http://lod.bco-dmo.org/id/dataset/774855
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-08-07
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Ammonium, total nitrate and nitrite, nitrite, and flow cytometry profiles in the Eastern Tropical North Pacific from March to April 2018
2021-04-06
publication
2021-04-06
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-04-23
publication
https://doi.org/10.26008/1912/bco-dmo.774855.1
Bess B. Ward
Princeton University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Ward, B. B. (2021) Ammonium, total nitrate and nitrite, nitrite, and flow cytometry profiles in the Eastern Tropical North Pacific from March to April 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-04-06 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.774855.1 [access date]
Methods and Sampling: <p>All samples were taken using Niskin bottles during CTD casts.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1657663 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1657663
completed
Bess B. Ward
Princeton University
609-258-5150
Geosciences Department 217 Guyot Hall
Princeton
NJ
08544-1003
USA
bbw@princeton.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Local_Time
UTC_Time
Station
Latitude
Longitude
CTD
Niskin
Depth
Ammonium
Nitrite
Nitrite_Nitrate
Chlpos
HetBact
PEneg
PEpos
Picoeuk
Prochl
Syn
Air_Temp
Log_Taker
Events_Notes
Teledyne Instruments Chemiluminescence NO/NOx Analyzer
CTD Sea-Bird 9
BD Accuri C6 Flow Cytometer
Turner Designs fluorometer Model: 7200-000
theme
None, User defined
date
sample time
ISO_DateTime_UTC
station
latitude
longitude
cast
Niskin bottle number
depth
Ammonium
Nitrite
nitrate plus nitrite
cell_concentration
temperature of sample or a generic measurement (not in-situ water temp)
sample description
featureType
BCO-DMO Standard Parameters
Chemiluminescence NOx Analyzer
CTD Sea-Bird 9
Flow Cytometer
Turner Designs 700 Laboratory Fluorometer
UV Spectrophotometer-Shimadzu
instrument
BCO-DMO Standard Instruments
SR1805
service
Deployment Activity
Eastern Tropical North Pacific Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Mechanisms and Controls of Nitrous Oxide Production in the Eastern Tropical North Pacific Ocean
https://osprey.bco-dmo.org/project/741018
Collaborative Research: Mechanisms and Controls of Nitrous Oxide Production in the Eastern Tropical North Pacific Ocean
<p><em>NSF Award Abstract:</em><br />
Nitrous oxide (N2O) is present at very low concentrations in the atmosphere but is an important greenhouse gas and ozone destroying substance. As with other climate-active gases like methane and carbon dioxide, human activities are responsible for most of its production, either directly through fossil fuel burning or agricultural activities. However, about a third of natural N2O emissions come from the ocean, but even these emissions can be indirectly affected by human activities. About half of the ocean source is derived from three specific geographic regions in the Pacific Ocean and Arabian Sea. These three oceanic regions are places where oxygen concentrations are so low in the intermediate depths that metabolic processes requiring the absence of oxygen are able to occur. These regions are called Oxygen Minimum Zones (OMZs) and they have microbiological processes that occur nowhere else in global ocean waters. In the work proposed here, we will investigate how the microbiological pathways of N2O production and consumption are regulated by environmental conditions such as oxygen and nutrient concentration. This work will involve a research expedition to one of the OMZs, the Eastern Tropical Pacific Ocean off the coast of Mexico. On the cruise, we will perform experiments and collect samples for analysis in our home laboratories at Princeton and Stanford Universities. Advising of graduate students and teaching at the graduate and undergraduate levels at both institutions will be linked to this research. This work is particularly timely because global warming has already indirectly affected the size and geographic extent of the OMZs. Greater expanse of low oxygen water could cause N2O production to increase, leading to increased fluxes of N2O to the atmosphere. In the atmosphere, the role of N2O in ozone destruction and as a greenhouse gas could be critical elements of global change.</p>
<p>Nitrous oxide (N2O) is an important greenhouse gas and ozone destroying substance. About a third of natural N2O emissions come from the ocean, and about half of the ocean source is derived from waters with oxygen deficient intermediate waters (oxygen minimum zones, OMZs). Nitrification is recognized as the main source of N2O in the ocean, but denitrification also likely contributes to the net source in and around OMZs. Because nitrification and denitrification are performed by microbes with very different metabolisms and environmental controls, their contributions to N2O production are expected to differ in response to changes in oxygenation and nutrient inputs. Thus it is important to understand the regulation of N2O production by both processes. The main goal of this project is to quantify the environmental regulation of N2O production and consumption pathways in and around OMZs in order to obtain predictive understanding of N2O distributions and fluxes in the ocean. To do this, production and consumption of N2O will be measured using stable isotope tracer incubations at stations located within and outside one of the major OMZs in the Eastern Tropical North Pacific ocean. The dependence of the rate processes on substrate, product, and oxygen concentrations will be determined, and the composition of the microbial assemblages will be assessed to determine whether different microbial components are involved under different environmental conditions. Natural abundance stable isotope and isotopomer measurements of N2O will be interpreted in concert with measured rates to deduce the sources and pathways (nitrification, nitrifier-denitrification, denitrification, and ?hybrid? formation) involved in N2O production and consumption. This work will also involve a novel application of isotopomer measurements of N2O from incubations to identify the placement of 15N from NH4+ and NO2- within labeled N2O pools.</p>
<p>OMZ regions are the sites of unique nitrogen cycling processes that are critical in determining the fixed nitrogen inventory of the ocean. If OMZs expand as predicted due to anthropogenic changes in the coming decades, changes in these chemical distributions may affect the atmospheric flux of nitrous oxide as well as modify overall ocean productivity via changes in the fixed nitrogen inventory. Understanding the regulation and environmental control of the processes responsible for N2O production and consumption is the foundation of understanding their response to global change.</p>
N2O in ETNP
largerWorkCitation
project
eng; USA
oceans
Eastern Tropical North Pacific Ocean
-114.7916
-102.3498
9.9998
19.6232
2018-03-16
2018-04-12
Eastern Tropical North Pacific Ocean (oxygen minimum zone)
0
BCO-DMO catalogue of parameters from Ammonium, total nitrate and nitrite, nitrite, and flow cytometry profiles in the Eastern Tropical North Pacific from March to April 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/847801.rdf
Name: Date
Units: unitless
Description: <p>Date yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/847802.rdf
Name: Local_Time
Units: unitless
Description: <p>Local time (hh:mm). Note: Local time zones changed between CTD23 and CTD24 and again between CTD66 and CTD67.</p>
http://lod.bco-dmo.org/id/dataset-parameter/847803.rdf
Name: UTC_Time
Units: unitless
Description: <p>UTC time (hh:mm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/847804.rdf
Name: Station
Units: unitless
Description: <p>Station designation</p>
http://lod.bco-dmo.org/id/dataset-parameter/847805.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of CTD cast, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/847806.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of CTD cast, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/847807.rdf
Name: CTD
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/847808.rdf
Name: Niskin
Units: unitless
Description: <p>bottle number</p>
http://lod.bco-dmo.org/id/dataset-parameter/847809.rdf
Name: Depth
Units: meters (m)
Description: <p>sampe depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/847810.rdf
Name: Ammonium
Units: nanomolar (nM)
Description: <p>ammonium (NH4) concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/847811.rdf
Name: Nitrite
Units: micromolar (uM)
Description: <p>nitrite (NO2) concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/847812.rdf
Name: Nitrite_Nitrate
Units: micromolar (uM)
Description: <p>Nitrite plus nitrate concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/847813.rdf
Name: Chlpos
Units: cells per milliliter
Description: <p>chlorophyll positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/847814.rdf
Name: HetBact
Units: cells per milliliter
Description: <p>Heterotrophic bacterial cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/847815.rdf
Name: PEneg
Units: cells per milliliter
Description: <p>Phycoerythrin negative cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/847816.rdf
Name: PEpos
Units: cells per milliliter
Description: <p>Phyoerythrin positive cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/847817.rdf
Name: Picoeuk
Units: cells per milliliter
Description: <p>Picoeukaryote cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/847818.rdf
Name: Prochl
Units: cells per milliliter
Description: <p>Prochlorococcus cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/847819.rdf
Name: Syn
Units: cells per milliliter
Description: <p>Synechococcus cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/847820.rdf
Name: Air_Temp
Units: degrees celsius (°C)
Description: <p>Air temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/847821.rdf
Name: Log_Taker
Units: unitless
Description: <p>Person in charge of the log for that event.</p>
http://lod.bco-dmo.org/id/dataset-parameter/847822.rdf
Name: Events_Notes
Units: unitless
Description: <p>Event type, brief description of event</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
52534
https://darchive.mblwhoilibrary.org/bitstream/1912/26996/1/dataset-774855_profile-data__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.774855.1
download
onLine
dataset
<p>All samples were taken using Niskin bottles during CTD casts.</p>
Specified by the Principal Investigator(s)
<p>Cell abundances: Cell abundances were analyzed using flow cytometry as previously described (Van Oostende et al. 2017). Samples were collected in 5 mL-cryovials from 30-L Niksin bottles and fixed with 0.1% glutaraldehyde and frozen at -80C until later analysis in the shore based laboratory.&nbsp;</p>
<p>Ammonium: Ammonium concentration was measured manually fluorometrically using standard methods (Holmes et al 1999). Water was collected using Niskin sampling bottles.&nbsp; Samples were measured immediately upon retrieval and were not filtered prior to analysis. Five ml volumes were analyzed.&nbsp;</p>
<p>Nitrite: Nitrite concentration was measured manually colorimetrically using standard methods (Strickland and Parsons 1972). Water was collected using Niskin sampling bottles.&nbsp; Samples were measured immediately upon retrieval and were not filtered prior to analysis. Five ml volumes were analyzed.</p>
<p>Nitrite + Nitrate: Nitrite + Nitrate (NOx) concentration was measured using the chemiluminescence method (Garside 1982)<br />
Water was collected using Niskin sampling bottles.&nbsp; Water was dispensed into 12-ml detainer vials and used in incubation experiments.&nbsp; Incubations were terminated by addition of saturated ZnCl2 and returned to the shore based laboratory. After mass spec analysis of the N2 gas in the vials, they were subsampled for analysis of total NOx in solution.</p>
<p>&nbsp;</p>
<p>BCO-DMO Processing Notes:</p>
<p>- Combined NH4, NO2, Nitrate+Nitrate and flow cytometer datasets<br />
-&nbsp;added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- combined&nbsp;the degrees and minutes columns of lat and long values to create&nbsp;lat and lon columns in decimal degrees, rounded columns to 6 digits.<br />
&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Teledyne Instruments Chemiluminescence NO/NOx Analyzer
Teledyne Instruments Chemiluminescence NO/NOx Analyzer
PI Supplied Instrument Name: Teledyne Instruments Chemiluminescence NO/NOx Analyzer PI Supplied Instrument Description:Chemiluminescence was measured on 200 µl samples using a Teledyne Instruments Chemiluminescence NO/NOx Analyzer – 10 Model 200E (NOx Box) Instrument Name: Chemiluminescence NOx Analyzer Instrument Short Name: Instrument Description: The chemiluminescence method for gas analysis of oxides of nitrogen relies on the measurement of light produced by the gas-phase titration of nitric oxide and ozone. A chemiluminescence analyzer can measure the concentration of NO/NO2/NOX.
One example is the Teledyne Model T200: https://www.teledyne-api.com/products/nitrogen-compound-instruments/t200
CTD Sea-Bird 9
CTD Sea-Bird 9
PI Supplied Instrument Name: CTD Sea-Bird 9 PI Supplied Instrument Description:CTD: Sea-Bird 9. CTD data processed with Seasave V7.26.7.107 Instrument Name: CTD Sea-Bird 9 Instrument Short Name:CTD SBE 9 Instrument Description: The Sea-Bird SBE 9 is a type of CTD instrument package. The SBE 9 is the Underwater Unit and is most often combined with the SBE 11 Deck Unit (for real-time readout using conductive wire) when deployed from a research vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorometer, altimeter, etc.). Note that in most cases, it is more accurate to specify SBE 911 than SBE 9 since it is likely a SBE 11 deck unit was used. more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
BD Accuri C6 Flow Cytometer
BD Accuri C6 Flow Cytometer
PI Supplied Instrument Name: BD Accuri C6 Flow Cytometer PI Supplied Instrument Description:A BD Accuri C6 Flow Cytometer was used for the enumeration. Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Turner Designs fluorometer Model: 7200-000
Turner Designs fluorometer Model: 7200-000
PI Supplied Instrument Name: Turner Designs fluorometer Model: 7200-000 PI Supplied Instrument Description:Fluorescence was measured on a Turner Designs fluorometer Model: 7200-000 using a 1 cm cell. Instrument Name: Turner Designs 700 Laboratory Fluorometer Instrument Short Name:TD-700 Instrument Description: The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/
PI Supplied Instrument Name: PI Supplied Instrument Description:Color (Nitrite concentration) was measured on a Shimadzu UV Spectrophotometer, Model: UV-1800 120V using a 10 cm cell. Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: SR1805
SR1805
R/V Sally Ride
Community Standard Description
International Council for the Exploration of the Sea
R/V Sally Ride
vessel
SR1805
Bess B. Ward
Princeton University
R/V Sally Ride
Community Standard Description
International Council for the Exploration of the Sea
R/V Sally Ride
vessel