http://lod.bco-dmo.org/id/dataset/755923
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-02-18
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Water geochemistry from surface samples collected along the Neuse River, Neuse River Estuary, and Pamlico Sound in North Carolina between October and December 2016, following the passage of Hurricane Matthew
2019-02-18
publication
2019-02-18
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-15
publication
https://doi.org/10.1575/1912/bco-dmo.755923.1
Chris Osburn
North Carolina State University - Marine, Earth and Atmospheric Sciences
principalInvestigator
Hans Paerl
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Osburn, C., Paerl, H. (2019) Water geochemistry from surface samples collected along the Neuse River, Neuse River Estuary, and Pamlico Sound in North Carolina between October and December 2016, following the passage of Hurricane Matthew. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-02-18 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.755923.1 [access date]
Water geochemistry from surface samples collected along the Neuse River, Neuse River Estuary, and Pamlico Sound in North Carolina between October and December 2016, following the passage of Hurricane Matthew Dataset Description: Methods and Sampling: <p>Raw water surface samples were collected at sites along the Neuse River (NR), Neuse River Estuary (NRE), and Pamlico Sound (PS) in North Carolina over a period of three months, between October and December 2016, following the passage of Hurricane Matthew on October 9, 2016. Sampling was conducted approximately weekly across the NR, NRE, and PS sites. Riverine samples were collected, in 1 L brown HDPE bottles, from bridge overpasses along the main-stem of the NR at USGS gaged locations. Estuarine and sound samples were collected, in 1 L opaque HDPE bottles and frozen for shipment to NCSU, by the Neuse River Estuary Modeling and Monitoring Project ("ModMon") located at the University of North Carolina at Chapel Hill's Institute of Marine Science (UNC-IMS) in Morehead City, NC. Collection bottles were cleansed in a detergent bath, rinsed profusely with Milli-Q ultrapure water, and left to air dry before sampling. We conducted additional sampling in an area of freshwater riparian wetlands between the last riverine site on the NR near Fort Barnwell, NC and first estuarine site on the NRE at the Street's Ferry Bridge crossing near Vanceboro, NC in March and October 2017.</p>
<p>Raw water samples were thawed at room temperature, and a known volume of water was filtered through pre-combusted (at 45C for 5 hours) 0.7 m Whatman glass fiber filters (GF/F) via vacuum. Prior to sample filtration, 150 mL of Milli-Q water was used to rinse each filter. The filtrate was collected into 60 mL polycarbonate bottles (detergent-washed and rinsed thoroughly with Milli-Q water) for optical analyses and 40 mL amber-tinted borosilicate glass vials (detergent-washed, rinsed thoroughly with Milli-Q water, and combusted at 45C for 5 hours) for dissolved organic carbon (DOC) concentration and stable carbon isotope (13C-DOC) analyses. Filtrate was stored at 4C until optical measurements were made, generally within 48 hours. Filtrate for DOC analysis was acidified to a pH of 2 with 85% phosphoric acid (H<sub>3</sub>PO<sub>4</sub>), then stored at 4C until measurements were completed. GF/F filters were stored at -20C until particulate organic carbon (POC) concentration and stable carbon isotopes (13C-POC) could be measured.</p>
<p>Absorbance measurements were made with a Varian Cary 300UV spectrophotometer in 1 cm quartz cuvettes, over a range of wavelengths (200-800 nm), and then blank corrected using Milli-Q ultrapure water. (Osburn and Morris, 2003)</p>
<p>Fluorescence was measured on a Varian Eclipse spectrofluorometer under the following conditions: excitation (Ex) wavelengths from 240 to 450 nm at 5 nm intervals, emission (Em) wavelengths from 300 to 600 nm at 2 nm intervals. The fluorescence data was concatenated into excitation-emission matrices (EEMs), which display qualitative information about OM within a system, such as the sources of organic matter (Coble, 2007).</p>
<p>Samples for DOC were immediately filtered through 0.2 um polyethersulfone filters into glass vials, capped with Teflon-lined septa and stored without headspace at 4C until analysis. DIC concentration ([DIC]) and delta-13C-DIC value was measured on a OI Analytical TOC analyzer (Doctor et al., 2008)</p>
<p>To quantify DOC concentration, [DOC], samples were first sparged with ultrapure Argon gas for 20 minutes to remove dissolved inorganic carbon (DIC). Sparged samples were analyzed on an OI Analytical 1030D Aurora total organic carbon analyzer, using wet chemical oxidation, coupled to a Thermo Delta V Plus isotope ratio mass spectrometer (IRMS) to determine stable isotope values, expressed as the standard delta notation, 13C-DOC (Osburn and St. Jean, 2007). [DOC] were blank-corrected for ultra-pure Milli-Q water, then calculated using a linear regression curve of known caffeine standards with concentrations from 1 to 20 mg C per L. 13C-DOC values were blank corrected and referenced to the Vienna Pee Dee Belemnite (VPDB) scale via a linear regression of six caffeine (IAEA-600, -27.7 +/- 0.04‰) and two sucrose (IAEA-C6, -10.8 +/- 0.03‰) International Atomic Energy Agency (IAEA) standards. Precision for [DOC] and 13C-DOC values were +/- 0.4‰ based on reproducibility and calibration to sucrose standard. Milli-Q blanks were ran every 10 samples for quality control and confidence of instrument.</p>
<p>Frozen GF/F filters were thawed, then dried at 60C for at least 24 hours in an oven to prepare for measurement of particulate carbon and nitrogen concentrations ([POC] and [PN] respectively) and the stable isotope of [POC], 13C-POC. Analysis was conducted on a Thermo Finnigan Flash Series 1112 Elemental Analyzer (EA), coupled to a Delta V IRMS via a Conflo III interface. Calibration curves were made using acetanilide, L-glutamic acid (USGS-40), caffeine (IAEA-600), oxalic acid (NIST-4990C), and sucrose (IAEA-C6) standards. Weighed standards (0.200-0.600 mg) were placed in low blank tin capsules (4 x 6mm), while dried GF/F filters were folded then placed in larger low blank tin capsules (10 x 12mm). Carbon and nitrogen peak locations on the EA, [POC], and [PN] were determined using acetanilide standards with percent content values of 71.09% and 10.36% for carbon and nitrogen, respectively. 13C-POC for each sample was determined by converting the measured value to the VPDB scale using a linear fit regression of the L-glutamic acid (-26.39‰), caffeine (-27.77‰), oxalic acid (-17.8‰), and sucrose (-10.8‰) standards. Similar to the precision for [DOC] and 13C-DOC, 13C-POC values were +/- 0.4‰ based on reproducibility and calibration to known standards.</p>
<p>Synoptic measurements were collected using a handheld YSI probe and include salinity, pH, temperature, dissolved oxygen concentration (DO), DO saturation (%DO), Total Dissolved Solids (TDS), and Specific Conductivity (Spec. Cond.).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1706009 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1706009
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1705972 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1705972
completed
Chris Osburn
North Carolina State University - Marine, Earth and Atmospheric Sciences
919.515.0382
Marine, Earth, and Atmospheric Sciences Dept. 2800 Faucette Drive
Raleigh
NC
27695
USA
closburn@ncsu.edu
pointOfContact
Hans Paerl
University of North Carolina at Chapel Hill
252-726-6841
3431 Arendell Street
Morehead City
NC
28557
US
hans_paerl@unc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_ID
Site_Location
Year
Month
Day
Time
Timezone
Lat
Lon
Temp
Salinity
GFF_IW
GFF_FW
GFF_Diff
River_Dis
Spec_Con
DO
DO_Sat
pH
TDS
VF
TSS
POC
PN
CDOM
DIC
DOC
d13C_DOC
d13C_POC
d15N_PN
C_to_N
SUVA254
S275_295
S350_400
SR
Max_Fl_Em
Max_Fl
B
T
A
C
M
N
FI
BIX
HIX
T_to_B_peak
T_to_M_peak
T_to_N_peak
T_to_C_peak
A_to_T_peak
A_to_C_peak
A_to_M_peak
M_to_C_peak
C_to_N_peak
Thermo Scientific FlashEA 1112 Nitrogen and Carbon analyzer
Cary Eclipse Spectrofluorometer
Thermo Scientific Delta V Isotope Ratio Mass Spectrometer
Cary UV 300 Spectrophotometer
OI Analytical Aurora 1030 W Total Organic Carbon (TOC) Analyzer
YSI Pro Plus multiparameter meter
theme
None, User defined
sample identification
site
year
month of year
day of month
time of day
time_zone
latitude
longitude
water temperature at measurement depth
salinity
weight
No BCO-DMO term
specific conductance
dissolved Oxygen
O2 saturation
pH
volume of water filtered
particulate organic Carbon (POC)
Nitrogen
absorption coefficient
dissolved inorganic Carbon
dissolved organic Carbon
d13C
d15N
Carbon to Nitrogen ratio (C:N)
absorbance
fluorescence
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Fluorometer
Isotope-ratio Mass Spectrometer
Spectrophotometer
Total Organic Carbon Analyzer
YSI Professional Plus Multi-Parameter Probe
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RAPID: Collaborative Research: Carbon and nutrient responses in an estuarine-coastal complex impacted by floodwaters from Hurricane Matthew
https://osprey.bco-dmo.org/project/734599
RAPID: Collaborative Research: Carbon and nutrient responses in an estuarine-coastal complex impacted by floodwaters from Hurricane Matthew
<p>NSF abstract:</p>
<p>This project constitutes a rapid-response effort to investigate the effects of the extreme flooding event of Hurricane Matthew on coastal cycling of carbon and nutrient elements. The investigators aim to improve our understanding of how estuaries and coastal systems respond to extreme events by measuring carbon, nitrogen, and phosphorus loading into the Neuse River Estuary-Pamlico Sound (NRE-PS) coastal ecosystem. The NRE-PS is the urgent study site to examine resulting effects of Hurricane Matthew on coastal environments because it is downstream of the most intense flooding that occurred, and it is located on the North Atlantic "hurricane track", likely to be impacted by future extreme events. Furthermore, it is the focus of a long-term monitoring program in that system, the Neuse River Estuary Modeling and Monitoring Program (ModMon), which was initiated in 1993. That date is significant because it preceded a recent rise in Atlantic tropical cyclone activity and has been able to capture the biogeochemical and ecological effects of major storms that have impacted the NC coast, including Hurricanes Fran (1996), Floyd (1999), Isabel (2003), and Irene (2011). Incorporating intensive sampling of the Hurricane Matthew flooding in the context of these ongoing observations will enable a comparison of the material fluxes into this coastal environment resulting from a major tropical storm and provide a comparison with other coastal environments. Outcomes of this work will be communicated widely via existing outreach efforts of each investigator's research programs to local and regional resource managers, regulators, and other stakeholders. Nutrient loading will be critical information for stakeholders because the Neuse Estuary is an EPA 303(d) listed waterway impaired for nitrogen. Project results can aid in understanding the effects of extreme weather events on ecosystem disturbances and inform coastal carbon flux estimates.</p>
<p>Specific questions to be addressed in the context of this project include: 1) How do coastal carbon and nitrogen budgets respond to floodwaters from tropical storms and hurricanes? 2) What is the biological and photochemical reactivity of this material? The first question can be answered via a relatively short, yet intense, period of observations, such as is proposed here. The second question, which has potential teleconnections to climate in terms of carbon fluxes, food web responses, and other ecosystems processes, requires a longer duration study. However, Question 2 could be answered by further study of samples collected during a short and intense period of sampling proposed to answer Question 1. Work proposed in this project will result in estimates of fluxes and reservoirs of key constituents such as dissolved inorganic carbon (DIC), dissolved and particulate organic carbon and nitrogen (DOC, POC, DON, PON), and N and P nutrients, as well as chlorophyll biomass and pigment analyses.</p>
HMATT
largerWorkCitation
project
eng; USA
oceans
-78.43603
-76.2006
34.94888
35.675008
2016-10-12
2017-10-17
Coastal waters of eastern North Carolina (Pamlico Sound) 35.09 N 76.52 W
0
BCO-DMO catalogue of parameters from Water geochemistry from surface samples collected along the Neuse River, Neuse River Estuary, and Pamlico Sound in North Carolina between October and December 2016, following the passage of Hurricane Matthew
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/755989.rdf
Name: Sample_ID
Units: unitless
Description: <p>Osburn Lab Sample Identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/755990.rdf
Name: Site_Location
Units: unitless
Description: <p>Project Site Identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/755991.rdf
Name: Year
Units: unitless
Description: <p>4-digit year of Sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/755992.rdf
Name: Month
Units: unitless
Description: <p>2-digit month of Sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/755993.rdf
Name: Day
Units: unitless
Description: <p>2-digit day of Sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/755994.rdf
Name: Time
Units: unitless
Description: <p>Local Time of Sample (EST); fomrat: HH:MM:SS</p>
http://lod.bco-dmo.org/id/dataset-parameter/755995.rdf
Name: Timezone
Units: unitless
Description: <p>Time zone (UTC miunus 5 hours)</p>
http://lod.bco-dmo.org/id/dataset-parameter/755996.rdf
Name: Lat
Units: Decimal degrees
Description: <p>Latitude of Sample Location; positive values = North</p>
http://lod.bco-dmo.org/id/dataset-parameter/755997.rdf
Name: Lon
Units: Decimal degrees
Description: <p>Longitude of Sample Location; positive values = East</p>
http://lod.bco-dmo.org/id/dataset-parameter/755998.rdf
Name: Temp
Units: Degrees Celsius
Description: <p>Water Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/755999.rdf
Name: Salinity
Units: PSU
Description: <p>Salinity of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/756000.rdf
Name: GFF_IW
Units: Milligrams (mg)
Description: <p>Initial Weight of Glass Fiber Filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/756001.rdf
Name: GFF_FW
Units: Milligrams (mg)
Description: <p>Final Weight of Glass Fiber Filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/756002.rdf
Name: GFF_Diff
Units: Milligrams (mg)
Description: <p>The difference in Weight of the Glass Fiber Filters</p>
http://lod.bco-dmo.org/id/dataset-parameter/756003.rdf
Name: River_Dis
Units: Cubic meters per second (m^3/s)
Description: <p>River Discharge taken from USGS gaging sites along the Neuse River</p>
http://lod.bco-dmo.org/id/dataset-parameter/756004.rdf
Name: Spec_Con
Units: Microsiemens per Centimeter (uS/cm)
Description: <p>Specific Conductance of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/756005.rdf
Name: DO
Units: Milligrams per Liter (mg/L)
Description: <p>Dissolved Oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/756006.rdf
Name: DO_Sat
Units: Percentage
Description: <p>Dissolved Oxygen Saturation</p>
http://lod.bco-dmo.org/id/dataset-parameter/756007.rdf
Name: pH
Units: unitless
Description: <p>pH of the Sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/756008.rdf
Name: TDS
Units: Milligrams per Liter (mg/L)
Description: <p>Total Dissolved Solids</p>
http://lod.bco-dmo.org/id/dataset-parameter/756009.rdf
Name: VF
Units: Milliliters (mL)
Description: <p>Volume of water Filtered through GF/F</p>
http://lod.bco-dmo.org/id/dataset-parameter/756010.rdf
Name: TSS
Units: Milligrams per Liter (mg/L)
Description: <p>Total Suspended Solids (Difference of Weighed Filter/Volume Filtered)</p>
http://lod.bco-dmo.org/id/dataset-parameter/756011.rdf
Name: POC
Units: Micrograms per Liter (ug/L)
Description: <p>Particulate Organic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/756012.rdf
Name: PN
Units: Micrograms per Liter (ug/L)
Description: <p>Particulate Nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/756013.rdf
Name: CDOM
Units: 1/meters
Description: <p>Absorbance coefficent of sample at 254 nanometers</p>
http://lod.bco-dmo.org/id/dataset-parameter/756014.rdf
Name: DIC
Units: Milligrams per Liter (mg/L)
Description: <p>Dissolved Inorganic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/756015.rdf
Name: DOC
Units: Milligrams per Liter (mg/L)
Description: <p>Dissolved Organic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/756016.rdf
Name: d13C_DOC
Units: Permille (‰)
Description: <p>Carbon Stable Isotope Ratio of Dissolved Organic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/756017.rdf
Name: d13C_POC
Units: Permille (‰)
Description: <p>Carbon Stable Isotope Ratio of Particulate Organic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/756018.rdf
Name: d15N_PN
Units: Permille (‰)
Description: <p>Nitrogen Stable Isotope Ratio of Particulate Nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/756019.rdf
Name: C_to_N
Units: unitless
Description: <p>Atomic Carbon to Nitrogen Ratio</p>
http://lod.bco-dmo.org/id/dataset-parameter/756020.rdf
Name: SUVA254
Units: Liters per Milligram Carbon per Meter (L/(mgC m))
Description: <p>Specific Ultraviolet Absorbance at 254 nanometers</p>
http://lod.bco-dmo.org/id/dataset-parameter/756021.rdf
Name: S275_295
Units: 1/nm
Description: <p>Spectral Slope from 275 to 295 nanometers</p>
http://lod.bco-dmo.org/id/dataset-parameter/756022.rdf
Name: S350_400
Units: 1/nm
Description: <p>Spectral Slope from 350 to 400 nanometers</p>
http://lod.bco-dmo.org/id/dataset-parameter/756023.rdf
Name: SR
Units: unitless
Description: <p>Slope Ratio (S275-295:S350-400)</p>
http://lod.bco-dmo.org/id/dataset-parameter/756024.rdf
Name: Max_Fl_Em
Units: Nanometers (nm)
Description: <p>Fluorescence Emission Maximum</p>
http://lod.bco-dmo.org/id/dataset-parameter/756025.rdf
Name: Max_Fl
Units: QSE
Description: <p>Maximum Fluorescence Value</p>
http://lod.bco-dmo.org/id/dataset-parameter/756026.rdf
Name: B
Units: QSE
Description: <p>Tyrosine-like, Protein-like Fluorescence peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756027.rdf
Name: T
Units: QSE
Description: <p>Tryptophan-like, Protein-like Fluorescence peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756028.rdf
Name: A
Units: QSE
Description: <p>Humic-like Fluorescence peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756029.rdf
Name: C
Units: QSE
Description: <p>Humic-like Fluorescence peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756030.rdf
Name: M
Units: QSE
Description: <p>Marine Humic-like Fluorescence peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756031.rdf
Name: N
Units: QSE
Description: <p>Unknown fluorescence peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756032.rdf
Name: FI
Units: unitless
Description: <p>Fluorescence Index</p>
http://lod.bco-dmo.org/id/dataset-parameter/756033.rdf
Name: BIX
Units: unitless
Description: <p>Biological Index</p>
http://lod.bco-dmo.org/id/dataset-parameter/756034.rdf
Name: HIX
Units: unitless
Description: <p>Humic Index</p>
http://lod.bco-dmo.org/id/dataset-parameter/756035.rdf
Name: T_to_B_peak
Units: unitless
Description: <p>Ratio of T peak to B peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756036.rdf
Name: T_to_M_peak
Units: unitless
Description: <p>Ratio of T peak to M peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756037.rdf
Name: T_to_N_peak
Units: unitless
Description: <p>Ratio of T peak to N peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756038.rdf
Name: T_to_C_peak
Units: unitless
Description: <p>Ratio of T peak to C peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756039.rdf
Name: A_to_T_peak
Units: unitless
Description: <p>Ratio of A peak to T peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756040.rdf
Name: A_to_C_peak
Units: unitless
Description: <p>Ratio of A peak to C peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756041.rdf
Name: A_to_M_peak
Units: unitless
Description: <p>Ratio of A peak to M peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756042.rdf
Name: M_to_C_peak
Units: unitless
Description: <p>Ratio of M peak to C peak</p>
http://lod.bco-dmo.org/id/dataset-parameter/756043.rdf
Name: C_to_N_peak
Units: unitless
Description: <p>Ratio of C peak to N peak</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
112528
https://darchive.mblwhoilibrary.org/bitstream/1912/23842/1/dataset-755923_hurricane-matthew-geochemical-data__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.755923.1
download
onLine
dataset
<p>Raw water surface samples were collected at sites along the Neuse River (NR), Neuse River Estuary (NRE), and Pamlico Sound (PS) in North Carolina over a period of three months, between October and December 2016, following the passage of Hurricane Matthew on October 9, 2016. Sampling was conducted approximately weekly across the NR, NRE, and PS sites. Riverine samples were collected, in 1 L brown HDPE bottles, from bridge overpasses along the main-stem of the NR at USGS gaged locations. Estuarine and sound samples were collected, in 1 L opaque HDPE bottles and frozen for shipment to NCSU, by the Neuse River Estuary Modeling and Monitoring Project ("ModMon") located at the University of North Carolina at Chapel Hill's Institute of Marine Science (UNC-IMS) in Morehead City, NC. Collection bottles were cleansed in a detergent bath, rinsed profusely with Milli-Q ultrapure water, and left to air dry before sampling. We conducted additional sampling in an area of freshwater riparian wetlands between the last riverine site on the NR near Fort Barnwell, NC and first estuarine site on the NRE at the Street's Ferry Bridge crossing near Vanceboro, NC in March and October 2017.</p>
<p>Raw water samples were thawed at room temperature, and a known volume of water was filtered through pre-combusted (at 45C for 5 hours) 0.7 m Whatman glass fiber filters (GF/F) via vacuum. Prior to sample filtration, 150 mL of Milli-Q water was used to rinse each filter. The filtrate was collected into 60 mL polycarbonate bottles (detergent-washed and rinsed thoroughly with Milli-Q water) for optical analyses and 40 mL amber-tinted borosilicate glass vials (detergent-washed, rinsed thoroughly with Milli-Q water, and combusted at 45C for 5 hours) for dissolved organic carbon (DOC) concentration and stable carbon isotope (13C-DOC) analyses. Filtrate was stored at 4C until optical measurements were made, generally within 48 hours. Filtrate for DOC analysis was acidified to a pH of 2 with 85% phosphoric acid (H<sub>3</sub>PO<sub>4</sub>), then stored at 4C until measurements were completed. GF/F filters were stored at -20C until particulate organic carbon (POC) concentration and stable carbon isotopes (13C-POC) could be measured.</p>
<p>Absorbance measurements were made with a Varian Cary 300UV spectrophotometer in 1 cm quartz cuvettes, over a range of wavelengths (200-800 nm), and then blank corrected using Milli-Q ultrapure water. (Osburn and Morris, 2003)</p>
<p>Fluorescence was measured on a Varian Eclipse spectrofluorometer under the following conditions: excitation (Ex) wavelengths from 240 to 450 nm at 5 nm intervals, emission (Em) wavelengths from 300 to 600 nm at 2 nm intervals. The fluorescence data was concatenated into excitation-emission matrices (EEMs), which display qualitative information about OM within a system, such as the sources of organic matter (Coble, 2007).</p>
<p>Samples for DOC were immediately filtered through 0.2 um polyethersulfone filters into glass vials, capped with Teflon-lined septa and stored without headspace at 4C until analysis. DIC concentration ([DIC]) and delta-13C-DIC value was measured on a OI Analytical TOC analyzer (Doctor et al., 2008)</p>
<p>To quantify DOC concentration, [DOC], samples were first sparged with ultrapure Argon gas for 20 minutes to remove dissolved inorganic carbon (DIC). Sparged samples were analyzed on an OI Analytical 1030D Aurora total organic carbon analyzer, using wet chemical oxidation, coupled to a Thermo Delta V Plus isotope ratio mass spectrometer (IRMS) to determine stable isotope values, expressed as the standard delta notation, 13C-DOC (Osburn and St. Jean, 2007). [DOC] were blank-corrected for ultra-pure Milli-Q water, then calculated using a linear regression curve of known caffeine standards with concentrations from 1 to 20 mg C per L. 13C-DOC values were blank corrected and referenced to the Vienna Pee Dee Belemnite (VPDB) scale via a linear regression of six caffeine (IAEA-600, -27.7 +/- 0.04‰) and two sucrose (IAEA-C6, -10.8 +/- 0.03‰) International Atomic Energy Agency (IAEA) standards. Precision for [DOC] and 13C-DOC values were +/- 0.4‰ based on reproducibility and calibration to sucrose standard. Milli-Q blanks were ran every 10 samples for quality control and confidence of instrument.</p>
<p>Frozen GF/F filters were thawed, then dried at 60C for at least 24 hours in an oven to prepare for measurement of particulate carbon and nitrogen concentrations ([POC] and [PN] respectively) and the stable isotope of [POC], 13C-POC. Analysis was conducted on a Thermo Finnigan Flash Series 1112 Elemental Analyzer (EA), coupled to a Delta V IRMS via a Conflo III interface. Calibration curves were made using acetanilide, L-glutamic acid (USGS-40), caffeine (IAEA-600), oxalic acid (NIST-4990C), and sucrose (IAEA-C6) standards. Weighed standards (0.200-0.600 mg) were placed in low blank tin capsules (4 x 6mm), while dried GF/F filters were folded then placed in larger low blank tin capsules (10 x 12mm). Carbon and nitrogen peak locations on the EA, [POC], and [PN] were determined using acetanilide standards with percent content values of 71.09% and 10.36% for carbon and nitrogen, respectively. 13C-POC for each sample was determined by converting the measured value to the VPDB scale using a linear fit regression of the L-glutamic acid (-26.39‰), caffeine (-27.77‰), oxalic acid (-17.8‰), and sucrose (-10.8‰) standards. Similar to the precision for [DOC] and 13C-DOC, 13C-POC values were +/- 0.4‰ based on reproducibility and calibration to known standards.</p>
<p>Synoptic measurements were collected using a handheld YSI probe and include salinity, pH, temperature, dissolved oxygen concentration (DO), DO saturation (%DO), Total Dissolved Solids (TDS), and Specific Conductivity (Spec. Cond.).</p>
Specified by the Principal Investigator(s)
<p>Data Processing: Excel used for spreadsheets; Matlab (v 2016 to 2018) is used to process absorbance and fluorescence results and data and to conduct statistical testing. SigmaPlot is used for data visualization.&nbsp;</p>
<p>Problem Report:&nbsp;Some gaps in data occur when sample sites were inaccessible or when samples were lost during shipment or during analysis.</p>
<p>BCO-DMO Processing: modified parameter names (removed special characters;&nbsp;replaced spaces and hyphens with underscores; added "peak" to peak ratios for clarity).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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508-289-2009
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Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
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Thermo Scientific FlashEA 1112 Nitrogen and Carbon analyzer
Thermo Scientific FlashEA 1112 Nitrogen and Carbon analyzer
PI Supplied Instrument Name: Thermo Scientific FlashEA 1112 Nitrogen and Carbon analyzer PI Supplied Instrument Description:POC & PN. Calibrated with acetanilide. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Cary Eclipse Spectrofluorometer
Cary Eclipse Spectrofluorometer
PI Supplied Instrument Name: Cary Eclipse Spectrofluorometer PI Supplied Instrument Description:DOM Fluorescence. Calibrated with quinine sulfate solution after normalization to instrument’s water Raman peak. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Thermo Scientific Delta V Isotope Ratio Mass Spectrometer
Thermo Scientific Delta V Isotope Ratio Mass Spectrometer
PI Supplied Instrument Name: Thermo Scientific Delta V Isotope Ratio Mass Spectrometer PI Supplied Instrument Description:13C-DOC, 13C-POC and 15N-PN. The isotope-ratio mass spectrometer (IRMS) allows the precise measurement of mixtures of naturally occurring isotopes. Reference CO2 and N2 gases are calibrated to NIST- or IAEA-traceable standards of known isotopic composition. Seawater DOC is calibrated with the Hansell Deep Sea Reference. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Cary UV 300 Spectrophotometer
Cary UV 300 Spectrophotometer
PI Supplied Instrument Name: Cary UV 300 Spectrophotometer PI Supplied Instrument Description:DOM absorbance. Instrument checks performed regularly. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
OI Analytical Aurora 1030 W Total Organic Carbon (TOC) Analyzer
OI Analytical Aurora 1030 W Total Organic Carbon (TOC) Analyzer
PI Supplied Instrument Name: OI Analytical Aurora 1030 W Total Organic Carbon (TOC) Analyzer PI Supplied Instrument Description:DOC & DIC. Calibrated with stock solutions of sodium bicarbonate (DIC) and caffeine (DOC). A certified reference material (CRM) for DIC is analyzed regularly as a check standard. Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
YSI Pro Plus multiparameter meter
YSI Pro Plus multiparameter meter
PI Supplied Instrument Name: YSI Pro Plus multiparameter meter PI Supplied Instrument Description:Temp, Salinity, pH, DO, DO%, TDS, Spec. Cond. Sensors calibrated with reference solutions prior to each use. DO and pH sensors are replaced and new sensors calibrated approximately every year. Instrument Name: YSI Professional Plus Multi-Parameter Probe Instrument Short Name:YSI ProPlus Instrument Description: The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.