http://lod.bco-dmo.org/id/dataset/773129
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-07-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Porewater nutrient concentrations (NO3+NO2, NH4, and PO4) from pushcore samples collected at Guaymas Basin hydrothermal vents via Alvin dives on RV/Atlantis cruise AT42-05, Nov. 2018 and reported by Woods Hole Oceanographic Institution
2019-07-15
publication
2019-07-15
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-07-31
publication
https://doi.org/10.1575/1912/bco-dmo.773129.1
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
principalInvestigator
Andreas Teske
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Edgcomb, V., Teske, A. (2019) Porewater nutrient concentrations (NO3+NO2, NH4, and PO4) from pushcore samples collected at Guaymas Basin hydrothermal vents via Alvin dives on RV/Atlantis cruise AT42-05, Nov. 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-07-15 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.773129.1 [access date]
Guaymas pushcore nutrients (WHOI) Dataset Description: <p>Porewater nutrient concentrations from pushcore samples collected at two locations in the Guaymas Basin hydrothermal vents region during Alvin dives on RV/Atlantis cruise AT42-05, Nov. 2018. NO3+NO2, NH4, and PO4 concentrations at different depths within the cores are reported. These samples are reported by the PI at&nbsp;Woods Hole Oceanographic Institution.</p>
<p>Nutrient porewater samples from this cruise were also analyzed at the Max Planck Institute for Marine Microbiology. See related dataset: <a href="https://www.bco-dmo.org/dataset/816549" target="_blank">https://www.bco-dmo.org/dataset/816549</a> (MPI)</p> Methods and Sampling: <p>Alvin pushcores dedicated to nutrient analyses were sectioned to recover the 0-6cm, 6-12, and 12-18cm fractions, or the 0-10, 10-20, and 20-30cm fractions. Two ~40ml samples of sediment were placed in 50 ml Falcon tubes and were centrifuged at 3000 rpm for 15 minutes to separate porewater from the sediment. After centrifugation, the porewater was filtered through sterile syringe cellulose 0.45 micron filters. 5 ml of the porewater was placed into 5 ml screwcap Eppendorf cryotubes stored without headspace at -20 degrees C for nitrate (NO3), nitrite (NO2) and phosphate (PO4) analysis. The headspace was flushed with nitrogen and the samples were stored inverted at 4 degrees C.</p>
<p>All porewater analyses were performed at Louisiana State University Wetland Biogeochemistry Analytical Services. Dilution info: All samples @ 2x and various samples @ 20x , 50x , 70x , and/or 350x.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829903 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829903
completed
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
508-289-3734
Department of Geology and Geophysics 220 McLean Lab, MS #8
Woods Hole
MA
02543
USA
vedgcomb@whoi.edu
pointOfContact
Andreas Teske
University of North Carolina at Chapel Hill
508-287-1648
Department of Marine Sciences 3117B Venable Hall, CB 3300
Chapel Hill
NC
27599
USA
teske@email.unc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date_local
Site
Dive
Sample_ID
NO3_NO2_uM
NH4_uM
PO4_uM
lat
lon
theme
None, User defined
date_local
site
dive_id
sample identification
nitrate plus nitrite
Ammonium
reactive phosphorus (PO4)
latitude
longitude
featureType
BCO-DMO Standard Parameters
Nutrient Autoanalyzer
Push Corer
instrument
BCO-DMO Standard Instruments
AT42-05
AT42-05_Alvin_Dives
service
Deployment Activity
Guaymas Basin, Gulf of California, Mexico
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Hydrothermal Fungi in the Guaymas Basin Hydrocarbon Ecosystem
https://osprey.bco-dmo.org/project/770633
Collaborative Research: Hydrothermal Fungi in the Guaymas Basin Hydrocarbon Ecosystem
<p>NSF Award Abstract:<br />
Fungi that can derive energy from chemicals, yet consume other organisms or organic material to obtain carbon have been reported from diverse marine subsurface samples, including from hundreds of meters below the seafloor. Evidence exists that Fungi are active in subsurface marine sediments globally, yet there is a dearth of knowledge on their role in the marine subsurface, and specifically on their role(s) in hydrocarbon degradation within deep-sea sediments. This team is isolating a broad collection of environmentally relevant filamentous Fungi and yeasts from hydrothermally-influenced and hydrocarbon-rich seep sediments of Guaymas Basin using high-throughput culture-based approaches. They aim to reveal the diversity of Fungi and Bacteria in these hydrothermal sediments, how temperature and hydrocarbon composition shape their distribution, and how Fungi cooperate to enhance the degradation of hydrocarbons by Bacteria. By hosting six undergraduates through the WHOI Summer Student Fellows program and the Woods Hole Partnership Education Program, the project contributes to increasing diversity in marine science by offering opportunities for promising undergraduates from disadvantaged populations. High school students are involved in summer projects and in intensive summer workshops. One postdoc, a graduate student, and two Research Associates are supported, and international collaborations are strengthened. The postdoc and graduate student are gaining valuable cruise-based experience. An e-lecture on Fungi and their role(s) in biodegradation of hydrocarbons will be made publicly available by the end of the project. Fungal isolates with accompanying information will be secured in a reference culture collection for long-term storage and are available to any interested researcher throughout the project.</p>
<p>The PIs are isolating a broad collection of environmentally relevant filamentous Fungi and yeasts from hydrothermally-influenced and hydrocarbon-rich seep sediments of Guaymas Basin using high-throughput culture-based approaches, with the aim to reveal their ability to degrade individual hydrocarbons under in situ pressures and temperatures. Culture independent methods marker gene analyses are used to characterize in situ fungal and bacterial diversity and to examine how temperature and hydrocarbon composition shape fungal community composition and distribution. Traditional and comprehensive two-dimensional gas chromatographic analyses are used to examine the complexities and subtle changes in inventories of hydrocarbons within sediment cores, and provide evidence for in situ microbial alteration of individual hydrocarbons. Incubation experiments are used to test the ability of fungal isolates to utilize different hydrocarbons as a sole or auxiliary carbon source under in situ pressures and temperatures and their ability to stimulate biodegradation of hydrocarbons by hydrocarbon-degrading bacteria. Expressed genes within these incubation studies tell us how Fungi and Bacteria couple metabolisms to increase overall specificity and extent of biodegradation of hydrocarbons.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
HOTFUN
largerWorkCitation
project
eng; USA
oceans
Guaymas Basin, Gulf of California, Mexico
-111.4071
-111.4044
27.0078
27.0116
2018-11-17
2018-11-25
Guaymas Basin, Gulf of CA, Mexico
0
BCO-DMO catalogue of parameters from Porewater nutrient concentrations (NO3+NO2, NH4, and PO4) from pushcore samples collected at Guaymas Basin hydrothermal vents via Alvin dives on RV/Atlantis cruise AT42-05, Nov. 2018 and reported by Woods Hole Oceanographic Institution
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/773386.rdf
Name: Date_local
Units: unitless
Description: <p>sampling date - local time (UTC-07:00)</p>
http://lod.bco-dmo.org/id/dataset-parameter/773387.rdf
Name: Site
Units: unitless
Description: <p>sampling site name</p>
http://lod.bco-dmo.org/id/dataset-parameter/773388.rdf
Name: Dive
Units: unitless
Description: <p>Alvin dive number</p>
http://lod.bco-dmo.org/id/dataset-parameter/773389.rdf
Name: Sample_ID
Units: unitless
Description: <p>sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/773442.rdf
Name: NO3_NO2_uM
Units: micromoles/liter (uM)
Description: <p>nitrates and nitrites concentration from the push core pore water</p>
http://lod.bco-dmo.org/id/dataset-parameter/773443.rdf
Name: NH4_uM
Units: micromoles/liter (uM)
Description: <p>ammonium concentration from the push core pore water</p>
http://lod.bco-dmo.org/id/dataset-parameter/773444.rdf
Name: PO4_uM
Units: micromoles/liter (uM)
Description: <p>phosphates concentration from the push core pore water</p>
http://lod.bco-dmo.org/id/dataset-parameter/773866.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/773867.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3920
https://darchive.mblwhoilibrary.org/bitstream/1912/24372/1/dataset-773129_hydrothermal-vent-porewater-nutrients-no3no2-nh4-and-po4__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.773129.1
download
onLine
dataset
<p>Alvin pushcores dedicated to nutrient analyses were sectioned to recover the 0-6cm, 6-12, and 12-18cm fractions, or the 0-10, 10-20, and 20-30cm fractions. Two ~40ml samples of sediment were placed in 50 ml Falcon tubes and were centrifuged at 3000 rpm for 15 minutes to separate porewater from the sediment. After centrifugation, the porewater was filtered through sterile syringe cellulose 0.45 micron filters. 5 ml of the porewater was placed into 5 ml screwcap Eppendorf cryotubes stored without headspace at -20 degrees C for nitrate (NO3), nitrite (NO2) and phosphate (PO4) analysis. The headspace was flushed with nitrogen and the samples were stored inverted at 4 degrees C.</p>
<p>All porewater analyses were performed at Louisiana State University Wetland Biogeochemistry Analytical Services. Dilution info: All samples @ 2x and various samples @ 20x , 50x , 70x , and/or 350x.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- re-formatted date from d/m/yyyy to yyyy-mm-dd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
PI Supplied Instrument Name: PI Supplied Instrument Description:Used to collect sediment samples Instrument Name: Push Corer Instrument Short Name: Instrument Description: Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.
Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/
Cruise: AT42-05
AT42-05
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT42-05
Andreas Teske
University of North Carolina at Chapel Hill
Deployment: AT42-05_Alvin_Dives
AT42-05_Alvin_Dives
HOV Alvin
Community Standard Description
International Council for the Exploration of the Sea
HOV Alvin
submarine-manned
AT42-05_Alvin_Dives
Andreas Teske
University of North Carolina at Chapel Hill
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
HOV Alvin
Community Standard Description
International Council for the Exploration of the Sea
HOV Alvin
submarine-manned