http://lod.bco-dmo.org/id/dataset/769203
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-05-31
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Concentration measurements of water column phosphate, nitrate and nitrite, dissolved organic phosphorus, methane, and ethylene from samples collected during the R/V Neil Armstrong cruise AR16 in the western North Atlantic Ocean in May 2017
2019-07-19
publication
2019-07-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-07-22
publication
https://doi.org/10.1575/1912/bco-dmo.769203.2
Daniel J. Repeta
Woods Hole Oceanographic Institution
principalInvestigator
David M. Karl
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Repeta, D., Karl, D. (2019) Concentration measurements of water column phosphate, nitrate and nitrite, dissolved organic phosphorus, methane, and ethylene from samples collected during the R/V Neil Armstrong cruise AR16 in the western North Atlantic Ocean in May 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2019-07-19 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.769203.2 [access date]
Dataset Description: <p>Concentration measurements of water column phosphate, nitrate and nitrite, dissolved organic phosphorus, methane, and ethylene from samples collected during the R/V Niel Armstrong cruise AR16 in the western North Atlantic Ocean in May 2017.</p> Methods and Sampling: <p>Sampling was conducted aboard the R/V Neil Armstrong (cruise AR16) in May 2017. Seawater was collected from Niskin bottles deployed on a rosette with a CTD.</p>
<p>Samples for seawater methane (CH4) and ethylene (C2H4) concentration measurements were collected from the CTD rosette in 250 mL glass serum vials (pre-combusted) crimp-sealed with aluminum collars and with Teflon-lined septa. Samples were typically analyzed the same day of sampling aboard the R/V Armstrong with an Agilent 7980A gas chromatograph equipped with a flame ionization detector (FID) and a gas stripping and cryo-trap concentration method as described previously (Repeta et al. 2016). The FID was calibrated by injecting different sized loops of a gas mixture standard containing 10 ppm of CH4 and C2H4 in pure nitrogen gas (Scott-Marrin, Riverside, CA, USA).</p>
<p>Samples for nutrient analyses were collected in acid-clean HDPE plastic bottles. Samples were shipped frozen to the Center for Microbial Oceanography: Research and Education at the University of Hawaii at Manoa where they were stored frozen until analysis.</p>
<p>Seawater phosphate (PO4) concentrations were determined following the autoanalyzer colorimetric procedure of Foreman et al. (2019). Seawater nitrate+nitrite (NOx) concentrations were determined following the autoanalyzer colorimetric procedure outlined of Foreman et al. (2016). Both PO4 and NOx analyses were performed on a SEAL Analytics autoanalyzer model 3 HR.</p>
<p>Seawater samples with low level N+N (LLN) concentrations were measured using the modified chemiluminescent method based on titanium (III) trichloride reduction of N+N to nitric oxide gas and detection with an Antek model 7090 as described by Foreman et al. (2016).</p>
<p>MAGIC soluble reactive phosphorus (SRP) concentrations (i.e. phosphate) were determined in seawater samples using the MAGnesium Induced Coprecipitation (MAGIC) technique as described by Karl and Tien (1992) with modifications based on Thompson-Bulldis and Karl (1998) and Cavendar-Bares et al. (2001) to increase the concentration factor of phosphate. Specifically, SRP was concentrated from 150 mL sweater samples using 0.75 mL (0.5% v/v) of 1M NaOH to precipitate Mg(OH)₂ and the Mg(OH)₂ pellets were dissolved with 5.25 mL of 0.1M HCl.</p>
<p>Total dissolved P (TDP) concentrations were measured by a photo-oxidation procedure in which controlled exposure to ultraviolet (UV) radiation converts organic P in seawater to phosphate which is then measured by the modified molybdenum blue method adapted for the autoanalyzer technique. The UV photo-oxidation methodology for TDP analysis is described in Foreman et al. (2019). The difference between TDP and PO4 or TDP and SRP is calculated to estimate the concentration of dissolved organic phosphorus (DOP) in seawater.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1634080 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1634080
completed
Daniel J. Repeta
Woods Hole Oceanographic Institution
508-289-2635
360 Woods Hole Rd. MS #51 Watson Laboratory
Woods Hole
MA
02543
USA
drepeta@whoi.edu
pointOfContact
David M. Karl
University of Hawaiʻi at Mānoa
808-956 8964
C-MORE Hale 1950 East-West Road
Honolulu
HI
96822
USA
dkarl@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
Cruise
Station
Cast
Latitude
Longitude
ISO_DateTime_UTC
Depth
CH4
C2H4
NOx
LLN
PO4
SRP
TDP
Antek model 7090
Agilent 7980A
Agilent 7980A
Niskin bottles
SEAL Analytics autoanalyzer model 3 HR
theme
None, User defined
Cruise identfier
station
cast
latitude
longitude
ISO_DateTime_UTC
depth
methane
No BCO-DMO term
nitrate plus nitrite
Low Level Nitrogen
reactive phosphorus (PO4)
SRP
Total Dissolved Phosphorus
featureType
BCO-DMO Standard Parameters
Chemiluminescence NOx Analyzer
Flame Ionization Detector
Gas Chromatograph
Niskin bottle
Seal Analytical AutoAnalyser 3HR
instrument
BCO-DMO Standard Instruments
AR16
service
Deployment Activity
Western North Atlantic
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Methane, Ethylene, and Dissolved Organic Phosphorus Cycling in the Western North Atlantic Ocean
https://osprey.bco-dmo.org/project/765014
Methane, Ethylene, and Dissolved Organic Phosphorus Cycling in the Western North Atlantic Ocean
<p><em>NSF Award Abstract:</em><br />
The "marine methane paradox" refers to observations of high concentrations of methane in surface waters of the ocean, even though these waters are well-oxygenated and high in sulfate, both features which generally do not favor the production of methane. This project aims to elucidate on the cause of this paradox. Based on preliminary results, the investigator identified the potential for methane production stimulated by microbial cycling of dissolved organic matter. Polysaccharides (carbonates such as starch) are a major component of high molecular weight dissolved organic matter (HMWDOM) and they can incorporate esters of methylphosphonate, which may be allowing for the production of methane in surface waters. When these HMWDOM polysaccharides were introduced to seawater samples, the researchers saw production of methane with an anomalous stable carbon isotope ratio comparable to what is seen in the paradoxical surface waters enriched in methane. By making measurements of trace gases, HMWDOM phosphonate, and stable carbon isotope measurements in the western North Atlantic Ocean, the investigator and his collaborators will reassess the preliminary data and the implications of these results for the entire oceanographic cycling of methane. Both educational and outreach efforts have been included. For the educational component, a postdoc, a graduate student, and summer undergraduate students will be work on the project, whereas for the outreach activities, the proponent plans to develop curricula on basic science and oceanography for high school students, include a high school teacher in the research cruise, and integrate results into an organic geochemistry course, the lectures of which will be publically available online.</p>
<p>This project seeks to evaluate the possibility that the "marine methane paradox", or the super-saturation of methane in high sulfate, well-oxygenated surface waters, is caused by microbial cycling of dissolved organic matter (DOM). There has been a great deal of preliminary evidence to support this theory. For example, samples from station ALOHA were amended with purified high molecular weight DOM (HMWDOM) polysaccharides, and methane, ethylene, and propylene productions were stimulated. HMWDOM polysaccharides incorporate esters of methylphosphonate (MPn), 2-hydroxyethylphosphonates (2-HEP), and other minor phosphonates, which are likely facilitating the production of methane, ethylene, and propylene. Carbon isotope data supports that this theory is the process behind the marine methane paradox; the d13C value of methane produced from HMWDOM polysaccharide enriched samples agrees well with the anomalous value associated with the super-saturated surface water. Only a small fraction of HMWDOM polysaccharides can easily explain the marine methane paradox, and this process could just as easily fully revise the current understanding of large scale methane cycling between the ocean and atmosphere. The researchers will assess the preliminary evidence and evaluate the microbial DOM cycling theory by making paired trace gas and HMWDOM phosphonate, as well as stable carbon isotope measurements in the western North Atlantic Ocean.</p>
PHAT
largerWorkCitation
project
eng; USA
oceans
Western North Atlantic
-71.33867
-64.1875
29.05817
40.4025
2017-05-04
2017-05-20
Sargasso Sea, Western North Atlantic Ocean
0
BCO-DMO catalogue of parameters from Concentration measurements of water column phosphate, nitrate and nitrite, dissolved organic phosphorus, methane, and ethylene from samples collected during the R/V Neil Armstrong cruise AR16 in the western North Atlantic Ocean in May 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/769226.rdf
Name: Cruise
Units: unitless
Description: <p>cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/769227.rdf
Name: Station
Units: unitless
Description: <p>station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/769228.rdf
Name: Cast
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/769229.rdf
Name: Latitude
Units: decimal degrees
Description: <p>latitude north (positive values = North)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769230.rdf
Name: Longitude
Units: decimal degrees
Description: <p>longitude east (negative values = West)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769231.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>date and time (UTC) formatted to ISO8601 standard; format: yyyy-mm-ddTHH:MM:SS</p>
http://lod.bco-dmo.org/id/dataset-parameter/769232.rdf
Name: Depth
Units: meters (m)
Description: <p>sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/769233.rdf
Name: CH4
Units: nanomoles per liter (nmol L-1)
Description: <p>methane</p>
http://lod.bco-dmo.org/id/dataset-parameter/769234.rdf
Name: C2H4
Units: nanomoles per liter (nmol L-1)
Description: <p>ethylene</p>
http://lod.bco-dmo.org/id/dataset-parameter/769235.rdf
Name: NOx
Units: micromoles per liter (umol L-1)
Description: <p>nitrate + nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/769236.rdf
Name: LLN
Units: micromoles per liter (umol L-1)
Description: <p>low level nitrogen (nitrate + nitrite)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769237.rdf
Name: PO4
Units: micromoles per liter (umol L-1)
Description: <p>phosphate</p>
http://lod.bco-dmo.org/id/dataset-parameter/769238.rdf
Name: SRP
Units: micromoles per liter (umol L-1)
Description: <p>soluble reactive phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/769239.rdf
Name: TDP
Units: micromoles per liter (umol L-1)
Description: <p>total dissolved phosphorus</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
9132
https://darchive.mblwhoilibrary.org/bitstream/1912/24359/1/dataset-769203_water-column-methane-ethylene-and-nutrients__v2.tsv
download
https://doi.org/10.1575/1912/bco-dmo.769203.2
download
onLine
dataset
<p>Sampling was conducted aboard the R/V Neil Armstrong (cruise AR16) in May 2017. Seawater was collected from Niskin bottles deployed on a rosette with a CTD.</p>
<p>Samples for seawater methane (CH4) and ethylene (C2H4) concentration measurements were collected from the CTD rosette in 250 mL glass serum vials (pre-combusted) crimp-sealed with aluminum collars and with Teflon-lined septa. Samples were typically analyzed the same day of sampling aboard the R/V Armstrong with an Agilent 7980A gas chromatograph equipped with a flame ionization detector (FID) and a gas stripping and cryo-trap concentration method as described previously (Repeta et al. 2016). The FID was calibrated by injecting different sized loops of a gas mixture standard containing 10 ppm of CH4 and C2H4 in pure nitrogen gas (Scott-Marrin, Riverside, CA, USA).</p>
<p>Samples for nutrient analyses were collected in acid-clean HDPE plastic bottles. Samples were shipped frozen to the Center for Microbial Oceanography: Research and Education at the University of Hawaii at Manoa where they were stored frozen until analysis.</p>
<p>Seawater phosphate (PO4) concentrations were determined following the autoanalyzer colorimetric procedure of Foreman et al. (2019). Seawater nitrate+nitrite (NOx) concentrations were determined following the autoanalyzer colorimetric procedure outlined of Foreman et al. (2016). Both PO4 and NOx analyses were performed on a SEAL Analytics autoanalyzer model 3 HR.</p>
<p>Seawater samples with low level N+N (LLN) concentrations were measured using the modified chemiluminescent method based on titanium (III) trichloride reduction of N+N to nitric oxide gas and detection with an Antek model 7090 as described by Foreman et al. (2016).</p>
<p>MAGIC soluble reactive phosphorus (SRP) concentrations (i.e. phosphate) were determined in seawater samples using the MAGnesium Induced Coprecipitation (MAGIC) technique as described by Karl and Tien (1992) with modifications based on Thompson-Bulldis and Karl (1998) and Cavendar-Bares et al. (2001) to increase the concentration factor of phosphate. Specifically, SRP was concentrated from 150 mL sweater samples using 0.75 mL (0.5% v/v) of 1M NaOH to precipitate Mg(OH)₂ and the Mg(OH)₂ pellets were dissolved with 5.25 mL of 0.1M HCl.</p>
<p>Total dissolved P (TDP) concentrations were measured by a photo-oxidation procedure in which controlled exposure to ultraviolet (UV) radiation converts organic P in seawater to phosphate which is then measured by the modified molybdenum blue method adapted for the autoanalyzer technique. The UV photo-oxidation methodology for TDP analysis is described in Foreman et al. (2019). The difference between TDP and PO4 or TDP and SRP is calculated to estimate the concentration of dissolved organic phosphorus (DOP) in seawater.</p>
Specified by the Principal Investigator(s)
<p>nd = not determined; bdl = below detection limit.</p>
<p>BCO-DMO Processing:<br />
-&nbsp;Re-formatted original date/time field into ISO8601 format;<br />
- 2019-07-19: served version 2 of data (detection limits of ethylene gas were re-calculated).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Antek model 7090
Antek model 7090
PI Supplied Instrument Name: Antek model 7090 Instrument Name: Chemiluminescence NOx Analyzer Instrument Short Name: Instrument Description: The chemiluminescence method for gas analysis of oxides of nitrogen relies on the measurement of light produced by the gas-phase titration of nitric oxide and ozone. A chemiluminescence analyzer can measure the concentration of NO/NO2/NOX.
One example is the Teledyne Model T200: https://www.teledyne-api.com/products/nitrogen-compound-instruments/t200
Agilent 7980A
Agilent 7980A
PI Supplied Instrument Name: Agilent 7980A Instrument Name: Flame Ionization Detector Instrument Short Name:FID Instrument Description: A flame ionization detector (FID) is a scientific instrument that measures the concentration of organic species in a gas stream. It is frequently used as a detector in gas chromatography. Standalone FIDs can also be used in applications such as landfill gas monitoring, fugitive emissions monitoring and internal combustion engine emissions measurement in stationary or portable instruments.
Agilent 7980A
Agilent 7980A
PI Supplied Instrument Name: Agilent 7980A Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
SEAL Analytics autoanalyzer model 3 HR
SEAL Analytics autoanalyzer model 3 HR
PI Supplied Instrument Name: SEAL Analytics autoanalyzer model 3 HR Instrument Name: Seal Analytical AutoAnalyser 3HR Instrument Short Name:Seal Analytical AutoAnalyser 3HR Instrument Description: A fully automated Segmented Flow Analysis (SFA) system, ideal for water and seawater analysis. It comprises a modular system which integrates an autosampler, peristaltic pump, chemistry manifold and detector. The sample and reagents are pumped continuously through the chemistry manifold, and air bubbles are introduced at regular intervals forming reaction segments which are mixed using glass coils. The AA3 uses segmented flow analysis principles to reduce inter-sample dispersion, and can analyse up to 100 samples per hour using stable LED light sources.
Cruise: AR16
AR16
International Council for the Exploration of the Sea
R/V Neil Armstrong
vessel
AR16
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
International Council for the Exploration of the Sea
R/V Neil Armstrong
vessel