http://lod.bco-dmo.org/id/dataset/767285
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-05-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Concentrations of dissolved organic carbon and phenols from Polarstern cruise PS 94-ARK-XXIX/3 from August to October 2015
2019-05-10
publication
2019-05-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-04
publication
https://doi.org/10.1575/1912/bco-dmo.767285.1
Karl Kaiser
Texas A&M, Galveston
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kaiser, K. (2019) Concentrations of dissolved organic carbon and phenols from for Polarstern cruise PS 94-ARK-XXIX/3 from August to October 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-05-10 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.767285.1 [access date]
Concentrations of dissolved organic carbon and phenols from for Polarstern cruise PS 94-ARK-XXIX/3 Dataset Description: Methods and Sampling: <p>Samples were filtered straight from Niskin bottles following established protocols for trace-metal clean sampling (Sample handling protocols for GEOTRACES cruises).</p>
<p>Samples for DOC concentration were acidified to pH 2.5 and analyzed by high-temperature combustion on a Shimadzu TOC-L. DOC was calculated as the mean of between three and five injections using a six-point standard curve.</p>
<p>Lignin-derived phenols were determined following Yan and Kaiser (2018; Anal. Chem), and Yan and Kaiser (2018; Analytica Chimica Acta). Briefly, C18 extracts were redissolved in 200 μL of 1.1 mol L−1 argon- sparged (10 min) NaOH in a 400 uL Teflon vial (Savillex Corp) and amended with containing 500 mg CuO, and amended with 10μL of10 mmolL−1 CuSO4 and 10μ L of 0.2 molL−1 ascorbic acid. Oxidation was at 150 C for 120 minutes. Following oxidation, the samples were spiked with with a surrogate standard mixture of p- hydroxybenzoic acid-13C7, vanillin-13C6, and syringaldehyde-13C6 and acidified to pH ≈ 2.5 with 6 mol L−1 sulfuric acid in the reaction vials.&nbsp; Clean-up of samples was performed with Waters HLB cartrides and final sample eluates were dried under ultra-high purity argon. Phenols were quantified by liquid chromatography/electrospray ionization-tandem mass spectrometry using a five-point calibration curve bracketing the concentration range. Quantified phenols (TDLP included vanillin, acetovanillone, vanillic acid, syringaldehyde, acetosyringone, syringic acid, coumaric acid, ferulic acid, p-hydroxy-benzaldehyde, p-hydroxy-acetophenone, and p-hydroxy-benzoic acid.</p>
<p>Samples were filtered straight from Niskin bottles following established protocols for trace-metal clean sampling (Sample handling protocols for GEOTRACES cruises)</p>
<p>Samples for DOC concentration were acidified to pH 2.5 and analyzed by high-temperature combustion on a Shimadzu TOC-L. DOC was calculated as the mean of between three and five injections using a six-point standard curve.</p>
<p>Lignin-derived phenols were determined following Yan and Kaiser (2018; Anal. Chem), and Yan and Kaiser (2018; Analytica Chimica Acta). Briefly, C18 extracts were redissolved in 200 μL of 1.1 mol L−1 argon- sparged (10 min) NaOH in a 400 uL Teflon vial (Savillex Corp) and amended with containing 500 mg CuO, and amended with 10μL of10 mmolL−1 CuSO4 and 10μ L of 0.2 molL−1 ascorbic acid. Oxidation was at 150 C for 120 minutes. Following oxidation, the samples were spiked with with a surrogate standard mixture of p- hydroxybenzoic acid-13C7, vanillin-13C6, and syringaldehyde-13C6 and acidified to pH ≈ 2.5 with 6 mol L−1 sulfuric acid in the reaction vials.&nbsp; Clean-up of samples was performed with Waters HLB cartrides and final sample eluates were dried under ultra-high purity argon. Phenols were quantified by liquid chromatography/electrospray ionization-tandem mass spectrometry using a five-point calibration curve bracketing the concentration range. Quantified phenols: TDLP included vanillin, acetovanillone, vanillic acid, syringaldehyde, acetosyringone, syringic acid, coumaric acid, ferulic acid, p-hydroxy-benzaldehyde, p-hydroxy-acetophenone, and p-hydroxy-benzoic acid.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536506 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536506
completed
Karl Kaiser
Texas A&M, Galveston
409-740-4879
1001 Texas Clipper Rd Dept. of Marine Sciences, Texas A&M University, Galveston Campus
Galveston
TX
77554
USA
kaiserk@tamug.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
Cast
Bottle
Date_Time_UTC
Latitude
Longitude
Depth_water
Temp
Salinity
DOC
Phenols
liquid chromatography/electrospray ionization-tandem mass spectrometry
Niskin bottles
Shimadzu TOC-L
theme
None, User defined
station
cast
bottle
No BCO-DMO term
latitude
longitude
depth
water temperature at measurement depth
salinity
dissolved organic Carbon
featureType
BCO-DMO Standard Parameters
Mass Spectrometer
Niskin bottle
Shimadzu TOC-L Analyzer
instrument
BCO-DMO Standard Instruments
ARK-XXIX_3
service
Deployment Activity
Arctic Ocean, Central Basin
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Development and application of a high sensitivity, ultra low volume method to measure biomarkers of terrigenous organic matter in the open ocean
https://osprey.bco-dmo.org/project/763579
Development and application of a high sensitivity, ultra low volume method to measure biomarkers of terrigenous organic matter in the open ocean
<p><em>NSF Award Abstract:</em><br />
The distribution and fate of land-derived, or terrigenous, organic matter in the ocean has long been of interest to oceanographers, but that interest has grown considerably as research on the marine and global carbon cycle intensifies. Lignin is a major structural polymer found only in vascular plants, making lignin a unique tracer of terrigenous organic matter input to the marine environment. The current analytical tool for analyzing lignin, breaking it into a suite of identifiable phenolic compounds, is complex, time consuming and requires 10 to 30 liters of water. Given these limitations, applications of lignin phenols as tracers of terrestrial organic carbon in the ocean have been sparse. Through this project, the researchers aim to redesign existing chemical methodology together with modified instrumental detection for even 3 times greater sensitivity using a sample of less than 200 milliliters. Outfitting the scientific community with new methodology to sensitively trace this marker of terrigenous organic carbon will provide a clearer understanding of organic matter fluxes between and within terrestrial and oceanic reservoirs, and potentially establish lignin phenols as a robust oceanographic tracer. This project will support the development of the next generation of scientists, including an early career investigator, and graduate and undergraduate students.</p>
<p>Lignin phenol measurements have been used to study general distribution patterns and mechanisms of decomposition of terrigenous dissolved organic carbon (tDOC) in the global ocean. The distribution pattern of tDOC among ocean basins is generally consistent with the global pattern of riverine discharge to the ocean basins. However, large scale generalizations required and more fully resolved distributions of lignin as a tracer of tDOC are hampered by the difficulties and limitations associated with the present lignin phenol method. The main objectives of this project are to (1) develop methodology for measuring dissolved lignin in ultra-low volumes at high sensitivity in open ocean seawater and (2) apply the new method to study terrigenous tDOC processing and transport in the Eurasian Basin of the Arctic Ocean, where large Siberian rivers deliver the bulk of tDOC to the shelf areas. Results from this research will help evaluate lignin phenols as robust oceanographic tracers, useful to study physical mixing in the Arctic Ocean and potentially improve our understanding of the fate and removal of terrigenous organic carbon in the oceans. Addressing the second objective would provide well-constrained decay constants for lignin and tDOC in the Arctic Ocean and provide novel information on halocline formation. Integration of tDOC budgets, freshwater budgets, and circulation and atmospheric patterns will ultimately improve understanding of biogeochemical cycles in the Arctic Ocean and its role in global climate.</p>
Lignin phenol method development
largerWorkCitation
project
eng; USA
oceans
Arctic Ocean, Central Basin
-179.9473
179.8463
73.2525
89.1667
2015-08-20
2015-10-07
Arctic Ocean
0
BCO-DMO catalogue of parameters from Concentrations of dissolved organic carbon and phenols from Polarstern cruise PS 94-ARK-XXIX/3 from August to October 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/767312.rdf
Name: Station
Units: unitless
Description: <p>Station</p>
http://lod.bco-dmo.org/id/dataset-parameter/767313.rdf
Name: Cast
Units: unitless
Description: <p>Cast</p>
http://lod.bco-dmo.org/id/dataset-parameter/767314.rdf
Name: Bottle
Units: unitless
Description: <p>Bottle</p>
http://lod.bco-dmo.org/id/dataset-parameter/767315.rdf
Name: Date_Time_UTC
Units: unitless
Description: <p>Date and time (UTC); format: yyyy-mm-ddTHH:MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/767316.rdf
Name: Latitude
Units: degrees
Description: <p>Latitude north</p>
http://lod.bco-dmo.org/id/dataset-parameter/767317.rdf
Name: Longitude
Units: degrees
Description: <p>Longitude east (postive values = east)</p>
http://lod.bco-dmo.org/id/dataset-parameter/767318.rdf
Name: Depth_water
Units: meters (m)
Description: <p>Sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/767319.rdf
Name: Temp
Units: degrees Celsius
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/767320.rdf
Name: Salinity
Units: PSU
Description: <p>Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/767321.rdf
Name: DOC
Units: micromoles per liter (umol/L)
Description: <p>DOC</p>
http://lod.bco-dmo.org/id/dataset-parameter/767322.rdf
Name: Phenols
Units: picomoles per liter (pmol/L)
Description: <p>Phenols</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
9445
https://darchive.mblwhoilibrary.org/bitstream/1912/24195/1/dataset-767285_dissolved-phenols__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.767285.1
download
onLine
dataset
<p>Samples were filtered straight from Niskin bottles following established protocols for trace-metal clean sampling (Sample handling protocols for GEOTRACES cruises).</p>
<p>Samples for DOC concentration were acidified to pH 2.5 and analyzed by high-temperature combustion on a Shimadzu TOC-L. DOC was calculated as the mean of between three and five injections using a six-point standard curve.</p>
<p>Lignin-derived phenols were determined following Yan and Kaiser (2018; Anal. Chem), and Yan and Kaiser (2018; Analytica Chimica Acta). Briefly, C18 extracts were redissolved in 200 μL of 1.1 mol L−1 argon- sparged (10 min) NaOH in a 400 uL Teflon vial (Savillex Corp) and amended with containing 500 mg CuO, and amended with 10μL of10 mmolL−1 CuSO4 and 10μ L of 0.2 molL−1 ascorbic acid. Oxidation was at 150 C for 120 minutes. Following oxidation, the samples were spiked with with a surrogate standard mixture of p- hydroxybenzoic acid-13C7, vanillin-13C6, and syringaldehyde-13C6 and acidified to pH ≈ 2.5 with 6 mol L−1 sulfuric acid in the reaction vials.&nbsp; Clean-up of samples was performed with Waters HLB cartrides and final sample eluates were dried under ultra-high purity argon. Phenols were quantified by liquid chromatography/electrospray ionization-tandem mass spectrometry using a five-point calibration curve bracketing the concentration range. Quantified phenols (TDLP included vanillin, acetovanillone, vanillic acid, syringaldehyde, acetosyringone, syringic acid, coumaric acid, ferulic acid, p-hydroxy-benzaldehyde, p-hydroxy-acetophenone, and p-hydroxy-benzoic acid.</p>
<p>Samples were filtered straight from Niskin bottles following established protocols for trace-metal clean sampling (Sample handling protocols for GEOTRACES cruises)</p>
<p>Samples for DOC concentration were acidified to pH 2.5 and analyzed by high-temperature combustion on a Shimadzu TOC-L. DOC was calculated as the mean of between three and five injections using a six-point standard curve.</p>
<p>Lignin-derived phenols were determined following Yan and Kaiser (2018; Anal. Chem), and Yan and Kaiser (2018; Analytica Chimica Acta). Briefly, C18 extracts were redissolved in 200 μL of 1.1 mol L−1 argon- sparged (10 min) NaOH in a 400 uL Teflon vial (Savillex Corp) and amended with containing 500 mg CuO, and amended with 10μL of10 mmolL−1 CuSO4 and 10μ L of 0.2 molL−1 ascorbic acid. Oxidation was at 150 C for 120 minutes. Following oxidation, the samples were spiked with with a surrogate standard mixture of p- hydroxybenzoic acid-13C7, vanillin-13C6, and syringaldehyde-13C6 and acidified to pH ≈ 2.5 with 6 mol L−1 sulfuric acid in the reaction vials.&nbsp; Clean-up of samples was performed with Waters HLB cartrides and final sample eluates were dried under ultra-high purity argon. Phenols were quantified by liquid chromatography/electrospray ionization-tandem mass spectrometry using a five-point calibration curve bracketing the concentration range. Quantified phenols: TDLP included vanillin, acetovanillone, vanillic acid, syringaldehyde, acetosyringone, syringic acid, coumaric acid, ferulic acid, p-hydroxy-benzaldehyde, p-hydroxy-acetophenone, and p-hydroxy-benzoic acid.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing: modified parameter names (removed units; replaced spaces and slashes with underscores).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
liquid chromatography/electrospray ionization-tandem mass spectrometry
liquid chromatography/electrospray ionization-tandem mass spectrometry
PI Supplied Instrument Name: liquid chromatography/electrospray ionization-tandem mass spectrometry Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Shimadzu TOC-L
Shimadzu TOC-L
PI Supplied Instrument Name: Shimadzu TOC-L Instrument Name: Shimadzu TOC-L Analyzer Instrument Short Name:Shimadzu TOC-L Instrument Description: A Shimadzu TOC-L Analyzer measures DOC by high temperature combustion method.
Developed by Shimadzu, the 680 degree C combustion catalytic oxidation method is now used worldwide. One of its most important features is the capacity to efficiently oxidize hard-to-decompose organic compounds, including insoluble and macromolecular organic compounds. The 680 degree C combustion catalytic oxidation method has been adopted for the TOC-L series.
http://www.shimadzu.com/an/toc/lab/toc-l2.html Community Standard Description: http://onto.nerc.ac.uk/CAST/124.html
Cruise: ARK-XXIX_3
ARK-XXIX_3
Community Standard Description
International Council for the Exploration of the Sea
R/V Polarstern
vessel
ARK-XXIX_3
http://datadocs.bco-dmo.org/docs/Lignin_phenol_method_development/data_docs/BzPM_0703_2016.pdf
Report describing ARK-XXIX_3
Community Standard Description
International Council for the Exploration of the Sea
R/V Polarstern
vessel