http://lod.bco-dmo.org/id/dataset/771701
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-06-24
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Bulk and cell-specific CO2 fixation and PO4 uptake from Atlantic Explorer cruise AE1524 (BATS validation cruise BV50), September 2015
2019-06-24
publication
2019-06-24
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-08-19
publication
https://doi.org/10.1575/1912/bco-dmo.771701.1
Solange Duhamel
Lamont-Doherty Earth Observatory
principalInvestigator
Dr O. Roger Anderson
Lamont-Doherty Earth Observatory
principalInvestigator
Eunsoo Kim
American Museum of Natural History
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Duhamel, S., Anderson, O., Kim, E. (2019) Bulk and cell-specific CO2 fixation and PO4 uptake from Atlantic Explorer cruise AE1524 (BATS validation cruise BV50), September 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-06-24 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.771701.1 [access date]
CO2 fixation and PO4 uptake rates Dataset Description: <p>Bulk and cell-specific CO2 fixation and PO4 uptake from Atlantic Explorer cruise AE1524 (BATS validation cruise BV50), September 2015 in the North Atlantic subtropical gyre (September 2015), along a transect from Bermuda to Puerto Rico extending from 33°N to 22°N. Phosphate uptake rates were measured in Prochlorococcus, Synechococcus, pigmented eukaryotes, and unpigmented eukaryotes. Also reported are CO2 fixation rate by Prochlorococcus, Synechococcus, and pigmented eukaryotes. These data were published by Duhamel et al (2019), Table 3.</p> Methods and Sampling: <p>Seawater was collected into acid washed, ultra-pure water and sample rinsed, clear polycarbonate incubation bottles. PO4 assimilation rates were measured in triplicate 70-mL samples with ~259 kBq of added 33P-PO4 (Perkin-Elmer #NEZ08000; carrier free), incubated for 30 min to 1h. CO2 fixation rates were measured in duplicate 70-mL samples with ~17 MBq of added 14C-sodium bicarbonate (Perkin Elmer #NEC086H000, 1.6 GBq/mmol), incubated from dawn to dusk. Samples were incubated under simulated light and temperature conditions measured at the sampling site. A killed control sample was also prepared by adding paraformaldehyde (PFA, 2 % final concentration prepared with electron microscopy grade 16 % aqueous solution, Electron Microscopy Sciences) at least 15 minutes before introducing the radioisotope, in order to account for unincorporated radioactivity. At the end of incubation, samples were fixed with PFA (2% final, for 15-min in the dark), and triplicate 20-microliters aliquots were sampled to measure the total radioactivity added (with beta-phenylethylamine for 14C samples). The total microbial activity was determined by filtering a 3-mL aliquot through a 0.2-micron, pore-size polycarbonate membrane filter (Nuclepore). To reduce unincorporated 33P-PO4, the membrane filter was placed onto a filter type HA soaked in 100 mM KH2PO4, then rinsed three times with ~1 mL of 0.2-micron filtered seawater. To remove unincorporated 14C-sodium bicarbonate, the filter was acidified with 0.5 mL of 1N HCl for 24 h. To determine plankton groups specific uptake rates, a 20-mL aliquot was passed through a 0.2-micron polycarbonate membrane filter under gentle vacuum filtration, and the remaining volume from the 70-mL incubation bottle was passed through a 0.8-micron polycarbonate membrane filter. The 0.2-micron and 0.8-micron filters were stored in separate cryovials with 2 mL and 4 mL of the corresponding radiolabeled sample, respectively, vortexed to detach the cells from the filter, then flash frozen for later flow cytometric sorting (see below). The added radioactivity and total microbial activity were assayed by liquid scintillation counting in 7-mL plastic scintillation vials (Simport) with 4 mL of scintillation cocktail (Ultima Gold LLT, Perkin Elmer) added.</p>
<p>Turnover times (TPO4, h) were calculated by dividing the total radioactivity added (Bq L–1) by the rate of radiolabel uptake into the particulate fraction (Bq L–1 h–1). PO4 assimilation rates (nmol P L–1 h–1) were calculated by dividing PO4 concentration by TPO4. We used PO4 concentration estimated from a concentration series bioassays following the method of Wright and Hobbie (1966). Briefly, seawater samples were amended with non-radioactive PO4 to target additions of 0, 5, 10, 25, 50, 75, and 150 nmol PO4 L–1, spiked with 33P-PO4, incubated and sampled as described above. The resulting TPO4 values were plotted against a corresponding concentration of PO4, and extrapolated using linear regression (TPO4 = a x PO4 + b) to estimate the ambient concentration (Sn = b/a), which represents an upper estimate of ambient concentrations as detailed in Zubkov and Tarran (2005). Results from these bioassays were also used to calculate the Michaelis-Menten kinetic parameters for PO4 assimilation rates (Vmax, the maximum rate at saturating substrate concentration and Km, the half-saturation constant).</p>
<p>For cell sorting of Prochlorococcus, Synechococcus, pigmented and non-pigmented protists, the Influx flow cytometer was set at the highest sorting purity (1.0 drop single mode) and potential attached cells were discarded using a pulse width vs. forward scatter plot. The drop delay was calibrated using Accudrop Beads (BD Biosciences, USA) and verified manually by sorting a specified number of reference beads onto a glass slide and counting the beads under an epifluorescence microscope. Performance was validated as described in Duhamel et al. (2018). Three to four proportional numbers of cells from the same incubation sample were sorted for each target population. Sorted cells were assessed by liquid scintillation analysis following previously published protocols (Talarmin et al. 2011; Duhamel et al. 2012; Rii et al. 2016). The 14C-labeled samples were acidified with 1 mL of 2 mol L-1 HCl for 24 h to remove any unincorporated 14C-sodium bicarbonate.</p>
<p>For each group, at least three samples were sorted and regression analysis between the number of cells sorted and the radioactivity taken up by the sorted cells was used to calculate the per cell activity (dpm cell−1). Radioactivity in sorted cells from the PFA-killed control samples (dpm cell−1) was deduced from radioactivity in the sorted cells from the respective samples (dpm cell−1). The cell-specific assimilation rate (nmol cell-1 h-1) was calculated by dividing the radioactivity per cell (dpm cell−1) by the total microbial activity (dpm L−1) measured in the same sample, and then multiplied by the total microbial assimilation rate at ambient substrate concentration (nmol L−1 h−1).</p>
<p>Michaelis– Menten kinetic parameters were determined using the Michaelis–Menten model in Prism 6.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1458070 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1458070
completed
Solange Duhamel
Lamont-Doherty Earth Observatory
520-621-6047
1007 E Lowell Street Life Sciences South 315
Tucson
AZ
85721
USA
duhamel@arizona.edu
pointOfContact
Dr O. Roger Anderson
Lamont-Doherty Earth Observatory
845-365-8452
Biology and Paleoenvironment Division 61 Route 9W - Marine Biology
Palisades
NY
10964-8000
USA
ora@ldeo.columbia.edu
pointOfContact
Eunsoo Kim
American Museum of Natural History
212-769-5975
Central Park West at 79th St
New York
NY
10024-5192
USA
ekim1@amnh.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
cruise
station
lat
lon
PO4
PO4_33P
Vmax
Km
CO2_14C
PO4_Pro_33P
PO4_Syn_33P
PO4_P_Euk_33P
PO4_NP_Euk_33P
CO2_Pro_14C
CO2_Syn_14C
CO2_P_Euk_14C
BD Influx flow cytometer
Epifluorescence microscope
Horiba FluoroMax-4 spectrofluorometer
Packard Tri-Carb 3110 TR liquid scintillation counter with ultra-low-level option kit
theme
None, User defined
Cruise identfier
station
latitude
longitude
reactive phosphorus (PO4)
radiotracer phosphate uptake
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Flow Cytometer
Fluorescence Microscope
Fluorometer
Liquid Scintillation Counter
instrument
BCO-DMO Standard Instruments
AE1524
service
Deployment Activity
North Atlantic subtropical gyre
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Role of small-sized protists in the microbial loop with emphasis on interactions between mixotrophic protists and picocyanobacteria
https://osprey.bco-dmo.org/project/542308
Collaborative Research: Role of small-sized protists in the microbial loop with emphasis on interactions between mixotrophic protists and picocyanobacteria
<p>This project is an NSF Collaborative Research Project.</p>
<p><em>Description from NSF award abstract:</em><br />
Protists are mostly single-celled, eukaryotic microorganisms, including algae and protozoans. They are ubiquitous, diverse, and major contributors in oceanic food webs. Determining their taxonomic identity and the extent to which they contribute to carbon and nutrient cycles (whereby carbon and minerals are continuously changed chemically in the environment and reincorporated in living organisms) are among the major goals of this study. Moreover, the investigators will study how they respond to environmental change, one of the most important and challenging current problems in oceanography. Answering these questions is fundamental to understanding how living organisms in the ocean environment interact with one another and contribute to the health and productivity of the ocean. The main goal of the project is to investigate biotic interactions of small-sized protists with very tiny cyanobacteria also known as picocyanobacteria, which represent the most abundant photosynthetic organisms in the ocean. These studies will be done both in ocean environments and in laboratory experimental settings. Considering the limited knowledge on this topic, the work planned in this project promises important and exciting discoveries. Two early career female scientists will lead this project. In addition, one postdoctoral scholar, one graduate student, and at least three undergraduate summer interns will participate in the proposed research activities. The principal investigators will create a strong public outreach program that will engage middle school students in hands-on activities related to ocean sciences, and will produce a video in collaboration with the Education Department at the American Museum of Natural History. The video will summarize the major findings of the proposed research. It can be used in schools and in informal learning settings, including access by the public on the Internet through the Museum's Science Bulletins web page.</p>
<p>Single-celled eukaryotic microorganisms or protists, though largely outnumbered by picocyanobacteria (<em>Prochlorococcus</em> and <em>Synechococcus</em>), contribute significantly to ocean carbon biomass and primary productivity, partially by virtue of their larger cell size. In addition, small planktonic protists can regulate picocyanobacteria abundance through grazing. The main goal of this project is to investigate biotic interactions of planktonic pico- and nano-sized eukaryotes with picocyanobacteria, both in the field and in laboratory settings. A set of field- and culture-based experiments will be conducted, using state-of-the-art methodologies, including fluorescence-activated cell sorting, isotope and fluorescent stain labeling, and next-generation molecular sequencing to address the research objectives.</p>
<p>Operationally, this project is structured around two objectives:</p>
<p>Objective 1 is to assess the contribution of small protists to carbon and nutrient cycling through measurement of primary production, bacterivory, mixotrophy and phosphorus uptake in major microbial groups, and evaluate the role of nutrient availability in controlling mixotrophy.</p>
<p>Objective 2 will focus on assessing the distribution and diversity of small-sized protists that feed on picocyanobacteria and further evaluate the role of nutrient availability among the protists that are mixotrophic.</p>
<p>To reach these objectives field-based experiments will be conducted in contrasted environments: the North Pacific subtropical gyre (phosphorus replete, dominated by <em>Prochlorococcus</em> at Sta. ALOHA) and the North West Mediterranean sea (phosphorus deplete, dominated by <em>Synechococcu</em>s at Sta. DYFAMED). Complementary experiments using model protists and picocyanobacteria will be conducted using controlled cultures in the lab. The work will provide critical new information on the phylogenetic diversity and function of marine microbial eukaryotes, with emphasis on their ecological role as predators (phagotrophy, mixotrophy) on, and competitors with, the picoyanobacteria<em> Prochlorococcus</em> and <em>Synechococcus</em>.</p>
Small protists in microbial loop
largerWorkCitation
project
eng; USA
oceans
North Atlantic subtropical gyre
-65.37
-64.14
22.16
33.25
2015-09-14
2015-09-21
North Pacific subtropical gyre (Station ALOHA) and Northwestern Mediterranean Sea (Station DYFAMED)
0
BCO-DMO catalogue of parameters from Bulk and cell-specific CO2 fixation and PO4 uptake from Atlantic Explorer cruise AE1524 (BATS validation cruise BV50), September 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/775678.rdf
Name: cruise
Units: unitless
Description: <p>cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/775679.rdf
Name: station
Units: unitless
Description: <p>station identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/775680.rdf
Name: lat
Units: decimal degrees
Description: <p>station latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/775681.rdf
Name: lon
Units: decimal degrees
Description: <p>station longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/775682.rdf
Name: PO4
Units: nanomol Phosphate/liter (nmol P L-1)
Description: <p>Phosphate concentration estimate</p>
http://lod.bco-dmo.org/id/dataset-parameter/775683.rdf
Name: PO4_33P
Units: nanomol Phosphate/liter/hour (nmol P L-1 h-1)
Description: <p>Bulk phosphate uptake rate (>0.2 microns) </p>
http://lod.bco-dmo.org/id/dataset-parameter/775684.rdf
Name: Vmax
Units: nanomol Phosphate/liter/hour (nmol P L-1 h-1)
Description: <p>Phosphate uptake kinetic parameter Vmax</p>
http://lod.bco-dmo.org/id/dataset-parameter/775685.rdf
Name: Km
Units: nanomol Phosphate/liter (nmol P L-1)
Description: <p>Phosphate uptake kinetic parameter Km</p>
http://lod.bco-dmo.org/id/dataset-parameter/775686.rdf
Name: CO2_14C
Units: milligrams Carbon/meter^3/day (mg C m-3 d-1)
Description: <p>Bulk CO2 fixation rate (>0.2 microns)</p>
http://lod.bco-dmo.org/id/dataset-parameter/775687.rdf
Name: PO4_Pro_33P
Units: attomole/cell/hour (amol cell-1 h-1)
Description: <p>Phosphate uptake rate by Prochlorococcus</p>
http://lod.bco-dmo.org/id/dataset-parameter/775688.rdf
Name: PO4_Syn_33P
Units: attomole/cell/hour (amol cell-1 h-1)
Description: <p>Phosphate uptake rate by Synechococcus</p>
http://lod.bco-dmo.org/id/dataset-parameter/775689.rdf
Name: PO4_P_Euk_33P
Units: attomole/cell/hour (amol cell-1 h-1)
Description: <p>Phosphate uptake rate by pigmented eukaryotes</p>
http://lod.bco-dmo.org/id/dataset-parameter/775690.rdf
Name: PO4_NP_Euk_33P
Units: attomole/cell/hour (amol cell-1 h-1)
Description: <p>Phosphate uptake rate by non-pigmented eukaryotes</p>
http://lod.bco-dmo.org/id/dataset-parameter/775691.rdf
Name: CO2_Pro_14C
Units: femtogram Carbon/cell/hour (fg C cell-1 h-1)
Description: <p>CO2 fixation rate by Prochlorococcus</p>
http://lod.bco-dmo.org/id/dataset-parameter/775692.rdf
Name: CO2_Syn_14C
Units: femtogram Carbon/cell/hour (fg C cell-1 h-1)
Description: <p>CO2 fixation rate by Synechococcus</p>
http://lod.bco-dmo.org/id/dataset-parameter/775693.rdf
Name: CO2_P_Euk_14C
Units: femtogram Carbon/cell/hour (fg C cell-1 h-1)
Description: <p>CO2 fixation rate by pigmented eukaryotes</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
508
https://darchive.mblwhoilibrary.org/bitstream/1912/24457/1/dataset-771701_rv-atlantic-explorer-bats-bv50-co2-and-po4__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.771701.1
download
onLine
dataset
<p>Seawater was collected into acid washed, ultra-pure water and sample rinsed, clear polycarbonate incubation bottles. PO4 assimilation rates were measured in triplicate 70-mL samples with ~259 kBq of added 33P-PO4 (Perkin-Elmer #NEZ08000; carrier free), incubated for 30 min to 1h. CO2 fixation rates were measured in duplicate 70-mL samples with ~17 MBq of added 14C-sodium bicarbonate (Perkin Elmer #NEC086H000, 1.6 GBq/mmol), incubated from dawn to dusk. Samples were incubated under simulated light and temperature conditions measured at the sampling site. A killed control sample was also prepared by adding paraformaldehyde (PFA, 2 % final concentration prepared with electron microscopy grade 16 % aqueous solution, Electron Microscopy Sciences) at least 15 minutes before introducing the radioisotope, in order to account for unincorporated radioactivity. At the end of incubation, samples were fixed with PFA (2% final, for 15-min in the dark), and triplicate 20-microliters aliquots were sampled to measure the total radioactivity added (with beta-phenylethylamine for 14C samples). The total microbial activity was determined by filtering a 3-mL aliquot through a 0.2-micron, pore-size polycarbonate membrane filter (Nuclepore). To reduce unincorporated 33P-PO4, the membrane filter was placed onto a filter type HA soaked in 100 mM KH2PO4, then rinsed three times with ~1 mL of 0.2-micron filtered seawater. To remove unincorporated 14C-sodium bicarbonate, the filter was acidified with 0.5 mL of 1N HCl for 24 h. To determine plankton groups specific uptake rates, a 20-mL aliquot was passed through a 0.2-micron polycarbonate membrane filter under gentle vacuum filtration, and the remaining volume from the 70-mL incubation bottle was passed through a 0.8-micron polycarbonate membrane filter. The 0.2-micron and 0.8-micron filters were stored in separate cryovials with 2 mL and 4 mL of the corresponding radiolabeled sample, respectively, vortexed to detach the cells from the filter, then flash frozen for later flow cytometric sorting (see below). The added radioactivity and total microbial activity were assayed by liquid scintillation counting in 7-mL plastic scintillation vials (Simport) with 4 mL of scintillation cocktail (Ultima Gold LLT, Perkin Elmer) added.</p>
<p>Turnover times (TPO4, h) were calculated by dividing the total radioactivity added (Bq L–1) by the rate of radiolabel uptake into the particulate fraction (Bq L–1 h–1). PO4 assimilation rates (nmol P L–1 h–1) were calculated by dividing PO4 concentration by TPO4. We used PO4 concentration estimated from a concentration series bioassays following the method of Wright and Hobbie (1966). Briefly, seawater samples were amended with non-radioactive PO4 to target additions of 0, 5, 10, 25, 50, 75, and 150 nmol PO4 L–1, spiked with 33P-PO4, incubated and sampled as described above. The resulting TPO4 values were plotted against a corresponding concentration of PO4, and extrapolated using linear regression (TPO4 = a x PO4 + b) to estimate the ambient concentration (Sn = b/a), which represents an upper estimate of ambient concentrations as detailed in Zubkov and Tarran (2005). Results from these bioassays were also used to calculate the Michaelis-Menten kinetic parameters for PO4 assimilation rates (Vmax, the maximum rate at saturating substrate concentration and Km, the half-saturation constant).</p>
<p>For cell sorting of Prochlorococcus, Synechococcus, pigmented and non-pigmented protists, the Influx flow cytometer was set at the highest sorting purity (1.0 drop single mode) and potential attached cells were discarded using a pulse width vs. forward scatter plot. The drop delay was calibrated using Accudrop Beads (BD Biosciences, USA) and verified manually by sorting a specified number of reference beads onto a glass slide and counting the beads under an epifluorescence microscope. Performance was validated as described in Duhamel et al. (2018). Three to four proportional numbers of cells from the same incubation sample were sorted for each target population. Sorted cells were assessed by liquid scintillation analysis following previously published protocols (Talarmin et al. 2011; Duhamel et al. 2012; Rii et al. 2016). The 14C-labeled samples were acidified with 1 mL of 2 mol L-1 HCl for 24 h to remove any unincorporated 14C-sodium bicarbonate.</p>
<p>For each group, at least three samples were sorted and regression analysis between the number of cells sorted and the radioactivity taken up by the sorted cells was used to calculate the per cell activity (dpm cell−1). Radioactivity in sorted cells from the PFA-killed control samples (dpm cell−1) was deduced from radioactivity in the sorted cells from the respective samples (dpm cell−1). The cell-specific assimilation rate (nmol cell-1 h-1) was calculated by dividing the radioactivity per cell (dpm cell−1) by the total microbial activity (dpm L−1) measured in the same sample, and then multiplied by the total microbial assimilation rate at ambient substrate concentration (nmol L−1 h−1).</p>
<p>Michaelis– Menten kinetic parameters were determined using the Michaelis–Menten model in Prism 6.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- added cruise column</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
BD Influx flow cytometer
BD Influx flow cytometer
PI Supplied Instrument Name: BD Influx flow cytometer PI Supplied Instrument Description:Used for flow cytometry analyses Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Epifluorescence microscope
Epifluorescence microscope
PI Supplied Instrument Name: Epifluorescence microscope PI Supplied Instrument Description:Used to count calibration beads. Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
Horiba FluoroMax-4 spectrofluorometer
Horiba FluoroMax-4 spectrofluorometer
PI Supplied Instrument Name: Horiba FluoroMax-4 spectrofluorometer PI Supplied Instrument Description:Used to measure fluorescence Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Packard Tri-Carb 3110 TR liquid scintillation counter with ultra-low-level option kit
Packard Tri-Carb 3110 TR liquid scintillation counter with ultra-low-level option kit
PI Supplied Instrument Name: Packard Tri-Carb 3110 TR liquid scintillation counter with ultra-low-level option kit PI Supplied Instrument Description:Used to assay sample radioactivity. Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
Cruise: AE1524
AE1524
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel