http://lod.bco-dmo.org/id/dataset/769266
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-06-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
FASTA file of protein identifications from net tows during RVIB Nathaniel B. Palmer cruise NBP0601 in December of 2015
2019-05-17
publication
2019-05-17
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2024-05-23
publication
https://doi.org/10.26008/1912/bco-dmo.769266.1
Mak A. Saito
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Saito, M. A. (2024) FASTA file of protein identifications from net tows during RVIB Nathaniel B. Palmer cruise NBP0601 in December of 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-05-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.769266.1 [access date]
Dataset Description: <p>These data are part of the Ocean Protein Portal "Ross Sea Net Tow (Bender)" dataset version 1&nbsp;(<a href="https://proteinportal.whoi.edu/;">https://proteinportal.whoi.edu/;</a>&nbsp;Saito et al., 2020).</p> Methods and Sampling: <p>Sampling was conducted with a 20 micron plankton net on 12/30/2005 at about 1am (local time), near station 137, was extracted for total protein using an SDS detergent method followed by tube gel purification, reduction, alkylation, and tryptic digestion and analyzed on a Thermo Orbitrap Fusion using 2-dimensional separation scheme to maximize metaproteome depth. Detailed methods available in Bender et al. 2018.<br />
<br />
This .fasta file is&nbsp; a standard FASTA format for sequences with first line of entry starting with "&gt;" and a text description, followed by the associated amino acid sequence. This file is unitless (all sequence, no integers).&nbsp;<br />
<br />
Organism identifier (LifeSciences Identifier, LSID):<br />
Phaeocystis antarctica, urn:lsid:marinespecies.org:taxname:341585</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-0338097 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0338097
completed
Mak A. Saito
Woods Hole Oceanographic Institution
508-289-2393
266 Woods Hole Rd. MS #51 Watson Lab
Woods Hole
MA
02543
USA
msaito@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Thermo Scientific Q-Exactive Orbitrap mass spectrometer with a Michrom Advance CaptiveSpray source
theme
None, User defined
Mass Spectrometer
instrument
BCO-DMO Standard Instruments
NBP0601
service
Deployment Activity
Ross Sea Southern Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
Controls of Ross Sea Algal Community Structure
http://www.whoi.edu/sites/corsacs
Controls of Ross Sea Algal Community Structure
<h3>Project summary</h3>
<p>The Controls of Ross Sea Algal Community Structure (CORSACS) project was funded by the NSF Office of Polar Programs as "Collaborative Research: Interactive Effects of Iron, Light and Carbon Dioxide on Phytoplankton Community Dynamics in the Ross Sea". Two cruises were completed in 2006 to investigate the interactions between the primary productivity of the Ross Sea and pCO2, iron and other trace elements. Data sets of carbon, nutrient, metal, and biological measurements will be reported.</p>
<p>The main objective in the proposed research was to investigate the relative importance and potential interactive effects of iron, light and CO2 levels in structuring algal assemblages and growth rates in the Ross Sea. The investigators hypothesized that the interaction of these three variables largely determines the bottom-up control on these two dominant Southern Ocean phytoplankton taxa. While grazing and other loss processes are important variables in determining the relative dominance of these two taxa, the CORSACS research project was designed to focus on the bottom-up control mechanisms. It is important to understand such environmentally-driven taxonomic shifts in primary production, since they are expected to impact the fixation and export of carbon and nutrients, and the production of DMS, thus potentially providing both positive and negative feedbacks on climate.</p>
<p>The CORSACS investigators considered a range of ambient iron, light and pCO2 levels that span those typically observed in the Ross Sea during the growing season. That is, dissolved iron ranging from ~0.1 nM (low iron) to greater than 1 nM (high iron) (Fitzwater et al. 2000; Sedwick et al. 2000); mean irradiance (resulting from vertical mixing/self shading) ranging from less than 10% Io (low light) to greater than 40% (high light) (Arrigo et al., 1998, 1999), possibly adjusted based on field observations during the CORSACS cruises; and pCO2 ranging (Sweeney et al. 2001) from ~150 ppm (low CO2) to the probable higher levels of pCO2 - 750 ppm as a conservative estimate - that are likely to be attained later this century due to anthropogenic perturbation of the global carbon cycle (IPCC, 2001).</p>
<p>From the information previously available from both field observations and experiments, the investigators formulated the following specific hypotheses regarding the interactive role of iron, light and CO2 in regulating algal composition in the Ross Sea: diatoms bloom in the southern Ross Sea only under optimum conditions of high iron, light and pCO2; colonial Phaeocystis dominate under conditions of high iron with either (or both) low light or low pCO2; and solitary Phaeocystis are predominant under conditions of low iron with either (or both) low light or low pCO2.</p>
<h4>References:</h4>
<p>Fitzwater, S.E., K.S. Johnson, R.M. Gordon, K.H. Coale, and W.O. Smith, Jr. (2000). Trace metal concentrations in the Ross Sea and their relationship with nutrients and growth. Deep-Sea Research II, 47: 3159-3179.</p>
<p>Martin JH, Gordon RM, Fitzwater SE. Iron in Antarctic waters. Nature 1990 ;345(6271):156-158. Martin JH. 1990. Glacial-interglacial CO2 change: The iron hypothesis. Paleoceanography 5(1):1-13</p>
<p>P. N. Sedwick, G. R. DiTullio, and D. J. Mackey, Iron and manganese in the Ross Sea, Antarctica: Seasonal iron limitation in Antarctic shelf waters, Journal of Geophysical Research, 105 (C5), 11,321-11,336, 2000.</p>
<p>Sweeney, C. K. Arrigo, and G. van Gijken (2001). Prediction of seasonal changes in surface pCO2 in the Ross Sea, Antarctica using ocean color satellite data. 2001 Annual AGU meeting, San Fransisco, CA Dec. 10-15.</p>
<p>IPCC, 2001: Climate Change 2001: Synthesis Report. A Contribution of Working Groups I, II, and III to the Third Assessment Report of theIntegovernmental Panel on Climate Change [Watson, R.T. and the Core Writing Team (eds.)]. Cambridge University Press, Cambridge,United Kingdom, and New York, NY, USA, 398 pp.</p>
<p><strong>Publications</strong></p>
<p>Saito, M. A., Goepfert, T. J., Noble, A. E., Bertrand, E. M., Sedwick, P. N., and DiTullio, G. R.: A seasonal study of dissolved cobalt in the Ross Sea, Antarctica: micronutrient behavior, absence of scavenging, and relationships with Zn, Cd, and P, Biogeosciences, 7, 4059-4082, doi:10.5194/bg-7-4059-2010, 2010 (<a href="http://www.biogeosciences.net/7/4059/2010/bg-7-4059-2010.html">http://www.biogeosciences.net/7/4059/2010/bg-7-4059-2010.html</a>)</p>
<p>Bertrand EM, Saito MA, Lee PA, Dunbar RB, Sedwick PN and DiTullio GR (2011) Iron limitation of a springtime bacterial and phytoplankton community in the Ross Sea: implications for vitamin B12 nutrition. Front. Microbio. 2:160. doi: 10.3389/fmicb.2011.00160 (<a href="http://www.frontiersin.org/Aquatic_Microbiology/10.3389/fmicb.2011.00160/abstract">http://www.frontiersin.org/Aquatic_Microbiology/10.3389/fmicb.2011.00160/abstract</a>)</p>
CORSACS
largerWorkCitation
project
eng; USA
oceans
Ross Sea Southern Ocean
170.76
170.76
-76.82
-76.82
2005-12-30
2005-12-30
Ross Sea Southern Ocean
0
BCO-DMO catalogue of parameters from FASTA file of protein identifications from net tows during RVIB Nathaniel B. Palmer cruise NBP0601 in December of 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
607303
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/9dae652c-0818-4d99-9290-3b1a4818ce99/content
download
https://doi.org/10.26008/1912/bco-dmo.769266.1
download
onLine
dataset
<p>Sampling was conducted with a 20 micron plankton net on 12/30/2005 at about 1am (local time), near station 137, was extracted for total protein using an SDS detergent method followed by tube gel purification, reduction, alkylation, and tryptic digestion and analyzed on a Thermo Orbitrap Fusion using 2-dimensional separation scheme to maximize metaproteome depth. Detailed methods available in Bender et al. 2018.<br />
<br />
This .fasta file is&nbsp; a standard FASTA format for sequences with first line of entry starting with "&gt;" and a text description, followed by the associated amino acid sequence. This file is unitless (all sequence, no integers).&nbsp;<br />
<br />
Organism identifier (LifeSciences Identifier, LSID):<br />
Phaeocystis antarctica, urn:lsid:marinespecies.org:taxname:341585</p>
Specified by the Principal Investigator(s)
Mass spectra from 2D LC-MS was peptide-to-spectrum matched using the SEQUEST algorithm within Proteome Discoverer software, followed by spectral counting with Proteome Software's Scaffold software using the FASTA generated from Phaeocystis culture transcriptome study. FASTA of discovered protein sequences exported from Proteome Software. Data processing was documented in Bender et al. 2018.
Specified by the Principal Investigator(s)
fasta file added to BCO-DMO data system, no changes.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Thermo Scientific Q-Exactive Orbitrap mass spectrometer with a Michrom Advance CaptiveSpray source
Thermo Scientific Q-Exactive Orbitrap mass spectrometer with a Michrom Advance CaptiveSpray source
PI Supplied Instrument Name: Thermo Scientific Q-Exactive Orbitrap mass spectrometer with a Michrom Advance CaptiveSpray source Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Cruise: NBP0601
NBP0601
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel
NBP0601
Giacomo DiTullio
College of Charleston - Hollings Marine Lab
http://data.bco-dmo.org/CORSACS/cruises/Dunbar_Hydrography_report_NBP0601.pdf
Report describing NBP0601
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel