http://lod.bco-dmo.org/id/dataset/767141
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-05-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Limits of detection and qPCR efficiencies from cruise SAV 17-16 in the South Atlantic Bight aboard the R/V Savannah from 2011 to 2017
2019-05-08
publication
2019-05-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-21
publication
https://doi.org/10.1575/1912/bco-dmo.767141.1
James T. Hollibaugh
University of Georgia
principalInvestigator
Brian N. Popp
University of Hawai'i
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hollibaugh, J., Popp, B. (2019) Limits of detection and qPCR efficiencies from cruise SAV 17-16 in the South Atlantic Bight aboard the R/V Savannah from 2011 to 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-05-08 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.767141.1 [access date]
Limits of detection and qPCR efficiencies from cruise SAV 17-16 in the South Atlantic Bight aboard the R/V Savannah from 2011 to 2017 Dataset Description: <p>Samples were collected from four regions (inshore, midshelf, shelf-break, and oceanic) of the SAB off the Georgia (U.S.A.) coast (Fig. 1; Supporting Information Table S1), with terminology modified from Liu et al. (2018) as follows. “Inshore” stations were within the barrier island complex. “Mid-shelf” stations were outside the barrier island complex to depths &lt; 40 m; due to limited sampling in this zone, no demarcation between “mid-shelf” and “nearshore” stations (as in Liu et al. 2018) was made. “Shelf-break” stations were between 40 m and 500 m depth. While Liu et al. (2018) did not sample waters past the shelf-break, we included deeper stations further offshore (bottom depth &gt; 500 m), which are designated “oceanic” stations. Note that the maximum depth sampled was ≤ 500 m due to equipment limitations.&nbsp;</p>
<p>Inshore samples were collected from a dock at Marsh Landing on the Duplin River (Sapelo Island) and the dock at the Skidaway Institute of Oceanography (Fig. 1). Both inshore sites are salt marsh-dominated estuaries. Water from both sites was sampled from a depth ≤ 1 m and was processed immediately at a nearby laboratory (the University of Georgia Marine Institute on Sapelo Island or onboard the R/V Savannah). Water quality data for Marsh Landing samples were collected as part of the Sapelo Island National Estuarine Research Reserve monitoring program. Relevant data from the Lower Duplin (“LD”) sonde were downloaded from NOAA/CDMO (http://cdmo. baruch.sc.edu/aqs/, last accessed 22 May 2018).&nbsp;</p>
<p>Most SAB samples were collected in August 2017 on the R/V Savannah (cruise SAV-17-16) along transects across the continental shelf and the Gulf Stream and into the western Sargasso Sea, with sampling focused around the shelf-break (Fig. 1). Water from multiple depths was collected using 12-liter Niskin bottles mounted on a rosette equipped with a Sea-Bird SBE25 CTD. Profiles of salinity, temperature, dissolved oxygen, fluorescence, and photosynthetically active radiation (PAR) were collected using the CTD system as described previously (Liu et al. 2018). PAR attenuation (Kd) was calculated from plots of ln(PAR) vs. depth as in Liu et al. (2018).&nbsp; Two additional SAB stations were sampled in October 2011 (described previously by Liu et al. 2015 and Tolar et al. 2017) and are referred to as “2011-4” and “2011-12” (Fig. 1). Environmental data and some of the microbial and rate data from 2011 stations are available in other publications (Liu et al. 2015; Tolar et al. 2017; see BCO-DMO dataset DON_Oxidation <a href="https://www.bco-dmo.org/dataset/767048">https://www.bco-dmo.org/dataset/767048</a>).&nbsp;</p> Methods and Sampling: <p><strong>Nutrient analysis</strong></p>
<p>Nutrient samples were filtered through 0.22&nbsp;<strong>μ</strong>m pore size Durapore&nbsp;GVWP filters (Millipore Sigma) and frozen at&nbsp;<strong>−</strong>20<strong>_</strong>C immediately&nbsp;after collection, then stored at&nbsp;<strong>−</strong>80<strong>_</strong>C until analysis.&nbsp;Dissolved nitrate (NO3&nbsp;<strong>−</strong>), nitrite (NO2&nbsp;<strong>−</strong>), phosphate (PO4&nbsp;3<strong>−</strong>), and&nbsp;silicate (SiO4&nbsp;4<strong>−</strong>) were measured using a Bran and Luebbe AA3&nbsp;autoanalyzer as described previously (Wilkerson et al. 2015).&nbsp;Ammonium and urea were measured manually using the&nbsp;phenolhypochlorite method (Solórzano 1969) and the diacetylmonoxime&nbsp;method (Rahmatullah and Boyde 1980; Mulvenna&nbsp;and Savidge 1992), respectively.&nbsp;</p>
<p><strong>Oxidation rate measurements&nbsp;</strong></p>
<p>We used 15N-labeled substrates (98<strong>–</strong>99% 15N, Cambridge&nbsp;Isotope Laboratories) to measure the oxidation of N supplied&nbsp;as NH4+, urea, 1,2-diaminoethane (DAE), 1,3-diaminopropane&nbsp;(DAP), 1,4-diaminobutane (putrescine, PUT), L-glutamic acid&nbsp;(GLU), and L-arginine (ARG). 15N oxidation from NH4+, urea,&nbsp;PUT, and GLU were measured extensively, whereas 15N oxidation&nbsp;from DAE, DAP, and ARG was only measured at a subset&nbsp;of stations (Supporting Information Table S1). GLU and ARG&nbsp;were included as a control for remineralization, as their central&nbsp;roles in microbial metabolism leads to rapid catabolism and&nbsp;NH4&nbsp;+ regeneration (Hollibaugh 1978; Goldman et al. 1987).&nbsp;PUT was used in routine assessments of the oxidation of&nbsp;polyamine-N because it is one of the most consistently detected&nbsp;polyamines in seawater (Nishibori et al. 2001a, 2003; Lu&nbsp;et al. 2014; Liu et al. 2015). Although spermine and spermidine&nbsp;are also common in seawater, 15N-labeled stocks of these polyamines&nbsp;were not commercially available. We measured the oxidation&nbsp;of N from DAE and DAP to investigate the effect of&nbsp;aliphatic chain length (which affects pK<sub>a</sub>) on oxidation rate.&nbsp;</p>
<p>Duplicate seawater samples contained in 1-liter polycarbonate&nbsp;or 250 mL high density polyethylene (HDPE) bottles&nbsp;wrapped with aluminum foil (to exclude light) were&nbsp;amended with 10<strong>–</strong>50 nM 15N-labeled substrate. Marsh&nbsp;Landing samples were then placed in an incubator held at&nbsp;in situ temperature in the dark. Samples taken at the Skidaway&nbsp;dock were placed in a mesh bag and immersed at the&nbsp;sea surface at the sampling site. Samples collected at sea&nbsp;were incubated in a tank of flowing surface seawater or in an&nbsp;incubator held at 18<strong>&nbsp;</strong>C in the dark. Incubation bottles were sampled&nbsp;for 15N analysis immediately after substrate addition and&nbsp;again after a period of ~ 24 h. 15N samples were subsampled into&nbsp;50 mL polypropylene centrifuge tubes, frozen at&nbsp;<strong>−</strong>20<strong>_</strong>C, and&nbsp;stored at&nbsp;<strong>−</strong>80<strong>_</strong>C until analysis. The 15N/14N ratios of the NO3&nbsp;<strong>−&nbsp;</strong>plus NO2&nbsp;<strong>−&nbsp;</strong>(NOX) pools (<strong>δ</strong>15NNOx) in the samples were measured&nbsp;using the bacterial denitrifier method to convert NOX to nitrous&nbsp;oxide (N2O; Sigman et al. 2001). The&nbsp;<strong>δ</strong>15N values of the N2O&nbsp;produced were measured using a Finnigan MAT-252 isotope&nbsp;ratio mass spectrometer coupled with a modified GasBench II interface (Casciotti et al. 2002; Beman et al. 2011; McIlvin&nbsp;and Casciotti 2011). Oxidation rates were calculated using an&nbsp;endpoint model (Beman et al. 2011; Damashek et al. 2016).&nbsp;Since the substrates used were uniformly labeled with 15N, the&nbsp;amount of the N added as the 15N spike (in&nbsp;<strong>μ</strong>M) was multiplied&nbsp;by the number of moles of 15N per mole of substrate, which&nbsp;assumes that all of the N atoms have equal probability of being&nbsp;oxidized. This is likely true for urea, DAE, DAP, and PUT, which&nbsp;are symmetrical molecules, but not likely to be true for ARG,&nbsp;which contains 4 N atoms (one in the&nbsp;<strong>α</strong>-amino position and&nbsp;three in the guanidine structure of its R-group). Abiotic oxidation&nbsp;of organic N was assessed by measuring 15NOX production&nbsp;following 15N amendment and incubation of 0.22&nbsp;<strong>μ</strong>m filtered&nbsp;seawater (as described above), and potential metabolism of&nbsp;DON by the denitrifying bacteria used to convert NOX to N2O&nbsp;was checked by adding 15N-labeled substrates into the bacterial&nbsp;cultures prior to mass spectrometry.&nbsp;</p>
<p>We were unable to measure the in situ concentrations of&nbsp;the individual components of DON used in oxidation experiments,&nbsp;other than urea. Based on previous measurements&nbsp;made in the SAB (Lu et al. 2014; Liu et al. 2015), we assumed&nbsp;concentrations of 1 nM and 0.25 nM for DAE, DAP and PUT,&nbsp;and 10 nM and 5 nM for GLU and ARG, at inshore and&nbsp;mid-shelf/shelf-break/oceanic stations, respectively. Rates of&nbsp;polyamine and amino acid oxidation reported below should&nbsp;therefore be considered potential rates, as amendments as low&nbsp;as 10<strong>–</strong>50 nM are likely to increase substrate concentrations&nbsp;substantially above in situ. Initial substrate 15N activity was&nbsp;calculated using isotope mass balance using the known concentration&nbsp;and 15N activity of the labeled substrates added&nbsp;and assuming the concentrations described above and natural&nbsp;abundance 15N activity (i.e., 0.3663 atom% 15N).&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1537995 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1537995
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538677 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538677
completed
James T. Hollibaugh
University of Georgia
706-542-7671
Department of Marine Sciences, University of Georgia
Athens
GA
30602
USA
aquadoc@uga.edu
pointOfContact
Brian N. Popp
University of Hawai'i
808-956-6206
Department of Earth Sciences 1680 East-West Road
Honolulu
HI
96822
USA
popp@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Probe
Forward_primer
Reverse_primer
Cycling_conditions
Efficiency
Limit_of_Detection_template
Limit_of_Detection_sample
Number_plates_run
Reference
qPCR_Parameters
Bran and Luebbe AA3 autoanalyzer
Finnigan MAT-252 isotope ratio mass spectrometer
C1000 Touch Thermal Cycler
theme
None, User defined
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Bran Luebbe AA3 AutoAnalyzer
Isotope-ratio Mass Spectrometer
qPCR Thermal Cycler
instrument
BCO-DMO Standard Instruments
SAV-17-16
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Direct Oxidation of Organic Nitrogen by Marine Ammonia Oxidizing Organisms
https://osprey.bco-dmo.org/project/757587
Collaborative Research: Direct Oxidation of Organic Nitrogen by Marine Ammonia Oxidizing Organisms
<p>NSF Abstract:</p>
<p>Nitrogen is an essential nutrient for phytoplankton that often limits primary production in the ocean, and its availability therefore plays a key role in global ocean productivity. The amounts and form in which nitrogen exist are controlled by microorganisms. One microorganism-mediated process is known as nitrification, which oxidizes ammonia or ammonium to nitrite and nitrite to nitrate, nitrate being the bioavailable form of nitrogen. While this is the well-accepted process of nitrification, preliminary results strongly suggest that a nitrogen-containing compound know as polyamine nitrogen may be directly converted by some microorganisms to nitrate. However, the importance of this process for global biogeochemical nitrogen cycling is unknown. The goal of this study is to evaluate the biogeochemical significance of direct oxidation of polyamine nitrogen, as a model organic nitrogen compound, to nitrification compared to canonical nitrification of ammonia. The project will result in training a postdoctoral researcher and provide opportunities for undergraduates to gain hands-on experience with research on microbial geochemistry and coastal ecosystem processes. Project personnel will also work with the Georgia Coastal Ecosystems Long-Term Ecological Research program to engage a K-12 science teacher in the project.</p>
<p>Ammonia oxidation is a key step in the process of converting fixed nitrogen to dinitrogen gas and thus is central to the global nitrogen cycle and to removing excess fixed nitrogen from coastal waters with high concentrations of nutrients. Recent research has shown that Thaumarchaeota play a major role in ammonia oxidation in the ocean. Experiments with enrichment cultures and coastal water samples where ammonia oxidizing archaea are the dominant ammonia oxidizers, show that some forms of organic nitrogen may be oxidized directly to nitrogen oxides without first being regenerated as ammonium. Of the substrates tested, polyamine and particularly putrescine nitrogen appear to be oxidized directly to nitrogen oxides, while amino acid and urea nitrogen is first regenerated as ammonium and then oxidized. The investigators will examine this process in detail over three years using enrichment cultures and experiments conducted with coastal bacterioplankton. Specifically, they will aim to better understand 1) the consequences of this novel process to ocean geochemistry, 2) the fate of the carbon present in polyamines, 3) what organisms are responsible for the direct oxidation, and 4) the chemical characteristics of the organic nitrogen compounds accessible to direct oxidation.</p>
DON Oxidation
largerWorkCitation
project
eng; USA
oceans
-81.356
-78.765
30.3175
31.99
2011-10-04
2017-08-19
Coastal waters and the South Atlantic Bight continental shelf from Savannah GA out to the shelf break (SAV 17-16, UNOLS STR _104733, Marsden Grid 117, Navy Ops NA06), coastal waters around Sapelo Island, Georgia USA
0
BCO-DMO catalogue of parameters from Limits of detection and qPCR efficiencies from cruise SAV 17-16 in the South Atlantic Bight aboard the R/V Savannah from 2011 to 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/767182.rdf
Name: Probe
Units: unitless
Description: <p>Probe. All probes contained a 5' FAM tag and a 3' BHQ1 quencher.</p>
http://lod.bco-dmo.org/id/dataset-parameter/767183.rdf
Name: Forward_primer
Units: unitless
Description: <p>forward primer</p>
http://lod.bco-dmo.org/id/dataset-parameter/767184.rdf
Name: Reverse_primer
Units: unitless
Description: <p>reverse primer</p>
http://lod.bco-dmo.org/id/dataset-parameter/767185.rdf
Name: Cycling_conditions
Units: unitless
Description: <p>Cycling conditions</p>
http://lod.bco-dmo.org/id/dataset-parameter/767186.rdf
Name: Efficiency
Units: unitless
Description: <p>efficiency (%)</p>
http://lod.bco-dmo.org/id/dataset-parameter/767187.rdf
Name: Limit_of_Detection_template
Units: copies per microliter of template
Description: <p>Limit of detection of template</p>
http://lod.bco-dmo.org/id/dataset-parameter/767188.rdf
Name: Limit_of_Detection_sample
Units: copies per liter of sample
Description: <p>Limit of detection of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/767189.rdf
Name: Number_plates_run
Units: count
Description: <p>number of plates run</p>
http://lod.bco-dmo.org/id/dataset-parameter/767190.rdf
Name: Reference
Units: unitless
Description: <p>citation for values</p>
http://lod.bco-dmo.org/id/dataset-parameter/767191.rdf
Name: qPCR_Parameters
Units: unitless
Description: <p>qPCR parameters</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1277
https://darchive.mblwhoilibrary.org/bitstream/1912/24285/1/dataset-767141_qpcrparameters__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.767141.1
download
onLine
dataset
<p><strong>Nutrient analysis</strong></p>
<p>Nutrient samples were filtered through 0.22&nbsp;<strong>μ</strong>m pore size Durapore&nbsp;GVWP filters (Millipore Sigma) and frozen at&nbsp;<strong>−</strong>20<strong>_</strong>C immediately&nbsp;after collection, then stored at&nbsp;<strong>−</strong>80<strong>_</strong>C until analysis.&nbsp;Dissolved nitrate (NO3&nbsp;<strong>−</strong>), nitrite (NO2&nbsp;<strong>−</strong>), phosphate (PO4&nbsp;3<strong>−</strong>), and&nbsp;silicate (SiO4&nbsp;4<strong>−</strong>) were measured using a Bran and Luebbe AA3&nbsp;autoanalyzer as described previously (Wilkerson et al. 2015).&nbsp;Ammonium and urea were measured manually using the&nbsp;phenolhypochlorite method (Solórzano 1969) and the diacetylmonoxime&nbsp;method (Rahmatullah and Boyde 1980; Mulvenna&nbsp;and Savidge 1992), respectively.&nbsp;</p>
<p><strong>Oxidation rate measurements&nbsp;</strong></p>
<p>We used 15N-labeled substrates (98<strong>–</strong>99% 15N, Cambridge&nbsp;Isotope Laboratories) to measure the oxidation of N supplied&nbsp;as NH4+, urea, 1,2-diaminoethane (DAE), 1,3-diaminopropane&nbsp;(DAP), 1,4-diaminobutane (putrescine, PUT), L-glutamic acid&nbsp;(GLU), and L-arginine (ARG). 15N oxidation from NH4+, urea,&nbsp;PUT, and GLU were measured extensively, whereas 15N oxidation&nbsp;from DAE, DAP, and ARG was only measured at a subset&nbsp;of stations (Supporting Information Table S1). GLU and ARG&nbsp;were included as a control for remineralization, as their central&nbsp;roles in microbial metabolism leads to rapid catabolism and&nbsp;NH4&nbsp;+ regeneration (Hollibaugh 1978; Goldman et al. 1987).&nbsp;PUT was used in routine assessments of the oxidation of&nbsp;polyamine-N because it is one of the most consistently detected&nbsp;polyamines in seawater (Nishibori et al. 2001a, 2003; Lu&nbsp;et al. 2014; Liu et al. 2015). Although spermine and spermidine&nbsp;are also common in seawater, 15N-labeled stocks of these polyamines&nbsp;were not commercially available. We measured the oxidation&nbsp;of N from DAE and DAP to investigate the effect of&nbsp;aliphatic chain length (which affects pK<sub>a</sub>) on oxidation rate.&nbsp;</p>
<p>Duplicate seawater samples contained in 1-liter polycarbonate&nbsp;or 250 mL high density polyethylene (HDPE) bottles&nbsp;wrapped with aluminum foil (to exclude light) were&nbsp;amended with 10<strong>–</strong>50 nM 15N-labeled substrate. Marsh&nbsp;Landing samples were then placed in an incubator held at&nbsp;in situ temperature in the dark. Samples taken at the Skidaway&nbsp;dock were placed in a mesh bag and immersed at the&nbsp;sea surface at the sampling site. Samples collected at sea&nbsp;were incubated in a tank of flowing surface seawater or in an&nbsp;incubator held at 18<strong>&nbsp;</strong>C in the dark. Incubation bottles were sampled&nbsp;for 15N analysis immediately after substrate addition and&nbsp;again after a period of ~ 24 h. 15N samples were subsampled into&nbsp;50 mL polypropylene centrifuge tubes, frozen at&nbsp;<strong>−</strong>20<strong>_</strong>C, and&nbsp;stored at&nbsp;<strong>−</strong>80<strong>_</strong>C until analysis. The 15N/14N ratios of the NO3&nbsp;<strong>−&nbsp;</strong>plus NO2&nbsp;<strong>−&nbsp;</strong>(NOX) pools (<strong>δ</strong>15NNOx) in the samples were measured&nbsp;using the bacterial denitrifier method to convert NOX to nitrous&nbsp;oxide (N2O; Sigman et al. 2001). The&nbsp;<strong>δ</strong>15N values of the N2O&nbsp;produced were measured using a Finnigan MAT-252 isotope&nbsp;ratio mass spectrometer coupled with a modified GasBench II interface (Casciotti et al. 2002; Beman et al. 2011; McIlvin&nbsp;and Casciotti 2011). Oxidation rates were calculated using an&nbsp;endpoint model (Beman et al. 2011; Damashek et al. 2016).&nbsp;Since the substrates used were uniformly labeled with 15N, the&nbsp;amount of the N added as the 15N spike (in&nbsp;<strong>μ</strong>M) was multiplied&nbsp;by the number of moles of 15N per mole of substrate, which&nbsp;assumes that all of the N atoms have equal probability of being&nbsp;oxidized. This is likely true for urea, DAE, DAP, and PUT, which&nbsp;are symmetrical molecules, but not likely to be true for ARG,&nbsp;which contains 4 N atoms (one in the&nbsp;<strong>α</strong>-amino position and&nbsp;three in the guanidine structure of its R-group). Abiotic oxidation&nbsp;of organic N was assessed by measuring 15NOX production&nbsp;following 15N amendment and incubation of 0.22&nbsp;<strong>μ</strong>m filtered&nbsp;seawater (as described above), and potential metabolism of&nbsp;DON by the denitrifying bacteria used to convert NOX to N2O&nbsp;was checked by adding 15N-labeled substrates into the bacterial&nbsp;cultures prior to mass spectrometry.&nbsp;</p>
<p>We were unable to measure the in situ concentrations of&nbsp;the individual components of DON used in oxidation experiments,&nbsp;other than urea. Based on previous measurements&nbsp;made in the SAB (Lu et al. 2014; Liu et al. 2015), we assumed&nbsp;concentrations of 1 nM and 0.25 nM for DAE, DAP and PUT,&nbsp;and 10 nM and 5 nM for GLU and ARG, at inshore and&nbsp;mid-shelf/shelf-break/oceanic stations, respectively. Rates of&nbsp;polyamine and amino acid oxidation reported below should&nbsp;therefore be considered potential rates, as amendments as low&nbsp;as 10<strong>–</strong>50 nM are likely to increase substrate concentrations&nbsp;substantially above in situ. Initial substrate 15N activity was&nbsp;calculated using isotope mass balance using the known concentration&nbsp;and 15N activity of the labeled substrates added&nbsp;and assuming the concentrations described above and natural&nbsp;abundance 15N activity (i.e., 0.3663 atom% 15N).&nbsp;</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing Notes:<br />
-&nbsp;added conventional header with dataset name, PI name, version date<br />
-&nbsp;modified parameter names to conform with BCO-DMO naming conventions<br />
- tansposed the table<br />
- combined continuous cells</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Bran and Luebbe AA3 autoanalyzer
Bran and Luebbe AA3 autoanalyzer
PI Supplied Instrument Name: Bran and Luebbe AA3 autoanalyzer PI Supplied Instrument Description:Dissolved nitrate (NO3 −), nitrite (NO2 −), phosphate (PO4 3−), and silicate (SiO4 4−) were measured using a Bran and Luebbe AA3 autoanalyzer as described previously (Wilkerson et al. 2015). Instrument Name: Bran Luebbe AA3 AutoAnalyzer Instrument Short Name:Bran Luebbe AA3 AutoAnalyzer Instrument Description: Bran Luebbe AA3 AutoAnalyzer
See the description from the manufacturer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Finnigan MAT-252 isotope ratio mass spectrometer
Finnigan MAT-252 isotope ratio mass spectrometer
PI Supplied Instrument Name: Finnigan MAT-252 isotope ratio mass spectrometer PI Supplied Instrument Description:The δ15N values of the N2O produced were measured using a Finnigan MAT-252 isotope ratio mass spectrometer coupled with a modified GasBench II interface Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
C1000 Touch Thermal Cycler
C1000 Touch Thermal Cycler
PI Supplied Instrument Name: C1000 Touch Thermal Cycler PI Supplied Instrument Description:All reactions (25 μL total volume) were run in triplicate on a C1000 Touch Thermal Cycler equipped with a CFX96 Real-Time System (Bio- Rad), using either the iTaq Universal Green SYBR Mix (Bio-Rad) or the Platinum qPCR SuperMix-UDG (Thermo Fisher). Instrument Name: qPCR Thermal Cycler Instrument Short Name:qPCR Instrument Description: An instrument for quantitative polymerase chain reaction (qPCR), also known as real-time polymerase chain reaction (Real-Time PCR).
Cruise: SAV-17-16
SAV-17-16
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-16
James T. Hollibaugh
University of Georgia
R/V Savannah
Community Standard Description
R/V Savannah
vessel