http://lod.bco-dmo.org/id/dataset/756510
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-02-21
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26C and 30C and reciprocally grown at 26C and 30C, Oct - Dec 2018
2019-02-20
publication
2019-02-20
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-04
publication
https://doi.org/10.1575/1912/bco-dmo.756510.1
Mary Alice Coffroth
State University of New York at Buffalo
principalInvestigator
Casey terHorst
California State University Northridge
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Coffroth, M. A., terHorst, C. (2019) Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26C and 30C and reciprocally grown at 26C and 30C, Oct - Dec 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-02-20 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.756510.1 [access date]
Cell counts for B. antillogorgium cultures Dataset Description: <p>Cell counts of <em>Breviolum </em><em>antillogorgium</em> cultures isolated from the octocoral <em>Antillogorgia bipinnata</em> by sample and treatment for cultures historically grown at 26 and 30 degrees C and reciprocally grown at 26 and 30 degrees C.</p> Methods and Sampling: <p><em>Breviolum antillogorgium</em> cultures were initially isolated from <em>Antillogorgia bipinnata </em>colonies collected at in the upper Florida Reef tract at Elbow Reef (N 25 07.956 W 80 15.810) in September 2016.&nbsp; Culture isolations and cell growth experimentation occurred in the Coffroth lab, University at Buffalo.</p>
<p>A total of six <em>Breviolum antillogorgium </em>genotypes were used in this experiment. Three cultures had been grown at 26 degrees C since isolation in 2016 (16-0590F, 16-0875 and 16-1631) and three had been grown at 30 degrees C since isolation in 2016 (16-0587, 16-0763 and 16-0764). Three replicates of each of the six <em>Breviolum antillogorgium </em>cultures were grown at 26 and 30 C for a total of four treatments (26 degrees at 26 and 30 C and 30 degrees C at 26 and 30 C). A total of four to six replicate counts from each culture was made every three to twelve days using light microscopy and a hemocytometer. Mean symbiont density per ml was calculated.</p>
<p>Data Processing: Mean x 10000 were calculated from the counts.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559286 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559286
completed
Mary Alice Coffroth
State University of New York at Buffalo
716-645-4871
Department of Geology 126 Cooke Hall
Buffalo
NY
14260
USA
Coffroth@buffalo.edu
pointOfContact
Casey terHorst
California State University Northridge
818-677-3352
Department of Biology 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
casey.terhorst@csun.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Day
Culture_ID
Count_1
Count_2
Count_3
Count_4
Count_5
Count_6
Mean
Reichert Brightline hemocytometer
Zeiss
theme
None, User defined
date
days
sample identification
cell_concentration
featureType
BCO-DMO Standard Parameters
Hemocytometer
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
https://osprey.bco-dmo.org/project/632538
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
<p><em>Description from NSF award abstract:</em><br />
On coral reefs, mutualisms with single celled algae (Symbiodinium) and reef species literally and figuratively form the foundation of reef ecosystems. Coral reefs are among the most threatened ecosystems under a changing climate and are rapidly declining due to increasing levels of environmental stress, namely increased temperatures. Climate change is resulting in even warmer ocean temperatures that threaten associations between Symbiodinium and their hosts. In this project the investigators examine the genetic diversity of Symbiodinium and the potential for this important species to evolve in response to temperature. The project will also address whether the ecological and evolutionary dynamics of the Symbiodinium population affect the performance of their host. If so, this suggests that the evolution of microscopic organisms with short generation times could confer adaptation to longer-lived host species on ecologically and economically vital coral reefs. Given that diversity is already being lost on many reefs, considering how evolutionary changes in Symbiodinium will affect reef species is crucial for predicting the responses of reefs to future climate change. This project provides training for two graduate students and several undergraduates at a Hispanic-serving institution. This work includes outreach to the students and the general public through the Aquarium of Niagara, local K-12 schools, and web-based education modules.</p>
<p>The effects of evolution on contemporary ecological processes are at the forefront of research in evolutionary ecology. This project will answer the call for experiments elucidating the effects of genetic variation in Symbiodinium performance and the effect on the response of the holobiont (host and symbiont) to increased temperature. These experiments examine the effects of temperature through both ecological and evolutionary mechanisms and will determine the relative importance of adaptation and acclimatization in replicated experimental populations. The investigators will examine how genetic variation within a species (Symbiodinium antillogorgium) affects symbiont performance in culture and in the host and how this affects the response of the holobiont to increased temperature. Further, the project examines whether holobiont response to increased temperature associated with climate change depends on particular GxG host-symbiont combinations. Moreover, the investigators will examine the effects of symbiont history on mutualist hosts, which have been largely ignored in eco-evolutionary studies. These experiments provide a first step in predicting whether invertebrate hosts on coral reefs will respond to global change via adaptation of their symbionts.</p>
Host Symbiont Temp Response
largerWorkCitation
project
eng; USA
biota
oceans
-80.2635
-80.2635
25.1326
25.1326
2018-10-02
2018-12-24
Florida Keys, Caribbean
0
BCO-DMO catalogue of parameters from Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26C and 30C and reciprocally grown at 26C and 30C, Oct - Dec 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/757473.rdf
Name: Date
Units: unitless
Description: <p>date of count formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/757474.rdf
Name: Day
Units: days
Description: <p>Number of days since inoculation</p>
http://lod.bco-dmo.org/id/dataset-parameter/757475.rdf
Name: Culture_ID
Units: unitless
Description: <p>Identification of sample given as culture name (16-0587; 16-0590F; 16-1631; etc.); treatment temperature (26 or 30 C) and replicate (1; 2; 3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/757476.rdf
Name: Count_1
Units: cells x 10^4/milliliter (cells x 10^4 ml-1)
Description: <p>First replicate of culture counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/757477.rdf
Name: Count_2
Units: cells x 10^4/milliliter (cells x 10^4 ml-1)
Description: <p>Second replicate of culture counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/757478.rdf
Name: Count_3
Units: cells x 10^4/milliliter (cells x 10^4 ml-1)
Description: <p>Third replicate of culture counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/757479.rdf
Name: Count_4
Units: cells x 10^4/milliliter (cells x 10^4 ml-1)
Description: <p>Fourth replicate of culture counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/757480.rdf
Name: Count_5
Units: cells x 10^4/milliliter (cells x 10^4 ml-1)
Description: <p>Fifth replicate of culture counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/757481.rdf
Name: Count_6
Units: cells x 10^4/milliliter (cells x 10^4 ml-1)
Description: <p>Sixth replicate of culture counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/757482.rdf
Name: Mean
Units: cells x 10^4/milliliter (cells x 10^4 ml-1)
Description: <p>Mean of replicate counts</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
31847
https://darchive.mblwhoilibrary.org/bitstream/1912/23755/1/dataset-756510_breviolum-antillogorgium-cell-counts__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.756510.1
download
onLine
dataset
<p><em>Breviolum antillogorgium</em> cultures were initially isolated from <em>Antillogorgia bipinnata </em>colonies collected at in the upper Florida Reef tract at Elbow Reef (N 25 07.956 W 80 15.810) in September 2016.&nbsp; Culture isolations and cell growth experimentation occurred in the Coffroth lab, University at Buffalo.</p>
<p>A total of six <em>Breviolum antillogorgium </em>genotypes were used in this experiment. Three cultures had been grown at 26 degrees C since isolation in 2016 (16-0590F, 16-0875 and 16-1631) and three had been grown at 30 degrees C since isolation in 2016 (16-0587, 16-0763 and 16-0764). Three replicates of each of the six <em>Breviolum antillogorgium </em>cultures were grown at 26 and 30 C for a total of four treatments (26 degrees at 26 and 30 C and 30 degrees C at 26 and 30 C). A total of four to six replicate counts from each culture was made every three to twelve days using light microscopy and a hemocytometer. Mean symbiont density per ml was calculated.</p>
<p>Data Processing: Mean x 10000 were calculated from the counts.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing notes:</strong>- created separate files for each daily cell count<br />
- added columns for Count_5 and Count_6 to data from day 56 to day 83 to match days 1 to 55<br />
- created a toplevel file with date and day from each Excel sheet, which calls the daily files</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Reichert Brightline hemocytometer
Reichert Brightline hemocytometer
PI Supplied Instrument Name: Reichert Brightline hemocytometer PI Supplied Instrument Description:Used to make cell counts. Instrument Name: Hemocytometer Instrument Short Name:Hemocytometer Instrument Description: A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
Zeiss
Zeiss
PI Supplied Instrument Name: Zeiss Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/