<div><p>Sampling was conducted with a 20 micron plankton net on 12/30/2005 at about 1am (local time), near station 137, was extracted for total protein using an SDS detergent method followed by tube gel purification, reduction, alkylation, and tryptic digestion and analyzed on a Thermo Orbitrap Fusion using 2-dimensional separation scheme to maximize metaproteome depth. Detailed methods available in Bender et al. 2018.</p></div>
<div><p>These data are part of the Ocean Protein Portal "Ross Sea Net Tow (Bender)" dataset version 1 (<a href="https://proteinportal.whoi.edu/;">https://proteinportal.whoi.edu/;</a> Saito et al., 2019).</p></div>
Ross Sea metaproteome FASTA files
<div><p>Mass spectra from 2D LC-MS was peptide-to-spectrum matched using the SEQUEST algorithm within Proteome Discoverer software, followed by spectral counting with Proteome Software's Scaffold software using the FASTA generated from Phaeocystis culture transcriptome study. Data processing documented in Bender et al. 2018.</p></div>
769266
Ross Sea metaproteome FASTA files
2019-06-03T14:51:56-04:00
2019-06-03T14:51:56-04:00
2024-03-21T14:37:00-04:00
urn:bcodmo:dataset:769266
FASTA file of protein identifications from net tows during RVIB Nathaniel B. Palmer cruise NBP0601 in December of 2015
A net tow metaproteome of a Phaeocystis antarctica bloom in the Ross Sea, mapped here to Phaeocystis metatranscriptomes analyzed by 2D LCMS, in units of protein spectral counts.
false
Saito, M. A. (2019) FASTA file of protein identifications from net tows during RVIB Nathaniel B. Palmer cruise NBP0601 in December of 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-05-17 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/769266 [access date]
false
1
false
metaproteomics
Ross Sea
Phaeocystis antarctica
Antartica
2019-05-17
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2005-12-30
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363
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OSPREY
http://www.opengis.net/def/crs/OGC/1.3/CRS84
<http://www.opengis.net/def/crs/OGC/1.3/CRS84> POINT (170.76 -76.82)
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