http://lod.bco-dmo.org/id/dataset/756413
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-02-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Environmental data from Niskin bottle sampling during the Fall 2016 ESP deployment in Monterey Bay, CA
2019-11-08
publication
2019-11-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-04-17
publication
https://doi.org/10.1575/1912/bco-dmo.756413.1
Mary Ann Moran
University of Georgia
principalInvestigator
Ronald P. Kiene
Dauphin Island Sea Lab
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Moran, M. A., Kiene, R. (2019) Environmental data from Niskin bottle sampling during the Fall 2016 ESP deployment in Monterey Bay, CA. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2019-11-08 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.756413.2 [access date]
Environmental data from Niskin bottle sampling during the Fall 2016 ESP deployment in Monterey Bay, CA Dataset Description: Methods and Sampling: <p>Grab samples were taken using Niskin bottles that collected seawater at the same depth and location of the Environmental Sample Processor deployed at Station M0 (36.835 N, 121.901W). Water was transferred to a low-density polyethylene cubitainer and maintained at ambient temperature until return to lab within 30 min.</p>
<p>Chlorophyll a: 150 ml of seawater was filtered through a 25 mm GF/F filter in triplicate using a vacuum pump and &lt;5 in Hg pressure. The filter was placed in a glass scintillation vial and 10 ml of 90% acetone was added and placed in -20 freezer for at least 24 hours to extract the pigment. Extracted chlorophyll <em>a</em> was quantified using fluorometry (Pennington and Chavez, 2000).</p>
<p>Flow Cytometry: Cubitainer seawater was transferred to a 50 ml Falcon tube using laminar flow. 1.8 ml was then aliquoted to triplicate cryovials and preserved with 200 ul of 5% glutaraldehyde and stored at -80 degrees C. Analysis was run on a Beckman Coulter Altra flow cytometer for detection of DNA, pigments, and forward and side light scatter (Monger and Landry, 1993).</p>
<p>Akashiwo Microscopy Counts: 7 - 14 ml of seawater was preserved to 1% final concentration electron microscopy grade glutaraldehyde and stored at 4 degrees C. Slides were made by filtering the full volume onto a 0.22 um black polycarbonate filter (GE Water &amp; Process Technologies) using a vacuum pump (&lt;5 in Hg), and cells were counted under epifluorescence microscopy.</p>
<p>DMSP concentrations: Immediately upon return to the deck, duplicate samples were collected from the Niskin bottle for in situ dissolved DMSP (DMSPd) (see details below) before seawater transfer to the cubitainer. Upon return to the laboratory, the cubitainer of water was gently mixed by inversion and three replicate 10 ml sub-samples were removed by pipette into individual 15 ml centrifuge tubes (Corning, polypropylene). The samples were immediately acidified with 0.3 ml of 50% concentrated HCl (1.5% final concentration of concentrated HCl) to preserve total DMSP (dissolved plus particulate). These DMSPt samples were closed tightly and stored until analysis (described below) which took place within three months of collection.&nbsp;</p>
<p>DMSPd consumption: To measure the consumption rate of dissolved DMSP, we used the glycine betaine (GBT) inhibition technique (Kiene &amp; Gerard, 1995; Li et al., 2016). Immediately upon return to the laboratory, six 500 ml glass bottles were filled with seawater from the gently-mixed cubitainer. Three of the bottles were treated with 25 ul of a 100 mM GBT anhydrous reagent (Sigma) solution (10 uM final GBT concentration), and three were left untreated as controls. Bottles were incubated in seawater maintained within 1 degree C of the in situ temperature. Immediately after GBT addition, the first time point was collected by simultaneously filtering ~50 ml sub-samples from each bottle through 47 mm Whatman GF/F filters using the small volume gravity drip filtration protocol of Kiene and Slezak (2006). The first&nbsp;3.5 ml of filtrate from each sample was collected into 15 ml centrifuge tubes (Corning, polypropylene) that contained 100 ul of 50% HCl to immediately preserve any DMSP passing through the GF/F filter, which is defined as dissolved DMSP (DMSPd). Additional time points from each bottle were collected at 3 and 6 h. The rate of change of DMSPd in no-treatment bottles was subtracted from the rate of change in the +GBT bottles to obtain an estimate of DMSPd consumption rate (Kiene and Gerard, 1995).</p>
<p>&lt; 5 µm DMSPd: The DMSP that was less than 5 µm was measured in water from the cubitainer in the lab, using the drip filtration protocol, as described above for DMSPd, except that a 5.0 µm pore size, track-etched polycarbonate filter was used for the filtration.</p>
<p>DMSP Analysis: DMSP was quantified by proxy as the amount of DMS released from samples after alkaline cleavage (White, 1982). For DMSPt, 0.05 to 0.5 ml of each preserved sample was pipetted into a 14 ml glass serum vial, with the volume being adjusted based on the concentration of DMSPt in the sample. For DMSPd, the volume pipetted was 1.0 to 3.0 ml. Each serum vial was treated with 1 ml of 5 M NaOH and capped with a Teflon-faced serum stopper (Wheaton). After 1 h, the amount of DMS in each vial was quantified by purge and trap gas chromatography with flame photometric detection. Briefly, each vial was attached to the purge system and a flow of helium (90-100 ml per minute) allowed bubbling of the solution. An excurrent needle led to a Nafion dryer and six-port valve (Valco). The DMS in the samples was cryotrapped in a Teflon tubing loop immersed in liquid nitrogen. After a 4 min sparge, during which &gt;99% of the DMS in the samples was removed, hot water replaced the liquid nitrogen to introduce the DMS into the Shimadzu GC-2014 gas chromatograph. Separation of the sulfur gases was achieved with a Chromosil 330 column (Supelco; Sigma) maintained at 60 degrees C with a helium carrier flow of 25 ml per minute. The flame photometric detector was operated in sulfur mode and maintained at 175 degrees C. Minimum detection limits during this study were 0.5 to 1 pmol DMS per sample with minimum detectable concentrations ranging from 0.17 to 10 nM, depending on the volume analyzed. The GC-FPD system was calibrated with a gas stream containing known amounts of DMS from a permeation system.</p>
<p>Problem report:&nbsp;For November chlorophyll a<em> </em>samples, fluorescence after acid addition not measured but estimated from samples with similar total fluorescence (Pennington and Chavez, 2000).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1342694 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1342694
completed
Mary Ann Moran
University of Georgia
706-542-6481
Department of Marine Sciences
Athens
GA
30602
USA
mmoran@uga.edu
pointOfContact
Ronald P. Kiene
Dauphin Island Sea Lab
pointOfContact
asNeeded
Dataset Version: 2
Unknown
Date
Time_Pacific
ISO_DateTime_Local
Depth
Chlorophyll_a
DMSPd_in_situ
DMSPd_lab
DMSPt
picophotosynthetic_eukaryotes
nanophotosynthetic_eukaryotes
microphotosynthetic_eukaryotes_group_1
microphotosynthetic_eukaryotes_group_2
DMSPd_consumption_rate
lt_5_um_DMSPd
Photosynthetic_eukaryotes
Heterotrophic_bacteria
Synechococcus
Akashiwo
Chlorophyll_a_stdev
DMSPd_in_situ_stdev
DMSPd_lab_stdev
DMSPt_stdev
picophotosynthetic_eukaryotes_stdev
nanophotosynthetic_eukaryotes_stdev
microphotosynthetic_eukaryotes_group_1_stdev
microphotosynthetic_eukaryotes_group_2_stdev
lt_5_um_DMSPd_stdev
Photosynthetic_eukaryotes_stdev
Heterotrophic_bacteria_stdev
Synechococcus_stdev
ISO_DateTime_UTC
Beckman Coulter Altra
Shimadzu GC-2014 gas chromatograph
Turner Designs 10-AU Fluorometer
theme
None, User defined
date
time of day
ISO_DateTime_Local
depth
chlorophyll a
dimethylsulphoniopropionate concentration
count
heterotrophic bacteria abundance from flow cytometry
synechococcus abundance
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
Flow Cytometer
Gas Chromatograph
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
Moran_Monterey_2016
service
Deployment Activity
Monterey Bay, CA, USA; Station M0
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dimensions of Biodiversity
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446
Dimensions of Biodiversity
(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program. [MORE from NSF]
The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.
Dimensions of Biodiversity
largerWorkCitation
program
Bacterial Taxa that Control Sulfur Flux from the Ocean to the Atmosphere
https://osprey.bco-dmo.org/project/541255
Bacterial Taxa that Control Sulfur Flux from the Ocean to the Atmosphere
<p>Surface ocean bacterioplankton preside over a divergence point in the marine sulfur cycle where the fate of dimethylsulfoniopropionate (DMSP) is determined. While it is well recognized that this juncture influences the fate of sulfur in the ocean and atmosphere, its regulation by bacterioplankton is not yet understood. Based on recent findings in biogeochemistry, bacterial physiology, bacterial genetics, and ocean instrumentation, the microbial oceanography community is poised to make major advances in knowledge of this control point. This research project is ascertaining how the major taxa of bacterial DMSP degraders in seawater regulate DMSP transformations, and addresses the implications of bacterial functional, genetic, and taxonomic diversity for global sulfur cycling.</p>
<p>The project is founded on the globally important function of bacterial transformation of the ubiquitous organic sulfur compound DMSP in ocean surface waters. Recent genetic discoveries have identified key genes in the two major DMSP degradation pathways, and the stage is now set to identify the factors that regulate gene expression to favor one or the other pathway during DMSP processing. The taxonomy of the bacteria mediating DMSP cycling has been deduced from genomic and metagenomic sequencing surveys to include four major groups of surface ocean bacterioplankton. How regulation of DMSP degradation differs among these groups and maps to phylogeny in co-occurring members is key information for understanding the marine sulfur cycle and predicting its function in a changing ocean. Using model organism studies, microcosm experiments (at Dauphin Island Sea Lab, AL), and time-series field studies with an autonomous sample collection instrument (at Monterey Bay, CA), this project is taking a taxon-specific approach to decipher the regulation of bacterial DMSP degradation.</p>
<p>This research addresses fundamental questions of how the diversity of microbial life influences the geochemical environment of the oceans and atmosphere, linking the genetic basis of metabolic potential to taxonomic diversity. The project is training graduate students and post-doctoral scholars in microbial biodiversity and providing research opportunities and mentoring for undergraduate students. An outreach program is enhance understanding of the role and diversity of marine microorganisms in global elemental cycles among high school students. Advanced Placement Biology students are participating in marine microbial research that covers key learning goals in the AP Biology curriculum. Two high school students are selected each year for summer research internships in PI laboratories.</p>
OceanSulfurFluxBact
largerWorkCitation
project
eng; USA
oceans
Monterey Bay, CA, USA; Station M0
-121.901
-121.901
36.835
36.835
2016-09-23
2016-11-08
0
BCO-DMO catalogue of parameters from Environmental data from Niskin bottle sampling during the Fall 2016 ESP deployment in Monterey Bay, CA
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/781308.rdf
Name: Date
Units: unitless
Description: <p>Date. Format: yyyy-mm-dd.</p>
http://lod.bco-dmo.org/id/dataset-parameter/781309.rdf
Name: Time_Pacific
Units: unitless
Description: <p>Time (Pacific time zone). Format: HH:MM.</p>
http://lod.bco-dmo.org/id/dataset-parameter/781310.rdf
Name: ISO_DateTime_Local
Units: unitless
Description: <p>Date and time (local) formatted to ISO8601 standard.</p>
http://lod.bco-dmo.org/id/dataset-parameter/781311.rdf
Name: Depth
Units: meters (m)
Description: <p>Sampling depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/781312.rdf
Name: Chlorophyll_a
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyll a</p>
http://lod.bco-dmo.org/id/dataset-parameter/781313.rdf
Name: DMSPd_in_situ
Units: nanomolar (nM)
Description: <p>Dissolved DMSP sampled on boat immediately after seawater collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/781314.rdf
Name: DMSPd_lab
Units: nanomolar (nM)
Description: <p>Dissolved DMSP sampled after seawater transferred to lab</p>
http://lod.bco-dmo.org/id/dataset-parameter/781315.rdf
Name: DMSPt
Units: nanomolar (nM)
Description: <p>Total DMSP</p>
http://lod.bco-dmo.org/id/dataset-parameter/781316.rdf
Name: picophotosynthetic_eukaryotes
Units: cells per milliliter (cells/mL)
Description: <p>Determined by flow cytometry; relative estimate of size; cells with lowest scatter signatures</p>
http://lod.bco-dmo.org/id/dataset-parameter/781317.rdf
Name: nanophotosynthetic_eukaryotes
Units: cells per milliliter (cells/mL)
Description: <p>Determined by flow cytometry; relative estimate of size; cells with intermediate scatter signatures</p>
http://lod.bco-dmo.org/id/dataset-parameter/781318.rdf
Name: microphotosynthetic_eukaryotes_group_1
Units: cells per milliliter (cells/mL)
Description: <p>Determined by flow cytometry; relative estimate of size; cells with highest scatter and chlorophyll signatures</p>
http://lod.bco-dmo.org/id/dataset-parameter/781319.rdf
Name: microphotosynthetic_eukaryotes_group_2
Units: cells per milliliter (cells/mL)
Description: <p>Determined by flow cytometry; relative estimate of size; cells with highest scatter and a bit lower chlorophyll signatures</p>
http://lod.bco-dmo.org/id/dataset-parameter/781320.rdf
Name: DMSPd_consumption_rate
Units: nM/d
Description: <p>Dissolved DMSP consumption rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/781321.rdf
Name: lt_5_um_DMSPd
Units: nanomolar (nM)
Description: <p>Dissolved DMSP concentration of seawater filtered through 5 µm filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/781322.rdf
Name: Photosynthetic_eukaryotes
Units: cells per milliliter (cells/mL)
Description: <p>Determined by flow cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/781323.rdf
Name: Heterotrophic_bacteria
Units: cells per milliliter (cells/mL)
Description: <p>Determined by flow cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/781324.rdf
Name: Synechococcus
Units: cells per milliliter (cells/mL)
Description: <p>Determined by flow cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/781325.rdf
Name: Akashiwo
Units: cells per milliliter (cells/mL)
Description: <p>Determined by microscopy</p>
http://lod.bco-dmo.org/id/dataset-parameter/781326.rdf
Name: Chlorophyll_a_stdev
Units: micrograms per liter (ug/L)
Description: <p>Standard deviation of Chlorophyll_a (n = 3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781327.rdf
Name: DMSPd_in_situ_stdev
Units: nanomolar (nM)
Description: <p>Standard deviation of DMSPd_in_situ (n = 3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781328.rdf
Name: DMSPd_lab_stdev
Units: nanomolar (nM)
Description: <p>Standard deviation of DMSPd_lab (n =3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781329.rdf
Name: DMSPt_stdev
Units: nanomolar (nM)
Description: <p>Standard deviation of DMSPt (n = 3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781330.rdf
Name: picophotosynthetic_eukaryotes_stdev
Units: cells per milliliter (cells/mL)
Description: <p>Standard deviation of picophotosynthetic_eukaryotes (n = 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781331.rdf
Name: nanophotosynthetic_eukaryotes_stdev
Units: cells per milliliter (cells/mL)
Description: <p>Standard deviation of nanophotosynthetic_eukaryotes (n = 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781332.rdf
Name: microphotosynthetic_eukaryotes_group_1_stdev
Units: cells per milliliter (cells/mL)
Description: <p>Standard deviation of microphotosynthetic_eukaryotes_group_1 (n = 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781333.rdf
Name: microphotosynthetic_eukaryotes_group_2_stdev
Units: cells per milliliter (cells/mL)
Description: <p>Standard deviation of microphotosynthetic_eukaryotes_group_2 (n = 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781334.rdf
Name: lt_5_um_DMSPd_stdev
Units: nanomolar (nM)
Description: <p>Standard deviation of lt_5_um_DMSPd (n = 3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781335.rdf
Name: Photosynthetic_eukaryotes_stdev
Units: cells per milliliter (cells/mL)
Description: <p>Standard deviation of Photosynthetic_eukaryotes (n = 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781336.rdf
Name: Heterotrophic_bacteria_stdev
Units: cells per milliliter (cells/mL)
Description: <p>Standard deviation of Heterotrophic_bacteria (n = 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781337.rdf
Name: Synechococcus_stdev
Units: cells per milliliter (cells/mL)
Description: <p>Standard deviation of Synechococcus (n = 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/781338.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time (converted to UTC) formatted to ISO8601 standard.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6699
https://darchive.mblwhoilibrary.org/bitstream/1912/24793/1/dataset-756413_niskin-2016__v2.tsv
download
4341
https://darchive.mblwhoilibrary.org/bitstream/1912/24029/1/dataset-756413_niskin-2016__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.756413.1
download
onLine
dataset
<p>Grab samples were taken using Niskin bottles that collected seawater at the same depth and location of the Environmental Sample Processor deployed at Station M0 (36.835 N, 121.901W). Water was transferred to a low-density polyethylene cubitainer and maintained at ambient temperature until return to lab within 30 min.</p>
<p>Chlorophyll a: 150 ml of seawater was filtered through a 25 mm GF/F filter in triplicate using a vacuum pump and &lt;5 in Hg pressure. The filter was placed in a glass scintillation vial and 10 ml of 90% acetone was added and placed in -20 freezer for at least 24 hours to extract the pigment. Extracted chlorophyll <em>a</em> was quantified using fluorometry (Pennington and Chavez, 2000).</p>
<p>Flow Cytometry: Cubitainer seawater was transferred to a 50 ml Falcon tube using laminar flow. 1.8 ml was then aliquoted to triplicate cryovials and preserved with 200 ul of 5% glutaraldehyde and stored at -80 degrees C. Analysis was run on a Beckman Coulter Altra flow cytometer for detection of DNA, pigments, and forward and side light scatter (Monger and Landry, 1993).</p>
<p>Akashiwo Microscopy Counts: 7 - 14 ml of seawater was preserved to 1% final concentration electron microscopy grade glutaraldehyde and stored at 4 degrees C. Slides were made by filtering the full volume onto a 0.22 um black polycarbonate filter (GE Water &amp; Process Technologies) using a vacuum pump (&lt;5 in Hg), and cells were counted under epifluorescence microscopy.</p>
<p>DMSP concentrations: Immediately upon return to the deck, duplicate samples were collected from the Niskin bottle for in situ dissolved DMSP (DMSPd) (see details below) before seawater transfer to the cubitainer. Upon return to the laboratory, the cubitainer of water was gently mixed by inversion and three replicate 10 ml sub-samples were removed by pipette into individual 15 ml centrifuge tubes (Corning, polypropylene). The samples were immediately acidified with 0.3 ml of 50% concentrated HCl (1.5% final concentration of concentrated HCl) to preserve total DMSP (dissolved plus particulate). These DMSPt samples were closed tightly and stored until analysis (described below) which took place within three months of collection.&nbsp;</p>
<p>DMSPd consumption: To measure the consumption rate of dissolved DMSP, we used the glycine betaine (GBT) inhibition technique (Kiene &amp; Gerard, 1995; Li et al., 2016). Immediately upon return to the laboratory, six 500 ml glass bottles were filled with seawater from the gently-mixed cubitainer. Three of the bottles were treated with 25 ul of a 100 mM GBT anhydrous reagent (Sigma) solution (10 uM final GBT concentration), and three were left untreated as controls. Bottles were incubated in seawater maintained within 1 degree C of the in situ temperature. Immediately after GBT addition, the first time point was collected by simultaneously filtering ~50 ml sub-samples from each bottle through 47 mm Whatman GF/F filters using the small volume gravity drip filtration protocol of Kiene and Slezak (2006). The first&nbsp;3.5 ml of filtrate from each sample was collected into 15 ml centrifuge tubes (Corning, polypropylene) that contained 100 ul of 50% HCl to immediately preserve any DMSP passing through the GF/F filter, which is defined as dissolved DMSP (DMSPd). Additional time points from each bottle were collected at 3 and 6 h. The rate of change of DMSPd in no-treatment bottles was subtracted from the rate of change in the +GBT bottles to obtain an estimate of DMSPd consumption rate (Kiene and Gerard, 1995).</p>
<p>&lt; 5 µm DMSPd: The DMSP that was less than 5 µm was measured in water from the cubitainer in the lab, using the drip filtration protocol, as described above for DMSPd, except that a 5.0 µm pore size, track-etched polycarbonate filter was used for the filtration.</p>
<p>DMSP Analysis: DMSP was quantified by proxy as the amount of DMS released from samples after alkaline cleavage (White, 1982). For DMSPt, 0.05 to 0.5 ml of each preserved sample was pipetted into a 14 ml glass serum vial, with the volume being adjusted based on the concentration of DMSPt in the sample. For DMSPd, the volume pipetted was 1.0 to 3.0 ml. Each serum vial was treated with 1 ml of 5 M NaOH and capped with a Teflon-faced serum stopper (Wheaton). After 1 h, the amount of DMS in each vial was quantified by purge and trap gas chromatography with flame photometric detection. Briefly, each vial was attached to the purge system and a flow of helium (90-100 ml per minute) allowed bubbling of the solution. An excurrent needle led to a Nafion dryer and six-port valve (Valco). The DMS in the samples was cryotrapped in a Teflon tubing loop immersed in liquid nitrogen. After a 4 min sparge, during which &gt;99% of the DMS in the samples was removed, hot water replaced the liquid nitrogen to introduce the DMS into the Shimadzu GC-2014 gas chromatograph. Separation of the sulfur gases was achieved with a Chromosil 330 column (Supelco; Sigma) maintained at 60 degrees C with a helium carrier flow of 25 ml per minute. The flame photometric detector was operated in sulfur mode and maintained at 175 degrees C. Minimum detection limits during this study were 0.5 to 1 pmol DMS per sample with minimum detectable concentrations ranging from 0.17 to 10 nM, depending on the volume analyzed. The GC-FPD system was calibrated with a gas stream containing known amounts of DMS from a permeation system.</p>
<p>Problem report:&nbsp;For November chlorophyll a<em> </em>samples, fluorescence after acid addition not measured but estimated from samples with similar total fluorescence (Pennington and Chavez, 2000).</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- modified parameter names (removed units; replaced spaces with underscores);<br />
- re-formatted date to yyyy-mm-dd;<br />
- added date/time columns (Local and UTC)&nbsp;in ISO8601 format;<br />
- filled in blank cells with "nd" (no data);<br />
- 2019-11-08: replaced data with version 2.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Beckman Coulter Altra
Beckman Coulter Altra
PI Supplied Instrument Name: Beckman Coulter Altra Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Shimadzu GC-2014 gas chromatograph
Shimadzu GC-2014 gas chromatograph
PI Supplied Instrument Name: Shimadzu GC-2014 gas chromatograph Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
Turner Designs 10-AU Fluorometer
Turner Designs 10-AU Fluorometer
PI Supplied Instrument Name: Turner Designs 10-AU Fluorometer Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
Deployment: Moran_Monterey_2016
Moran_Monterey_2016
Environmental Sample Processor
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Moran_Monterey_2016
Brent Nowinski
University of Georgia
Environmental Sample Processor
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