bottom layers, and (3) acidic water (1500ppm) in the top layer and ambient water (400ppm) in the bottom layer. Each water layer was 60-mL of water and filled the column 10-cm high, so when each experimental treatment was established it filled the column to 20-cm. We established the experimental treatments by increasing the density of seawater in the bottom layer by 0.003-0.005 g ml-1 using dialyzed Percoll (Mills 1984; Podolskey &amp;amp; Emlet 1993). Experimental treatment water was kept at 12°C and pre-equilibrated to the desired pCO2 level and density. We also included blue food coloring (1 drop per 100-mL) to the dense bottom layer to more easily visualize the density layers while establishing experimental treatments. We set-up four replicate columns for each experimental treatment making twelve columns total per experiment.&lt;/p&gt; &lt;p&gt;On the day of each experiment, we incubated the experimental treatment columns in clear plexiglass water baths connected to a Fisher Scientific Isotemp recirculating water bath to maintain treatment temperature at 12°C throughout the experiment. We carefully injected 150 larvae by syringe into the bottom 2-cm of each column with no more than 2-mL of their culture water. Olympia oyster larvae are highly phototactic (personal observations), so we gave the larvae 10 minutes to acclimate in darkness and then recorded their vertical position in the water columns under infrared light. We video recorded the larvae’s vertical position in each column using an infrared uEye camera equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand. We later counted by eye the number of larvae per centimeter area of each column from the videos.&lt;/p&gt; &lt;p&gt;Sampling and analytical procedures:&lt;/p&gt; &lt;p&gt;Carefully collected water with a syringe and pipet from the top 1-3cm of the column, the bottom 1-3cm of the water column and right at the transition layer where the top and bottom layers of water met and was visible by the blue dye in the bottom layer of water. The water from the syringe was carefully transferred to a clean 2 ml microcentrifuge tube and pH was measured directly using a pH probe (Micro PerpHect Ross Ross® Combination pH electrode) and read with a Thermo Scientific Orion Star pH meter.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538626 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538626 completed Shawn M. Arellano Western Washington University 360-650-3634 Western Washington University 1900 Shannon Point Road Anacortes WA 98221 USA arellas@wwu.edu pointOfContact M. Brady Olson Western Washington University (360) 650-7400 1900 Shannon Point Rd. Anacortes WA 98221 United States Brady.Olson@wwu.edu pointOfContact Sylvia Yang Western Washington University 360-598-4460 SEA Discovery Center P.O. Box 2318 Poulsbo WA 98370 Sylvia.yang@wwu.edu pointOfContact asNeeded Dataset Version: 1 Unknown trial date column_name column_depth_cat column_depth_cm pH Thermo Scientific Orion Star A214 pH/ISE meter with a Micro PerpHect Ross® Combination pH electrode Fisher Scientific Isotemp Circulating Water Bath theme None, User defined experiment id date_local sample identification depth pH featureType BCO-DMO Standard Parameters Benchtop pH Meter In-situ incubator instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. RUI: Will climate change cause &#039;lazy larvae&#039;? Effects of climate stressors on larval behavior and dispersal https://osprey.bco-dmo.org/project/684167 RUI: Will climate change cause &#039;lazy larvae&#039;? Effects of climate stressors on larval behavior and dispersal <p>In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences</p> <p>As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.</p> Climate stressors on larvae largerWorkCitation project eng; USA biota oceans 2017-07-21 2017-08-03 Coastal Pacific, USA 0 BCO-DMO catalogue of parameters from pH measurements from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/753091.rdf Name: trial Units: unitless Description: <p>Trial number</p> http://lod.bco-dmo.org/id/dataset-parameter/753092.rdf Name: date Units: unitless Description: <p>Date of trial formatted as yyyy-mm-dd</p> http://lod.bco-dmo.org/id/dataset-parameter/753093.rdf Name: column_name Units: unitless Description: <p>Identifies the experimental water column treatment and replicate #: AN-# = Acidic water at the top and Neutral water at the bottom ; NN-# = Neutral water at the top and Neutral water at the bottom; NA-# = Neutral water at the top and Acid water at the bottom</p> http://lod.bco-dmo.org/id/dataset-parameter/753094.rdf Name: column_depth_cat Units: unitless Description: <p>Water column depth category where the pH sample was collected. top = 18-20 cm from bottom of water column; bottom = 1-2 cm from the bottom of the water column; transition point = middle of the water column where two treatment waters meet</p> http://lod.bco-dmo.org/id/dataset-parameter/753095.rdf Name: column_depth_cm Units: centimeters (cm) Description: <p>Water column depth in cm where the pH sample was collected.</p> http://lod.bco-dmo.org/id/dataset-parameter/753096.rdf Name: pH Units: standard pH units Description: <p>pH of seawater in water column</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2853 https://darchive.mblwhoilibrary.org/bitstream/1912/24620/1/dataset-753080_ostreaphoaexpt2017__v1.tsv download https://doi.org/10.1575/1912/bco-dmo.753080.1 download onLine dataset &lt;p&gt;Collection &amp;amp; Larval Rearing&lt;/p&gt; &lt;p&gt;We collected adult Olympia oysters (&lt;em&gt;Ostrea &lt;/em&gt;&lt;em&gt;lurida&lt;/em&gt;) from Fidalgo Bay in June 2017 and maintained them in a sea table with continuous flowing seawater heated to 19-20°C at the Shannon Point Marine Center. We fed adult oysters were fed concentrated algae once a day (Shellfish Diet, Reed Mariculture) and utilized banjo-style filters (60-m) attached to the outflow pipes of the sea table to catch released &lt;em&gt;O. &lt;/em&gt;&lt;em&gt;lurida&lt;/em&gt; larvae. We then collected and reared larvae at 12°C in 3-L jars (2 individuals mL-1). Each jar of larvae received a 50% water change with 0.35-m filtered sea water and were fed Isochrysis galbana algae (50,000 cells mL-1) daily.&lt;/p&gt; &lt;p&gt;Experimental Design&lt;/p&gt; &lt;p&gt;To measure the effect of pH conditions on the vertical distribution of larvae we established three experimental pycnocline treatments within clear plexiglass water columns (2.5cm x 2.5cm x 30cm): (1) ambient water (400ppm) in the top layer and acidic water in the bottom layer (1500ppm), (2) ambient water (400ppm) in both top and bottom layers, and (3) acidic water (1500ppm) in the top layer and ambient water (400ppm) in the bottom layer. Each water layer was 60-mL of water and filled the column 10-cm high, so when each experimental treatment was established it filled the column to 20-cm. We established the experimental treatments by increasing the density of seawater in the bottom layer by 0.003-0.005 g ml-1 using dialyzed Percoll (Mills 1984; Podolskey &amp;amp; Emlet 1993). Experimental treatment water was kept at 12°C and pre-equilibrated to the desired pCO2 level and density. We also included blue food coloring (1 drop per 100-mL) to the dense bottom layer to more easily visualize the density layers while establishing experimental treatments. We set-up four replicate columns for each experimental treatment making twelve columns total per experiment.&lt;/p&gt; &lt;p&gt;On the day of each experiment, we incubated the experimental treatment columns in clear plexiglass water baths connected to a Fisher Scientific Isotemp recirculating water bath to maintain treatment temperature at 12°C throughout the experiment. We carefully injected 150 larvae by syringe into the bottom 2-cm of each column with no more than 2-mL of their culture water. Olympia oyster larvae are highly phototactic (personal observations), so we gave the larvae 10 minutes to acclimate in darkness and then recorded their vertical position in the water columns under infrared light. We video recorded the larvae’s vertical position in each column using an infrared uEye camera equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand. We later counted by eye the number of larvae per centimeter area of each column from the videos.&lt;/p&gt; &lt;p&gt;Sampling and analytical procedures:&lt;/p&gt; &lt;p&gt;Carefully collected water with a syringe and pipet from the top 1-3cm of the column, the bottom 1-3cm of the water column and right at the transition layer where the top and bottom layers of water met and was visible by the blue dye in the bottom layer of water. The water from the syringe was carefully transferred to a clean 2 ml microcentrifuge tube and pH was measured directly using a pH probe (Micro PerpHect Ross Ross® Combination pH electrode) and read with a Thermo Scientific Orion Star pH meter.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;BCO-DMO Processing Notes:&lt;/strong&gt;&lt;br /&gt; - added conventional header with dataset name, PI name, version date&lt;br /&gt; - reformatted date from m/d/yy to yyyy-mm-dd&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Thermo Scientific Orion Star A214 pH/ISE meter with a Micro PerpHect Ross® Combination pH electrode Thermo Scientific Orion Star A214 pH/ISE meter with a Micro PerpHect Ross® Combination pH electrode PI Supplied Instrument Name: Thermo Scientific Orion Star A214 pH/ISE meter with a Micro PerpHect Ross® Combination pH electrode PI Supplied Instrument Description:The pH electrode was prepared before each set of measurements following instructions in the ROSS® Electrode User Guide (Thermo Fisher Scientific Inc.) and calibrated with a three-buffer calibration using Thermo ScientificTM OrionTM pH Buffer Individual Use Pouches. Instrument Name: Benchtop pH Meter Instrument Short Name:Benchtop pH Meter Instrument Description: An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms) This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications. Fisher Scientific Isotemp Circulating Water Bath Fisher Scientific Isotemp Circulating Water Bath PI Supplied Instrument Name: Fisher Scientific Isotemp Circulating Water Bath PI Supplied Instrument Description:Used to maintain treatment temperature during experiment Instrument Name: In-situ incubator Instrument Short Name:in-situ incubator Instrument Description: A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/82/