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Sample collection:<\/strong><\/p>\n Each lake was sampled using the point intercept transect method at no less than 10 randomly chosen sites around its perimeter (unless the small size of a lake precluded this number of non-overlapping sites).\u00a0 At each site, three parallel transects approximately were run 5 m apart from the intertidal (0 m) to the deepest depth accessible to SCUBA divers (i.e. the bottom of the lake, or bottom of the epilimnion, or the divers\u2019 maximum certified depth).\u00a0 In lakes 8m or deeper, a line (\u2018the horizontal\u2019) was placed, at eight evenly spaced target depths (1\u20134 m depth intervals, depending on lake), orthogonal to each of the transect lines so that small (2.0 cm diameter) cells fell over four points A\u2013D each at 15 cm increments to the right of the transect line at the same depth.\u00a0 At each depth, from deepest to shallowest, the actual depth was measured in feet with a dive computer, the \u2018horizontal\u2019 was photographed from ~0.5 m distance, the substrate type was recorded, and then each cell photographed in close-up.\u00a0 A tissue sample of the \u2018primary\u2019 organism within each cell, i.e. the organism at the center of the cell, or if no organism in the centre then the first organisms at the periphery going clockwise from noon, or if only sediment visible, the organism within the sediment directly under the Cell was then biopsied for DNA analyses and placed in a container labeled with site, depth, and cell code A\u2013D.\u00a0 Because the benthos may be three dimensional a \u2018primary\u2019 organism might also have many \u2018secondary\u2019 epibionts and\/or epiphytes attached.\u00a0 Any organisms in the photographs but not sampled were classified as \u2018tertiary\u2019.\u00a0 After all four cells were sampled at a depth, the diver ascended to the next shallowest depth on his\/her transect and repeated the procedure.\u00a0 Thus, at each randomly chosen site, we surveyed a total of 96 points from the deepest to shallowest depths of the lake habitable by macro-invertebrates and macrophytes, with two categories of exception. (1) If a lake was <8 m deep, the number of depths sampled was equivalent to the maximum depth in meters. (2) Lakes with gently sloping sides could lead to adjacent target depths being >10 m apart leading to undersampling of horizontal patchiness; in which case the transect distance between adjacent target depths was estimated and divided in half or in thirds so that no two samples were more than 10 transect meters apart.\u00a0 At the surface, at the end of each dive, samples were transferred to individual tubes of 95% ethanol labeled with a field number composed of lake, site, collector, depth, and cell IDs.\u00a0 Each evening, new samples were stored in a freezer, dive profiles were downloaded, and fieldnotes were transcribed to a standardized electronic data sheet.<\/p>\n Error-checking biodiversity transect files<\/strong><\/p>\n Each evening, or as soon thereafter as possible, divers compared specimens to standardize field-identifications and all tissue samples were reconciled to the electronic data sheet for each lake using tube labels, original field notes, photographs of specimens in the field, and visual inspection of tube contents.\u00a0 If necessary, primary and secondary specimens were placed in individually labelled tubes of ethanol.\u00a0 In cases of discrepancy between electronic notes and original field notes, we edited the electronic data sheet to be consistent with original notes and corroborated this by double-checking the original photographs and inspecting tube contents.\u00a0 Significant changes\u2014i.e. samples that could not be reconciled after accounting for tube transpositions, mislabeling, or mis-identification in the field\u2014were logged in a separate file highlighting the specific change and justification.\u00a0 If a specimen was unable to be reconciled with notes it was discarded (this was necessary for only one specimen).\u00a0 Subsequently, every tissue sample was assigned an unique identifying number (M0D#) for curation; during this process, every tenth sample was double-checked for agreement between the original field number and new M0D#.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Palau lakes: tissue archive","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":" Marine lakes of Palau barcoded specimens from transect survey with both lab identification number (M0D#) and original tube number. 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The purpose of this dataset is (1) to link unique identification numbers used in different circumstances and files, specifically tube ID used in the field and the M0D# identifier for the lab\u2019s permanent collection, and (2) to summarize the tissue samples collected during biodiversity surveys under this project.","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/temporalExtent":[{"@value":"_:temporalExtent768180"}],"http:\/\/ocean-data.org\/schema\/spatialCoverage":[{"@value":"_:spatialCoverage768180"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"Dawson, M. (2019) Marine lakes of Palau barcoded specimens from transect survey with both lab identification number (M0D#) and original tube number. Biological and Chemical Oceanography Data Management Office (BCO-DMO). 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