http://lod.bco-dmo.org/id/dataset/771777
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-06-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Quantification of different chemical forms of cobalamin from San Pedro Ocean Time Series (SPOT) cruises on R/V Nerissa and R/V Yellowfin from March to December 2017
2019-06-26
publication
2019-06-26
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-01-04
publication
https://doi.org/10.26008/1912/bco-dmo.771777.1
Sergio A. Sanudo-Wilhelmy
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sanudo-Wilhelmy, S. A. (2021) Quantification of different chemical forms of cobalamin from San Pedro Ocean Time Series (SPOT) cruises on R/V Nerissa and R/V Yellowfin from March to December 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-06-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.771777.1 [access date]
Quantification of different chemical forms of cobalamin from SPOT cruises in 2017 Dataset Description: Methods and Sampling: <p>Samples for quantification of the different cobalamin forms were collected at a six of depths within the euphotic zone (5-250m). Seawater was collected from each CTD depth using Niskin bottles and immediately filtered. Cobalamins were analyzed as in 1 Suffridge et al. (2017). Two liters of seawater were filtered through µm pore-size filters and then preconcentrated using a C18 resin (HF Bondesil (Agilent Technologies) and analyzed by liquid chromatography/triple mass spectrometry (LC/MS/MS/MS). The LC-MS system consists of a ThermoTSQ Quantum Access electro-spray ionization triple quadrupole mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography system. The LC system used a stable- bond C18 reversed-phase column (DiscoveryHSC18 10cm × 2.1mm,5 μm column, Supelco Analytical) with a100 μL sample loop.In order to increase the sensitivity and precision,theLC/MS was run in full-loop mode (100 μl/injection).</p>
<p>The LC-MS system used for the pigment quantification consists of a Thermo TSQ Quantum Access electro-spray ionization triple quadruple mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography pump and auto-sampler.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559276 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559276
completed
Sergio A. Sanudo-Wilhelmy
University of Southern California
213-821-1302
3616 Trousdale Pkwy Dept. of Biological Sciences, AHF 206, Mail Code: 0371
Los Angeles
CA
90089
USA
sanudo@usc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
Date
Time_Start
Time_End
Longitude
Latitude
Depth
AB12
HB12
MB12
CB12
MET
ThermoTSQ Quantum Access
Niskin bottles
theme
None, User defined
Cruise identfier
date
time of day
longitude
latitude
depth
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Mass Spectrometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
SPOT_Nerissa_Cruises_2017
SPOT_Yellowfin_Cruises
service
Deployment Activity
San Pedro Ocean Time Series
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The role of organic and metal cofactors on the biogenic synthesis of halogenated volatile hydrocarbons
https://osprey.bco-dmo.org/project/712782
The role of organic and metal cofactors on the biogenic synthesis of halogenated volatile hydrocarbons
<p><em>NSF Award Abstract:</em><br />
Volatile halogenated hydrocarbon gases, in this case halomethanes, are produced naturally by organisms in the ocean; which then serves as a source of these biogenic gases to the atmosphere. Their chemical reactions in the atmosphere are very similar to those of anthropogenic chlorofluorocarbons (CFCs). While CFCs are well-studied because they consume the ozone in the upper atmosphere that shields the earth from harmful ultraviolet radiation, halomethanes have been largely neglected, even though they currently account for 25% of the ozone depletion. As anthropogenic CFC levels steadily decline, however, halomethanes are predicted to account for 50% of ozone depletion by 2050. Based on limited study thus far, marine halomethane production has been ascribed mainly to phytoplankton and macro algae. This project will build on new and compelling data that suggests marine heterotrophic bacteria could also be major producers of halomethanes. The data produced here will provide the critical evaluation required to address discrepancies in global halomethane budgets which currently are out of balance due to an unknown source to the atmosphere, evaluating the hypothesis that marine heterotrophic bacteria can supply this missing source. Concerns over the stability of the earth's stratospheric ozone layer make this valuable and necessary research with added value of providing support for engaged undergraduate, graduate, and postdoctoral education at the University of Southern California.</p>
<p>Past research on the production of marine halomethanes has focused on phytoplankton and macro algae, while potential bacterial contributions to the processe have been neglected. This research proposes to study the role of marine heterotrophic bacteria on the production of halomethanes. It has been noted in past studies that there are discrepancies in the global atmospheric halomethane budget, and it is possible this is due to a large missing bacterial source. Additionally, this research will evaluate the potential importance of vitamin B12, methionine, and vanadium cofactors on the synthesis of halomethanes in bacteria. A large portion of marine bacteria cannot synthesize methylation co-enzymes, and therefore, would require available B12, methionine, and vanadium from external sources to complete the methylation step. This study will also measure concentrations of halomethanes, B12, methionine, and vanadium in upwelling regions as well as at a long-term time series site in order to put constraints on the variability of halomethanes concentrations for use in global linked air-sea models.</p>
Volatile_Hydrocarbons
largerWorkCitation
project
eng; USA
oceans
San Pedro Ocean Time Series
-118.4
-118.4
33.55
33.55
2017-03-15
2017-12-15
0
BCO-DMO catalogue of parameters from Quantification of different chemical forms of cobalamin from San Pedro Ocean Time Series (SPOT) cruises on R/V Nerissa and R/V Yellowfin from March to December 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/771878.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise name and date</p>
http://lod.bco-dmo.org/id/dataset-parameter/771879.rdf
Name: Date
Units: unitless
Description: <p>Date; format: yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/771880.rdf
Name: Time_Start
Units: unitless
Description: <p>Start time; format: HH:MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/771881.rdf
Name: Time_End
Units: unitless
Description: <p>End time; format: HH:MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/771882.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/771883.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/771884.rdf
Name: Depth
Units: meters (m)
Description: <p>Sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/771885.rdf
Name: AB12
Units: picomoles per liter (pM)
Description: <p>Average Adenosyl-B12 (Adenosylcobalamin) concentrations of triplicate analytical replicates using HPLC-MS.</p>
http://lod.bco-dmo.org/id/dataset-parameter/771886.rdf
Name: HB12
Units: picomoles per liter (pM)
Description: <p>Average Hydroxy-B12 (Hydroxycobalamin) concentrations of triplicate analytical replicates using HPLC-MS.</p>
http://lod.bco-dmo.org/id/dataset-parameter/771887.rdf
Name: MB12
Units: picomoles per liter (pM)
Description: <p>Average Methyl-B12 (Methylcobalamin) concentrations of triplicate analytical replicates using HPLC-MS.</p>
http://lod.bco-dmo.org/id/dataset-parameter/771888.rdf
Name: CB12
Units: picomoles per liter (pM)
Description: <p>Average Vitamin B12 (as Cyanocobalamin) concentrations of triplicate analytical replicates using HPLC-MS.</p>
http://lod.bco-dmo.org/id/dataset-parameter/771889.rdf
Name: MET
Units: picomoles per liter (pM)
Description: <p>Average Methionine concentrations of triplicate analytical replicates using HPLC-MS.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3522
https://darchive.mblwhoilibrary.org/bitstream/1912/26526/1/dataset-771777_spot-cobalamins__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.771777.1
download
onLine
dataset
<p>Samples for quantification of the different cobalamin forms were collected at a six of depths within the euphotic zone (5-250m). Seawater was collected from each CTD depth using Niskin bottles and immediately filtered. Cobalamins were analyzed as in 1 Suffridge et al. (2017). Two liters of seawater were filtered through µm pore-size filters and then preconcentrated using a C18 resin (HF Bondesil (Agilent Technologies) and analyzed by liquid chromatography/triple mass spectrometry (LC/MS/MS/MS). The LC-MS system consists of a ThermoTSQ Quantum Access electro-spray ionization triple quadrupole mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography system. The LC system used a stable- bond C18 reversed-phase column (DiscoveryHSC18 10cm × 2.1mm,5 μm column, Supelco Analytical) with a100 μL sample loop.In order to increase the sensitivity and precision,theLC/MS was run in full-loop mode (100 μl/injection).</p>
<p>The LC-MS system used for the pigment quantification consists of a Thermo TSQ Quantum Access electro-spray ionization triple quadruple mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography pump and auto-sampler.</p>
Specified by the Principal Investigator(s)
<p>The LC-MS data was processed using Xcalibur and LCQUAN quantitative softwares from Thermo Scientific.</p>
<p>BCO-DMO Processing:<br />
- modified parameter names (removed units, renamed date and time columns);<br />
- re-formatted date to yyyy-mm-dd (was m/dd/yyyy);<br />
- split time column into start and end times;<br />
- converted lat and lon from degrees and minutes to decimal degrees.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
ThermoTSQ Quantum Access
ThermoTSQ Quantum Access
PI Supplied Instrument Name: ThermoTSQ Quantum Access PI Supplied Instrument Description:The LC-MS system used for the pigment quantification consists of a Thermo TSQ Quantum Access electro-spray ionization triple quadruple mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography pump and auto-sampler. Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: SPOT_Nerissa_Cruises_2017
SPOT_Nerissa_Cruises_2017
R/V Nerissa
vessel
Cruise: SPOT_Yellowfin_Cruises
SPOT_Yellowfin_Cruises
R/V Yellowfin
R/V Yellowfin
vessel
R/V Nerissa
vessel
R/V Yellowfin
R/V Yellowfin
vessel