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Coastal marine ecosystems are seasonally dynamic and highly productive. Phytoplankton populations shift from nutrient replete conditions in the spring to nutrient poor conditions in other seasons. The San Pedro Ocean Time-series (SPOT), located 17 km offshore between Los Angeles Harbor and Catalina Island, is a representative and accessible model coastal system with regular sampling and a substantial archive of relevant observations. The SPOT program has cataloged the dynamics, diversity, and productivity of microbial populations since 2000. With rising carbon dioxide (CO2) concentrations and resulting decreases in surface pH, it is critically important to understand the nutrient controls on primary production in coastal waters and the capacity of coastal ecosystems to sequester CO2. This project will examine rates of primary production, nitrogen uptake associated with primary production, and the oxidation of ammonium to nitrate (nitrification), at SPOT over two seasonal cycles. It will also contribute to the development of human resources in the marine sciences through the training of undergraduate and graduate students at the University of Southern California and the University of Maryland. The researchers participate in education outreach activities (e.g. through the Centers for Ocean Sciences Education Excellence programs), and will incorporate findings from this study in those presentations.&lt;/p&gt;
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&lt;p&gt;2. Forms of dissolved organic N, and specifically urea, can be substrates for nitrification and contribute substantially to regenerated production.&lt;/p&gt;
&lt;p&gt;See the related project &quot;&lt;a href=&quot;http://www.bco-dmo.org/project/560420&quot; target=&quot;_blank&quot;&gt;Direct Identification and Characterization of Marine Heterotrophic Nitrogen Fixers by Stable Isotope Probing&lt;/a&gt;&quot;, funded by OCE-1341178, that involved novel stable isotope probing (SIP) methods.&lt;/p&gt;</gco:CharacterString>
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