<div><p>Push cores (6.35 cm diameter) of sediment were collected in situ using the manipulator arm of the Hybrid Remotely Operated Vehicle Nereus on the margin of the Kermadec Trench (6013m) and along the main trench axis (7137 to 9177m) at roughly 1000m increments.</p>
<p>Cores were sectioned at 1cm intervals and sieved for the meiofaunal fraction.</p>
<p>Upon retrieval of sediment cores, the 0-1, 1-2, 2-3, 3-5, 5-10 cm depth horizons were sectioned and stored frozen (-80 °C) until analysis. Benthic bacterial biomass were calculated from sediment concentrations of the bacterial biomarker phospholipid fatty acids (PLFAs) i14:0, i15:0, ai15:0, i16:0. In brief, PLFAs were extracted and purified from known quantities of freeze-dried sediment samples and subsequently derivatized to yield fatty acid methyl esters (FAMEs). FAMEs were quantified using GC-FID (Agilent Technologies 6890N). Bacterial biomass was calculated using the well-established PLFA:C conversion factor of 0.056 gC PLFA/gC biomass.</p>
<p>Attempts were made to obtain 3 replicate cores at each depth horizon (every 1000 meters from 6000m to 10,000m. For logistical reasons with vehicle performance (and loss at 10,000), all of the replicates were not able to be collected.</p></div>
HADES-K bacterial biomarker phospholipid fatty acid (PLFA)
<div><p>This dataset contains the sample log for HADESK bacterial biomarker phospholipid fatty acid (PLFA) content from sediment push cores. The same samples were also used for the organic matter (OM) analysis. Samples were taken in the Kermadec Trench in the Southwest Pacific, 4000 to ~10,000m from the RV/ Thomas G. Thompson during cruise TN309, May 2014.</p></div>
HADES-K bacterial biomarker phospholipid fatty acid (PLFA)
<div><p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- re-formatted date from m/d/yyyy to yyyy-mm-dd</p>
<p> </p></div>
763873
HADES-K bacterial biomarker phospholipid fatty acid (PLFA)
2019-03-28T09:17:19-04:00
2019-03-28T09:17:19-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:763873
Sample log for HADESK bacterial biomarker phospholipid fatty acid content from sediment cores collected on R/V Thomas G. Thompson cruise TN309, May 2014
This dataset contains the sample log for HADESK bacterial biomarker phospholipid fatty acid (PLFA) content from sediment push cores. The same samples were also used for the organic matter (OM) analysis. Samples were taken in the Kermadec Trench in the Southwest Pacific, 4000 to ~10,000m from the RV/ Thomas G. Thompson during cruise TN309, May 2014.
false
Shank, T., Drazen, J., Yancey, P. (2019) Sample log for HADESK bacterial biomarker phospholipid fatty acid content from sediment cores collected on R/V Thomas G. Thompson cruise TN309, May 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-03-28 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.763873.1 [access date]
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10.1575/1912/bco-dmo.763873.1
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2019-03-28
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