19:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1a:Tf9d,NSF Award Abstract: Coral reefs, the tropical rain forests of the marine environment, are under significant threat from a variety of stressors such as pollution, overfishing, coastal development and climate change. There is increasing interest by the coral reef research community in the ecology and evolution of other groups of organisms besides corals on coral reefs with sponges being of particular interest. Sponges are a very old group of organisms essential to reef health because of their roles in nutrient cycling, providing food and homes for many other reef organisms, and their ability to synthesize diverse chemical compounds of ecological importance on the reef, and of interest to the biomedical community. Many of these important functions would not be possible without the symbiotic microbes (e.g., bacteria) that live within sponges. In this project, the investigators will examine relationships between the sponge host and its microbiome in the ecological roles described above. Like the human microbiome, understanding the sponge micobiome may be the key to understanding their ecology and biodiversity. The investigators will use a combination of classical ecological approaches combined with sophisticated biochemical and molecular analyses to unravel the role of the symbionts in the ecology and evolution of sponges. Both the University of New Hampshire and the University of Mississippi will provide training opportunities for undergraduate and graduate students as well as veterans and post-doctoral researchers, especially from underrepresented groups. Additionally, the investigators will develop unique outreach programs for public education on the importance of coral reef ecosystems. The goal of this study is to examine the relationships between marine sponges and their microbiomes, and reveal the phylogenetic, genetic, and functional biodiversity of coral reef sponges across the Caribbean basin. This research will provide a better understanding of sponges as a major functional component of the biodiversity of coral reef communities. This transformative project will examine important paradigms relative to sponge communities worldwide that will provide unique insights into the integrative biodiversity of sponges on coral reefs and enhance our understanding of the ecology and evolution of this extensive, yet understudied, group of marine organisms. This is essential because sponges continue to emerge as the dominant taxon on many coral reefs, particularly following regional declines in coral cover over the last three decades, and their ecological importance to shallow coral reef communities is unequivocal. In addition, many marine sponges host a diverse assemblage of symbiotic microorganisms that play critical functional roles in nutrient cycling within sponges themselves and in the coral reef communities where they reside, and many sponges can potentially transfer photoautotrophically derived energy to higher trophic levels. As shallow coral reefs continue to decline, the phylogenetic, genetic, and functional diversity of coral reefs will increasingly be found in taxa other than scleractinian corals, such as soft corals and sponges. The investigators predict that co-evolution of the sponge host and microbiome leads to emergent functional properties that result in niche diversification and speciation of sponges. To assess this, they will quantify trophic modes (e.g., DOM and POC uptake, photo-autotrophy) of sponges in the Caribbean, as well as the production of chemical defenses. These character states will be analyzed in the context of the phylogenetic composition of the sponge hosts and their microbiomes, and the functional activities of the host and symbionts at the genetic level (i.e., transcriptomics and metatranscriptomics). These data will provide unique insights into the co-evolution of sponges and their microbiomes, and how these symbioses influence the functional attributes of sponges within coral reef communities.6:["$","$L19",null,{"dataset":{"_id":"775451","description":"

This dataset includes NCBI Accessions for Raw sequence data for 16S rRNA amplicon microbiomes.

The NCBI BioProject number is PRJNA555077 which covers 1547 samples of 16S libraries where the metadata are also available but the sequences themselves are still embargoed. [2019-08-15]

Samples were collected at 15 m by SCUBA diving in the Caribbean near Curacao, Belize, Cayman Islands and St. Croix, in 2009 and 2017-2018. Samples were cleaned, frozen whole, or placed in a DNA/RNA preservation buffer and shipped back to the University of New Hampshire where DNA was extracted and metagenetic, shotgun or cDNA sequencing was conducted.

Data was quality controlled and analyzed in spreadsheets for descriptive statistics, and statistical software was used for bioinformatic analyses.

\n","problem":"","acquisition":"","processing":"

BCO-DMO Processing Notes:
\n- data were downloaded from NCBI BioProject PRJNA555077 on 2019-08-14 [https://www.ncbi.nlm.nih.gov/bioproject/PRJNA555077] and reformatted as a flat table.
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions

\n","bcodmoProcessing":"","title":"NCBI accessions and metadata associated with Caribbean sponge metagenomes collected from Curacao, Belize, Cayman Islands and St. Croix, 2009 and 2017-2018","nickname":"Sponge holobiont accessions and metadata","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"1","version_date":"2019-08-15T04:00:00.000Z","abstract":"NCBI accessions and metadata associated with Caribbean sponge metagenomes collected from Curacao, Belize, Cayman Islands and St. Croix, 2009 and 2017-2018.","citation":"Lesser, M., Slattery, M. (2019) NCBI accessions and metadata associated with Caribbean sponge metagenomes collected from Curacao, Belize, Cayman Islands and St. Croix, 2009 and 2017-2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-08-15 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.775451.1 [access date]","citationParts":{"identifier":"doi:10.1575/1912/bco-dmo.775451.1","versionDate":"2019-08-15","versionId":"1","year":"2019","title":"NCBI accessions and metadata associated with Caribbean sponge metagenomes collected from Curacao, Belize, Cayman Islands and St. Croix, 2009 and 2017-2018","authors":"Lesser, M., Slattery, M.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.1575/1912/bco-dmo.775451.1","release_date":null,"field_dataset_location":"","temporal_extent":{"start":"2009-01-01T05:00:00.000Z","end":"2018-08-10T04:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1230786000","label":"2009-01-01"},"end":{"value":"1533873600","label":"2018-08-10"}},"spatial_extent":{"shape":"polygon","center":{"latitude":15.74255,"longitude":-76.3806505},"wkt":"POLYGON ((-88.0878 12.0828, -64.673501 12.0828, -64.673501 19.4023, -88.0878 19.4023, -88.0878 12.0828))","north":19.4023,"east":-64.673501,"south":12.0828,"west":-88.0878},"dataset_type":["Other Field Results"],"keywords":["biota","oceans"],"data_files":[{"id":"902615","title":"sponge_accessions.csv","url":"https://datadocs.bco-dmo.org/dataset/775451/file/yppzB6NhmQJVV/sponge_accessions.csv","showTableUrl":"https://www.bco-dmo.org/dataset/775451/data/view/902615","description":"Primary data file for dataset ID 775451","filename":"sponge_accessions.csv","bytesize":208227,"checksum":{"value":"4d0a47e9493f47c52737085eb3f7c258","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"bcodmo_dataset_775451","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"645511","name":"Michael P. Lesser"},"performedAtOrganization":{"_id":"33","name":"University of New Hampshire"}},{"role":{"_id":"140","name":"Co-Principal Investigator"},"performedBy":{"_id":"647901","name":"Marc Slattery"},"performedAtOrganization":{"_id":"647900","name":"University of Mississippi"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"50396","name":"Nancy Copley"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[],"funding":[{"_id":"649060","name":"OCE-1638296","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1638296"}],"instruments":[{"_id":"775458","name":"","description":"","type_id":"649","type_name":"Automated DNA Sequencer","type_desc":"A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences.","type_acronym":"Automated Sequencer"},{"_id":"775508","name":"in situ light sensor","description":"","type_id":"703","type_name":"Light Meter","type_desc":"Light meters are instruments that measure light intensity. Common units of measure for light intensity are umol/m2/s or uE/m2/s (micromoles per meter squared per second or microEinsteins per meter squared per second). (example: LI-COR 250A)","type_acronym":"Light Meter"},{"_id":"775507","name":"in situ temperature sensor","description":"","type_id":"725867","type_name":"Thermometer","type_desc":"A device designed to measure temperature.","type_acronym":""}],"parameters":[{"_id":"775489","name":"BioProject","format":"","frictionlessType":"","description":"

NCBI BioProject accession number

","units":{"_id":"","name":"unitless"},"type":{"_id":"1971","name":"accession number","description":"

Database identifier assigned by repository and linked to GenBank or other repository.

"}},{"_id":"775490","name":"BioSample","format":"","frictionlessType":"","description":"

NCBI BioSample accession number

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Database identifier assigned by repository and linked to GenBank or other repository.

"}},{"_id":"775491","name":"Sample_name","format":"","frictionlessType":"","description":"

Lab sample name

","units":{"_id":"","name":"unitless"},"type":{"_id":"960","name":"sample","description":"

unique sample identification or number; any combination of alpha numeric characters; precise definition is file dependent

"}},{"_id":"775492","name":"sample_type","format":"","frictionlessType":"","description":"

sample type and genomic analysis

","units":{"_id":"","name":"unitless"},"type":{"_id":"1741","name":"sample_descrip","description":"

text description or other traits of the sample collected

"}},{"_id":"775493","name":"host","format":"","frictionlessType":"","description":"

host taxon

","units":{"_id":"","name":"unitless"},"type":{"_id":"994","name":"taxon","description":"

taxonomic group or entity. This may be a family, class, genus, species, etc.; usually this parameter will contain a mixture of taxonomic entities.

"}},{"_id":"775494","name":"collection_date","format":"","frictionlessType":"","description":"

date collected

","units":{"_id":"","name":"unitless"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"775495","name":"geographic_location","format":"","frictionlessType":"","description":"

geographic location of collection

","units":{"_id":"","name":"unitless"},"type":{"_id":"1124","name":"site","description":"

Sampling site identification.

"}},{"_id":"775496","name":"lat","format":"","frictionlessType":"","description":"

latitude; north is positive

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"730","name":"latitude","description":"

latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes

"}},{"_id":"775497","name":"lon","format":"","frictionlessType":"","description":"

longitude; east is positive

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"731","name":"longitude","description":"

longitude, in decimal degrees, East is positive, negative denotes West; Reported in some datasets as degrees, minutes

"}},{"_id":"775498","name":"source_material_identifiers","format":"","frictionlessType":"","description":"

sample identifier

","units":{"_id":"","name":"unitless"},"type":{"_id":"960","name":"sample","description":"

unique sample identification or number; any combination of alpha numeric characters; precise definition is file dependent

"}},{"_id":"775499","name":"depth","format":"","frictionlessType":"","description":"

collection depth guage measurement

","units":{"_id":"","name":"feet seawater (fsw) or meters"},"type":{"_id":"808","name":"depth","description":"

Observation/sample depth below the sea surface. Units often reported as: meters, feet.

\nWhen used in a JGOFS/GLOBEC dataset the depth is a best estimate; usually but not always calculated from pressure; calculated either from CTD pressure using Fofonoff and Millard (1982; UNESCO Tech Paper #44) algorithm adjusted for 1980 equation of state for seawater (EOS80) or simply equivalent to nominal depth as recorded during sampling if CTD pressure was unavailable.

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