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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/770638.rdf" xlink:actuate="onRequest">Biogeochemical and sediment characteristics of the Cinder Cones Methane seep site in the Ross Sea from November 2016</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Thurber, A. (2019) Biogeochemical and sediment characteristics of the Cinder Cones Methane seep site in the Ross Sea from November 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-06-21 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.770638.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Biogeochemical and sediment characteristics of the Cinder Cones Methane seep site Dataset Description: &amp;lt;p&amp;gt;Biogeochemical and sediment characteristics of the Cinder Cones Methane seep site&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Sediment samples: Sediment cores were collected from the Cinder Cones site including a methane seep habitat and vertically sectioned into identified intervals at either 3cm or 1cm intervals depending on the analysis. To quantify methane from sediment plugs, subcores were taken vertically via a syringe with the end cut off to sample 3 cm3 of sediment.&amp;amp;nbsp; The subcores were preserved in 2ml of 5M NaOH in a serum bottle and caped with a butyl stopper for later analysis. It was stored and shipped upside down. Large grain size led to rapid dewatering of the cores making the concentrations likely conservative estimates of the methane (i.e. the is a chance some of the porewater was lost during sampling).&amp;amp;nbsp; For 0-1 cm sediment plugs, a core was vertically sectioned and frozen at -80oC until later analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Porewater samples:&amp;amp;nbsp; Pore water was sampled in situ by placing a vertical barrier in the sediment, excavating one side and inserting a Rhyzon filter (0.2 um) into the intact sediment on the opposite side of the excavation. 1 ml of porewater was discarded as a rinse and then 5ml of pore water was extracted. These were taken at 2 cm intervals starting at 1 cm below the sediment surface to 5cm.&amp;amp;nbsp; 3ml were preserved in serum bottles with 2ml of 5M NaOH for later methane analysis. 1 ml was filtered to remove particles &amp;amp;gt;0.2 um and frozen for ion analysis and 1ml was preserved with 0.25ml of 0.05 M Zinc acetate for hydrogen sulfide concentration for Spectrophotometric analysis following Cline (1969).&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sampling and analytical procedures:&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;br /&amp;gt;
Methane: Samples were kept stored cold and inverted for both porewater and sediment plug sampling approaches for methane.&amp;amp;nbsp; Headspace from each of the serum vials was injected into a Gas Chromatograph with Flame Ionization Detector (GC-FID) after a standard curve was generated based on known standards increasing from 10 to 1000 ppm and the same injection volume (between 50 and 100 ul) as the headspace of the samples.&amp;amp;nbsp; Adjusted r2 of the calibration curve was&amp;amp;nbsp; 1.0.&amp;amp;nbsp; Response area was measured using “Peak Simple” software.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Methane Flux: Methane flux was measured in 2016 through the deployment of benthic flux chambers. Chambers were 10cm i.d. cores with a cap containing a septa sealed hole for sampling and an o-ring seal around the cap. The chambers were sealed with the caps after the cores were inserted into the sediment to ensure minimal disturbance to the system. Chambers were not continually stirred but were stirred by using magnetic propeller system that allowed gentle agitation prior to each sample collection. Samples were taken on 12-hour intervals to quantify that release of methane through syringe sampling through the septa; at no point were the chambers opened&amp;lt;/p&amp;gt;</gco:CharacterString>
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http://lod.bco-dmo.org/id/dataset-parameter/770653.rdf
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	Units: unitless
	Description: &lt;p&gt;sampling approach used for the observations (porewater; sediment; or flux chamber)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770654.rdf
	Name: Fluoride
	Units: millimole (mmol)
	Description: &lt;p&gt;Fluoride&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770655.rdf
	Name: Acetate
	Units: millimole (mmol)
	Description: &lt;p&gt;Acetate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770656.rdf
	Name: Formate
	Units: millimole (mmol)
	Description: &lt;p&gt;Formate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770657.rdf
	Name: Chloride
	Units: millimole (mmol)
	Description: &lt;p&gt;Chloride&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770658.rdf
	Name: Nitrite
	Units: millimole (mmol)
	Description: &lt;p&gt;Nitrite&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770659.rdf
	Name: Bromide
	Units: millimole (mmol)
	Description: &lt;p&gt;Bromide&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770660.rdf
	Name: Sulfate
	Units: millimole (mmol)
	Description: &lt;p&gt;Sulfate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770661.rdf
	Name: Thiosulfate
	Units: millimole (mmol)
	Description: &lt;p&gt;Thiosulfate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770662.rdf
	Name: Lithium
	Units: millimole (mmol)
	Description: &lt;p&gt;Lithium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770663.rdf
	Name: Sodium
	Units: millimole (mmol)
	Description: &lt;p&gt;Sodium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770664.rdf
	Name: Ammonium
	Units: millimole (mmol)
	Description: &lt;p&gt;Ammonium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770665.rdf
	Name: Potassium
	Units: millimole (mmol)
	Description: &lt;p&gt;Potassium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770666.rdf
	Name: Magnesium
	Units: millimole (mmol)
	Description: &lt;p&gt;Magnesium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770667.rdf
	Name: Calcium
	Units: millimole (mmol)
	Description: &lt;p&gt;Calcium&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770668.rdf
	Name: Sulfide
	Units: millimole (mmol)
	Description: &lt;p&gt;Sulfide&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770669.rdf
	Name: Methane_porewater
	Units: nanomole per milliliter of porewater (nmol ml-1 porewater)
	Description: &lt;p&gt;Methane in porewater&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770670.rdf
	Name: Methane_sediment
	Units: nanomole per cubic centimeter of sediment (nmol cm^-3 sediment)
	Description: &lt;p&gt;Methane in sediment&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770671.rdf
	Name: d2HVSMOW
	Units: per mil
	Description: &lt;p&gt;stable isotopic composition of hydrogen in comparison to the reference &quot;Vienna-Standard Mean Ocean Water&quot;&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770672.rdf
	Name: d13CVPDB
	Units: per mil
	Description: &lt;p&gt;stable isotopic composition of carbon in comparison to the reference &quot;Vienna PeeDee Belemnite&quot;&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/770673.rdf
	Name: Methane_Flux
	Units: millimole per meter squared per day (mmol m-2 d-1)
	Description: &lt;p&gt;Methane_Flux&lt;/p&gt; 
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