http://lod.bco-dmo.org/id/dataset/774288
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-08-05
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Extracted chlorophyll data, particulate carbon and nitrogen, flow cytometry and 15N2/13C rate measurements collected during EN596 from April 2017 in the North Atlantic
2019-08-05
publication
2019-08-05
revision
BCO-DMO Linked Data URI
2019-08-05
creation
http://lod.bco-dmo.org/id/dataset/774288
Angelicque E. White
Oregon State University
principalInvestigator
Julie Granger
University of Connecticut
principalInvestigator
Pia Moisander
University of Massachusetts Dartmouth
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: White, A. E., Moisander, P., Granger, J. (2019) Extracted chlorophyll data, particulate carbon and nitrogen, flow cytometry and 15N2/13C rate measurements collected during EN596 from April 2017 in the North Atlantic. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2019-08-05 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/774288 [access date]
Discrete samples and rate measurements collected aboard the RV Endeavor cruise EN596 Dataset Description: Methods and Sampling: <p><strong>15N Nitrogen Fixation, 13C Primary Production:</strong><br />
Standard protocols were used to measure in vitro primary productivity via the 13C radiotracer method [Lengendre et al., 1996] and N2-fixation rate measurements via a modified bubble release 15N2 method modified from Montoya et al [1996]. Briefly, water samples were collected before dawn in 4.3L polycarbonate bottles, a plug of 4ml of 15N2 gas was injected through a septa cap using a gas tight syringe. Bottles were then inverted for 15 minutes using a gas sample tumbler to assist in bringing the gas into solution. Following mixing the bubble was released and a Tinitial was collected for 15N2 enrichment. Bottles were then topped off using water for the same depth, sealed with caps fitted with septum and injected with 0.5ml of 47mM 13C bicarbonate stock and gently mixed by inversion. Samples for 13C-DIC to test the 13C enrichment concentration were taken by removing 1ml of enriched sample volume and injecting it into an evacuated exetainer pre-loaded with 85% phosphoric acid. Bottles were incubated on deck in incubators screened to 48% of surface irradiance and plumbed with flow through surface seawater for temperature regulation. All bottles were incubated in the incubator for ~ 24hr. Duplicate light bottles were paired with dark bottles and time final bottles that received no enrichment. Time zero bottles were also collected and sacrificed for each station. Natural abundance of dissolved 15N2 samples were also collected at each station. Following dawn to dawn incubations, a sample for Tpost 15N2 enrichment was collected from each enriched bottle and the remaining volume was filtered onto a combusted 25mm glass fiber filter, filters were folded in half and placed in combusted aluminum foil and stored at -80C for later analysis. Post-cruise, all filters were thawed and dried overnight at 60C. Samples were then balled into Ag and Sn boats and plated for analysis. The carbon and nitrogen isotopic composition (13C and 15N, respectively) were analyzed by continuous-flow isotope ratio mass spectrometry using a Carlo Erba elemental analyzer (EA) connected to a Thermo DeltaPlus isotope ratio mass spectrometer (IRMS). For all samples the international standards USGS40 (d13C = -26.389 permil vs VPDB, d15N = -4.52 permil vs air) and USGS41 (d13C = +37.626permil vs VPDB, d15N = +47.6 permil vs air) were used to calibrate each run of samples and the international standard IAEA-600 (d13C = -27.771 permil vs VPDB, d15N = +1.0 permil vs air) was used as a check standard (i.e., not used to calibrate data).13C-DIC samples were sent to UC Davis for analysis, resultant data was used to correct the 13C DIC addition for each bottle.&nbsp;</p>
<p><strong>Diazotroph Abundance:</strong><br />
At select stations where N2 Fixation measurements were conducted, the entire volume of a Niskin bottle at 25m was gravity filtered through a 47-mm diameter, 10 micron pore size, black polycarbonate filter with a polyester drain disk as a backing filter. If the volume was not filtered after 2 hours, filtration was terminated and the remaining volume of the carboy was measured in order to calculate volume filtered.&nbsp; Following filtration, filter holders were fit with a short section of tubing and a syringe leur fitting on once side and a 2-way valve on the outflow side. For each filter, 5-ml of 2% glutaraldehyde was slowly injected onto the filter and samples were allowed to fix for 30 minutes. Fixative was drained after this time and 60ml of air was used to flush all filters. Polycarbonate filters were then mounted onto 3x2 glass slides with immersion oil, cover slides were added and the edges of each cover slip was sealed with quick dry nail polish. All slides were stored at -20C and counted within 30 days. Enumeration of diazotrophic taxa was performed using epifluorescence microscopy.The entire slide was counted for DDAs and Trichodesimum abundance. Endosymbiont bearing diatoms of the following genus were enumerated: Rhizosolenia, Hemiaulus, Climacodium and Chaetoceras. Free Richelia intracellularis were also counted. Trichodesmium filaments were counted and the length of each filament was recorded. Trichodesmium cell number was then calculated by dividing the filament length by the mean cell length (9.9 micron +/-2.5 micron).&nbsp;<br />
<br />
<strong>Flow Cytometry Bacteria:</strong><br />
Flow cytometry samples were collected from rosette nisken bottles at specific depths where 3ml of sample volume was pipette into cryovials, preserved by adding 60ul of 2% paraformaldehyde, allowed to sit in the dark for 10 minutes then stored in a -80C until analysis. Post cruise, the samples were thawed in the dark, a 250ul aliquot of sample was removed and added to 250ul Deionized water and 45ul 300mM Potassium citrate, 5ul of SYBR Green stain and 25ul of 1um beads were added, samples were run on a Becton Dickinson FACSCalibur flow cytometer using the methods adpated from Marie, et al 1997. All values are reported in cells/L, NaN are no data.<br />
<br />
<strong>Flow Cytometry-Phytoplankton:</strong><br />
Flow cytometry samples were collected from rosette nisken bottles at specific depths where 3ml of sample volume was pipette into cryovials, preserved by adding 60ul of 2% paraformaldehyde, allowed to sit in the dark for 10 minutes then stored in a -80C until analysis. Post cruise, the samples were thawed in the dark, a 500ul aliquot was removed and 3um beads were added post run, samples were run for 3mins on a Becton Dickinson FACSCalibur flow cytometer using the methods outlined in Sherr, et al 2002.&nbsp;<br />
<br />
<strong>Fluorometric Chlorophyll:</strong><br />
Fluorometric Chlorophyll were collected from rosette nisken bottles at specific depths and filtered at low vacuum through a glass fiber filter. Samples were then flash frozen in liquid nitrogen and stored in a histoprep capsule at -80C until analysis. Post cruise, the filters were extracted using 90% acetone for a total of 48 hours in a -20C freezer, covered to prevent the photodegradation of pigments. Upon extraction samples were centrifuged to settle filter particulates and gently poured into a glass cuvette. Samples were then run on a Turner 10-AU Fluorometer. Data was corrected for average glass fiber filter blank values and where duplicate samples were taken the average and standard deviation are reported. NaN are no data, duplicates not taken.<br />
<br />
<strong>Particulate Carbon (PC) and Particulate Nitrogen (PN):</strong><br />
The carbon, and nitrogen content of particulate material (PC, PN) was collected from rosette nisken bottles at specific depths and filtered at low vacuum through a combusted glass fiber filter (PCPN). Following filtration, filters for PCPN were folded in half and placed in combusted aluminum foil and stored at -80C for later analysis. Post-cruise, all filters were thawed, acidified for a minimum of 12 hours and dried overnight at 60C. Samples were then balled into Ag and Sn boats and plated for analysis. Organic carbon and total nitrogen content was analyzed using high-temperature combustion (1020 C) on a ThermoQuest NC 2500 elemental analyzer. Three standards with known %C and %N were weighed, folded in tin boats, and analyzed with the samples to develop the calibration equation for each run. The calibration equation was then used to calculate the mass (mg) of carbon and nitrogen in each sample using EAS Clarity Chromatography Station software. The standards are Cystine (Elemental Microanalysis, B2035, 11.66 %TN, 29.99 %TC), Atropine (Elemental Microanalysis, B2002, 4.84 %TN, 70.56%TC), and GoniLab Secondary Standard (low %C)-A1 (an internal standard of sediment from the Gulf of Mexico, MV0803, with low carbon - 0.12 %N, 1.3 %C).&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1732206 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1732206
onGoing
Angelicque E. White
Oregon State University
808-956-6220
1950 East West Rd.
Honolulu
HI
USA
aewhite@hawaii.edu
pointOfContact
Julie Granger
University of Connecticut
860-405-9094
Department of Marine Sciences 1080 Shenecossett Road
Groton
CT
06340
USA
julie.granger@uconn.edu
pointOfContact
Pia Moisander
University of Massachusetts Dartmouth
508-999-8222
Department of Biology 285 Old Westport Road
North Dartmouth
MA
02747
USA
pmoisander@umassd.edu
pointOfContact
asNeeded
Unknown
Julian_day
Station
Cast
Depth
Nisken
chl_avg
chl
chl_std
fix_rate_13C_avg
fix_rate_15N2_avg
fix_rate_13C_std_dev
fix_rate_15N2_std_dev
HNA
LNA
tot_bact
PC_avg
PC_std
PN_avg
PN_std
Picoeukaryotes
Prochlorococcus
Richella
SAL
SST
Synechococcus
tot_diaz
trico
cells_m3
Latitude
Longitude
Carlo Erba elemental analyzer (EA)
ThermoQuest NC 2500 elemental analyzer
epifluorescence microscopy
Thermo DeltaPlus isotope ratio mass spectrometer (IRMS)
nisken bottles
theme
None, User defined
Julian day as a decimal (time elapsed in days from 00:00 on January 1st 4713 BC)
station number
cast
depth
bottle
chlorophyll a
rate of 14C fixation
nitrogen fixation rate
bacterial abundance
total particulate Carbon
Total Particulate Nitrogen
pico_euks
prochlorococcus abundance
No BCO-DMO term
salinity
water temperature at measurement depth
synechococcus abundance
tricho
count
latitude
longitude
featureType
BCO-DMO Standard Parameters
Carlo-Erba NA-1500 Elemental Analyzer
Elemental Analyzer
Fluorescence Microscope
Isotope-ratio Mass Spectrometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
EN596
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean
https://osprey.bco-dmo.org/project/772534
EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean
<p>NSF Award Abstract:<br />
The availability of nitrogen is required to support the growth and production of organisms living in the surface of our global ocean. This element can be scarce. To alleviate this scarcity, a special class of bacteria and archaea, called nitrogen fixers, can derive the nitrogen needed for growth from nitrogen gas. This project would carefully examine one specific method for measuring nitrogen fixation that has been used recently to suggest the occurrence of small amounts of nitrogen fixation in subsurface ocean waters. If these reports are verified, then a revision of our understanding of the marine nitrogen cycle may be needed. The Ocean Carbon and Biogeochemistry program will be used as a platform to develop community consensus for best practices in nitrogen fixation measurements and detection of diversity, activity, and abundances of the organisms responsible. In addition, a session will be organized in a future national/international conference to communicate with the broader scientific community while developing these best practices.</p>
<p>The goal of this study is to conduct a thorough examination of potential experimental and analytical errors inherent to the 15N2-tracer nitrogen fixation method, in tandem with comprehensive molecular measurements, in mesopelagic ocean waters. Samples will be collected and experimental work conducted on a cruise transect in the North Atlantic Ocean, followed by analytical work in the laboratory. The specific aims of this study are to (1) determine the minimum quantifiable rates of 15N2 fixation based on incubations of mesopelagic waters via characterization of sources of experimental and analytical error, and (2) seek evidence of presence and expression of nitrogen fixation genes via comprehensive molecular approaches on corresponding samples. The range of detectable rates and diazotroph activity from the measurements made in this study will be informative for the understanding of the importance of nitrogen fixation in the oceanic nitrogen budget.</p>
EAGER NitFix
largerWorkCitation
project
eng; USA
oceans
2019-08-05
North Atlantic Ocean, Pacific Ocean
0
BCO-DMO catalogue of parameters from Extracted chlorophyll data, particulate carbon and nitrogen, flow cytometry and 15N2/13C rate measurements collected during EN596 from April 2017 in the North Atlantic
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/774424.rdf
Name: Julian_day
Units: unitless
Description: <p>Julian day of station and cast</p>
http://lod.bco-dmo.org/id/dataset-parameter/774425.rdf
Name: Station
Units: unitless
Description: <p>station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/774426.rdf
Name: Cast
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/774427.rdf
Name: Depth
Units: meters
Description: <p>sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/774428.rdf
Name: Nisken
Units: unitless
Description: <p>nisken bottle number</p>
http://lod.bco-dmo.org/id/dataset-parameter/774429.rdf
Name: chl_avg
Units: micrograms per liter (ug/L)
Description: <p>average chlorophyll concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/774430.rdf
Name: chl
Units: micrograms per liter (ug/L)
Description: <p>chlorophyll concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/774431.rdf
Name: chl_std
Units: micrograms per liter (ug/L)
Description: <p>standard deviation of chlorophyll concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/774432.rdf
Name: fix_rate_13C_avg
Units: milligrams Carbon per cubic meter per day (mg C/m3/day)
Description: <p>Average 13C Fixation Rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/774433.rdf
Name: fix_rate_15N2_avg
Units: nanomole per liter per day (nmol/L/day)
Description: <p>Average 15N Fixation Rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/774434.rdf
Name: fix_rate_13C_std_dev
Units: milligrams Carbon per cubic meter per day (mg C/m3/day)
Description: <p>standard deviation of 13C Fixation Rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/774435.rdf
Name: fix_rate_15N2_std_dev
Units: nanomole per liter per day (nmol/L/day)
Description: <p>standard deviation of 15N Fixation Rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/774436.rdf
Name: HNA
Units: cells per liter (cells/L)
Description: <p>High Nucleic Acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/774437.rdf
Name: LNA
Units: cells per liter (cells/L)
Description: <p>Low Nucleic Acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/774438.rdf
Name: tot_bact
Units: cells per liter (cells/L)
Description: <p>sum of High Nucleic Acid and Low Nucleic Acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/774439.rdf
Name: PC_avg
Units: micromole Carbon (umol C)
Description: <p>Particulate Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/774440.rdf
Name: PC_std
Units: micromole Carbon (umol C)
Description: <p>Particulate carbon standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/774441.rdf
Name: PN_avg
Units: N/L
Description: <p>Particulate Nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/774442.rdf
Name: PN_std
Units: N/L
Description: <p>Particulate Nitrogen standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/774443.rdf
Name: Picoeukaryotes
Units: cells per liter (cells/L)
Description: <p>Picoeukaryotes</p>
http://lod.bco-dmo.org/id/dataset-parameter/774444.rdf
Name: Prochlorococcus
Units: cells per liter (cells/L)
Description: <p>Prochlorococcus</p>
http://lod.bco-dmo.org/id/dataset-parameter/774445.rdf
Name: Richella
Units: heterocysts per liter (heterocysts/L)
Description: <p>Free Richella intracellularis</p>
http://lod.bco-dmo.org/id/dataset-parameter/774446.rdf
Name: SAL
Units: psu
Description: <p>salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/774447.rdf
Name: SST
Units: degrees Celsius
Description: <p>sea surface temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/774448.rdf
Name: Synechococcus
Units: cells per liter (cells/L)
Description: <p>Synechococcus</p>
http://lod.bco-dmo.org/id/dataset-parameter/774449.rdf
Name: tot_diaz
Units: cells per liter (cells/L)
Description: <p>abundance of large diazotrophs</p>
http://lod.bco-dmo.org/id/dataset-parameter/774450.rdf
Name: trico
Units: cells per liter (cells/L)
Description: <p>Trichodesmium cell counts</p>
http://lod.bco-dmo.org/id/dataset-parameter/774451.rdf
Name: cells_m3
Units: cells per meter cubed (clls/m3)
Description: <p>total cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/774452.rdf
Name: Latitude
Units: decimal degrees
Description: <p>latitude in decimal degrees. Positive incated north.</p>
http://lod.bco-dmo.org/id/dataset-parameter/774453.rdf
Name: Longitude
Units: decimal_degrees
Description: <p>longitude in decimal degrees. Positive indicates East.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/774288/data/download
download
onLine
dataset
<p><strong>15N Nitrogen Fixation, 13C Primary Production:</strong><br />
Standard protocols were used to measure in vitro primary productivity via the 13C radiotracer method [Lengendre et al., 1996] and N2-fixation rate measurements via a modified bubble release 15N2 method modified from Montoya et al [1996]. Briefly, water samples were collected before dawn in 4.3L polycarbonate bottles, a plug of 4ml of 15N2 gas was injected through a septa cap using a gas tight syringe. Bottles were then inverted for 15 minutes using a gas sample tumbler to assist in bringing the gas into solution. Following mixing the bubble was released and a Tinitial was collected for 15N2 enrichment. Bottles were then topped off using water for the same depth, sealed with caps fitted with septum and injected with 0.5ml of 47mM 13C bicarbonate stock and gently mixed by inversion. Samples for 13C-DIC to test the 13C enrichment concentration were taken by removing 1ml of enriched sample volume and injecting it into an evacuated exetainer pre-loaded with 85% phosphoric acid. Bottles were incubated on deck in incubators screened to 48% of surface irradiance and plumbed with flow through surface seawater for temperature regulation. All bottles were incubated in the incubator for ~ 24hr. Duplicate light bottles were paired with dark bottles and time final bottles that received no enrichment. Time zero bottles were also collected and sacrificed for each station. Natural abundance of dissolved 15N2 samples were also collected at each station. Following dawn to dawn incubations, a sample for Tpost 15N2 enrichment was collected from each enriched bottle and the remaining volume was filtered onto a combusted 25mm glass fiber filter, filters were folded in half and placed in combusted aluminum foil and stored at -80C for later analysis. Post-cruise, all filters were thawed and dried overnight at 60C. Samples were then balled into Ag and Sn boats and plated for analysis. The carbon and nitrogen isotopic composition (13C and 15N, respectively) were analyzed by continuous-flow isotope ratio mass spectrometry using a Carlo Erba elemental analyzer (EA) connected to a Thermo DeltaPlus isotope ratio mass spectrometer (IRMS). For all samples the international standards USGS40 (d13C = -26.389 permil vs VPDB, d15N = -4.52 permil vs air) and USGS41 (d13C = +37.626permil vs VPDB, d15N = +47.6 permil vs air) were used to calibrate each run of samples and the international standard IAEA-600 (d13C = -27.771 permil vs VPDB, d15N = +1.0 permil vs air) was used as a check standard (i.e., not used to calibrate data).13C-DIC samples were sent to UC Davis for analysis, resultant data was used to correct the 13C DIC addition for each bottle.&nbsp;</p>
<p><strong>Diazotroph Abundance:</strong><br />
At select stations where N2 Fixation measurements were conducted, the entire volume of a Niskin bottle at 25m was gravity filtered through a 47-mm diameter, 10 micron pore size, black polycarbonate filter with a polyester drain disk as a backing filter. If the volume was not filtered after 2 hours, filtration was terminated and the remaining volume of the carboy was measured in order to calculate volume filtered.&nbsp; Following filtration, filter holders were fit with a short section of tubing and a syringe leur fitting on once side and a 2-way valve on the outflow side. For each filter, 5-ml of 2% glutaraldehyde was slowly injected onto the filter and samples were allowed to fix for 30 minutes. Fixative was drained after this time and 60ml of air was used to flush all filters. Polycarbonate filters were then mounted onto 3x2 glass slides with immersion oil, cover slides were added and the edges of each cover slip was sealed with quick dry nail polish. All slides were stored at -20C and counted within 30 days. Enumeration of diazotrophic taxa was performed using epifluorescence microscopy.The entire slide was counted for DDAs and Trichodesimum abundance. Endosymbiont bearing diatoms of the following genus were enumerated: Rhizosolenia, Hemiaulus, Climacodium and Chaetoceras. Free Richelia intracellularis were also counted. Trichodesmium filaments were counted and the length of each filament was recorded. Trichodesmium cell number was then calculated by dividing the filament length by the mean cell length (9.9 micron +/-2.5 micron).&nbsp;<br />
<br />
<strong>Flow Cytometry Bacteria:</strong><br />
Flow cytometry samples were collected from rosette nisken bottles at specific depths where 3ml of sample volume was pipette into cryovials, preserved by adding 60ul of 2% paraformaldehyde, allowed to sit in the dark for 10 minutes then stored in a -80C until analysis. Post cruise, the samples were thawed in the dark, a 250ul aliquot of sample was removed and added to 250ul Deionized water and 45ul 300mM Potassium citrate, 5ul of SYBR Green stain and 25ul of 1um beads were added, samples were run on a Becton Dickinson FACSCalibur flow cytometer using the methods adpated from Marie, et al 1997. All values are reported in cells/L, NaN are no data.<br />
<br />
<strong>Flow Cytometry-Phytoplankton:</strong><br />
Flow cytometry samples were collected from rosette nisken bottles at specific depths where 3ml of sample volume was pipette into cryovials, preserved by adding 60ul of 2% paraformaldehyde, allowed to sit in the dark for 10 minutes then stored in a -80C until analysis. Post cruise, the samples were thawed in the dark, a 500ul aliquot was removed and 3um beads were added post run, samples were run for 3mins on a Becton Dickinson FACSCalibur flow cytometer using the methods outlined in Sherr, et al 2002.&nbsp;<br />
<br />
<strong>Fluorometric Chlorophyll:</strong><br />
Fluorometric Chlorophyll were collected from rosette nisken bottles at specific depths and filtered at low vacuum through a glass fiber filter. Samples were then flash frozen in liquid nitrogen and stored in a histoprep capsule at -80C until analysis. Post cruise, the filters were extracted using 90% acetone for a total of 48 hours in a -20C freezer, covered to prevent the photodegradation of pigments. Upon extraction samples were centrifuged to settle filter particulates and gently poured into a glass cuvette. Samples were then run on a Turner 10-AU Fluorometer. Data was corrected for average glass fiber filter blank values and where duplicate samples were taken the average and standard deviation are reported. NaN are no data, duplicates not taken.<br />
<br />
<strong>Particulate Carbon (PC) and Particulate Nitrogen (PN):</strong><br />
The carbon, and nitrogen content of particulate material (PC, PN) was collected from rosette nisken bottles at specific depths and filtered at low vacuum through a combusted glass fiber filter (PCPN). Following filtration, filters for PCPN were folded in half and placed in combusted aluminum foil and stored at -80C for later analysis. Post-cruise, all filters were thawed, acidified for a minimum of 12 hours and dried overnight at 60C. Samples were then balled into Ag and Sn boats and plated for analysis. Organic carbon and total nitrogen content was analyzed using high-temperature combustion (1020 C) on a ThermoQuest NC 2500 elemental analyzer. Three standards with known %C and %N were weighed, folded in tin boats, and analyzed with the samples to develop the calibration equation for each run. The calibration equation was then used to calculate the mass (mg) of carbon and nitrogen in each sample using EAS Clarity Chromatography Station software. The standards are Cystine (Elemental Microanalysis, B2035, 11.66 %TN, 29.99 %TC), Atropine (Elemental Microanalysis, B2002, 4.84 %TN, 70.56%TC), and GoniLab Secondary Standard (low %C)-A1 (an internal standard of sediment from the Gulf of Mexico, MV0803, with low carbon - 0.12 %N, 1.3 %C).&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Carlo Erba elemental analyzer (EA)
Carlo Erba elemental analyzer (EA)
PI Supplied Instrument Name: Carlo Erba elemental analyzer (EA) PI Supplied Instrument Description:The carbon and nitrogen isotopic composition (13C and 15N, respectively) were analyzed by continuous-flow isotope ratio mass spectrometry using a Carlo Erba elemental analyzer (EA) connected to a Thermo DeltaPlus isotope ratio mass spectrometer (IRMS). Instrument Name: Carlo-Erba NA-1500 Elemental Analyzer Instrument Short Name:Carlo-Erba NA-1500 Instrument Description: A laboratory instrument that simultaneously determines total nitrogen and total carbon from a wide range of organic and inorganic sediment samples. The sample is completely and instantaneously oxidised by flash combustion, which converts all organic and inorganic substances into combustion products. The resulting combustion gases pass through a reduction furnace and are swept into the chromatographic column by the carrier gas which is helium. The gases are separated in the column and detected by the thermal conductivity detector which gives an output signal proportional to the concentration of the individual components of the mixture. The instrument was originally manufactured by Carlo-Erba, which has since been replaced by Thermo Scientific (part of Thermo Fisher Scientific). This model is no longer in production. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0470/
ThermoQuest NC 2500 elemental analyzer
ThermoQuest NC 2500 elemental analyzer
PI Supplied Instrument Name: ThermoQuest NC 2500 elemental analyzer PI Supplied Instrument Description:Organic carbon and total nitrogen content was analyzed using high-temperature combustion (1020 C) on a ThermoQuest NC 2500 elemental analyzer. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
epifluorescence microscopy
epifluorescence microscopy
PI Supplied Instrument Name: epifluorescence microscopy PI Supplied Instrument Description:Enumeration of diazotrophic taxa was performed using epifluorescence microscopy. Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
Thermo DeltaPlus isotope ratio mass spectrometer (IRMS)
Thermo DeltaPlus isotope ratio mass spectrometer (IRMS)
PI Supplied Instrument Name: Thermo DeltaPlus isotope ratio mass spectrometer (IRMS) PI Supplied Instrument Description:The carbon and nitrogen isotopic composition (13C and 15N, respectively) were analyzed by continuous-flow isotope ratio mass spectrometry using a Carlo Erba elemental analyzer (EA) connected to a Thermo DeltaPlus isotope ratio mass spectrometer (IRMS) Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
nisken bottles
nisken bottles
PI Supplied Instrument Name: nisken bottles PI Supplied Instrument Description:rosette nisken bottles Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: EN596
EN596
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel
R/V Endeavor
Community Standard Description
International Council for the Exploration of the Sea
R/V Endeavor
vessel