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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/754661.rdf" xlink:actuate="onRequest">Coral (Porites rus) calcification and chemistry data from outdoor flumes at the UCB Gump Research Station Moorea, French Polynesia in April and March of 2012</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Carpenter, R., Edmunds, P. J., Comeau, S. (2020) Coral (Porites rus) calcification and chemistry data from outdoor flumes at the UCB Gump Research Station Moorea, French Polynesia in April and March of 2012. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-11-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.754661.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Methodology:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Calcification was estimated by measuring the change in buoyant weight (Davies 1989) based on an initial measurement of all the corals and subsequent measures on one-third of the corals after 1, 2, and 3 weeks of incubation in order to monitor the evolution of the treatment effects through time. The difference between initial and final buoyant weight was converted to dry weight increments using an aragonite density of 2.93 g cm-3 (Davies 1989) and standardized to the area of the corals as determined by the aluminum foil technique (Marsh 1970). Dry tissue weight of the organisms also was measured to normalize calcification to biomass to evaluate changes in biomass that might result from changes in the size of energy reserves attributed to the feeding regimes. To determine tissue dry weight, corals were fixed in 10 % formalin solution for 48 h, then the skeleton was dissolved by immersion in 5 % HCl that was replaced daily until the skeleton was dissolved (2–4 days). Tissues were rinsed in distilled water and dried for 48 h at 60 °C prior to weighing (±1 mg) and normalizing to area (mg cm-2).&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
pH was measured using an open-cell autotitrator (Model T50, Mettler-Toledo) calibrated every other day with Tris buffer provided by Dr. Andrew Dickson (Scripps Institution of Oceanography). Total alkalinity (AT) and salinity were measured daily during the first half of the incubation, and then every other day during the second half of the experiment based on the rationale that conditions were demonstrably stable. Seawater analyses were performed on the day of sampling using open-cell potentiometric titration with an automatic titrator (T50, Mettler-Toledo). Measurements were taken on 50-mL samples at *23 °C, and AT calculated after Dickson et al. (2007). Prior to each set of AT measurements, titrations of certified reference material (batch 108) provided by Dr. A. Dickson were performed and yielded values that were ±3 lmol kg-1 of certified values. Parameters of the carbonate system were calculated from salinity, temperature, AT, and pHT using the R package seacarb (Lavigne and Gattuso 2011).&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
See Comeau et al. (2013) for more details.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/54987.rdf" xlink:title="OCE-0417412" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0417412 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0417412</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/55110.rdf" xlink:title="OCE-1041270" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1041270 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1041270</gmx:Anchor>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/520630.rdf" xlink:title="OCE-1026851" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1026851 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1026851</gmx:Anchor>
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                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/program/2030.rdf" xlink:title="Program Name" xlink:actuate="onRequest">Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)</gmx:Anchor>
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                            <gco:CharacterString>NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels &quot;Hang On&quot; Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)</gco:CharacterString>
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                            <gco:CharacterString>&lt;p&gt;&lt;strong&gt;NSF Award Abstract: &lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;Coral reefs provide important benefits to society, from food to exceptional biodiversity to shoreline protection and recreation, but they are threatened by natural perturbations and human activities, including those causing global-scale changes. These pressures increasingly are causing coral reefs to undergo large, often abrupt, ecological changes where corals are being replaced by seaweeds or other undesirable organisms. Historically, the major agent of disturbance to coral reefs has been powerful storms, but in recent decades, episodes of mass coral bleaching from marine heat waves have become more frequent and severe as the temperature of ocean surface waters continues to rise. Coral reefs are further stressed by local human activities that cause nutrient pollution and deplete herbivorous fishes that control growth of seaweeds. Studying how coral reefs respond to these two types of disturbance under different levels of nutrient pollution and fishing provides essential information on what affects the ability of coral reefs to buffer environmental change and disturbances without collapsing to a persistent, degraded condition. The fundamental goals of the Moorea Coral Reef Long Term Ecological Research program (MCR LTER) are to understand how and why coral reefs change over time, to assess the consequences of these changes, and to contribute scientific knowledge needed to sustain coral reef ecosystems and the important societal services they provide. This research improves understanding and management of coral reefs, which benefits all groups concerned with the welfare of this ecologically, economically and culturally important ecosystem. In addition to academic communities, scientific findings are communicated to interested individuals, non-governmental organizations, island communities and governmental entities. These findings also are integrated into K-12, undergraduate, graduate and public education activities through a multi-pronged program that includes inquiry-based curricula, interactive and media-based public education programs, and internet-based resources. MCR?s research, training, education and outreach efforts all emphasize broadening participation in STEM fields and strengthening STEM literacy.&lt;/p&gt;
&lt;p&gt;New research activities build on MCR LTER?s powerful foundation of long-term observations and broad ecological understanding of oceanic coral reefs to address the following core issues: How is the changing disturbance regime (recurrent heat waves in addition to cyclonic storms) altering the resilience of coral reefs, and what are the ecological consequences of altered resilience? Research activities are organized around a unifying framework that explicitly addresses how reef communities are affected by the nature and history of coral-killing disturbances, and how those responses to disturbance are influenced by the pattern of local human stressors. New studies answer three focal questions: (1) How do different disturbance types, which either remove (storms) or retain (heat waves) dead coral skeletons, affect community dynamics, abrupt changes in ecological state, and resilience? (2) How do local stressors interact with new disturbance regimes to create spatial heterogeneity in community dynamics, ecosystem processes, and spatial resilience? And (3) What attributes of coral and coral reef communities influence their capacity to remain resilient under current and future environmental conditions? These questions provide an unparalleled opportunity to test hypotheses and advance theory regarding ecological resilience and the causes and consequences of abrupt ecological change, which is broadly relevant across aquatic and terrestrial ecosystems.&lt;/p&gt;
&lt;p&gt;This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;From &lt;a href=&quot;http://www.lternet.edu/sites/mcr/&quot;&gt;http://www.lternet.edu/sites/mcr/&lt;/a&gt; and &lt;a href=&quot;http://mcr.lternet.edu/&quot;&gt;http://mcr.lternet.edu/&lt;/a&gt;&lt;/strong&gt;:&lt;br /&gt;
The Moorea Coral Reef LTER site encompasses the coral reef complex that surrounds the island of Moorea, French Polynesia (17°30'S, 149°50'W). Moorea is a small, triangular volcanic island 20 km west of Tahiti in the Society Islands of French Polynesia. An offshore barrier reef forms a system of shallow (mean depth ~ 5-7 m), narrow (~0.8-1.5 km wide) lagoons around the 60 km perimeter of Moorea. All major coral reef types (e.g., fringing reef, lagoon patch reefs, back reef, barrier reef and fore reef) are present and accessible by small boat.&lt;/p&gt;
&lt;p&gt;The MCR LTER was established in 2004 by the US National Science Foundation (NSF) and is a partnership between the University of California Santa Barbara and California State University, Northridge. MCR researchers include marine scientists from the UC Santa Barbara, CSU Northridge, UC Davis, UC Santa Cruz, UC San Diego, CSU San Marcos, Duke University and the University of Hawaii. Field operations are conducted from the UC Berkeley Richard B. Gump South Pacific Research Station on the island of Moorea, French Polynesia.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;MCR LTER Data:&lt;/strong&gt; The Moorea Coral Reef (MCR) LTER data are managed by and available directly from the MCR project data site URL shown above.  The datasets listed below were collected at or near the MCR LTER sampling locations, and funded by NSF OCE as ancillary projects related to the MCR LTER core research themes.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;This project is supported by continuing grants with slight name variations:&lt;/strong&gt;&lt;/p&gt;
&lt;ul&gt;
&lt;li&gt;LTER: Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
&lt;li&gt;LTER: MCR II - Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
&lt;li&gt;LTER: MCR IIB: Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
&lt;li&gt;LTER: MCR III: Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
&lt;li&gt;LTER: MCR IV: Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
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                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/2242.rdf" xlink:title="Project Name" xlink:actuate="onRequest">RUI: Ocean Acidification- Category 1- The effects of ocean acidification on the organismic biology and community ecology of corals, calcified algae, and coral reefs</gmx:Anchor>
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                            <gco:CharacterString>&lt;p&gt;While coral reefs have undergone unprecedented changes in community structure in the past 50 y, they now may be exposed to their gravest threat since the Triassic. This threat is increasing atmospheric CO2, which equilibrates with seawater and causes ocean acidification (OA). In the marine environment, the resulting decline in carbonate saturation state (Omega) makes it energetically less feasible for calcifying taxa to mineralize; this is a major concern for coral reefs. It is possible that the scleractinian architects of reefs will cease to exist as a mineralized taxon within a century, and that calcifying algae will be severely impaired. While there is a rush to understand these effects and make recommendations leading to their mitigation, these efforts are influenced strongly by the notion that the impacts of pCO2 (which causes Omega to change) on calcifying taxa, and the mechanisms that drive them, are well-known. The investigators believe that many of the key processes of mineralization on reefs that are potentially affected by OA are only poorly known and that current knowledge is inadequate to support the scaling of OA effects to the community level. It is vital to measure organismal-scale calcification of key taxa, elucidate the mechanistic bases of these responses, evaluate community scale calcification, and finally, to conduct focused experiments to describe the functional relationships between these scales of mineralization.&lt;/p&gt;
&lt;p&gt;This project is a 4-y effort focused on the effects of Ocean Acidification (OA) on coral reefs at multiple spatial and functional scales. The project focuses on the corals, calcified algae, and coral reefs of Moorea, French Polynesia, establishes baseline community-wide calcification data for the detection of OA effects on a decadal-scale, and builds on the research context and climate change focus of the Moorea Coral Reef LTER.&lt;/p&gt;
&lt;p&gt;This project is a hypothesis-driven approach to compare the effects of OA on reef taxa and coral reefs in Moorea. The PIs will utilize microcosms to address the impacts and mechanisms of OA on biological processes, as well as the ecological processes shaping community structure. Additionally, studies of reef-wide metabolism will be used to evaluate the impacts of OA on intact reef ecosystems, to provide a context within which the experimental investigations can be scaled to the real world, and critically, to provide a much needed reference against which future changes can be gauged.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Datasets listed in the &quot;Dataset Collection&quot; section include references to results journal publications published as part of this project.&lt;/strong&gt;&lt;/p&gt;</gco:CharacterString>
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	Name: CO2_1
	Units: umol/kg
	Description: &lt;p&gt;Carbon dioxide. Calculated using seacarb (Lavigne and Gattuso, 2011).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832576.rdf
	Name: pCO2water_SST_wet_1
	Units: uatm
	Description: &lt;p&gt;Partial pressure of carbon dioxide (water) at sea surface temperature (wet air). Calculated using seacarb (Lavigne and Gattuso, 2011).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832577.rdf
	Name: fCO2water_SST_wet_1
	Units: uatm
	Description: &lt;p&gt;Fugacity of carbon dioxide (water) at sea surface temperature (wet air). Calculated using seacarb (Lavigne and Gattuso, 2011).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832578.rdf
	Name: Bicarbonate_ion_1
	Units: umol/kg
	Description: &lt;p&gt;Bicarbonate ion [HCO3]-. Calculated using seacarb (Lavigne and Gattuso, 2011).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832579.rdf
	Name: Carbonate_ion_1
	Units: umol/kg
	Description: &lt;p&gt;Carbonate ion [CO3]2-. Calculated using seacarb (Lavigne and Gattuso, 2011).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832580.rdf
	Name: DIC_1
	Units: umol/kg
	Description: &lt;p&gt;Carbon, inorganic, dissolved. Calculated using seacarb (Lavigne and Gattuso, 2011).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832581.rdf
	Name: AT
	Units: umol/kg
	Description: &lt;p&gt;Alkalinity, total.  Potentiometric titration.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832582.rdf
	Name: Omega_Arg_1
	Units: omega aragonite (Ωa)
	Description: &lt;p&gt;Aragonite saturation state. Calculated using seacarb (Lavigne and Gattuso, 2011).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832583.rdf
	Name: Omega_Cal_1
	Units: omega calcite (Ωcal)
	Description: &lt;p&gt;Calcite saturation state. Calculated using seacarb (Lavigne and Gattuso, 2011).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832584.rdf
	Name: CSC_flag
	Units: unitless
	Description: &lt;p&gt;Carbonate system computation flag&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832585.rdf
	Name: CO2_2
	Units: umol/kg
	Description: &lt;p&gt;Carbon dioxide. Calculated using seacarb (Lavigne and Gattuso, 2011) after Nisumaa et al. (2010).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832586.rdf
	Name: pCO2water_SST_wet_2
	Units: uatm
	Description: &lt;p&gt;Partial pressure of carbon dioxide (water) at sea surface temperature (wet air). Calculated using seacarb (Lavigne and Gattuso, 2011) after Nisumaa et al. (2010).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832587.rdf
	Name: fCO2water_SST_wet_2
	Units: uatm
	Description: &lt;p&gt;Fugacity of carbon dioxide (water) at sea surface temperature (wet air). Calculated using seacarb (Lavigne and Gattuso, 2011) after Nisumaa et al. (2010).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832588.rdf
	Name: Bicarbonate_ion_2
	Units: umol/kg
	Description: &lt;p&gt;Bicarbonate ion [HCO3]-. Calculated using seacarb (Lavigne and Gattuso, 2011) after Nisumaa et al. (2010).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832589.rdf
	Name: Carbonate_ion_2
	Units: umol/kg
	Description: &lt;p&gt;Carbonate ion [CO3]2-. Calculated using seacarb (Lavigne and Gattuso, 2011) after Nisumaa et al. (2010).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832590.rdf
	Name: DIC_2
	Units: umol/kg
	Description: &lt;p&gt;Carbon, inorganic, dissolved (DIC). Calculated using seacarb (Lavigne and Gattuso, 2011) after Nisumaa et al. (2010).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832591.rdf
	Name: Omega_Arg_2
	Units: omega aragonite (Ωa)
	Description: &lt;p&gt;Aragonite saturation state. Calculated using seacarb (Lavigne and Gattuso, 2011) after Nisumaa et al. (2010).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/832592.rdf
	Name: Omega_Cal_2
	Units: omega calcite (Ωcal)
	Description: &lt;p&gt;Calcite saturation state. Calculated using seacarb (Lavigne and Gattuso, 2011) after Nisumaa et al. (2010).&lt;/p&gt; 
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            <gco:CharacterString>PI Supplied Instrument Name: ThermoFisher Traceable Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor   Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
