http://lod.bco-dmo.org/id/dataset/774033
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-08-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Microzooplankton biomass estimates from PUA (polyunsaturated aldehydes) experiments, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015
2019-09-30
publication
2019-09-30
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-10-09
publication
https://doi.org/10.1575/1912/bco-dmo.774033.1
Dr Peter Lavrentyev
University of Akron
principalInvestigator
James J. Pierson
University of Maryland Center for Environmental Science
principalInvestigator
Diane Stoecker
University of Maryland Center for Environmental Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lavrentyev, P., Pierson, J., Stoecker, D. (2019) Microzooplankton biomass estimates from PUA (polyunsaturated aldehydes) experiments, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-09-30 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.774033.1 [access date]
PUA Microzooplankton Biomass Dataset Description: <p>This dataset reports the microzooplankton biomass&nbsp;for polyunsaturated aldehydes (PUA) experiments. Samples were from Virginia coastal bays and the Bay of Napoli, and were conducted between March and July, 2015.</p> Methods and Sampling: <p>Experiments were conducted by collecting raw seawater, filtering it through 200µm mesh sieves into 20L carboys, and then dispensing it into experimental jars. Triplicates bottles were used for each treatment. Treatments included whole seawater (control), whole seawater plus copepods (Zooplankton), and the same treatments plus PUA additions (Heptadienal, Octadienal, Decadienal, and Mixed PUA). PUA were dissolved in methanol and added to experimental bottles for a final concentration of 21 nM; for the mixed PUA treatment this was 7nM of each type of PUA.</p>
<p>Initial samples were collected from the carboy for microzooplankton as described below. Final samples were collected from each treatment and control bottle as described below.</p>
<p>Microzooplankton samples were collected by gently decanting 100ml of each treatment bottle into a sample bottle and preserved with 2% acid Lugol’s solution (final concentration). Identification and sorting of microzooplankton was done by settling 10-25 ml of sample in Utermöhl chambers and counting with an Olympus IX-70 inverted microscope equipped with differential interference contrast (DIC), epifluorescence, and a digital camera. Microzooplankton biovolumes were calculated from their dimensions and approximate shapes (Sun and Liu 2003), and converted to carbon using published empirical relationships (Menden-Deuer and Lessard 2000). Tintinnid volumes were calculated based on their cell dimensions.</p>
<p>All data were processed in Microsoft Excel.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357168 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357168
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357169 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357169
completed
Dr Peter Lavrentyev
University of Akron
330-972-7922
Department of Biology 302 Buchtel Common
Akron
OH
44325-3908
USA
peter3@uakron.edu
pointOfContact
James J. Pierson
University of Maryland Center for Environmental Science
410-221-8218
Horn Point Laboratory 2020 Horns Point Road
Cambridge
MD
21613
USA
jpierson@umces.edu
pointOfContact
Diane Stoecker
University of Maryland Center for Environmental Science
410-221-8407
UMCES, Horn Point Lab P.O. Box 775
Cambridge
MD
21613
USA
stoecker@umces.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Experiment
Bottle_ID
Treatment
Rep
Taxa
Phylum
ESD
Biomass
Olympus IX-70 inverted microscope
theme
None, User defined
experiment id
sample identification
treatment
replicate
taxon
phylum
cell_size
wet_weight_biomass
featureType
BCO-DMO Standard Parameters
Inverted Microscope
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters
https://osprey.bco-dmo.org/project/555556
The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters
<p><em>Description from NSF award abstract:</em><br />
This project will conduct a set of field/laboratory experiments to address the following hypotheses with respect to microzooplankton (consumers between 20-200 um) and diatom- produced polyunsaturated aldehydes:<br />
I. Aldehydes will impair microzooplankton herbivory on diatoms and non-diatom phytoplankton.<br />
II. Aldehydes will reduce the growth rates of microzooplankton and non PUA-producing phytoplankton.<br />
III. In the presence of aldehyde-producing diatoms, copepods will switch to microzooplankton, whereas non- (mildly)- toxic diatoms will be an important food source for copepods.<br />
IV. The effects of aldehydes on microzooplankton and copepods will depend on the grazers' prior exposure to PUA.</p>
<p>The experiments will include natural plankton, captured copepods, cultured Skeletonema marinoi (SM), including its aldehyde-producing strain, and synthetic aldehydes. To gain insights into complex interactions within planktonic communities, detailed information on their composition, abundance, and dynamics will be obtained using microscopy, flow-cytometry, and cytological methods. This approach will allow the PIs to draw conclusions about the role of diatom-produced aldehydes in phytoplankton-microzooplankton- copepod trophic interactions. The PIs will coordinate efforts and exchange information with the PUA study group at the Stazione Zoologica Anton Dohrn (Naples, Italy).</p>
<p>Diatoms are dominant autotrophic plankton in the ocean. Recent evidence indicates that microzooplankton are the dominant herbivores, whereas copepods often rely on microzooplankton as food, except during peak diatom production. The ability of microzooplankton to feed on large diatoms and grow as fast as their algal prey leads to the question of what allows diatoms to escape microzooplankton grazing control during the initial phases of their blooms and maintain the blooms until nutrient resources are depleted? Allelopathy is wide spread among phytoplankton. The cosmopolitan bloom-forming SM produces several aldehydes and has become a model organism in plankton allelopathy studies. Most studies on diatom cytotoxicity have been dedicated to inhibitory effects on reproduction and development of marine invertebrates, whereas surprisingly little information exists on its impact on key diatom grazers, microzooplankton. Preliminary results in the Chesapeake Bay show that aldehydes may induce cascading effects within planktonic communities. The proposed study will: (1) Improve our knowledge of the critical diatom-microzooplankton-copepod links in the coastal ocean; (2) Generate novel data on the effects of allelopathy on marine food webs; (3) Contribute to our understanding of broader patterns of marine ecosystems by comparing plankton structure and dynamics in the temperate Atlantic waters; (4) Advance biological oceanography through international collaboration.</p>
DiatomAldehydes
largerWorkCitation
project
eng; USA
biota
oceans
-75.9866
14.25
37.1656
40.808
2015-03-23
2015-07-02
0
BCO-DMO catalogue of parameters from Microzooplankton biomass estimates from PUA (polyunsaturated aldehydes) experiments, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/774050.rdf
Name: Experiment
Units: unitless
Description: <p>Name of the Experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/774051.rdf
Name: Bottle_ID
Units: unitless
Description: <p>ID name for a given treatment including both treatment and replicate tags</p>
http://lod.bco-dmo.org/id/dataset-parameter/774052.rdf
Name: Treatment
Units: unitless
Description: <p>Treatment name</p>
http://lod.bco-dmo.org/id/dataset-parameter/774053.rdf
Name: Rep
Units: unitless
Description: <p>Replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/774054.rdf
Name: Taxa
Units: unitless
Description: <p>Taxonomic name of microzooplankton type identified</p>
http://lod.bco-dmo.org/id/dataset-parameter/774055.rdf
Name: Phylum
Units: unitless
Description: <p>Phylum name for the taxa identified</p>
http://lod.bco-dmo.org/id/dataset-parameter/774056.rdf
Name: ESD
Units: micrometers
Description: <p>Mean size of the taxa; given as equivalent spherical diameter (ESD)</p>
http://lod.bco-dmo.org/id/dataset-parameter/774057.rdf
Name: Biomass
Units: micrograms carbon/liter
Description: <p>Biomass of the given taxa</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
149525
https://darchive.mblwhoilibrary.org/bitstream/1912/24681/1/dataset-774033_pua-microzooplankton-biomass__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.774033.1
download
onLine
dataset
<p>Experiments were conducted by collecting raw seawater, filtering it through 200µm mesh sieves into 20L carboys, and then dispensing it into experimental jars. Triplicates bottles were used for each treatment. Treatments included whole seawater (control), whole seawater plus copepods (Zooplankton), and the same treatments plus PUA additions (Heptadienal, Octadienal, Decadienal, and Mixed PUA). PUA were dissolved in methanol and added to experimental bottles for a final concentration of 21 nM; for the mixed PUA treatment this was 7nM of each type of PUA.</p>
<p>Initial samples were collected from the carboy for microzooplankton as described below. Final samples were collected from each treatment and control bottle as described below.</p>
<p>Microzooplankton samples were collected by gently decanting 100ml of each treatment bottle into a sample bottle and preserved with 2% acid Lugol’s solution (final concentration). Identification and sorting of microzooplankton was done by settling 10-25 ml of sample in Utermöhl chambers and counting with an Olympus IX-70 inverted microscope equipped with differential interference contrast (DIC), epifluorescence, and a digital camera. Microzooplankton biovolumes were calculated from their dimensions and approximate shapes (Sun and Liu 2003), and converted to carbon using published empirical relationships (Menden-Deuer and Lessard 2000). Tintinnid volumes were calculated based on their cell dimensions.</p>
<p>All data were processed in Microsoft Excel.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:<br />
-&nbsp;</strong>added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- reduced some Chla values from 15 to 4 decimal places<br />
- replaced blank cells with 'nd' (no data)<br />
- corrected taxon and phylum names according to WoRMS (World Registry of Marine Species) taxa matching tool</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Olympus IX-70 inverted microscope
Olympus IX-70 inverted microscope
PI Supplied Instrument Name: Olympus IX-70 inverted microscope PI Supplied Instrument Description:The microscope was equipped with differential interference contrast (DIC), epifluorescence, and a digital camera. Biomass estimates for microzooplankton were determined after counting cells on an inverted microscope and converting volume estimates, from measurements with a reticle in the objective lens, to carbon concentrations using known conversion factors. Instrument Name: Inverted Microscope Instrument Short Name: Instrument Description: An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).
Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives.
The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/