http://lod.bco-dmo.org/id/dataset/766334
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-05-06
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Nitrogen and carbon fixation rates from bulk water samples from RV/Atlantic cruise AT39-05, Feb-Mar 2018
2019-05-02
publication
2019-05-02
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-05-04
publication
https://doi.org/10.26008/1912/bco-dmo.766334.1
David A. Hutchins
University of Southern California
principalInvestigator
Eric A. Webb
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hutchins, D., Webb, E. (2020) Nitrogen and carbon fixation rates from bulk water samples from RV/Atlantic cruise AT39-05, Feb-Mar 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-05-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.766334.1 [access date]
Nitrogen and carbon fixation rates - bulk seawater Dataset Description: <p>These data reports nitrogen and carbon fixation rates from bulk water samples collected on RV/Atlantic cruise AT39-05, Feb-Mar 2018.</p> Methods and Sampling: <p>Shipboard incubation experiments were carried out at 15 stations in the tropical Atlantic with trace metal clean-collected bulk water to examine iron (Fe) and phosphorus (P) co-limitation of dinitrogen (N2) fixation (see TriCoLim Station coordinates metadata). Treatments were: Co-limited control (no additions, “Colimit”), + 2nM Fe (“Fe”), + 50 nM P (“P”) and Replete (+Fe+P, “Rep”).&nbsp;All incubations were carried out at ambient (“Lo”) temperature and ambient temperature +3C (“Hi”) for 2-4 days on deck before measuring 15N2 and 13CO2 fixation rates and particulate organic carbon (POC) and nitrogen (PON). See related datasets.</p>
<table style="line-height:inherit; width:705px">
<tbody>
<tr>
<td><a href="https://www.bco-dmo.org/dataset/766354">Nitrogen and carbon fixation rates - Trichodesmium</a></td>
<td>Nitrogen and carbon fixation rates from Trichodesmium colonies from RV/Atlantic cruise AT39-05, Feb-Mar 2018</td>
</tr>
<tr>
<td><a href="https://www.bco-dmo.org/dataset/766374">POC and PON - bulk seawater</a></td>
<td>POC and PON from bulk seawater samples from RV/Atlantic cruise AT39-05, Feb-Mar 2018</td>
</tr>
<tr>
<td><a href="https://www.bco-dmo.org/dataset/766394">POC and PON - Trichodesmium</a></td>
<td>POC and PON from Trichodesmium colonies from RV/Atlantic cruise AT39-05, Feb-Mar 2018</td>
</tr>
</tbody>
</table>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1657757 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1657757
completed
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
Eric A. Webb
University of Southern California
213-740-7954
Department of Biological Sciences, Marine and Environmental Biology Section; Dornsife College of Letters, Arts, and Sciences 3616 Trousdale Parkway AHF 331
Los Angeles
CA
90089-0371
USA
eawebb@usc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
Experiment_ID
Latitude
Longitude
Sample_D
Treatment
N2_fix
C_fix
theme
None, User defined
station
experiment id
latitude
longitude
sample identification
treatment
nitrogen fixation rate
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Trace Metal Bottle
instrument
BCO-DMO Standard Instruments
AT39-05
service
Deployment Activity
Tropical and subtropical Atlantic; 20 N - 8 S, 22 W - 67 W
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean
https://osprey.bco-dmo.org/project/724451
Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean
<p>NSF abstract:</p>
<p>Marine cyanobacteria are able to use or "fix" atmospheric nitrogen gas, and so supply much of the essential nutrient nitrogen that supports open ocean food chains. Oceanographers have usually thought that the growth of these nitrogen-fixing cyanobacteria is limited at any particular time and place by the supply of either iron, or of phosphorus. Preliminary experiments have shown, though, that these nitrogen fixers instead grow best when both iron and phosphorus are scarce at the same time. In this project, the researchers will use cellular indicators that are specific for iron and phosphorus limitation to determine how important this type of "balanced limitation" of nitrogen-fixing cyanobacteria is in controlling the productivity of ocean food chains in the tropical Atlantic Ocean. Two graduate students will be trained at the University of Southern California (USC) and Woods Hole Oceanographic Institution, as well as a postdoctoral researcher at USC. Educational outreach efforts will take place at a Los Angeles inner city high school with a student body that is over 98% Hispanic and African-American, and with underrepresented undergraduates in the USC Global Environmental Microbiology course. In addition, two Research Experiences for Undergraduates students will be supervised for summer research projects to help them learn about science career options.</p>
<p>The researchers will investigate the biological and biogeochemical consequences of this unique balanced iron/phosphorus-limited phenotype, using both laboratory and fieldwork approaches. During the first year of this project, the nitrogen-fixing cyanobacteria will be cultured under iron and/or phosphorus limitation, followed by application of proteomics and transcriptomics to identify genes that are potential diagnostic biomarkers for iron/phosphorus balanced limitation. Preliminary work has already identified one promising candidate biomarker in one cyanobacterium, an EzrA protein domain that appears to be associated with the cell size decreases seen specifically under balanced limitation, and the researchers have identified numerous other potential candidates for similar biomarkers. During the second year, these new co-limitation biomarkers and others previously validated for iron limitation (IsiB) and phosphorus limitation (SphX) will be used to investigate balanced limitation during a research cruise transecting from relatively high-iron, low-phosphorus North Atlantic waters, to the relatively high-phosphorus, low-iron South Atlantic. This fieldwork component will survey nitrogen fixing cyanobacteria populations across this natural iron/phosphorus gradient for genetic, proteomic, and physiological indicators of balanced limitation, as well as testing their responses to iron and phosphorus manipulations in shipboard incubation experiments. The third year will be devoted to sample analysis, and publications exploring the responses of oceanic nitrogen fixers to simultaneous limitation by both iron and phosphorus.</p>
TriCoLim
largerWorkCitation
project
eng; USA
oceans
Tropical and subtropical Atlantic; 20 N - 8 S, 22 W - 67 W
-55.8123
-21.998
-7.9989
13.6776
2018-02-11
2018-03-14
Tropical Atlantic
0
BCO-DMO catalogue of parameters from Nitrogen and carbon fixation rates from bulk water samples from RV/Atlantic cruise AT39-05, Feb-Mar 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/766346.rdf
Name: Station
Units: unitless
Description: <p>station identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/766347.rdf
Name: Experiment_ID
Units: unitless
Description: <p>experiment_id identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/766348.rdf
Name: Latitude
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/766349.rdf
Name: Longitude
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/766350.rdf
Name: Sample_D
Units: unitless
Description: <p>sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/766351.rdf
Name: Treatment
Units: unitless
Description: <p>treatment:<br />
Lo temp = ambient temperature<br />
Hi temp = ambient temperature plus 3 degrees C.<br />
Colimit = Co-limited control (no additions)<br />
Fe = 2nM Fe<br />
P = 50 nM P<br />
Rep = Replete (+Fe+P)</p>
http://lod.bco-dmo.org/id/dataset-parameter/766352.rdf
Name: N2_fix
Units: micromol/liter/hour (umol/l/h)
Description: <p>nitrogen fixation rate measured as 15N2</p>
http://lod.bco-dmo.org/id/dataset-parameter/766353.rdf
Name: C_fix
Units: micromol/liter/hour (umol/l/h)
Description: <p>carbon fixation rate measured as 13CO2</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
16525
https://darchive.mblwhoilibrary.org/bitstream/1912/25746/1/dataset-766334_nitrogen-and-carbon-fixation-rates-bulk-seawater__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.766334.1
download
onLine
dataset
<p>Shipboard incubation experiments were carried out at 15 stations in the tropical Atlantic with trace metal clean-collected bulk water to examine iron (Fe) and phosphorus (P) co-limitation of dinitrogen (N2) fixation (see TriCoLim Station coordinates metadata). Treatments were: Co-limited control (no additions, “Colimit”), + 2nM Fe (“Fe”), + 50 nM P (“P”) and Replete (+Fe+P, “Rep”).&nbsp;All incubations were carried out at ambient (“Lo”) temperature and ambient temperature +3C (“Hi”) for 2-4 days on deck before measuring 15N2 and 13CO2 fixation rates and particulate organic carbon (POC) and nitrogen (PON). See related datasets.</p>
<table style="line-height:inherit; width:705px">
<tbody>
<tr>
<td><a href="https://www.bco-dmo.org/dataset/766354">Nitrogen and carbon fixation rates - Trichodesmium</a></td>
<td>Nitrogen and carbon fixation rates from Trichodesmium colonies from RV/Atlantic cruise AT39-05, Feb-Mar 2018</td>
</tr>
<tr>
<td><a href="https://www.bco-dmo.org/dataset/766374">POC and PON - bulk seawater</a></td>
<td>POC and PON from bulk seawater samples from RV/Atlantic cruise AT39-05, Feb-Mar 2018</td>
</tr>
<tr>
<td><a href="https://www.bco-dmo.org/dataset/766394">POC and PON - Trichodesmium</a></td>
<td>POC and PON from Trichodesmium colonies from RV/Atlantic cruise AT39-05, Feb-Mar 2018</td>
</tr>
</tbody>
</table>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:<br />
-&nbsp;</strong>added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
-&nbsp;reduced lat and lon precision from 14 to 3 places to better reflect sampling precision</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Used to collect clean seawater. Instrument Name: Trace Metal Bottle Instrument Short Name:TM Bottle Instrument Description: Trace metal (TM) clean rosette bottle used for collecting trace metal clean seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/
Cruise: AT39-05
AT39-05
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT39-05
David A. Hutchins
University of Southern California
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel