http://lod.bco-dmo.org/id/dataset/771370
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-06-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Phosphate, silicate, and nitrate plus nitrite measurements
2019-06-17
publication
2019-06-17
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-06-29
publication
https://doi.org/10.26008/1912/bco-dmo.771370.1
Uta Passow
University of California-Santa Barbara
principalInvestigator
Edward Laws
Louisiana State University College of the Coast and Environment
principalInvestigator
Nigel D'Souza
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Passow, U., Laws, E., D'Souza, N. (2020) Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Phosphate, silicate, and nitrate plus nitrite measurements. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-06-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.771370.1 [access date]
Series 3A: nutrients Dataset Description: <p>The experiments in Series 3A were designed to test the combined effects of three CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset reports the macronutrient (phosphate, silicate, and nitrate plus nitrite) concentrations measured during the experiments.</p> Methods and Sampling: <p>Three CO2 concentrations were tested: 410 ppm, 750 ppm, and 1000 ppm respectively. For each CO2 concentration, four temperatures were tested: 15 degrees-C, 20 degrees-C, 25 degrees-C, and 30 degrees-C. Within each temperature, three light levels were tested: a sub-optimum light (SOL) intensity of 60 umol photons · m-2 · s-1, an optimum light (OL) intensity of 400 umol photons · m-2 · s-1 and an extreme light (EL) intensity of 800 umol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series, with all light treatments at two temperatures (either 15 degrees-C and 25 degrees-C or 20 degrees-C and 30 degrees-C) running simultaneously. This was repeated for each CO2 concentration.</p>
<p>Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2um filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile artificial seawater, and the humidified gas mix was supplied to each tube via gentle sparging through a 2um stainless steel diffuser. Flow rates were gradually increased over the course of the incubation to compensate for the DIC uptake of actively growing cells, and ranged from &lt;0.04 Liters per minute (LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days. For each CO2 and temperature level, replication was achieved by incubating three tubes at sub-optimum light intensities, two tubes at optimum light intensity, and three tubes at extreme light intensities. Each experiment was split into two phases: An acclimation phase spanning 4 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 3-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 5 hours into the daily light cycle to minimize the effects of diurnal cycles.</p>
<p>Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with nitrate (NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected DIC concentration and pH of the growth media was determined for the different pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with constants from Mehrbach et al. (1973, refit by Dickson &amp; Millero 1987), and inputs of temperature, salinity, total alkalinity (2376.5 umol · kg-1), pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each phase of the experiments were manipulated by the addition of NaHCO3, and was then maintained by bubbling a CO2-Air mix through the cultures over the course of the experiments. The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml aliquots and each aliquot was inoculated with 5 ml of the T. pseudonana CCMP 1014 (TP1014) stock culture at the start of the experiments.</p>
<p>Macronutrient concentrations:<br />
Media was filtered through 0.2 um filters into clean (plastic) bottles and stored at -20 degrees-C until analyses for nutrients. During the experiment, subsamples were filtered through 0.2 micron filters for Chl-a analyses, and through GF/F filters for particulate carbon (POC) analyses. The filterate from these filtrations was pooled into acid-washed HDPE containers, and stored at -20 degrees-C until analyses.&nbsp; Phosphate (PO4), Nitrate (NO3) + Nitrite (NO2), and Silicic Acid (Si(OH)4) were measured by Flow injection analysis (FIA) using a QuikChem 8500 Series 2 AutoAnalyzer (Lachat Instruments, Zellweger Analytics, Inc.).</p>
<p>Nutrient detection limits are reported in the last record of the data table.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538602 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538602
completed
Uta Passow
University of California-Santa Barbara
709-864-8010
Ocean Sciences Centre, Memorial University Marine Lab Road, Logy Bay
St. John's
Newfoundland
A1C 5S7
Canada
uta.passow@mun.ca
pointOfContact
Edward Laws
Louisiana State University College of the Coast and Environment
225 578-3334
Louisiana State University School of the Coast and Environment 1002R Energy, Coast and Environment Building
Baton Rouge
LA
70803
USA
edlaws@lsu.edu
pointOfContact
Nigel D'Souza
University of California-Santa Barbara
419-819-9039
Marine Science Institute
nigel.dsouza@lifesci.ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Phase
CO2
Temperature
Day
Replicate
SOL_PO4
OL_PO4
EL_PO4
SOL_SiO4
OL_SiO4
EL_SiO4
SOL_NO3_NO2
OL_NO3_NO2
EL_NO3_NO2
Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic)
•QuikChem 8500 Series 2 AutoAnalyzer (Lachat Instruments, Zellweger Analytics, Inc.)
theme
None, User defined
sample description
Partial pressure of CO2
water temperature at measurement depth
days
replicate
reactive phosphorus (PO4)
Silicate, Si(OH)4, silicic acid
nitrate plus nitrite
featureType
BCO-DMO Standard Parameters
Cell Cultivator
Nutrient Autoanalyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
https://osprey.bco-dmo.org/project/654347
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
<p>The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.</p>
<p>Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.</p>
Stressors on Marine Phytoplankton
largerWorkCitation
project
eng; USA
oceans
2018-07-01
2018-10-31
0
BCO-DMO catalogue of parameters from Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Phosphate, silicate, and nitrate plus nitrite measurements
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/771384.rdf
Name: Phase
Units: unitless
Description: <p>Indicates whether the sample was collected during the acclimation phase or the experiment phase of the experiment. The last record gives the instrument detection limits. Note that some concentrations in some treatments were below detection limits.</p>
http://lod.bco-dmo.org/id/dataset-parameter/771385.rdf
Name: CO2
Units: parts per million (ppm)
Description: <p>Indicates the concentration of CO2 in the CO2-Air mix that was bubbled through the samples over the course of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/771386.rdf
Name: Temperature
Units: degrees Celsius
Description: <p>Indicates the temperature at which the samples were incubated.</p>
http://lod.bco-dmo.org/id/dataset-parameter/771387.rdf
Name: Day
Units: unitless
Description: <p>Indicates the timepoint (day) of sampling. D0 = day 0; D1 = day 1; etc.</p>
http://lod.bco-dmo.org/id/dataset-parameter/771388.rdf
Name: Replicate
Units: unitless
Description: <p>Indicates replication within a treatment. "NA" indicates "not applicable"</p>
http://lod.bco-dmo.org/id/dataset-parameter/771389.rdf
Name: SOL_PO4
Units: microMol
Description: <p>Phosphate concentrations in samples incubated at sub optimum light (SOL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/771390.rdf
Name: OL_PO4
Units: microMol
Description: <p>Phosphate concentrations in samples incubated at optimum light (OL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/771391.rdf
Name: EL_PO4
Units: microMol
Description: <p>Phosphate concentrations in samples incubated at extreme light (EL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/771392.rdf
Name: SOL_SiO4
Units: microMol
Description: <p>Silicate concentrations in samples incubated at sub optimum light (SOL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/771393.rdf
Name: OL_SiO4
Units: microMol
Description: <p>Silicate concentrations in samples incubated at optimum light (OL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/771394.rdf
Name: EL_SiO4
Units: microMol
Description: <p>Silicate concentrations in samples incubated at extreme light (EL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/771395.rdf
Name: SOL_NO3_NO2
Units: microMol
Description: <p>Nitrate + Nitrite concentrations in samples incubated at sub optimum light (SOL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/771396.rdf
Name: OL_NO3_NO2
Units: microMol
Description: <p>Nitrate + Nitrite concentrations in samples incubated at optimum light (OL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/771397.rdf
Name: EL_NO3_NO2
Units: microMol
Description: <p>Nitrate + Nitrite concentrations in samples incubated at extreme light (E</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7072
https://darchive.mblwhoilibrary.org/bitstream/1912/25909/1/dataset-771370_series-3a-nutrients__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.771370.1
download
onLine
dataset
<p>Three CO2 concentrations were tested: 410 ppm, 750 ppm, and 1000 ppm respectively. For each CO2 concentration, four temperatures were tested: 15 degrees-C, 20 degrees-C, 25 degrees-C, and 30 degrees-C. Within each temperature, three light levels were tested: a sub-optimum light (SOL) intensity of 60 umol photons · m-2 · s-1, an optimum light (OL) intensity of 400 umol photons · m-2 · s-1 and an extreme light (EL) intensity of 800 umol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series, with all light treatments at two temperatures (either 15 degrees-C and 25 degrees-C or 20 degrees-C and 30 degrees-C) running simultaneously. This was repeated for each CO2 concentration.</p>
<p>Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2um filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile artificial seawater, and the humidified gas mix was supplied to each tube via gentle sparging through a 2um stainless steel diffuser. Flow rates were gradually increased over the course of the incubation to compensate for the DIC uptake of actively growing cells, and ranged from &lt;0.04 Liters per minute (LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days. For each CO2 and temperature level, replication was achieved by incubating three tubes at sub-optimum light intensities, two tubes at optimum light intensity, and three tubes at extreme light intensities. Each experiment was split into two phases: An acclimation phase spanning 4 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 3-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 5 hours into the daily light cycle to minimize the effects of diurnal cycles.</p>
<p>Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with nitrate (NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected DIC concentration and pH of the growth media was determined for the different pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with constants from Mehrbach et al. (1973, refit by Dickson &amp; Millero 1987), and inputs of temperature, salinity, total alkalinity (2376.5 umol · kg-1), pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each phase of the experiments were manipulated by the addition of NaHCO3, and was then maintained by bubbling a CO2-Air mix through the cultures over the course of the experiments. The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml aliquots and each aliquot was inoculated with 5 ml of the T. pseudonana CCMP 1014 (TP1014) stock culture at the start of the experiments.</p>
<p>Macronutrient concentrations:<br />
Media was filtered through 0.2 um filters into clean (plastic) bottles and stored at -20 degrees-C until analyses for nutrients. During the experiment, subsamples were filtered through 0.2 micron filters for Chl-a analyses, and through GF/F filters for particulate carbon (POC) analyses. The filterate from these filtrations was pooled into acid-washed HDPE containers, and stored at -20 degrees-C until analyses.&nbsp; Phosphate (PO4), Nitrate (NO3) + Nitrite (NO2), and Silicic Acid (Si(OH)4) were measured by Flow injection analysis (FIA) using a QuikChem 8500 Series 2 AutoAnalyzer (Lachat Instruments, Zellweger Analytics, Inc.).</p>
<p>Nutrient detection limits are reported in the last record of the data table.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- changed "- NA -" to "NA" ("not applicable")</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic)
Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic)
PI Supplied Instrument Name: Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic) PI Supplied Instrument Description:Used for incubation of TP1014 cultures. Instrument Name: Cell Cultivator Instrument Short Name: Instrument Description: An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.
•QuikChem 8500 Series 2 AutoAnalyzer (Lachat Instruments, Zellweger Analytics, Inc.)
•QuikChem 8500 Series 2 AutoAnalyzer (Lachat Instruments, Zellweger Analytics, Inc.)
PI Supplied Instrument Name: •QuikChem 8500 Series 2 AutoAnalyzer (Lachat Instruments, Zellweger Analytics, Inc.) PI Supplied Instrument Description:Used for analysis of nutrient (N, P, Si) concentrations. Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/