http://lod.bco-dmo.org/id/dataset/766157
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-05-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Enzyme-linked fluorescence (ELF) analysis results from Trichodesmium colony incubations from RV/Atlantis cruise AT39-05, Feb-Mar 2018
2019-05-02
publication
2019-05-02
revision
BCO-DMO Linked Data URI
2019-05-02
creation
http://lod.bco-dmo.org/id/dataset/766157
Eric A. Webb
University of Southern California
principalInvestigator
David A. Hutchins
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Webb, E. A., Hutchins, D. A. (2019) Enzyme-linked fluorescence (ELF) analysis results from Trichodesmium colony incubations from RV/Atlantis cruise AT39-05, Feb-Mar 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2019-05-02 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/766157 [access date]
ELF incubations Dataset Description: <p>These data report enzyme-linked fluorescence (ELF) analysis results from Trichodesmium colony incubations from RV/Atlantis cruise AT39-05, Feb-Mar 2018.</p>
<div>&nbsp;</div> Methods and Sampling: <p>Methods are described in Dyhrman et al (2002).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1657757 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1657757
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1657766 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1657766
onGoing
Eric A. Webb
University of Southern California
213-740-7954
Department of Biological Sciences, Marine and Environmental Biology Section; Dornsife College of Letters, Arts, and Sciences 3616 Trousdale Parkway AHF 331
Los Angeles
CA
90089-0371
USA
eawebb@usc.edu
pointOfContact
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
asNeeded
Unknown
station
lat
lon
slide_label
colony_description
Tricho_filament_thickness
Tricho_filament_fluor
Tricho_filament_ELF_glow
ELF_reaction_results_plus_minus
backgrnd_speckling
backgrnd_speckling_filamaments
backgrnd_speckling_colonies
ZeissAxioplan2 microscope
theme
None, User defined
station
latitude
longitude
sample identification
sample description
featureType
BCO-DMO Standard Parameters
Microscope - Optical
instrument
BCO-DMO Standard Instruments
AT39-05
service
Deployment Activity
Tropical and subtropical Atlantic; 20 N - 8 S, 22 W - 67 W
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean
https://osprey.bco-dmo.org/project/724451
Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean
<p>NSF abstract:</p>
<p>Marine cyanobacteria are able to use or "fix" atmospheric nitrogen gas, and so supply much of the essential nutrient nitrogen that supports open ocean food chains. Oceanographers have usually thought that the growth of these nitrogen-fixing cyanobacteria is limited at any particular time and place by the supply of either iron, or of phosphorus. Preliminary experiments have shown, though, that these nitrogen fixers instead grow best when both iron and phosphorus are scarce at the same time. In this project, the researchers will use cellular indicators that are specific for iron and phosphorus limitation to determine how important this type of "balanced limitation" of nitrogen-fixing cyanobacteria is in controlling the productivity of ocean food chains in the tropical Atlantic Ocean. Two graduate students will be trained at the University of Southern California (USC) and Woods Hole Oceanographic Institution, as well as a postdoctoral researcher at USC. Educational outreach efforts will take place at a Los Angeles inner city high school with a student body that is over 98% Hispanic and African-American, and with underrepresented undergraduates in the USC Global Environmental Microbiology course. In addition, two Research Experiences for Undergraduates students will be supervised for summer research projects to help them learn about science career options.</p>
<p>The researchers will investigate the biological and biogeochemical consequences of this unique balanced iron/phosphorus-limited phenotype, using both laboratory and fieldwork approaches. During the first year of this project, the nitrogen-fixing cyanobacteria will be cultured under iron and/or phosphorus limitation, followed by application of proteomics and transcriptomics to identify genes that are potential diagnostic biomarkers for iron/phosphorus balanced limitation. Preliminary work has already identified one promising candidate biomarker in one cyanobacterium, an EzrA protein domain that appears to be associated with the cell size decreases seen specifically under balanced limitation, and the researchers have identified numerous other potential candidates for similar biomarkers. During the second year, these new co-limitation biomarkers and others previously validated for iron limitation (IsiB) and phosphorus limitation (SphX) will be used to investigate balanced limitation during a research cruise transecting from relatively high-iron, low-phosphorus North Atlantic waters, to the relatively high-phosphorus, low-iron South Atlantic. This fieldwork component will survey nitrogen fixing cyanobacteria populations across this natural iron/phosphorus gradient for genetic, proteomic, and physiological indicators of balanced limitation, as well as testing their responses to iron and phosphorus manipulations in shipboard incubation experiments. The third year will be devoted to sample analysis, and publications exploring the responses of oceanic nitrogen fixers to simultaneous limitation by both iron and phosphorus.</p>
TriCoLim
largerWorkCitation
project
eng; USA
biota
oceans
Tropical and subtropical Atlantic; 20 N - 8 S, 22 W - 67 W
-65.4
-22
-5.6
17
2019-05-02
Tropical Atlantic
0
BCO-DMO catalogue of parameters from Enzyme-linked fluorescence (ELF) analysis results from Trichodesmium colony incubations from RV/Atlantis cruise AT39-05, Feb-Mar 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/766167.rdf
Name: station
Units: unitless
Description: <p>station identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/766168.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/766169.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/766170.rdf
Name: slide_label
Units: unitless
Description: <p>slide label</p>
http://lod.bco-dmo.org/id/dataset-parameter/766171.rdf
Name: colony_description
Units: unitless
Description: <p>Whether Trichodesmium colony morphology is Puff/Tuft/Raft</p>
http://lod.bco-dmo.org/id/dataset-parameter/766172.rdf
Name: Tricho_filament_thickness
Units: unitless
Description: <p>Filament Thickness (e.g. normal; thick; some normal some thick)</p>
http://lod.bco-dmo.org/id/dataset-parameter/766173.rdf
Name: Tricho_filament_fluor
Units: unitless
Description: <p>Trichodesmium filament fluorescence pigmentation on DAPI LP filter (orange; reddish; greenish)</p>
http://lod.bco-dmo.org/id/dataset-parameter/766174.rdf
Name: Tricho_filament_ELF_glow
Units: unitless
Description: <p>Filament ELF Glow (e.g. none/few/some/majority lightly/moderately speckled</p>
http://lod.bco-dmo.org/id/dataset-parameter/766175.rdf
Name: ELF_reaction_results_plus_minus
Units: unitless
Description: <p>?</p>
http://lod.bco-dmo.org/id/dataset-parameter/766176.rdf
Name: backgrnd_speckling
Units: unitless
Description: <p>background speckling (e.g. none; little; some; a lot)</p>
http://lod.bco-dmo.org/id/dataset-parameter/766177.rdf
Name: backgrnd_speckling_filamaments
Units: unitless
Description: <p>speckling around filaments (e.g. none; little; some; a lot)</p>
http://lod.bco-dmo.org/id/dataset-parameter/766178.rdf
Name: backgrnd_speckling_colonies
Units: unitless
Description: <p>speckling around colonies (e.g. none; little; some; a lot - n/a if no colony)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/766157/data/download
download
onLine
dataset
<p>Methods are described in Dyhrman et al (2002).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:<br />
-&nbsp;</strong>added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
ZeissAxioplan2 microscope
ZeissAxioplan2 microscope
PI Supplied Instrument Name: ZeissAxioplan2 microscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
Cruise: AT39-05
AT39-05
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT39-05
David A. Hutchins
University of Southern California
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel