In situ instruments deployed at 1 m depth below the surface and as per: Tait, Z. S., M. Thompson, and A. Stubbins (2015) Chemical fouling reduction of a submersible steel spectrophotometer in estuarine environments using a sacrificial zinc anode. Journal of Environmental Quality. doi: 10.2134/jeq2014.11.0484

Samples for laboratory analyses were collected using a refrigerated autosampler (ISCO) which pumped samples from a depth of 1 m below the water surface. Samples were returned to the laboratory and filtered through 0.2 micron Polycap filters within 24 hours of collection.

DOC and TDN using a Shimadzu TOC/TDN analyzer as per automated setup in Stubbins, A., and Dittmar, T. (2012) Low volume quantification of dissolved organic carbon and dissolved nitrogen. Limnology and Oceanography: Methods. doi: 10.4319/lom.2012.10.347.

Lignin via cupric oxide oxidation and GC-MS as per: Spencer, R.G.M., Hernes, P.J., Ruf, R., Baker, A., Dyda, R.Y., Stubbins, A., and Six, J. (2010) Temporal controls on dissolved organic matter and lignin biogeochemistry in a pristine tropical river. Journal of Geophysical Research – Biogeosciences. doi:10.1029/2009JG001180.

Dissolved black carbon by nitric acid oxidation to BPCAs followed by HPLC with spectrophotometric detection as per the non-isotopic method in Wagner, S., Brandes, J., Goranov, A.I., Drake, T. W., Spencer, R. G. W., Stubbins, A. (2017) Online quantification and compound-specific stable isotopic analysis of black carbon in environmental matrices via liquid chromatography-isotope ratio mass spectrometry. Limnology and Oceanography: Methods. doi: 10.1002/lom3.10219.
Nutrients were analyzed using a nutrient analyzer as per: Bittar, T. B., S. A. Berger, L. M. Birsa, T. L. Walters, M. E. Thompson, R. G. M. Spencer, E. L. Mann, A. Stubbins, M. E. Frischer, and J. A. Brandes (2016) Seasonal dynamics of dissolved, particulate and microbial components of a tidal saltmarsh-dominated estuary under contrasting levels of freshwater discharge. Estuarine, Coastal and Shelf Science. doi: 10.1016/j.ecss.2016.08.046.