<div><p>The Environmental Sample Processor (ESP) filtered seawater sequentially through 0.2 um pore size polyethersulfone filters. Seawater was evacuated from filters and followed twice with a 2 minute incubation with 1 ml of RNAlater™. RNAlater was evacuated, and filters were stored in the ESP until they were transferred to -80 C upon instrument recovery. </p>
<p>Filters were processed for DNA using the phenol-chloroform extraction method of Crump et al. (2003) after placing the filters into 1 ml of DNA extraction buffer. Extracted DNA was sheared ultrasonically to ~350 bp fragments, and library preparation was performed at the Georgia Genomics and Bioinformatics Core (GGBC) facility. Single-end 250 bp sequencing was performed using an Illumina HiSeq Rapid Run at Hudson Alpha Genomic Services Laboratory (Huntsville, AL, USA).</p>
<p>Single-cell sequencing: Seawater was transferred directly from the Niskin bottle to a 50 ml Falcon tube and placed on ice until brought back to lab. Each sampling day, 3 x 1 ml of seawater was preserved in cryovials using 100 ul of glyTe (5 ml glycerol, 3 ml Milli-Q H2O, 1 ml 100 x TE pH 8.0, 0.2 um filter sterilized after mixing the above, and stored in -20 C freezer). Preserved samples were then placed in a -80 C freezer. Samples were processed and sequenced at Bigelow Single Cell Genomics Center (Stepanauskas and Sieracki, 2007).</p></div>
Metagenomic, metatranscriptomic, and single cell sequencing data collected during a deployment of the Environmental Sample Processor (ESP) in Fall, 2014 in Monterey Bay, CA
ESP 2014 Seq
<div><p><strong>BCO-DMO Processing notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- reformatted date from Mon-DD (Oct-04) to YYYYMMDD (20141004)</p></div>
753313
ESP 2014 Seq
2019-01-22T12:08:59-05:00
2019-01-22T12:08:59-05:00
2019-02-13T11:50:56-05:00
urn:bcodmo:dataset:753313
Metagenomic, and single cell sequencing data from a Fall 2014 ESP deployment in Monterey Bay, CA
false
Moran, M. Ann (2019) Metagenomic, and single cell sequencing data from a Fall 2014 ESP deployment in Monterey Bay, CA. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2019-01-22 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/753313 [access date]
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2019-01-22
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