http://lod.bco-dmo.org/id/dataset/774585
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-08-06
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental data testing the N2 and CO2 fixing activity of the UCYN-A/haptophyte symbiosis in nitrate and ammonium rich waters in the California Current from May to October 2017
2023-11-29
publication
2023-11-29
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-11-29
publication
https://doi.org/10.26008/1912/bco-dmo.774585.1
Kevin R. Arrigo
Stanford University
principalInvestigator
Jonathan P. Zehr
University of California-Santa Cruz
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Arrigo, K. R., Zehr, J. P. (2023) Experimental data testing the N2 and CO2 fixing activity of the UCYN-A/haptophyte symbiosis in nitrate and ammonium rich waters in the California Current from May to October 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-11-29 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.774585.1 [access date]
UCYN-A/Host activity in DIN-rich waters Dataset Description: <p>Data are from experiments in Southern California Current waters testing the N2 and CO2 fixing activity of the UCYN-A/haptophyte symbiosis in nitrate and ammonium rich waters. Also included are nitrate and ammonium uptake capabilities by the symbiosis. Lastly, CTD sensor data from two cruises on which these experiments were conducted can be found at BCO-DMO dataset CTD sensor (see related datasets).</p> Methods and Sampling: <p>A series of onboard incubations were performed in surface waters of the Southern California coastal system (CCS) to measure bulk community responses (chlorophyll a and POC/PON concentrations), bulk and UCYN-A/haptophyte symbiosis cell-specific N2 fixation and CO2 fixation rates, bulk and UCYN-A/haptophyte symbiosis cell-specific NO3-/NH4+ uptake rates, and UCYN-A/haptophyte symbiosis growth rates under DIN deplete and replete (NO3- or NH4+ amendments) conditions. The experiments were designed to investigate whether UCYN-A continues to fix N2 when NO3- and NH4+ are readily available and if the haptophyte host takes up NO3- and NH4+. Additionally, UCYN-A/haptophyte sublineage-specific responses to NO3-/NH4+ additions were investigated.&nbsp;</p>
<p>Four experimental manipulations were conducted during 2017 and 2018; three experiments (NO3.1, NO3.2, NO3.3) were NO3- addition experiments and one was an NH4+ addition experiment (NH4.1). NO3.1-3 were conducted at three different stations aboard the R/V Gordon Sproul during two research cruises in 2017 that transited off the coast of Southern California and Baja California Sur, Mexico, while NH4.1 was conducted on the Scripps Institute of Oceanography pier.&nbsp;</p>
<p>For NO3.1-3, surface water was pumped into 40 L carboys, housed in an on-deck laboratory container, using a pneumatic diaphragm pump PVDF and Teflon (Wilden Pump and Engineering, Grand Terrace, CA), to allow mixing of the seawater before being randomly dispensed into acid-cleaned 4 L polycarbonate bottles (Thermo Scientific™ Nalgene™, Waltham, MA). Grazers were removed using 150 µm nitex plankton netting (BioQuip, Rancho Dominguez, CA). The bottles were then incubated in triplicate with or without a 2 µmol L-1 addition of NO3- at T0. Incubation bottles were placed in a flow-through surface seawater incubator, amended with neutral density screening to attenuate incident light to 20% of the surface irradiance. Incubations lasted 48 h, with initial rate measurements between 0-24h and final rate measurements between 24-48 h. At each time point, bottles were sacrificed and subsampled for measuring chlorophyll a concentration, particulate nutrient concentrations, bulk CO2 and N2 fixation rates, inorganic N uptake rates, and UCYN-A/haptophyte symbiosis cell-specific N2 fixation, CO2 fixation and NO3- uptake rates (CARD-FISH nanoSIMS). Unlabeled initial samples were used to determine the atom% 15N- and 13C-normal of the unenriched bulk community and UCYN-A/haptophyte symbioses.&nbsp;&nbsp;</p>
<p>For NH4.1, surface water was pumped into 40 L carboys from the waters surrounding the SIO Pier using a pneumatic diaphragm pump PVDF and Teflon (Wilden Pump and Engineering), then randomly dispensed into acid-cleaned 2 L polycarbonate bottles (Thermo Scientific™ Nalgene™). Grazers were removed using 150 µm nitex plankton netting (BioQuip, Rancho Dominguez, CA). The bottles were then incubated with or without a 2 µmol L-1 addition of NH4+ at T0. Incubation bottles were placed in a flow-through surface seawater incubator, amended with neutral screening to attenuate incident light to 20% of the surface irradiance. Incubations lasted 48 h, with N2 fixation initial rate measurements between 0-24h and final rate measurements between 24-48 h. For NH4+ uptake rates, initial rates were measured between 0-6 h, and final rates in NH4+-treatments were measured between 45-51 h. Incubation times (6 h) were chosen to ensure detection of isotope enrichments while minimizing isotope dilution. At each time point, bottles were sacrificed and subsampled for chlorophyll a concentration, particulate nutrient concentrations, bulk CO2 and N2 fixation rates, inorganic N uptake rates, and UCYN-A/haptophyte symbiosis cell-specific N2 fixation, CO2 fixation and NO3- uptake rates (CARD-FISH nanoSIMS). Unlabeled initial samples were used to determine the atom% 15N- and 13C-normal of the unenriched bulk community and UCYN-A/haptophyte symbioses.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559165 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559165
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559152 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559152
completed
Kevin R. Arrigo
Stanford University
650-723-3599
Department of Earth System Science/ Y2E2 Building Room 141
Stanford
CA
94305
USA
arrigo@stanford.edu
pointOfContact
Jonathan P. Zehr
University of California-Santa Cruz
831-459 4009
E&MS A438, Ocean Sciences Department 1156 High Street
Santa Cruz
CA
95064
USA
zehrj@ucsc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Experiment
Cruise
Date
lat
lon
Treatment
n
iso_sub
bulk_fix_uptake_rate_bulk_avg
bulk_fix_uptake_rate_bulk_sd
C_fix_rate_bulk_avg
C_fix_rate_bulk_sd
LOD_bulk
MQR_bulk
sheet
chl_a_n
chla_avg
chla_sd
PON_n
PON_avg
PON_sd
POC_n
POC_avg
POC_sd
Sublineage
sub_enrich_atom_pcnt
sub_enrich_atom_pcnt_sd
fix_uptake_rate_sc_avg
fix_uptake_rate_sc_sd
C_fix_rate_sc_avg
C_fix_rate_sc_sd
LOD_sc
MQR_sc
comment
start_date
end_date
Cameca nanoSIMS 50L
theme
None, User defined
experiment id
Cruise identfier
date
latitude
longitude
treatment
count
sample description
nitrogen fixation rate
rate of 14C fixation
No BCO-DMO term
chlorophyll a
particulate organic nitrogen
particulate organic Carbon (POC)
comments
date_start
date end
featureType
BCO-DMO Standard Parameters
Mass Spectrometer
instrument
BCO-DMO Standard Instruments
SP1714
SP1727
service
Deployment Activity
California Current waters off the coast of Southern California and Baja California
Southern California Current waters
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Biogeochemical significance of the abundant, uncultivated symbiotic cyanobacteria UCYN-A
https://osprey.bco-dmo.org/project/699979
Collaborative Research: Biogeochemical significance of the abundant, uncultivated symbiotic cyanobacteria UCYN-A
<p><em>NSF Award Abstract:</em><br />
Nitrogen is a nutrient whose availability limits growth and productivity of ecosystems. Nitrogen is extremely abundant in the atmosphere in the inert form of gaseous N2, but most organisms cannot reduce N2 into a biologically available form. In all environments, including agricultural soils, there are microorganisms that can make available the N from gaseous N2 by reducing it to the biologically available form, ammonium. In the vast expanses of the open ocean, few organisms are known to have this ability, and recently a unique symbiosis between a single-celled cyanobacterium and a single-celled algae was discovered, which appears to be very widely distributed and likely of global biogeochemical significance. The cyanobacterium in this symbiotic partnership has very unusual metabolism and genomic streamlining. Little is known of the symbiosis because it is not detectable except by modern molecular biological techniques. Recent work has shown this symbiosis to be very widely spread through the oceans, and that there is previously unrecognized diversity in both the cyanobacterial and algal hosts. This research will examine the environmental distributions and the biogeochemical significance of this diversity in coastal US waters. The investigators will engage the public in ocean sciences through internship programs at local high schools and for undergraduate students at Stanford, and by documenting their field research in a 'virtual cruise' blog.</p>
<p>In the marine environment, the contribution of N2 fixation to the fixed nitrogen (N) pool is poorly quantified, in part due to an incomplete understanding on the abundance, activity, and physiology of diazotrophs. The symbiotic unicellular cyanobacteria (UCYN-A) is a poorly characterized, yet globally important, group of marine diazotrophs. UCYN-A is widely distributed in the marine environment, and lives symbiotically with a picoeukaryotic prymnesiophyte alga. We now know that there are multiple ecotypes of UCYN-A, which may be adapted to specific locations in the water-column and different oceanic provinces. Typically N2 fixation was considered unimportant in coastally influenced and non-tropical waters, however recent data shows that multiple subclades of UCYN-A are present. The distribution and rate of N2 fixation by UCYN-A subclades in coastal/nearshore environments is a major unknown in the oceanic N cycle. Its presence in nearshore waters may change the paradigm of the balance between basin N sources (N2 fixation) and sinks (denitrification). Likewise, significant N2 fixation by UCYN-A will need to be considered when determining estimates of new production in coastally influenced waters. This project aims to quantify the significance of different UCYN-A subclades to coastal/nearshore N budgets. It tackles the issue of determining N2 fixation rates by different UCYN-A subclades in coastal waters through rigorous fieldwork off the west coast of North America. The temporal and spatial distribution of UCYN-A subclades, as well as the rates of N2 fixation, will be determined by coupling N2 fixation measurements of bulk communities and individual cells (nanoSIMS) with molecular assays to study these widespread, but dilute, diazotrophic symbionts and their hosts. Additionally the investigators will conduct experiments aimed at constraining the effects of light and nutrient ratios (N/P) on UCYN-A N2 fixation rates, and the prymnesiophyte host's rate of carbon fixation. They will conduct this work through seasonal sampling of a coastal site in the Southern California Bight (Scripps Pier) and on two process cruises in the coastal waters between central California and the Baja Peninsula. The cruise work will provide an opportunity to understand the temporal dynamics of the UCYN-A/prymnesiophyte associations over larger spatial scales. Finally, evidence suggests that unidentified UCYN-A subclades and hosts exist and the investigators have developed a strategy to identify and quantify their temporal and spatial distributions as well as their N2 fixation activities. Data on the coastal distribution, ecology and activity of UCYN-A is critical for obtaining a better understanding of their contribution to fixed N to the marine environment. The group-specific and bulk rates of N2 fixation measured in this study of coastally influenced waters, will provide data for future modeling efforts, which will make an important contribution to constraining oceanic N2 fixation inputs.</p>
BSUCS
largerWorkCitation
project
eng; USA
oceans
California Current waters off the coast of Southern California and Baja California; Southern California Current waters
-117.531
-115.914
28.289
32.867
2017-05-03
2018-05-12
California Current waters off the Southern California shelf
0
BCO-DMO catalogue of parameters from Experimental data testing the N2 and CO2 fixing activity of the UCYN-A/haptophyte symbiosis in nitrate and ammonium rich waters in the California Current from May to October 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/915710.rdf
Name: Experiment
Units: unitless
Description: <p>experiment identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/915711.rdf
Name: Cruise
Units: unitless
Description: <p>cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/915712.rdf
Name: Date
Units: unitless
Description: <p>date coverage of experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/915713.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude with positive values indicating North</p>
http://lod.bco-dmo.org/id/dataset-parameter/915714.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude with negative values indicating West</p>
http://lod.bco-dmo.org/id/dataset-parameter/915715.rdf
Name: Treatment
Units: unitless
Description: <p>treatment description</p>
http://lod.bco-dmo.org/id/dataset-parameter/915716.rdf
Name: n
Units: count
Description: <p>count</p>
http://lod.bco-dmo.org/id/dataset-parameter/915717.rdf
Name: iso_sub
Units: unitless
Description: <p>Isotopic Substrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/915718.rdf
Name: bulk_fix_uptake_rate_bulk_avg
Units: micromole Nitrogen per meter cubed per day (umol N/m3/d)
Description: <p>Average Bulk N2 fixation rate or DIN uptake rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/915719.rdf
Name: bulk_fix_uptake_rate_bulk_sd
Units: micromole Nitrogen per meter cubed per day (umol N/m3/d)
Description: <p>Standard deviation of Bulk N2 fixation rate or DIN uptake rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/915720.rdf
Name: C_fix_rate_bulk_avg
Units: millimole Carbon per meter cubed per day (mmolC/m3/d)
Description: <p>Average Carbon fixation rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/915721.rdf
Name: C_fix_rate_bulk_sd
Units: millimole Carbon per meter cubed per day (mmolC/m3/d)
Description: <p>Standard Deviation of Carbon fixation rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/915722.rdf
Name: LOD_bulk
Units: umol N/m3/d or mmolC/m3/d depending on column it references
Description: <p>Limit of detection, bulk values</p>
http://lod.bco-dmo.org/id/dataset-parameter/915723.rdf
Name: MQR_bulk
Units: umol N/m3/d or mmolC/m3/d depending on column it references
Description: <p>Minimum quantifiable rate, bulk values</p>
http://lod.bco-dmo.org/id/dataset-parameter/915724.rdf
Name: sheet
Units: unitless
Description: <p>name of the original data sheet from which the data was extracted</p>
http://lod.bco-dmo.org/id/dataset-parameter/915725.rdf
Name: chl_a_n
Units: count
Description: <p>count for chlorophyll a measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/915726.rdf
Name: chla_avg
Units: micrograms per liter (ug/L)
Description: <p>Average Chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/915727.rdf
Name: chla_sd
Units: micrograms per liter (ug/L)
Description: <p>Standard deviation of Chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/915728.rdf
Name: PON_n
Units: count
Description: <p>Count of Particulate Organic Nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/915729.rdf
Name: PON_avg
Units: micromole per liter (umol/L)
Description: <p>Average Particulate Organic Nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/915730.rdf
Name: PON_sd
Units: micromole per liter (umol/L)
Description: <p>Standard deviation of Particulate Organic Nitrogen concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/915731.rdf
Name: POC_n
Units: count
Description: <p>Count of Particulate Organic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/915732.rdf
Name: POC_avg
Units: micromole per liter (umol/L)
Description: <p>Average Particulate Organic Carbon concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/915733.rdf
Name: POC_sd
Units: micromole per liter (umol/L)
Description: <p>Standard deviation of Particulate Organic Carbon concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/915734.rdf
Name: Sublineage
Units: unitless
Description: <p>sublineage</p>
http://lod.bco-dmo.org/id/dataset-parameter/915735.rdf
Name: sub_enrich_atom_pcnt
Units: percent
Description: <p>substrate enrichment Atom percent</p>
http://lod.bco-dmo.org/id/dataset-parameter/915736.rdf
Name: sub_enrich_atom_pcnt_sd
Units: percent
Description: <p>standard deviation of substrate enrichment Atom percent</p>
http://lod.bco-dmo.org/id/dataset-parameter/915737.rdf
Name: fix_uptake_rate_sc_avg
Units: femptomole Nitrogen per cell per day (fmol N/cell/day)
Description: <p>Average N2 fixation rate or DIN uptake Rate for single cell rate measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/915738.rdf
Name: fix_uptake_rate_sc_sd
Units: femptomole Nitrogen per cell per day (fmol N/cell/day)
Description: <p>Standard deviation of N2 fixation rate or DIN uptake Rate for single cell rate measurements</p>
http://lod.bco-dmo.org/id/dataset-parameter/915739.rdf
Name: C_fix_rate_sc_avg
Units: femptomole Carbon per cell per day (fmol C/cell/day)
Description: <p>Average Carbon fixation rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/915740.rdf
Name: C_fix_rate_sc_sd
Units: femptomole Carbon per cell per day (fmol C/cell/day)
Description: <p>Standard deviation of Carbon fixation rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/915741.rdf
Name: LOD_sc
Units: fmol N/cell/day or fmol C/cell/day depending on column it references
Description: <p>Limit of detection, single cell values</p>
http://lod.bco-dmo.org/id/dataset-parameter/915742.rdf
Name: MQR_sc
Units: unknown
Description: <p>Minimum quantifiable rate, single cell values</p>
http://lod.bco-dmo.org/id/dataset-parameter/915743.rdf
Name: comment
Units: unitless
Description: <p>additional comments related to the data</p>
http://lod.bco-dmo.org/id/dataset-parameter/915744.rdf
Name: start_date
Units: unitless
Description: <p>start date of experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/915745.rdf
Name: end_date
Units: unitless
Description: <p>end date of experiment</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10514
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/383da31a-e325-4a11-9ce9-0d9eb729a781/content
download
https://doi.org/10.26008/1912/bco-dmo.774585.1
download
onLine
dataset
<p>A series of onboard incubations were performed in surface waters of the Southern California coastal system (CCS) to measure bulk community responses (chlorophyll a and POC/PON concentrations), bulk and UCYN-A/haptophyte symbiosis cell-specific N2 fixation and CO2 fixation rates, bulk and UCYN-A/haptophyte symbiosis cell-specific NO3-/NH4+ uptake rates, and UCYN-A/haptophyte symbiosis growth rates under DIN deplete and replete (NO3- or NH4+ amendments) conditions. The experiments were designed to investigate whether UCYN-A continues to fix N2 when NO3- and NH4+ are readily available and if the haptophyte host takes up NO3- and NH4+. Additionally, UCYN-A/haptophyte sublineage-specific responses to NO3-/NH4+ additions were investigated.&nbsp;</p>
<p>Four experimental manipulations were conducted during 2017 and 2018; three experiments (NO3.1, NO3.2, NO3.3) were NO3- addition experiments and one was an NH4+ addition experiment (NH4.1). NO3.1-3 were conducted at three different stations aboard the R/V Gordon Sproul during two research cruises in 2017 that transited off the coast of Southern California and Baja California Sur, Mexico, while NH4.1 was conducted on the Scripps Institute of Oceanography pier.&nbsp;</p>
<p>For NO3.1-3, surface water was pumped into 40 L carboys, housed in an on-deck laboratory container, using a pneumatic diaphragm pump PVDF and Teflon (Wilden Pump and Engineering, Grand Terrace, CA), to allow mixing of the seawater before being randomly dispensed into acid-cleaned 4 L polycarbonate bottles (Thermo Scientific™ Nalgene™, Waltham, MA). Grazers were removed using 150 µm nitex plankton netting (BioQuip, Rancho Dominguez, CA). The bottles were then incubated in triplicate with or without a 2 µmol L-1 addition of NO3- at T0. Incubation bottles were placed in a flow-through surface seawater incubator, amended with neutral density screening to attenuate incident light to 20% of the surface irradiance. Incubations lasted 48 h, with initial rate measurements between 0-24h and final rate measurements between 24-48 h. At each time point, bottles were sacrificed and subsampled for measuring chlorophyll a concentration, particulate nutrient concentrations, bulk CO2 and N2 fixation rates, inorganic N uptake rates, and UCYN-A/haptophyte symbiosis cell-specific N2 fixation, CO2 fixation and NO3- uptake rates (CARD-FISH nanoSIMS). Unlabeled initial samples were used to determine the atom% 15N- and 13C-normal of the unenriched bulk community and UCYN-A/haptophyte symbioses.&nbsp;&nbsp;</p>
<p>For NH4.1, surface water was pumped into 40 L carboys from the waters surrounding the SIO Pier using a pneumatic diaphragm pump PVDF and Teflon (Wilden Pump and Engineering), then randomly dispensed into acid-cleaned 2 L polycarbonate bottles (Thermo Scientific™ Nalgene™). Grazers were removed using 150 µm nitex plankton netting (BioQuip, Rancho Dominguez, CA). The bottles were then incubated with or without a 2 µmol L-1 addition of NH4+ at T0. Incubation bottles were placed in a flow-through surface seawater incubator, amended with neutral screening to attenuate incident light to 20% of the surface irradiance. Incubations lasted 48 h, with N2 fixation initial rate measurements between 0-24h and final rate measurements between 24-48 h. For NH4+ uptake rates, initial rates were measured between 0-6 h, and final rates in NH4+-treatments were measured between 45-51 h. Incubation times (6 h) were chosen to ensure detection of isotope enrichments while minimizing isotope dilution. At each time point, bottles were sacrificed and subsampled for chlorophyll a concentration, particulate nutrient concentrations, bulk CO2 and N2 fixation rates, inorganic N uptake rates, and UCYN-A/haptophyte symbiosis cell-specific N2 fixation, CO2 fixation and NO3- uptake rates (CARD-FISH nanoSIMS). Unlabeled initial samples were used to determine the atom% 15N- and 13C-normal of the unenriched bulk community and UCYN-A/haptophyte symbioses.</p>
Specified by the Principal Investigator(s)
<p>NanoSIMS images were processed using Look@nanosims software.</p>
<p>BCO-DMO Processing Notes:<br />
-&nbsp;added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- combined sheets "Bulk Rate measurements", "Bulk_Biomass", and "Single Cell Rate Measurements"<br />
- added a column for sheet name<br />
- extracted&nbsp;start_date and end_date columns from Date column.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Cameca nanoSIMS 50L
Cameca nanoSIMS 50L
PI Supplied Instrument Name: Cameca nanoSIMS 50L PI Supplied Instrument Description:Samples for single cell N2 fixation and CO2 fixation rates were analyzed using a Cameca nanoSIMS 50L (https://www.cameca.com/products/sims/nanosims) located at Stanford University’s nano shared facilities (SNSF, https://snsf.stanford.edu/equipment/xsa/nanosims.html). Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Cruise: SP1714
SP1714
R/V Robert Gordon Sproul
Community Standard Description
International Council for the Exploration of the Sea
R/V Robert Gordon Sproul
vessel
SP1714
Matthew M. Mills
Stanford University
Cruise: SP1727
SP1727
R/V Robert Gordon Sproul
Community Standard Description
International Council for the Exploration of the Sea
R/V Robert Gordon Sproul
vessel
SP1727
Matthew M. Mills
Stanford University
R/V Robert Gordon Sproul
Community Standard Description
International Council for the Exploration of the Sea
R/V Robert Gordon Sproul
vessel