http://lod.bco-dmo.org/id/dataset/753388
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-01-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway
2019-01-23
publication
2019-01-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-14
publication
https://doi.org/10.1575/1912/bco-dmo.753388.1
Elizabeth Harvey
Skidaway Institute of Oceanography
principalInvestigator
David Rowley
University of Rhode Island
principalInvestigator
Kristen E. Whalen
Haverford College
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Whalen, K., Rowley, D. (2019) Chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-01-23 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.753388.1 [access date]
HHQ Chlorophyll Dataset Description: <p>This dataset includes chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway. Microbial mesocosms were spiked with 2-heptyl-4-quinolone (HHQ).</p> Methods and Sampling: <p>Water samples for chlorophyll extraction were collected either from the mesocosms via a 5 L Niskin or subsampled from experimental bottles. Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in the dark at room temperature. After extraction, filters were removed from the sample, and the fluorescence of the sample was read on a Turner AU10. The sample was then acidified with 1 drop of 10% HCL and re-read on the same instrument. The fluorometer was calibrated prior to using with a chlorophyll standard purchased from Sigma.&nbsp;</p>
<p>Mesocosm treatment for all HHQ experiments was as follows:<br />
Redfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading</p>
<p>HHQ treatments here are as follows:<br />
High HHQ - 100 ng mL-1 (410 uM) added to triplicate 5L bottles.<br />
DMSO control - equivalent (v:v) DMSO added to triplicate 5L bottles.</p>
<p>&nbsp;All bottles were incubated for 24h in a flow-through tank, that was shaded to mimic in situ conditions. Chlorophyll samples were taken at T0 and T24 for all experiments.</p>
<p>Data were processed in Excel with statistics run in Excel, R, or Matlab.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1657898 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1657898
completed
Elizabeth Harvey
Skidaway Institute of Oceanography
NH
USA
elizabeth.harvey@unh.edu
pointOfContact
David Rowley
University of Rhode Island
4018749228
7 Greenhouse Road
Kingston
RI
028811967
US
drowley@uri.edu
pointOfContact
Kristen E. Whalen
Haverford College
610-795-6042
370 Lancaster Ave. KINSC S314
Haverford
PA
19041
USA
kwhalen1@haverford.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Sample
Experiment_num
Time
Replication
Volume_Filtered_mL
Extract_Volume_mL
Dilution_Factor
F_o
F_o_blank
F_a
F_a_blank
Total_chl_with_phaeo
Total_Chl_no_phaeo
Turner AU10 fluorometer
5 L Niskin
theme
None, User defined
date
sample identification
experiment id
incubation time or duration
replicate
volume of water filtered
volume
dilution
fluorescence
chl_tot
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Fluorometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Building a framework for the role of bacterial-derived chemical signals in mediating phytoplankton population dynamics
https://osprey.bco-dmo.org/project/709948
Collaborative Research: Building a framework for the role of bacterial-derived chemical signals in mediating phytoplankton population dynamics
<p><em>NSF Award Abstract:</em><br />
Bacteria and phytoplankton play a central role in the modification and flow of materials and nutrients through the marine environment. While it has been established that interactions between these two domains are complex, the mechanisms that underpin these interactions remain largely unknown. There is increasing recognition, however, that dissolved chemical cues govern these microbial interactions. This project focuses on establishing a mechanistic framework for how bacterially derived signaling molecules influence interactions between phytoplankton and bacteria. The quorum-sensing (QS) molecule, 2-heptyl-4-quinolone (HHQ) will be used as a model compound for these investigations. Previously published work suggests that exposure to very low levels of HHQ results in phytoplankton mortality. Gaining a mechanistic understanding of these ecologically important interactions will help to inform mathematical models for the accurate prediction of the cycling of material through the marine microbial loop. This work initiates a new, hybrid workshop-internship undergraduate research program in chemical ecology, with a focus</p>
<p>Bacteria and phytoplankton play a central role in the modification and flow of materials and nutrients through the marine environment. While it has been established that interactions between these two domains are complex, the mechanisms that underpin these interactions remain largely unknown. There is increasing recognition, however, that dissolved chemical cues govern these microbial interactions. This project focuses on establishing a mechanistic framework for how bacterially derived signaling molecules influence interactions between phytoplankton and bacteria. The quorum-sensing (QS) molecule, 2-heptyl-4-quinolone (HHQ) will be used as a model compound for these investigations. Previously published work suggests that exposure to very low levels of HHQ results in phytoplankton mortality. Gaining a mechanistic understanding of these ecologically important interactions will help to inform mathematical models for the accurate prediction of the cycling of material through the marine microbial loop. This work initiates a new, hybrid workshop-internship undergraduate research program in chemical ecology, with a focus into bacteria-phytoplankton interactions. Undergraduate students participate in an intense summer learning experience where research and field-based exercises are supplemented with short-lecture based modules. Students return to their home institutions and work closely with the PIs to conduct interdisciplinary research relating to the aims and scope of the summer research. This research also provides training and career development to two graduate students and a postdoctoral scientist.</p>
<p>Interactions between phytoplankton and bacteria play a central role in mediating biogeochemical cycling and microbial trophic structure in the ocean. The intricate relationships between these two domains of life are mediated via excreted molecules that facilitate communication and determine competitive outcomes. Despite their predicted importance, identifying these released compounds has remained a challenge. The PIs recently identified a bacterial QS molecule, HHQ, produced by globally distributed marine gamma-proteobacteria, which induces phytoplankton mortality. The PIs therefore hypothesize that bacteria QS signals are critical drivers of phytoplankton population dynamics and, ultimately, biogeochemical fluxes. This project investigates the timing and magnitude of HHQ production, and the physiological and transcriptomic responses of susceptible phytoplankton species to HHQ exposure, and quantifies the influence of HHQ on natural algal and bacterial assemblages. The work connects laboratory and field-based experiments to understand the governance of chemical signaling on marine microbial interactions, and has the potential to yield broadly applicable insights into how microbial interactions influence biogeochemical fluxes in the marine environment.</p>
HHQSignals
largerWorkCitation
project
eng; USA
oceans
5.281
5.281
60.221
60.221
2017-05-16
2017-05-31
Bergen, Norway
0
BCO-DMO catalogue of parameters from Chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/753402.rdf
Name: Date
Units: unitless
Description: <p>sampling date formatted as Mon dd yyyy</p>
http://lod.bco-dmo.org/id/dataset-parameter/753403.rdf
Name: Sample
Units: unitless
Description: <p>sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/753404.rdf
Name: Experiment_num
Units: unitless
Description: <p>experiment number</p>
http://lod.bco-dmo.org/id/dataset-parameter/753405.rdf
Name: Time
Units: hours
Description: <p>time since start of experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/753406.rdf
Name: Replication
Units: unitless
Description: <p>replicate number</p>
http://lod.bco-dmo.org/id/dataset-parameter/753407.rdf
Name: Volume_Filtered_mL
Units: milliliters (mL)
Description: <p>volume filtered</p>
http://lod.bco-dmo.org/id/dataset-parameter/753408.rdf
Name: Extract_Volume_mL
Units: milliliters (mL)
Description: <p>volume extracted</p>
http://lod.bco-dmo.org/id/dataset-parameter/753409.rdf
Name: Dilution_Factor
Units: unitless
Description: <p>dilution factor</p>
http://lod.bco-dmo.org/id/dataset-parameter/753410.rdf
Name: F_o
Units: Relative Fluorescence Units (RFU)
Description: <p>initial fluorescence reading</p>
http://lod.bco-dmo.org/id/dataset-parameter/753411.rdf
Name: F_o_blank
Units: Relative Fluorescence Units (RFU)
Description: <p>initial fluorescence of control blank</p>
http://lod.bco-dmo.org/id/dataset-parameter/753412.rdf
Name: F_a
Units: Relative Fluorescence Units (RFU)
Description: <p>fluorescence after acidification</p>
http://lod.bco-dmo.org/id/dataset-parameter/753413.rdf
Name: F_a_blank
Units: Relative Fluorescence Units (RFU)
Description: <p>fluorescence of control blank after acidification</p>
http://lod.bco-dmo.org/id/dataset-parameter/753414.rdf
Name: Total_chl_with_phaeo
Units: micrograms chlorophyll/Liter (ug/L)
Description: <p>total chlorophyll including phaeophytin</p>
http://lod.bco-dmo.org/id/dataset-parameter/753415.rdf
Name: Total_Chl_no_phaeo
Units: micrograms chlorophyll/Liter (ug/L)
Description: <p>total chlorophyll NOT including phaeophytin</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4883
https://darchive.mblwhoilibrary.org/bitstream/1912/23805/1/dataset-753388_hhq-chlorophyll__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.753388.1
download
onLine
dataset
<p>Water samples for chlorophyll extraction were collected either from the mesocosms via a 5 L Niskin or subsampled from experimental bottles. Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in the dark at room temperature. After extraction, filters were removed from the sample, and the fluorescence of the sample was read on a Turner AU10. The sample was then acidified with 1 drop of 10% HCL and re-read on the same instrument. The fluorometer was calibrated prior to using with a chlorophyll standard purchased from Sigma.&nbsp;</p>
<p>Mesocosm treatment for all HHQ experiments was as follows:<br />
Redfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading</p>
<p>HHQ treatments here are as follows:<br />
High HHQ - 100 ng mL-1 (410 uM) added to triplicate 5L bottles.<br />
DMSO control - equivalent (v:v) DMSO added to triplicate 5L bottles.</p>
<p>&nbsp;All bottles were incubated for 24h in a flow-through tank, that was shaded to mimic in situ conditions. Chlorophyll samples were taken at T0 and T24 for all experiments.</p>
<p>Data were processed in Excel with statistics run in Excel, R, or Matlab.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- reduced precision of total chlorophyll columns from (5 to 15) to 2 decimal places</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Turner AU10 fluorometer
Turner AU10 fluorometer
PI Supplied Instrument Name: Turner AU10 fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
5 L Niskin
5 L Niskin
PI Supplied Instrument Name: 5 L Niskin PI Supplied Instrument Description:Used to collect water samples. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/