http://lod.bco-dmo.org/id/dataset/774017
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-08-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
PUA (polyunsaturated aldehydes) experiments: Chlorophyll-a concentrations, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015
2019-07-31
publication
2019-07-31
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-10-09
publication
https://doi.org/10.1575/1912/bco-dmo.774017.1
Dr Peter Lavrentyev
University of Akron
principalInvestigator
James J. Pierson
University of Maryland Center for Environmental Science
principalInvestigator
Diane Stoecker
University of Maryland Center for Environmental Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lavrentyev, P., Pierson, J., Stoecker, D. (2019) PUA (polyunsaturated aldehydes) experiments: Chlorophyll-a concentrations, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-07-31 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.774017.1 [access date]
PUA chl-a Dataset Description: <p>This dataset reports the chlorophyll-a concentrations for polyunsaturated aldehydes (PUA) experiments with samples from Virginia Coastal Bays and Bay of Napoli.</p> Methods and Sampling: <p>Experiments were conducted by collecting raw seawater, filtering it through 200µm mesh sieves into 20L carboys, and then dispensing it into experimental jars. Triplicates bottles were used for each treatment. Treatments included whole seawater (control), whole seawater plus copepods (Zooplankton), and the same treatments plus PUA additions (Heptadienal, Octadienal, Decadienal, and Mixed PUA). PUA were dissolved in methanol and added to experimental bottles for a final concentration of 21 nM; for the mixed PUA treatment this was 7nM of each type of PUA.</p>
<p>Initial samples were collected from the carboy for chlorophyll a as described below. Final samples were collected from each treatment and control bottle as described below.</p>
<p>Chlorophyll a samples (50-150ml) were filtered onto 25mm diameter GF/F filters (nominal 0.7 micrometer&nbsp;pore size), and filters were extracted in 90% acetone at -20 degrees C for 24 hours using the acid method (EPA Method 445 - Arar &amp; Collins 1997), then analyzed on a Turner Designs AU10 or TD700 fluorometer.</p>
<p>All data were processed in Microsoft Excel.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357168 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357168
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357169 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357169
completed
Dr Peter Lavrentyev
University of Akron
330-972-7922
Department of Biology 302 Buchtel Common
Akron
OH
44325-3908
USA
peter3@uakron.edu
pointOfContact
James J. Pierson
University of Maryland Center for Environmental Science
410-221-8218
Horn Point Laboratory 2020 Horns Point Road
Cambridge
MD
21613
USA
jpierson@umces.edu
pointOfContact
Diane Stoecker
University of Maryland Center for Environmental Science
410-221-8407
UMCES, Horn Point Lab P.O. Box 775
Cambridge
MD
21613
USA
stoecker@umces.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Experiment
Treatment
Rep
Chl
Turner Designs AU10 fluorometer
theme
None, User defined
experiment id
treatment
replicate
chlorophyll a
featureType
BCO-DMO Standard Parameters
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters
https://osprey.bco-dmo.org/project/555556
The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters
<p><em>Description from NSF award abstract:</em><br />
This project will conduct a set of field/laboratory experiments to address the following hypotheses with respect to microzooplankton (consumers between 20-200 um) and diatom- produced polyunsaturated aldehydes:<br />
I. Aldehydes will impair microzooplankton herbivory on diatoms and non-diatom phytoplankton.<br />
II. Aldehydes will reduce the growth rates of microzooplankton and non PUA-producing phytoplankton.<br />
III. In the presence of aldehyde-producing diatoms, copepods will switch to microzooplankton, whereas non- (mildly)- toxic diatoms will be an important food source for copepods.<br />
IV. The effects of aldehydes on microzooplankton and copepods will depend on the grazers' prior exposure to PUA.</p>
<p>The experiments will include natural plankton, captured copepods, cultured Skeletonema marinoi (SM), including its aldehyde-producing strain, and synthetic aldehydes. To gain insights into complex interactions within planktonic communities, detailed information on their composition, abundance, and dynamics will be obtained using microscopy, flow-cytometry, and cytological methods. This approach will allow the PIs to draw conclusions about the role of diatom-produced aldehydes in phytoplankton-microzooplankton- copepod trophic interactions. The PIs will coordinate efforts and exchange information with the PUA study group at the Stazione Zoologica Anton Dohrn (Naples, Italy).</p>
<p>Diatoms are dominant autotrophic plankton in the ocean. Recent evidence indicates that microzooplankton are the dominant herbivores, whereas copepods often rely on microzooplankton as food, except during peak diatom production. The ability of microzooplankton to feed on large diatoms and grow as fast as their algal prey leads to the question of what allows diatoms to escape microzooplankton grazing control during the initial phases of their blooms and maintain the blooms until nutrient resources are depleted? Allelopathy is wide spread among phytoplankton. The cosmopolitan bloom-forming SM produces several aldehydes and has become a model organism in plankton allelopathy studies. Most studies on diatom cytotoxicity have been dedicated to inhibitory effects on reproduction and development of marine invertebrates, whereas surprisingly little information exists on its impact on key diatom grazers, microzooplankton. Preliminary results in the Chesapeake Bay show that aldehydes may induce cascading effects within planktonic communities. The proposed study will: (1) Improve our knowledge of the critical diatom-microzooplankton-copepod links in the coastal ocean; (2) Generate novel data on the effects of allelopathy on marine food webs; (3) Contribute to our understanding of broader patterns of marine ecosystems by comparing plankton structure and dynamics in the temperate Atlantic waters; (4) Advance biological oceanography through international collaboration.</p>
DiatomAldehydes
largerWorkCitation
project
eng; USA
biota
oceans
-75.9866
14.25
37.1656
40.808
2015-03-23
2015-07-02
0
BCO-DMO catalogue of parameters from PUA (polyunsaturated aldehydes) experiments: Chlorophyll-a concentrations, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/774029.rdf
Name: Experiment
Units: unitless
Description: <p>Name of the Experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/774030.rdf
Name: Treatment
Units: unitless
Description: <p>Treatment name</p>
http://lod.bco-dmo.org/id/dataset-parameter/774031.rdf
Name: Rep
Units: unitless
Description: <p>Replicate number</p>
http://lod.bco-dmo.org/id/dataset-parameter/774032.rdf
Name: Chl
Units: micrograms/liter
Description: <p>Chlorophyll a concentration determined from acetone extraction</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3369
https://darchive.mblwhoilibrary.org/bitstream/1912/24682/1/dataset-774017_pua-chlorophyll__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.774017.1
download
onLine
dataset
<p>Experiments were conducted by collecting raw seawater, filtering it through 200µm mesh sieves into 20L carboys, and then dispensing it into experimental jars. Triplicates bottles were used for each treatment. Treatments included whole seawater (control), whole seawater plus copepods (Zooplankton), and the same treatments plus PUA additions (Heptadienal, Octadienal, Decadienal, and Mixed PUA). PUA were dissolved in methanol and added to experimental bottles for a final concentration of 21 nM; for the mixed PUA treatment this was 7nM of each type of PUA.</p>
<p>Initial samples were collected from the carboy for chlorophyll a as described below. Final samples were collected from each treatment and control bottle as described below.</p>
<p>Chlorophyll a samples (50-150ml) were filtered onto 25mm diameter GF/F filters (nominal 0.7 micrometer&nbsp;pore size), and filters were extracted in 90% acetone at -20 degrees C for 24 hours using the acid method (EPA Method 445 - Arar &amp; Collins 1997), then analyzed on a Turner Designs AU10 or TD700 fluorometer.</p>
<p>All data were processed in Microsoft Excel.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- reduced some Chl_a values to 6 decimal places</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Turner Designs AU10 fluorometer
Turner Designs AU10 fluorometer
PI Supplied Instrument Name: Turner Designs AU10 fluorometer PI Supplied Instrument Description:Chlorophyll a concentrations were determined on a Turner Designs AU10 fluorometer after extraction in 90% acetone at 0°C. The fluorometer was calibrated using a 5 point calibration with a chlorophyll a standard Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/