http://lod.bco-dmo.org/id/dataset/774639 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2019-08-06 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Suspended aggregates of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017 2019-08-13 publication 2019-08-13 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2019-12-24 publication https://doi.org/10.1575/1912/bco-dmo.774639.1 Susanne Neuer Arizona State University principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Cruz, B. (2019) Suspended aggregates of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-08-13 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.774639.1 [access date] Dataset Description: &lt;p&gt;Suspended aggregates of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017.&lt;/p&gt; &lt;p&gt;&lt;br /&gt; These data were published in&amp;nbsp;Cruz and Neuer, 2019.&lt;br /&gt; &lt;br /&gt; Related datasets from the same laboratory experiments:&lt;br /&gt; Picocyanobacteria TEP: Cell Abundance https://www.bco-dmo.org/dataset/774628&lt;br /&gt; Picocyanobacteria TEP: Growth Rates https://www.bco-dmo.org/dataset/774634&lt;br /&gt; Picocyanobacteria TEP: TEP Concentration https://www.bco-dmo.org/dataset/774646&lt;/p&gt; Methods and Sampling: &lt;p&gt;Methodology:&lt;br /&gt; Duplicate cultures of Synechococcus WH8102 (axenic), Synechococcus WH7805 (xenic) and Prochlorococcus marinus MED4 (xenic and axenic) were sampled every other day for 17-19 days for the quantification of single cells, suspended aggregates (aggregates ca. 5-60 μm), and TEP.&amp;nbsp; Suspended aggregates were quantified using a Multisizer 3 Particle Counter.&amp;nbsp;&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1658527 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1658527 completed Susanne Neuer Arizona State University 480-727-7254 College of Liberal Arts and Sciences School of Life Sciences 427 East Tyler Mall Tempe AZ 85287-4501 USA susanne.neuer@asu.edu pointOfContact asNeeded Dataset Version: 1 Unknown Culture Days Suspended_Aggregate_Volume_Concentration_rep1 Suspended_Aggregate_Volume_Concentration_rep2 Multisizer 3 Particle Counter, Beckman Coulter, CA theme None, User defined sample description time_elapsed featureType BCO-DMO Standard Parameters Particle Size Analyzer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Aggregation of Marine Picoplankton https://osprey.bco-dmo.org/project/710239 Aggregation of Marine Picoplankton <p>NSF abstract:</p> <p>Marine phytoplankton are microscopic algae that live in the sunlit zone of the ocean. They play an important role in the uptake of carbon dioxide from the atmosphere through photosynthesis, similar to what plants do on land, and are the basis of the marine food web. However, instead of storing this organic carbon in leaf tissue and roots, marine phytoplankton are grazed by planktonic animals, or die and subsequently sink out of the sunlit zone in the form of aggregates, also called "Marine Snow". These particles not only export the organic carbon contained in their cells to the deep ocean, but also serve as food for animals and bacteria that live in the deep. A considerable portion of these phytoplankton are extremely small, among the tiniest of all organisms known. These extremely small cells have not been thought to play an important role in the formation and sinking of marine snow; however, recent findings challenge this view. This project will investigate how the smallest of these phytoplankton contribute to the rain of sinking particles from the sunlit surface to the deep ocean. This research is important because, in some of the largest expanses of the open oceans, these minute cells dominate the phytoplankton community, and larger plankton organisms are very sparse. The project, through a combination of work in the laboratory and at a field station, will shed light on how these tiny phytoplankton cells make aggregates, which ultimately enable them to sink as "Marine Snow". The project also provides unique opportunities for undergraduate students at Arizona State University, a land-locked public university, to gain experience in working with marine research. The project will serve to educate one PhD student, one MS student in an accelerated BS-MS program, and 8-10 undergraduate students/semester in a unique, inquiry based learning effort termed Microbial EducatioN Training and OutReach (MENTOR). The undergraduate students will also participate in Arizona State University (ASU)'s School of Life Sciences, Undergraduate Research Program (SOLUR), which seeks to increase the participation of minorities in science. They will also contribute towards developing web and classroom materials, based on this project, which will then be distributed through a partnership with the award-winning ASU-sponsored Ask A Biologist K-12 web site.</p> <p>The oceanic "biological carbon pump", the photosynthetically mediated transformation of dissolved inorganic carbon into particulate and dissolved organic carbon and its subsequent export to deep water, functions as a significant driver of atmospheric carbon uptake by the oceans. The traditional view of the biological carbon pump in the ocean is that of sinking of large aggregates (marine snow) or fecal pellets, which are made up of large, mineral ballasted cells of phytoplankton. However, recent evidence, stemming from in situ investigations of particulate matter, trap studies and modelling studies, have shown that micron-sized phytoplankton such as picocyanobacteria as well as picoeukaryotes can contribute significantly to the sinking of particulate matter. The specific mechanisms behind the sinking of these micrometer sized cells remain elusive as the cells are too small to sink on their own, and mesozooplankton is likely unable to ingest single cells. Intriguingly, recent research by the investigators has shown that the ubiquitous picocyanobacteria Synechococcus are able to form aggregates and sink at velocities comparable to those of marine snow. They found that the matrix of the Synechococcus aggregates was made of Transparent Exopolymeric Particles (TEP), and that TEP production was enhanced under nutrient limited culture conditions. Interaction with clays and presence of heterotrophic bacteria also enhanced aggregation and sinking velocity. This study aims to further investigate aggregation of other common picoplankton in the laboratory and aggregation occurring in natural settings at an oligotrophic open ocean site, the Bermuda Atlantic Time-series Site (BATS). Ultimately, this project will increase and refine our understanding of the role of the smallest phytoplankton in aggregation and sinking - information vital to understanding carbon cycling processes in the oceans.</p> Marine Plankton Aggregation largerWorkCitation project eng; USA oceans 2016-01-01 2017-01-01 Bermuda Atlantic Time-Series station 0 BCO-DMO catalogue of parameters from Suspended aggregates of xenic and axenic marine Prochlorococcus and Synechococcus cultures from laboratory experiments conducted in 2016 and 2017 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/775466.rdf Name: Culture Units: unitless Description: <p>Culture name</p> http://lod.bco-dmo.org/id/dataset-parameter/775467.rdf Name: Days Units: count (days) Description: <p>Days since experiment start</p> http://lod.bco-dmo.org/id/dataset-parameter/775468.rdf Name: Suspended_Aggregate_Volume_Concentration_rep1 Units: x10^6 μm^3 per mL Description: <p>Volume concentration of suspended aggregates for replicate 1 with an equivalent spherical diameter of 5-60 μm.</p> http://lod.bco-dmo.org/id/dataset-parameter/775469.rdf Name: Suspended_Aggregate_Volume_Concentration_rep2 Units: x10^6 μm^3 per mL Description: <p>Volume concentration of suspended aggregates for replicate 2 with an equivalent spherical diameter of 5-60 μm.</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 1479 https://darchive.mblwhoilibrary.org/bitstream/1912/25052/1/dataset-774639_picocyanobacteria-tep-suspended-aggregates__v1.tsv download https://doi.org/10.1575/1912/bco-dmo.774639.1 download onLine dataset &lt;p&gt;Methodology:&lt;br /&gt; Duplicate cultures of Synechococcus WH8102 (axenic), Synechococcus WH7805 (xenic) and Prochlorococcus marinus MED4 (xenic and axenic) were sampled every other day for 17-19 days for the quantification of single cells, suspended aggregates (aggregates ca. 5-60 μm), and TEP.&amp;nbsp; Suspended aggregates were quantified using a Multisizer 3 Particle Counter.&amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;BCO-DMO Data Manager Processing Notes: Growth Rates&lt;br /&gt; * Excel sheet extracted to a csv file&lt;br /&gt; * added a conventional header with dataset name, PI name, version date&lt;br /&gt; * modified parameter names to conform with BCO-DMO naming conventions&lt;br /&gt; * blank values in this dataset are displayed as &quot;nd&quot; for &quot;no data.&quot;&amp;nbsp; nd is the default missing data identifier in the BCO-DMO system.&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Multisizer 3 Particle Counter, Beckman Coulter, CA Multisizer 3 Particle Counter, Beckman Coulter, CA PI Supplied Instrument Name: Multisizer 3 Particle Counter, Beckman Coulter, CA Instrument Name: Particle Size Analyzer Instrument Short Name: Instrument Description: Particle size analysis, particle size measurement, or simply particle sizing is the collective name of the technical procedures, or laboratory techniques which determines the size range, and/or the average, or mean size of the particles in a powder or liquid sample.